BR112013004379A2 - produção fermentativa de ácido glicólico com um microorganismo modificado - Google Patents

produção fermentativa de ácido glicólico com um microorganismo modificado

Info

Publication number
BR112013004379A2
BR112013004379A2 BR112013004379A BR112013004379A BR112013004379A2 BR 112013004379 A2 BR112013004379 A2 BR 112013004379A2 BR 112013004379 A BR112013004379 A BR 112013004379A BR 112013004379 A BR112013004379 A BR 112013004379A BR 112013004379 A2 BR112013004379 A2 BR 112013004379A2
Authority
BR
Brazil
Prior art keywords
glycolic acid
fermentative production
modified
modified microorganism
coli strain
Prior art date
Application number
BR112013004379A
Other languages
English (en)
Inventor
Cédric Colomb
Gwénaelle Bestel-Corre
Wanda Dischert
Original Assignee
Metabolic Explorer Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Metabolic Explorer Sa filed Critical Metabolic Explorer Sa
Publication of BR112013004379A2 publication Critical patent/BR112013004379A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/42—Hydroxy-carboxylic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1077—Pentosyltransferases (2.4.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

produção fermentativa de ácido glicólico com um microorganismo modificado. a presente invenção está relacionada a um método para produção fermentativa de ácido glicólico, seus derivados e precursores, compreedendo a cultura de uma cepa escherichia coli em um meio de cultura apropriado compreendendo uma fonte de carbono e a recuperação do ácido glicólico no meio, onde a referida cepa de e.coli é modificada para aperfeiçoar a conversão de orotato em orotidina 5<39>-p. a invenção também está relacionada à cepa de e.coli modificada mostrando uma conversão aperfeiçoada de orotato em orotidina 5<39>-p e opcionalmente tendo sido adicionalmente modificada para produção de ácido glicólico aperfeiçoado.
BR112013004379A 2010-08-27 2010-08-27 produção fermentativa de ácido glicólico com um microorganismo modificado BR112013004379A2 (pt)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/IB2010/002545 WO2012025780A1 (en) 2010-08-27 2010-08-27 Improved glycolic acid fermentative production with a modified microorganism

Publications (1)

Publication Number Publication Date
BR112013004379A2 true BR112013004379A2 (pt) 2016-05-17

Family

ID=43216247

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112013004379A BR112013004379A2 (pt) 2010-08-27 2010-08-27 produção fermentativa de ácido glicólico com um microorganismo modificado

Country Status (8)

Country Link
US (1) US20130210097A1 (pt)
EP (1) EP2609208A1 (pt)
JP (1) JP2013537429A (pt)
KR (1) KR20130101030A (pt)
CN (1) CN103189517A (pt)
BR (1) BR112013004379A2 (pt)
CA (1) CA2808140A1 (pt)
WO (1) WO2012025780A1 (pt)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9902965B2 (en) 2013-11-14 2018-02-27 Scarab Genomics, Llc Bacteria with improved metabolic capacity
US10294481B2 (en) 2015-10-02 2019-05-21 Massachusetts Institute Of Technology Microbial production of renewable glycolate
CN106011185B (zh) * 2016-06-27 2019-12-17 江南大学 一种无基因敲除提高大肠杆菌中乙醇酸产率的方法
WO2018007565A1 (en) 2016-07-08 2018-01-11 Metabolic Explorer Method for the fermentative production of methionine or its hydroxy analog form by microorganisms comprising genes coding sugar phosphotransferase system (pts)
EP3354742A1 (en) * 2017-01-26 2018-08-01 Metabolic Explorer Methods and microorganisms for the production of glycolic acid and/or glyoxylic acid
EP3692159A1 (en) 2017-10-02 2020-08-12 Metabolic Explorer Method for producing organic acid salts from fermentation broth
US11384369B2 (en) 2019-02-15 2022-07-12 Braskem S.A. Microorganisms and methods for the production of glycolic acid and glycine via reverse glyoxylate shunt
CN113122489B (zh) * 2020-01-15 2022-06-14 中国科学院微生物研究所 一种产乙醇酸的重组大肠杆菌及其构建方法和应用

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US593243A (en) 1897-11-09 schneider
US5932439A (en) * 1995-11-13 1999-08-03 Monsanto Comapny Escherichia coli K-12 strains for production of recombinant proteins
TR200002113T2 (tr) 1997-11-10 2001-01-22 Wisconsin Alumni Research Foundation Lisofosfatidiletanolamin (18:1) ve lisofosfatidilinositolün yaşlanmayı geçiktirmek ve meyve olgunlaşmasını artırmak için kullanımı.
RU2282660C2 (ru) 2003-11-10 2006-08-27 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО АГРИ) Мутантная фосфорибозилпирофосфатсинтетаза, фрагмент днк, бактерия рода escherichia - продуцент l-гистидина и способ продукции l-гистидина
CN102816799B (zh) 2004-12-22 2014-06-04 纳幕尔杜邦公司 乙醇酸的酶促生产
US7326546B2 (en) 2005-03-10 2008-02-05 Ajinomoto Co., Inc. Purine-derived substance-producing bacterium and a method for producing purine-derived substance
JP2009165355A (ja) * 2006-04-28 2009-07-30 Ajinomoto Co Inc Ｌ−アミノ酸を生産する微生物及びｌ−アミノ酸の製造法
CN101437949B (zh) 2006-05-09 2012-08-22 三井化学株式会社 利用辅酶再生进行的羟基羧酸类的生产方法
US20090221045A1 (en) 2006-05-09 2009-09-03 Mitsui Chemicals, Inc. Method for producing hydroxycarboxylic acid by enhancing synthesis of coenzyme
EP2027277B1 (en) 2006-06-09 2018-07-18 Metabolic Explorer Glycolic acid production by fermentation from renewable resources
WO2007140816A1 (en) * 2006-06-09 2007-12-13 Metabolic Explorer Glycolic acid production by fermentation from renewable resources
EP2233562A1 (en) * 2009-03-24 2010-09-29 Metabolic Explorer Method for producing high amount of glycolic acid by fermentation

Also Published As

Publication number Publication date
WO2012025780A1 (en) 2012-03-01
KR20130101030A (ko) 2013-09-12
JP2013537429A (ja) 2013-10-03
CN103189517A (zh) 2013-07-03
CA2808140A1 (en) 2012-03-01
US20130210097A1 (en) 2013-08-15
EP2609208A1 (en) 2013-07-03

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B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]

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B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

Free format text: EM VIRTUDE DO ARQUIVAMENTO PUBLICADO NA RPI 2373 DE 28-06-2016 E CONSIDERANDO AUSENCIA DE MANIFESTACAO DENTRO DOS PRAZOS LEGAIS, INFORMO QUE CABE SER MANTIDO O ARQUIVAMENTO DO PEDIDO DE PATENTE, CONFORME O DISPOSTO NO ARTIGO 12, DA RESOLUCAO 113/2013.