SG10201807736SA - Composition and method for stabilizing nucleic acids in biological samples - Google Patents

Composition and method for stabilizing nucleic acids in biological samples

Info

Publication number
SG10201807736SA
SG10201807736SA SG10201807736SA SG10201807736SA SG10201807736SA SG 10201807736S A SG10201807736S A SG 10201807736SA SG 10201807736S A SG10201807736S A SG 10201807736SA SG 10201807736S A SG10201807736S A SG 10201807736SA SG 10201807736S A SG10201807736S A SG 10201807736SA
Authority
SG
Singapore
Prior art keywords
biological samples
composition
nucleic acids
stabilizing
stabilizing nucleic
Prior art date
Application number
SG10201807736SA
Inventor
Hyman Chaim Birnboim
Lindsay Pozza
Hernandez Carlos Alberto Merino
Evgueni Vladimirovitch Doukhanine
Original Assignee
Dna Genotek Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dna Genotek Inc filed Critical Dna Genotek Inc
Publication of SG10201807736SA publication Critical patent/SG10201807736SA/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/7088—Compounds having three or more nucleosides or nucleotides
    • A61K31/711—Natural deoxyribonucleic acids, i.e. containing only 2'-deoxyriboses attached to adenine, guanine, cytosine or thymine and having 3'-5' phosphodiester links
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/7088—Compounds having three or more nucleosides or nucleotides
    • A61K31/713—Double-stranded nucleic acids or oligonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Epidemiology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Composition and Method for Stabilizing Nucleic Acids in Biological Samples Methods, compositions, and kits for stabilizing both human and microbial deoxyribonucleic acid (DNA) present in complex biological samples, such as feces, are disclosed. In particular, aqueous compositions for stabilizing DNA contained in biological samples at ambient temperature are disclosed, together with associated methods and kits using same. In one aspect, the compositions comprise a chelating agent present at a concentration of at least about 150 m M, and the composition has a pH of at least about 9.5. Figure: None
SG10201807736SA 2014-03-07 2015-03-06 Composition and method for stabilizing nucleic acids in biological samples SG10201807736SA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201461949692P 2014-03-07 2014-03-07
US201462057769P 2014-09-30 2014-09-30

Publications (1)

Publication Number Publication Date
SG10201807736SA true SG10201807736SA (en) 2018-10-30

Family

ID=54054312

Family Applications (2)

Application Number Title Priority Date Filing Date
SG11201607364RA SG11201607364RA (en) 2014-03-07 2015-03-06 Composition and method for stabilizing nucleic acids in biological samples
SG10201807736SA SG10201807736SA (en) 2014-03-07 2015-03-06 Composition and method for stabilizing nucleic acids in biological samples

Family Applications Before (1)

Application Number Title Priority Date Filing Date
SG11201607364RA SG11201607364RA (en) 2014-03-07 2015-03-06 Composition and method for stabilizing nucleic acids in biological samples

Country Status (18)

