BR112012002151B1 - método para expressar uma desintegrina e metaloproteinase com proteína de motivo trombospondina (adamts) - Google Patents

método para expressar uma desintegrina e metaloproteinase com proteína de motivo trombospondina (adamts) Download PDF

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Publication number
BR112012002151B1
BR112012002151B1 BR112012002151-6A BR112012002151A BR112012002151B1 BR 112012002151 B1 BR112012002151 B1 BR 112012002151B1 BR 112012002151 A BR112012002151 A BR 112012002151A BR 112012002151 B1 BR112012002151 B1 BR 112012002151B1
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Prior art keywords
protein
culture medium
culture
adamts13
cell
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BR112012002151-6A
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English (en)
Portuguese (pt)
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BR112012002151A2 (pt
Inventor
Leopold Grillberger
Alexandra Spenger
Rana Grillberger
Manfred Reiter
Meinhard Hasslacher
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Baxalta GmbH
Baxalta Incorporated
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Priority to BR122021005965-9A priority Critical patent/BR122021005965B1/pt
Publication of BR112012002151A2 publication Critical patent/BR112012002151A2/pt
Publication of BR112012002151B1 publication Critical patent/BR112012002151B1/pt

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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6489Metalloendopeptidases (3.4.24)
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    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0681Cells of the genital tract; Non-germinal cells from gonads
    • C12N5/0682Cells of the female genital tract, e.g. endometrium; Non-germinal cells from ovaries, e.g. ovarian follicle cells
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    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0684Cells of the urinary tract or kidneys
    • C12N5/0686Kidney cells
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    • C12YENZYMES
    • C12Y304/00Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/24Metalloendopeptidases (3.4.24)
    • C12Y304/24087ADAMTS13 endopeptidase (3.4.24.87)
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    • C12N2500/00Specific components of cell culture medium
    • C12N2500/05Inorganic components
    • C12N2500/10Metals; Metal chelators
    • C12N2500/12Light metals, i.e. alkali, alkaline earth, Be, Al, Mg
    • C12N2500/14Calcium; Ca chelators; Calcitonin
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    • C12N2500/00Specific components of cell culture medium
    • C12N2500/05Inorganic components
    • C12N2500/10Metals; Metal chelators
    • C12N2500/20Transition metals
    • C12N2500/22Zinc; Zn chelators
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    • C12N2500/00Specific components of cell culture medium
    • C12N2500/30Organic components
    • C12N2500/38Vitamins
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    • C12N2500/00Specific components of cell culture medium
    • C12N2500/90Serum-free medium, which may still contain naturally-sourced components
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    • C12N2510/00Genetically modified cells
    • C12N2510/02Cells for production

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  • Cell Biology (AREA)
  • Urology & Nephrology (AREA)
  • Reproductive Health (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
BR112012002151-6A 2009-07-31 2010-07-30 método para expressar uma desintegrina e metaloproteinase com proteína de motivo trombospondina (adamts) BR112012002151B1 (pt)

Priority Applications (1)

Application Number Priority Date Filing Date Title
BR122021005965-9A BR122021005965B1 (pt) 2009-07-31 2010-07-30 Método para produzir uma composição de desintegrina e metaloproteinase com motivo trombospondina (adamts)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US23047709P 2009-07-31 2009-07-31
US61/230477 2009-07-31
US61/230,477 2009-07-31
PCT/US2010/044020 WO2011014838A1 (en) 2009-07-31 2010-07-30 Cell culture medium for adamts protein expression

Publications (2)

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BR112012002151A2 BR112012002151A2 (pt) 2020-03-03
BR112012002151B1 true BR112012002151B1 (pt) 2021-07-06

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BR122021005965-9A BR122021005965B1 (pt) 2009-07-31 2010-07-30 Método para produzir uma composição de desintegrina e metaloproteinase com motivo trombospondina (adamts)

