BG95362A - Химопапаин и метод за пречистването му - Google Patents

Химопапаин и метод за пречистването му

Info

Publication number
BG95362A
BG95362A BG095362A BG9536291A BG95362A BG 95362 A BG95362 A BG 95362A BG 095362 A BG095362 A BG 095362A BG 9536291 A BG9536291 A BG 9536291A BG 95362 A BG95362 A BG 95362A
Authority
BG
Bulgaria
Prior art keywords
chymopapain
papaya
peptide
proteinase
papain
Prior art date
Application number
BG095362A
Other languages
English (en)
Other versions
BG60916B1 (bg
Inventor
Alan Barrett
David Buttle
Daniel Rich
Original Assignee
The Boots Company Plc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by The Boots Company Plc filed Critical The Boots Company Plc
Publication of BG95362A publication Critical patent/BG95362A/bg
Publication of BG60916B1 publication Critical patent/BG60916B1/bg

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
    • C07K5/04—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing only normal peptide links
    • C07K5/06—Dipeptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/40—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against enzymes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
    • C07K5/04—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing only normal peptide links
    • C07K5/06—Dipeptides
    • C07K5/06008—Dipeptides with the first amino acid being neutral
    • C07K5/06017—Dipeptides with the first amino acid being neutral and aliphatic
    • C07K5/06026—Dipeptides with the first amino acid being neutral and aliphatic the side chain containing 0 or 1 carbon atom, i.e. Gly or Ala
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
    • C07K5/04—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing only normal peptide links
    • C07K5/06—Dipeptides
    • C07K5/06008—Dipeptides with the first amino acid being neutral
    • C07K5/06078—Dipeptides with the first amino acid being neutral and aromatic or cycloaliphatic
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
  • Peptides Or Proteins (AREA)
  • Steroid Compounds (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Detergent Compositions (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Compounds Of Unknown Constitution (AREA)

Abstract

Химопапаинът намира приложение в медицината за лечение на херния на междупрешленния диск. Той е практически свободен от имунологично различни протеинази, които са намерени в латекс от папайя. Химопапаинът има специфична активност спрямо варnа (1 мм) при 370с и рн 6,0, която се определя в границите от 800 до 1700 единици за мg, и съдържа по-малко от 0,2% от папайя протеиназа iii, папаин и папайя протеиназа iv. Методът включва инкубиране на воден разтвор на суров химопапаин с матрица за афинитетна хроматография с насочен център, обхващащ носеща матрица, ковалентно свързана по избор чрез пространствена връзка към n-края на пептид, обратимо инхибиращ химопапаина така, че пептидът се свързва с активната част на молекулата му, след което химопапаинът се елуира с подходящ елуент. 29 претенции
BG95362A 1989-04-28 1991-10-24 Химопапаин и метод за пречистването му BG60916B1 (bg)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB898909836A GB8909836D0 (en) 1989-04-28 1989-04-28 Therapeutic agent
PCT/EP1990/000647 WO1990013561A1 (en) 1989-04-28 1990-04-27 Therapeutic agent

Publications (2)

Publication Number Publication Date
BG95362A true BG95362A (bg) 1993-12-24
BG60916B1 BG60916B1 (bg) 1996-06-28

Family

ID=10655944

Family Applications (1)

Application Number Title Priority Date Filing Date
BG95362A BG60916B1 (bg) 1989-04-28 1991-10-24 Химопапаин и метод за пречистването му

Country Status (34)

