BR112015014723A2 - método cromatográfico de isolamento e purificação de albumina de soro humano recombinante de alta pureza - Google Patents

método cromatográfico de isolamento e purificação de albumina de soro humano recombinante de alta pureza

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Publication number
BR112015014723A2
BR112015014723A2 BR112015014723A BR112015014723A BR112015014723A2 BR 112015014723 A2 BR112015014723 A2 BR 112015014723A2 BR 112015014723 A BR112015014723 A BR 112015014723A BR 112015014723 A BR112015014723 A BR 112015014723A BR 112015014723 A2 BR112015014723 A2 BR 112015014723A2
Authority
BR
Brazil
Prior art keywords
serum albumin
human serum
recombinant human
isolation
purification
Prior art date
Application number
BR112015014723A
Other languages
English (en)
Other versions
BR112015014723B1 (pt
Inventor
Shi Bo
Yang Daichang
Liu Jingru
Ou Jiquan
SHI Qianni
Original Assignee
Wuhan Healthgen Biotechnology Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=50950108&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=BR112015014723(A2) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Wuhan Healthgen Biotechnology Corp filed Critical Wuhan Healthgen Biotechnology Corp
Publication of BR112015014723A2 publication Critical patent/BR112015014723A2/pt
Publication of BR112015014723B1 publication Critical patent/BR112015014723B1/pt

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/76—Albumins
    • C07K14/765—Serum albumin, e.g. HSA
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Zoology (AREA)
  • Toxicology (AREA)
  • Peptides Or Proteins (AREA)
  • Analytical Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Chemical Kinetics & Catalysis (AREA)

Abstract

resumo descreve-se um método cromatográfico de isolamento e purificação de albumina de soro humano recombinante de alta pureza, compreendendo: realização de cromatografia de troca catiônica em albumina de soro humano recombinante, adicionando o álcool a uma solução tampão para remover a endotoxina, de modo a obter um produto primário i. sob condições de ligação, a realização da cromatografia de troca em resina composta hidrofóbica/aniônica do produto primário i para obtenção do produto intermediário ii; realização de cromatografia hidrofóbica sobre o produto intermediário ii para obtenção do produto alvo, albumina de soro humano recombinante de alta pureza. a pureza da albumina do soro humano recombinante obtida por isolamento e purificação cromatográfica com o método da presente invenção é maior que 99,9999%, e o conteúdo da endotoxina satisfaz os critérios estabelecidos pela farmacopeia chinesa. 1/1
BR112015014723-2A 2012-12-21 2013-05-09 Método cromatográfico de isolamento e purificação de albumina de soro humano recombinante de alta pureza BR112015014723B1 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
CN201210559390.7A CN103880947B (zh) 2012-12-21 2012-12-21 一种分离纯化高纯度重组人血清白蛋白的层析方法
CNPCT/CN2013/075405 2013-05-09
PCT/CN2013/075405 WO2014094406A1 (zh) 2012-12-21 2013-05-09 一种分离纯化高纯度重组人血清白蛋白的层析方法

Publications (2)

Publication Number Publication Date
BR112015014723A2 true BR112015014723A2 (pt) 2017-07-11
BR112015014723B1 BR112015014723B1 (pt) 2023-02-14

Family

ID=50950108

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112015014723-2A BR112015014723B1 (pt) 2012-12-21 2013-05-09 Método cromatográfico de isolamento e purificação de albumina de soro humano recombinante de alta pureza

Country Status (13)

Country Link
US (1) US10730926B2 (pt)
EP (2) EP3398964B1 (pt)
JP (2) JP2016501274A (pt)
KR (1) KR102026414B1 (pt)
CN (1) CN103880947B (pt)
BR (1) BR112015014723B1 (pt)
CA (1) CA2895533C (pt)
CL (1) CL2015001800A1 (pt)
DK (1) DK2937359T3 (pt)
ES (1) ES2687250T3 (pt)
PL (1) PL2937359T3 (pt)
WO (1) WO2014094406A1 (pt)
ZA (1) ZA201504507B (pt)