Country Link
US (2) US10435735B2 (en)
EP (1) EP3114225B1 (en)
JP (1) JP6670765B2 (en)
KR (1) KR102392407B1 (en)
CN (1) CN106255753B (en)
AU (1) AU2015226811B2 (en)
CA (1) CA2941764C (en)
DK (1) DK3114225T3 (en)
ES (1) ES2880310T3 (en)
IL (1) IL247662A0 (en)
MX (1) MX2016011495A (en)
PL (1) PL3114225T3 (en)
PT (1) PT3114225T (en)
RU (1) RU2717644C2 (en)
SA (2) SA521421271B1 (en)
SG (2) SG11201607364RA (en)
WO (1) WO2015131291A1 (en)
ZA (1) ZA201906139B (en)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7482116B2 (en) 2002-06-07 2009-01-27 Dna Genotek Inc. Compositions and methods for obtaining nucleic acids from sputum
IN2014MN00032A (en) 2011-06-19 2015-06-12 Abogen Inc
KR102392407B1 (en) * 2014-03-07 2022-04-29 디엔에이 제노텍 인코퍼레이티드 Composition and method for stabilizing nucleic acids in biological samples
AU2018234638B2 (en) 2017-03-15 2023-04-06 Ancestry.Com Dna, Llc Sample collection device and method
EP3691538A4 (en) 2017-10-06 2021-06-30 Ancestry.com DNA, LLC Systems, devices, and methods for sample collection
AU2018373247B2 (en) 2017-11-22 2023-11-30 Ancestry. Com Dna, Llc Sample collection kit including cap having selectively movable sleeve
US11426734B2 (en) 2017-11-22 2022-08-30 Ancestry.Com Dna, Llc Sample collection kit including cap having selectively movable sleeve
CN107955810A (en) * 2017-12-25 2018-04-24 宁波卡尔新材料科技有限公司 A kind of new animal's liver nucleic acid extraction kit and extracting method
CN111868530B (en) 2018-02-14 2024-06-11 萨琳格纳斯提克斯有限公司 Method and device for detecting analytes
CN108893523A (en) * 2018-05-31 2018-11-27 厦门安捷致善医学数据科技有限公司 A kind of fecal specimens room-temperature extender
WO2020102570A1 (en) * 2018-11-14 2020-05-22 Spectrum Solutions L.L.C. Rna preservation solution and methods of manufacture and use
WO2020174442A1 (en) 2019-02-27 2020-09-03 Ancestry.Com Dna, Llc Graphical user interface displaying relatedness based on shared dna
CN109810974B (en) * 2019-04-08 2020-11-24 珠海丽珠试剂股份有限公司 Reagent composition for extracting HIV-1 nucleic acid and method thereof
CA3152793A1 (en) * 2019-09-05 2021-03-11 The Regents Of The University Of California Noninvasive method to quantify kidney function and functional decline
CN113186185B (en) * 2020-01-14 2023-05-26 东北林业大学 Method for efficiently enriching host DNA from mammal feces
WO2021150775A1 (en) * 2020-01-21 2021-07-29 Board Of Regents Of The University Of Texas System Discriminating parkinson's disease from multiple system atrophy using alpha-synuclein pmca
CN115279901A (en) * 2020-03-11 2022-11-01 花王株式会社 Method for producing RNA derived from skin surface lipid
IE20210233A1 (en) * 2020-09-01 2023-08-16 Longhorn Vaccines & Diagnostics Llc Compositions and methods for screening biological samples
EP4244236A1 (en) * 2020-11-11 2023-09-20 Biocept, Inc. Pathogen inactivating and nucleic acid stabilization media for microorganism collection and transport
CN117157412A (en) * 2021-04-16 2023-12-01 盖立复诊断解决方案公司 Compositions and methods for storing biological samples
WO2023019124A1 (en) * 2021-08-09 2023-02-16 University Of Central Florida Research Foundation, Inc. Method to preserve nucleic acids in water samples
WO2023150879A1 (en) * 2022-02-10 2023-08-17 Dna Genotek Inc. Methods and systems for defining test sample read count limits for a range of microbial sequence reads

