BR112016004367A2 - microorganismo para produção de metionina com atividade de metionina sintase e efluxo de metionina aumentados - Google Patents

microorganismo para produção de metionina com atividade de metionina sintase e efluxo de metionina aumentados

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Publication number
BR112016004367A2
BR112016004367A2 BR112016004367A BR112016004367A BR112016004367A2 BR 112016004367 A2 BR112016004367 A2 BR 112016004367A2 BR 112016004367 A BR112016004367 A BR 112016004367A BR 112016004367 A BR112016004367 A BR 112016004367A BR 112016004367 A2 BR112016004367 A2 BR 112016004367A2
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BR
Brazil
Prior art keywords
methionine
microorganism
efflux
increased
synthase activity
Prior art date
Application number
BR112016004367A
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English (en)
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BR112016004367B1 (pt
Inventor
Vasseur Perrine
Figge Rainer
Dischert Wanda
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Metabolic Explorer Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Metabolic Explorer Sa filed Critical Metabolic Explorer Sa
Publication of BR112016004367A2 publication Critical patent/BR112016004367A2/pt
Publication of BR112016004367B1 publication Critical patent/BR112016004367B1/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/12—Methionine; Cysteine; Cystine
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/34—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Corynebacterium (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1003—Transferases (2.) transferring one-carbon groups (2.1)
    • C12N9/1007—Methyltransferases (general) (2.1.1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y201/00—Transferases transferring one-carbon groups (2.1)
    • C12Y201/01—Methyltransferases (2.1.1)
    • C12Y201/01013—Methionine synthase (2.1.1.13)

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

resumo da patente de invenã§ã£o para: ”microorganismo para produã‡ãƒo de metionina com atividade de metionina sintase e efluxo de metionina aumentados.” o presente pedido estã¡ relacionado a um microrganismo recombinante otimizado para a produã§ã£o fermentativa de metionina e/ou seus derivados, em que o referido microrganismo recombinante, a atividade de metionina sintase dependente de cobalamina e o efluxo de metionina sã£o aumentados. o pedido estã¡ tambã©m relacionado com um mã©todo para otimizar a produã§ã£o fermentativa de metionina e/ou seus derivados, compreendendo as etapas de: a. cultura de um microrganismo recombinante, em que no referido microrganismo, a atividade de metionina sintase dependente de cobalamina e o efluxo de metionina sã£o aumentados, num meio de cultura apropriado compreendendo uma fonte de carbono fermentã¡vel e uma fonte de enxofre, e b. recuperaã§ã£o de metionina e/ou seus derivados a partir do meio de cultura.
BR112016004367-7A 2013-08-30 2014-09-01 Microrganismo recombinante e método para a produção fermentativa de metionina e/ou seus derivados BR112016004367B1 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP13306185 2013-08-30
EP13306185.3 2013-08-30
PCT/EP2014/068539 WO2015028674A1 (en) 2013-08-30 2014-09-01 Microorganism for methionine production with improved methionine synthase activity and methionine efflux

Publications (2)

Publication Number Publication Date
BR112016004367A2 true BR112016004367A2 (pt) 2017-10-17
BR112016004367B1 BR112016004367B1 (pt) 2022-06-14

Family

ID=49162096

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112016004367-7A BR112016004367B1 (pt) 2013-08-30 2014-09-01 Microrganismo recombinante e método para a produção fermentativa de metionina e/ou seus derivados

Country Status (12)

Country Link
US (1) US10196658B2 (pt)
EP (1) EP3039153B1 (pt)
JP (1) JP2016529899A (pt)
KR (1) KR102270626B1 (pt)
CN (1) CN105658803B (pt)
BR (1) BR112016004367B1 (pt)
CA (1) CA2922597A1 (pt)
ES (1) ES2693789T3 (pt)
MX (1) MX2016002616A (pt)
PL (1) PL3039153T3 (pt)
RU (1) RU2016110763A (pt)
WO (1) WO2015028674A1 (pt)

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CN107109359B (zh) * 2014-09-01 2021-06-29 赢创运营有限公司 用于通过具有改进的甲硫氨酸排出的发酵生产甲硫氨酸的方法和微生物
CN108026516A (zh) 2015-08-07 2018-05-11 赢创德固赛有限公司 通过发酵的蛋白硫代羧化物依赖性l-甲硫氨酸生产
BR112018010747A8 (pt) 2015-11-27 2019-02-26 Evonik Degussa Gmbh método para a produção de l-metionina
KR20180093981A (ko) 2016-01-08 2018-08-22 에보니크 데구사 게엠베하 발효적 생산에 의해 l-메티오닌을 생산하는 방법
EP3296404A1 (en) 2016-09-15 2018-03-21 Evonik Degussa GmbH Modified microorganism for production of methionine
CN109055289B (zh) * 2018-07-27 2020-10-27 浙江工业大学 一种高产l-甲硫氨酸的重组大肠杆菌及其应用
CN112961807B (zh) * 2021-03-30 2023-01-20 中国科学院成都生物研究所 一种微生物组合物及在促进青稞种子发芽和生长上的应用

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Also Published As

Publication number Publication date
US20160177352A1 (en) 2016-06-23
WO2015028674A1 (en) 2015-03-05
EP3039153A1 (en) 2016-07-06
RU2016110763A (ru) 2017-10-05
KR20160044037A (ko) 2016-04-22
JP2016529899A (ja) 2016-09-29
BR112016004367B1 (pt) 2022-06-14
ES2693789T3 (es) 2018-12-13
EP3039153B1 (en) 2018-08-22
CA2922597A1 (en) 2015-03-05
MX2016002616A (es) 2016-06-06
PL3039153T3 (pl) 2019-02-28
CN105658803B (zh) 2020-06-23
CN105658803A (zh) 2016-06-08
KR102270626B1 (ko) 2021-06-30
US10196658B2 (en) 2019-02-05

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B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B25A Requested transfer of rights approved

Owner name: EVONIK DEGUSSA GMBH (DE)

B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B25D Requested change of name of applicant approved

Owner name: EVONIK OPERATIONS GMBH (DE)

B350 Update of information on the portal [chapter 15.35 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 01/09/2014, OBSERVADAS AS CONDICOES LEGAIS