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CN109336967A (zh) * 2018-11-09 2019-02-15 杭州奕安济世生物药业有限公司 基于混合填料的抗体纯化方法
CN111285932B (zh) * 2018-12-10 2023-07-11 武汉禾元生物科技股份有限公司 一种从基因工程水稻种子中分离纯化重组人纤维连接蛋白的方法
CN111320699B (zh) * 2018-12-13 2023-08-29 武汉禾元生物科技股份有限公司 从基因工程水稻种子中分离纯化重组人血清白蛋白-类胰岛素融合蛋白的方法
CN111484557B (zh) * 2019-01-25 2023-07-18 武汉禾元生物科技股份有限公司 一种从基因工程水稻种子中分离纯化重组人血清白蛋白-表皮生长因子融合蛋白的方法
CN109734796B (zh) * 2019-02-01 2022-04-15 广州蕊特生物科技有限公司 一种从溶血血清中分离白蛋白的工艺
CN109776673B (zh) * 2019-02-20 2022-03-29 天津理工大学 一种适合中试量产的高纯度牛血清白蛋白纯化工艺
CN111057143A (zh) * 2019-12-31 2020-04-24 武汉理工大学 一种纳米金属氧化物纯化hsa的方法
CN113527508B (zh) * 2020-04-17 2024-01-05 上海多米瑞生物技术有限公司 一种血小板生成素拟肽-Fc融合蛋白的制备方法
CN112210002B (zh) * 2020-10-15 2022-06-10 楚天源创生物技术(长沙)有限公司 重组人血清白蛋白的纯化方法
WO2022120547A1 (zh) * 2020-12-08 2022-06-16 通化安睿特生物制药股份有限公司 纯化重组蛋白的方法
CN112521485A (zh) * 2020-12-17 2021-03-19 中国科学院过程工程研究所 一种犬血白蛋白的制备方法、利用其得到的犬血白蛋白和应用
CN113735962B (zh) * 2021-08-27 2023-11-10 常熟纳微生物科技有限公司 一种从猪血中纯化重组人血清白蛋白的方法及应用
CN115433726A (zh) * 2022-07-27 2022-12-06 武汉瀚海新酶生物科技有限公司 一种DNase I的工业化分离纯化方法
CN115894719B (zh) * 2022-11-24 2023-10-20 武汉禾元生物科技股份有限公司 一种人血清白蛋白胰岛素偶联物及其制备方法
CN115715809A (zh) * 2022-11-24 2023-02-28 武汉禾元生物科技股份有限公司 重组人血清白蛋白-药物偶联物
CN115845079B (zh) * 2022-11-24 2023-07-18 武汉禾元生物科技股份有限公司 一种重组人血清白蛋白与重组人生长激素的偶联物及其制备方法
CN116199768B (zh) * 2023-02-27 2023-10-20 武汉禾元生物科技股份有限公司 高纯度植物源重组人血清白蛋白的制备方法及其应用
CN117088962B (zh) * 2023-10-20 2024-01-09 健通(济南)生物科技有限公司 一种重组人血清白蛋白纯化过程中内毒素的去除工艺

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Also Published As

Publication number Publication date
CN103880947B (zh) 2016-01-13
KR102026414B1 (ko) 2019-09-27
JP2018087207A (ja) 2018-06-07
EP3398964B1 (en) 2020-03-18
CL2015001800A1 (es) 2016-05-27
US20150329618A1 (en) 2015-11-19
JP2016501274A (ja) 2016-01-18
EP2937359A1 (en) 2015-10-28
WO2014094406A1 (zh) 2014-06-26
DK2937359T3 (en) 2018-09-17
BR112015014723B1 (pt) 2023-02-14
ZA201504507B (en) 2016-03-30
CN103880947A (zh) 2014-06-25
EP2937359B1 (en) 2018-06-13
CA2895533C (en) 2023-04-04
EP2937359A4 (en) 2016-06-01
CA2895533A1 (en) 2014-06-26
JP6593721B2 (ja) 2019-10-23
KR20150117647A (ko) 2015-10-20
EP3398964A1 (en) 2018-11-07
US10730926B2 (en) 2020-08-04
ES2687250T3 (es) 2018-10-24
PL2937359T3 (pl) 2018-11-30

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