Family Cites Families (99)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4663161A (en) * 1985-04-22 1987-05-05 Mannino Raphael J Liposome methods and compositions
GB8614084D0 (en) * 1986-06-10 1986-07-16 Serono Diagnostics Ltd Immunoassay
US4918178A (en) 1987-10-22 1990-04-17 Wisconsin Alumni Research Foundation Test for Johne's disease
EP0338591A3 (en) 1988-04-21 1991-09-04 Microprobe Corporation Nucleic acid extraction method
JPH04187077A (en) 1990-11-22 1992-07-03 Shimadzu Corp Apparatus for extraction and purification of nucleic acid
JPH0599923A (en) 1991-10-11 1993-04-23 Nitto Denko Corp Human-hemoglobin detecting method and feces melting buffer solution used therefor
DE4204012A1 (en) * 1992-02-12 1993-08-19 Ulrich Prof Dr Zimmermann MITOGEN-FREE SUBSTANCE, THEIR PRODUCTION AND USE
DE69333289T2 (en) 1992-04-01 2004-08-26 The Johns Hopkins University School Of Medicine METHOD FOR DETERMINING MAMMAL NUCLEIC ACIDS FROM CHAIR SAMPLES AND REAGENTS REQUIRED FOR THEM
DE4321904B4 (en) 1993-07-01 2013-05-16 Qiagen Gmbh Method for chromatographic purification and separation of nucleic acid mixtures
CA2176496C (en) 1993-11-29 1999-09-28 Kathleen A. Clark Method for extracting nucleic acids from a wide range of organisms
GB9506843D0 (en) 1995-04-03 1995-05-24 Chiroscience Ltd Oxidative process and products thereof
DE19530132C2 (en) 1995-08-16 1998-07-16 Max Planck Gesellschaft Process for the purification, stabilization or isolation of nucleic acids from biological materials
GB9518156D0 (en) 1995-09-06 1995-11-08 Medical Res Council Method of isolating cells
NO954667D0 (en) 1995-11-17 1995-11-17 Dagfinn Oegreid Method for detecting Ki-ras mutations
DE19737917B4 (en) 1996-08-26 2008-11-06 InViTek Gesellschaft für Biotechnik & Biodesign mbH Method for detecting clinically relevant changes in the DNA sequence of the Ki-ras oncogene, its use and test kit for the early detection of tumors
WO1998012351A1 (en) 1996-09-19 1998-03-26 Roth W Kurt Method for purifying and eventually analyzing nucleic acids from biological test samples
AU7816898A (en) 1997-06-16 1999-01-04 Exact Laboratories, Inc. Methods for stool sample preparation
US6268136B1 (en) 1997-06-16 2001-07-31 Exact Science Corporation Methods for stool sample preparation
DE19858447A1 (en) 1997-12-18 1999-07-01 Invitek Gmbh Isolating and purifying nucleic acid
EP1053311B1 (en) 1998-02-02 2007-11-28 QIAGEN North American Holdings, Inc. Compositions and methods for using a lysing matrix for isolating dna
EP0939118A1 (en) 1998-02-20 1999-09-01 Universiteit Maastricht Method for isolating DNA and RNA from faeces
AU4934099A (en) 1998-08-04 2000-02-28 Center For Advanced Science And Technology Incubation, Ltd. Method for enzymatic amplification of nucleic acid
WO2000029618A1 (en) 1998-11-12 2000-05-25 University Of Virginia Patent Foundation Non-invasive detection of helicobacter pylori infection
DE19856064C2 (en) * 1998-12-04 2000-11-30 Invitek Gmbh Universal method for the isolation of DNA from any starting material
DE19900638C2 (en) 1999-01-11 2002-12-19 Max Planck Gesellschaft Method of isolating DNA from biological materials
JP2002537777A (en) * 1999-02-25 2002-11-12 エグザクト サイエンシーズ コーポレイション Methods for preserving DNA integrity
US20020004206A1 (en) 1999-04-09 2002-01-10 Berger Barry M. Methods of screening for disease
EP1175431A4 (en) 1999-04-30 2002-09-11 Whatman Inc Substrate including anionic detergent for purifying nucleic acid