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US (7) US8313926B2 (enExample)
EP (1) EP2459702B1 (enExample)
JP (5) JP5451884B2 (enExample)
KR (5) KR101756354B1 (enExample)
CN (2) CN104962539B (enExample)
AU (1) AU2010278751B2 (enExample)
BR (2) BR112012002151B1 (enExample)
CA (1) CA2769361A1 (enExample)
CO (1) CO6501191A2 (enExample)
EA (1) EA023193B1 (enExample)
ES (1) ES2583258T3 (enExample)
HR (1) HRP20160783T1 (enExample)
HU (1) HUE029110T2 (enExample)
IN (1) IN2012DN00801A (enExample)
MX (2) MX2012001261A (enExample)
NZ (1) NZ597727A (enExample)
PL (1) PL2459702T3 (enExample)
PT (1) PT2459702T (enExample)
SG (1) SG178194A1 (enExample)
SI (1) SI2459702T1 (enExample)
WO (1) WO2011014838A1 (enExample)

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KR101573972B1 (ko) 2015-12-02
CN104962539B (zh) 2019-09-17
JP2013500724A (ja) 2013-01-10
BR112012002151A2 (pt) 2020-03-03
EA023193B1 (ru) 2016-05-31
BR122021005965B1 (pt) 2022-01-25
EP2459702B1 (en) 2016-05-04
US9127265B2 (en) 2015-09-08
JP6567707B2 (ja) 2019-08-28
CN102549145B (zh) 2015-05-20
NZ597727A (en) 2013-08-30
US8759026B2 (en) 2014-06-24
EA201270224A1 (ru) 2012-09-28
JP5451884B2 (ja) 2014-03-26
PL2459702T3 (pl) 2016-12-30
SG178194A1 (en) 2012-03-29
KR20120046301A (ko) 2012-05-09
KR101808496B1 (ko) 2017-12-12
KR101574056B1 (ko) 2015-12-02
AU2010278751A1 (en) 2012-02-09
JP7083059B2 (ja) 2022-06-09
CA2769361A1 (en) 2011-02-03
US20190071660A1 (en) 2019-03-07
JP2016144471A (ja) 2016-08-12
HK1171790A1 (zh) 2013-04-05
US20130071906A1 (en) 2013-03-21
CN102549145A (zh) 2012-07-04
US20150329848A1 (en) 2015-11-19
US20110086413A1 (en) 2011-04-14
JP2019201661A (ja) 2019-11-28
JP6883071B2 (ja) 2021-06-09
WO2011014838A1 (en) 2011-02-03
KR20170081747A (ko) 2017-07-12
MX2012001261A (es) 2012-05-22
US10072254B2 (en) 2018-09-11
KR20150138425A (ko) 2015-12-09
KR101912552B1 (ko) 2018-10-26
JP2014061005A (ja) 2014-04-10
KR101756354B1 (ko) 2017-07-26
JP2018093881A (ja) 2018-06-21
CN104962539A (zh) 2015-10-07
US11254921B2 (en) 2022-02-22
ES2583258T3 (es) 2016-09-20
HUE029110T2 (en) 2017-02-28
JP2021112210A (ja) 2021-08-05
EP2459702A1 (en) 2012-06-06
PT2459702T (pt) 2016-08-02
SI2459702T1 (sl) 2016-11-30
US10724024B2 (en) 2020-07-28
US9441216B2 (en) 2016-09-13
US20200308566A1 (en) 2020-10-01
HRP20160783T1 (hr) 2016-08-12
JP5936232B2 (ja) 2016-06-22
KR20150044981A (ko) 2015-04-27
CO6501191A2 (es) 2012-08-15
JP6511007B2 (ja) 2019-05-08
US8313926B2 (en) 2012-11-20
US20140329294A1 (en) 2014-11-06
AU2010278751B2 (en) 2014-05-15
MX344970B (es) 2017-01-12
IN2012DN00801A (enExample) 2015-06-26
US20170051270A1 (en) 2017-02-23
KR20170140411A (ko) 2017-12-20

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Owner name: BAXALTA GMBH (CH) ; BAXALTA INCORPORATED (US)

B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
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