US6270970B1 (en) * 1999-05-14 2001-08-07 Promega Corporation Mixed-bed solid phase and its use in the isolation of nucleic acids
US6586177B1 (en) 1999-09-08 2003-07-01 Exact Sciences Corporation Methods for disease detection
US6849403B1 (en) 1999-09-08 2005-02-01 Exact Sciences Corporation Apparatus and method for drug screening
AU1804401A (en) 1999-12-07 2001-06-18 Exact Sciences Corporation Apparatus and methods for drug screening based on nucleic acid analysis
US6919174B1 (en) 1999-12-07 2005-07-19 Exact Sciences Corporation Methods for disease detection
EP1252338B1 (en) 2000-02-04 2007-10-17 QIAGEN GmbH Nucleic acid isolation from stool samples and other inhibitor-rich biological materials
ES2331228T3 (en) * 2000-02-09 2009-12-28 Basf Se NEW ELONGASA GENE AND PROCESS FOR THE PREPARATION OF POLYINSATURATED FATTY ACIDS.
FR2808276B1 (en) 2000-04-26 2004-04-02 Renaud Nalin METHOD FOR INDIRECT EXTRACTION OF DNA FROM NON-CULTIVABLE ORGANISMS AND DNA LIKELY TO BE OBTAINED BY SAID PROCESS
DE10031236A1 (en) 2000-06-27 2002-01-10 Qiagen Gmbh Use of carboxylic acids and other additives in combination with cationic compounds to stabilize nucleic acids in biological materials
JP4599684B2 (en) 2000-07-26 2010-12-15 株式会社島津製作所 Nucleic acid purification method from feces
CU23095A1 (en) * 2000-11-07 2005-11-18 Cnic Ct Nac Investigaciones PROCESS FOR QUICK TYPIFICATION OF MICROORGANISMS AND REAGENT GAME EMPLOYED
US7029840B2 (en) 2000-11-15 2006-04-18 Becton, Dickinson And Company Method for preservation of cells and nucleic acid targets
US6911308B2 (en) 2001-01-05 2005-06-28 Exact Sciences Corporation Methods for detecting, grading or monitoring an H. pylori infection
DE10102338A1 (en) * 2001-01-19 2002-07-25 Basf Plant Science Gmbh New expression cassette for plant genes, useful for preparing transgenic plants that have increased production of polyunsaturated fatty acids
CA2484062A1 (en) 2001-08-20 2003-02-27 James C. Davis Dna purification and recovery from high particulate and solids samples
US7148343B2 (en) 2001-10-12 2006-12-12 Gentra Systems, Inc. Compositions and methods for using a solid support to purify RNA
US7893228B2 (en) 2001-10-12 2011-02-22 Qiagen North American Holdings, Inc. Compositions and methods for using a solid support to purify RNA
EP1438426A1 (en) 2001-10-12 2004-07-21 Gentra Systems Inc. Compositions and methods for using a solid support to purify rna
US20030109548A1 (en) * 2001-11-09 2003-06-12 Royt Paulette W. Compositions and methods of treating iron excess
JP2005525819A (en) 2002-05-17 2005-09-02 ゲーエル ビオテック ゲーエムベーハー Methods for nucleic acid extraction and purification
DE10222133A1 (en) 2002-05-17 2003-12-04 Gl Biotech Gmbh Process for nucleic acid extraction and nucleic acid purification
US20030215954A1 (en) 2002-05-17 2003-11-20 Cockerill Franklin R. Nucleic acid recovery reagents and methods
EP1506995B1 (en) 2002-05-21 2009-10-14 ARKRAY, Inc. Method of effecting lysis of acid-fast bacteria and method of performing gene amplification or detection therewith
US6852495B2 (en) 2002-06-06 2005-02-08 Shimadzu Corporation Process of extracting nucleic acid and process of simultaneously carrying out extraction and purification of nucleic acid
JP4092139B2 (en) 2002-06-06 2008-05-28 株式会社島津製作所 Nucleic acid extraction method
JP4092141B2 (en) 2002-06-07 2008-05-28 株式会社島津製作所 Nucleic acid extraction simultaneous purification method
US7482116B2 (en) 2002-06-07 2009-01-27 Dna Genotek Inc. Compositions and methods for obtaining nucleic acids from sputum
EP1391520A1 (en) 2002-08-20 2004-02-25 Becton Dickinson and Company Method for preservation of cells and nucleic acid targets
US20040157219A1 (en) 2003-02-06 2004-08-12 Jianrong Lou Chemical treatment of biological samples for nucleic acid extraction and kits therefor
FR2861085B1 (en) 2003-10-15 2006-01-27 Bertin Technologies Sa NUCLEIC ACID EXTRACTION METHOD AND APPLICATION THEREOF IN THE ANALYSIS OF THE MICROBIAL AIR POPULATION
US20050239045A1 (en) 2003-12-08 2005-10-27 Arkray Inc. Microorganism or cell collecting method, and microorganism or cell collecting implement used for the method
DE102004004901B4 (en) * 2004-01-30 2006-01-05 Johannes-Gutenberg-Universität Mainz Process for the preparation of sorbicillactone A.
US20080124714A1 (en) 2004-05-14 2008-05-29 Exact Sciences Corporation Method for Stabilizing Biological Samples for Nucleic Acid Analysis
DK1756136T3 (en) 2004-05-21 2015-01-19 Mo Bio Lab Inc KIT AND METHODS FOR REMOVING IMPURITIES FROM nucleic acids in ENVIRONMENTAL AND BIOLOGICAL SAMPLES
JP2008509226A (en) 2004-05-24 2008-03-27 ジェンボールト コーポレイション Stable protein storage and stable nucleic acid storage in recoverable format
US20050277121A1 (en) 2004-06-11 2005-12-15 Ambion, Inc. Crude biological derivatives competent for nucleic acid detection
WO2006052680A1 (en) * 2004-11-05 2006-05-18 Qiagen North American Holdings, Inc. Compositions and methods for purifying nucleic acids from stabilization reagents
WO2006053435A1 (en) * 2004-11-17 2006-05-26 Forensink Corporation Inc. Compositions for marking objects with dna and methods for marking and linking an object to its owner
JP2008532528A (en) 2005-03-16 2008-08-21 ディーエヌエー ジェノテック インク Compositions and methods for storing nucleic acids obtained from body fluids
US20070141582A1 (en) 2005-12-15 2007-06-21 Weiwei Li Method and kit for detection of early cancer or pre-cancer using blood and body fluids
JP2007248170A (en) * 2006-03-15 2007-09-27 Hitachi Ltd Container for preparing fecal suspension
WO2008021995A1 (en) 2006-08-10 2008-02-21 Cohen Ariel B Methods and compositions for detecting an analyte in a biological sample
US8080645B2 (en) * 2007-10-01 2011-12-20 Longhorn Vaccines & Diagnostics Llc Biological specimen collection/transport compositions and methods
EP2066686A4 (en) 2006-10-06 2010-07-14 Dna Genotek Inc Stabilizing compositions and methods for extraction of ribonucleic acid
WO2008089198A1 (en) 2007-01-15 2008-07-24 Greg Liang Sample collector and tester
WO2008152980A1 (en) 2007-06-12 2008-12-18 Olympus Corporation Containers for mixing biological sample with reagent and method of mixing biological sample with reagent
EP2535428B1 (en) * 2007-10-01 2015-09-09 Longhorn Vaccines and Diagnostics, LLC Biological specimen collection and transport system and methods of use
US20100255481A1 (en) 2007-10-30 2010-10-07 Olympus Corporation Method for detection of adenoma or cancer by genetic analysis
EP2218791B1 (en) 2007-11-05 2015-01-07 Eiken Kagaku Kabushiki Kaisha Method and kit for preparation of sample for use in nucleic acid amplification
EP2218792A4 (en) 2007-11-20 2010-12-08 Olympus Corp Method for preparing stool sample, solution for preparing stool sample, and kit for collecting stool
EP2255198A4 (en) 2008-02-15 2011-05-25 Mayo Foundation Detecting neoplasm
CN102016576A (en) 2008-05-02 2011-04-13 爱科来株式会社 Leukocyte analysis method and analysis reagent for use in the method
CN102027132A (en) 2008-05-12 2011-04-20 奥林巴斯株式会社 Method of processing excrement and container for processing excrement
CN102105583A (en) 2008-07-23 2011-06-22 奥林巴斯株式会社 Method for collection of nucleic acid from fecal sample, method for analysis of nucleic acid, and apparatus for treatment of fecal sample
CN102131928A (en) 2008-08-26 2011-07-20 奥林巴斯株式会社 Method for preparing fecal sample, fecal sample preparation solution, and feces collection kit
WO2010028382A2 (en) 2008-09-05 2010-03-11 Mayo Foundation For Medical Education And Research Collecting and processing complex macromolecular mixtures
US8405379B1 (en) 2008-09-18 2013-03-26 Luc Montagnier System and method for the analysis of DNA sequences in biological fluids
JPWO2010064634A1 (en) 2008-12-05 2012-05-10 オリンパス株式会社 Method for preparing stool sample, solution for preparing stool sample, and kit for collecting stool
JPWO2010064628A1 (en) 2008-12-05 2012-05-10 オリンパス株式会社 Nucleic acid-containing sample preparation method, sample preparation solution, and nucleic acid analysis method
US8427428B2 (en) 2009-03-31 2013-04-23 Key Ovation Llc Adjustable ergonomic keyboard
WO2010134245A1 (en) 2009-05-20 2010-11-25 オリンパス株式会社 Method for collection of nucleic acid derived from mammalian cell, method for analysis of nucleic acid, and kit for collection of feces
JPWO2010134246A1 (en) 2009-05-20 2012-11-08 オリンパス株式会社 Method for preparing nucleic acid-containing sample
JP2011250757A (en) 2010-06-03 2011-12-15 Olympus Corp Method for detecting nucleic acid in biological sample
CA2806734A1 (en) 2010-07-26 2012-02-09 Biomatrica, Inc. Compositions for stabilizing dna, rna and proteins in saliva and other biological samples during shipping and storage at ambient temperatures
WO2012018638A2 (en) 2010-07-26 2012-02-09 Biomatrica, Inc. Compositions for stabilizing dna, rna and proteins in blood and other biological samples during shipping and storage at ambient temperatures
NZ618935A (en) 2010-08-04 2014-03-28 Karma Medical Prod Co Ltd Compositions for fecal floral transplantation and methods for making and using them and devices for delivering them
US20120083597A1 (en) 2010-10-01 2012-04-05 Patricia Okamoto Enrichment of Low Molecular Weight DNA
US8530228B2 (en) 2010-12-28 2013-09-10 Bexmart Integrated versatile and systems preparation of specimens
US9198609B2 (en) 2011-04-19 2015-12-01 Porex Corporation Cards for sample storage and delivery comprising sintered porous plastic
US20130071847A1 (en) 2011-09-19 2013-03-21 BioPet Vet Lab Methods and materials for determining the source of waste
KR102392407B1 (en) * 2014-03-07 2022-04-29 디엔에이 제노텍 인코퍼레이티드 Composition and method for stabilizing nucleic acids in biological samples

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Publication number Publication date
DK3114225T3 (en) 2021-07-26
ES2880310T3 (en) 2021-11-24
KR20160130287A (en) 2016-11-10
AU2015226811B2 (en) 2021-07-15
BR112016020659A2 (en) 2017-08-15
WO2015131291A1 (en) 2015-09-11
ZA201906139B (en) 2023-04-26
EP3114225A4 (en) 2017-08-09
IL247662A0 (en) 2016-11-30
RU2717644C2 (en) 2020-03-24
US20200239931A1 (en) 2020-07-30
PT3114225T (en) 2021-07-26
KR102392407B1 (en) 2022-04-29
SA516371797B1 (en) 2021-06-07
CA2941764C (en) 2023-10-24
CN106255753B (en) 2020-11-06
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