Country Link
US (2) US5380656A (bg)
EP (1) EP0470136B1 (bg)
JP (1) JPH04506003A (bg)
KR (1) KR920701241A (bg)
CN (1) CN1047340A (bg)
AT (1) ATE107660T1 (bg)
AU (1) AU631641B2 (bg)
BG (1) BG60916B1 (bg)
CA (1) CA2056402C (bg)
DD (1) DD296960A5 (bg)
DE (1) DE69010199T2 (bg)
DK (1) DK0470136T3 (bg)
EG (1) EG19583A (bg)
ES (1) ES2055427T3 (bg)
FI (1) FI914995A0 (bg)
GB (1) GB8909836D0 (bg)
GR (1) GR1001011B (bg)
HR (1) HRP930700A2 (bg)
HU (2) HUT58345A (bg)
IE (1) IE64351B1 (bg)
IL (1) IL94227A (bg)
IN (2) IN170683B (bg)
LT (1) LT3827B (bg)
LV (1) LV10200B (bg)
MX (1) MX20503A (bg)
NO (1) NO914206L (bg)
NZ (1) NZ233475A (bg)
PL (1) PL166810B1 (bg)
PT (1) PT93921B (bg)
RO (1) RO112032B1 (bg)
RU (1) RU2074891C1 (bg)
WO (1) WO1990013561A1 (bg)
YU (1) YU87090A (bg)
ZA (1) ZA903228B (bg)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8909836D0 (en) * 1989-04-28 1989-06-14 Boots Co Plc Therapeutic agent
WO1992014696A2 (en) * 1991-02-22 1992-09-03 The Du Pont Merck Pharmaceutical Company SUBSTITUTED α-AMINOALDEHYDES AND DERIVATIVES
CA2061861A1 (en) * 1991-03-04 1992-09-05 Jonathan Duvick Plant polypeptides with inhibitory activity towards pathogenic microorganisms
CN1060519C (zh) * 1991-05-09 2001-01-10 广西亚热带作物研究所 桄榔植物蛋白酶的提取方法
AU668110B2 (en) * 1991-10-14 1996-04-26 Cash Engineering Research Pty Ltd Inlet control combination for a compressor system
US6753006B1 (en) 1993-02-22 2004-06-22 American Bioscience, Inc. Paclitaxel-containing formulations
WO1994028012A1 (en) * 1993-05-28 1994-12-08 Warner-Lambert Company Hydroxamate inhibitors of endothelin converting enzyme
US6395897B1 (en) 1999-03-02 2002-05-28 Boehringer Ingelheim Pharmaceuticals, Inc. Nitrile compounds useful as reversible inhibitors of #9 cathepsin 5
JP2002539192A (ja) * 1999-03-15 2002-11-19 アクシス・ファーマシューティカルズ・インコーポレイテッド プロテアーゼ阻害剤としての新規な化合物及び組成物
US6420364B1 (en) 1999-09-13 2002-07-16 Boehringer Ingelheim Pharmaceuticals, Inc. Compound useful as reversible inhibitors of cysteine proteases
US6703040B2 (en) 2000-01-11 2004-03-09 Intralytix, Inc. Polymer blends as biodegradable matrices for preparing biocomposites
US7935337B2 (en) 2002-12-25 2011-05-03 Hiromichi Komori Method of therapy for degenerative intervertebral discs
US7169405B2 (en) * 2003-08-06 2007-01-30 Warsaw Orthopedic, Inc. Methods and devices for the treatment of intervertebral discs
US20080069907A1 (en) * 2004-07-06 2008-03-20 Chikao Morimoto Compositions for Cancer Prevention, Treatment, or Amelioration Comprising Papaya Extract
CN1320111C (zh) * 2004-08-09 2007-06-06 王美岭 一种制备木瓜凝乳酶的方法
US20060241566A1 (en) * 2005-04-11 2006-10-26 Orthox, Llc Nucleus Extraction from Spine Intervertebral Disc
US8642059B2 (en) 2006-04-24 2014-02-04 Warsaw Orthopedic, Inc. Controlled release systems and methods for intervertebral discs
US8642060B2 (en) * 2006-04-24 2014-02-04 Warsaw Orthopedic, Inc. Controlled release systems and methods for osteal growth
US7879027B2 (en) * 2006-04-24 2011-02-01 Warsaw Orthopedic, Inc. Controlled release devices for fusion of osteal structures
US7771414B2 (en) * 2006-04-24 2010-08-10 Warsaw Orthopedic, Inc. Controlled release devices for therapeutic treatments of spinal discs
US20070265633A1 (en) * 2006-05-11 2007-11-15 Moon Jon K Implement and method to extract nucleus from spine intervertebral disc
US8257938B2 (en) * 2009-02-27 2012-09-04 Medical Diagnostic Laboratories, Llc Hemolysin and its protein fragments in sero-detection of Anaplasma phagocytophilum
JPWO2013038936A1 (ja) * 2011-09-13 2015-03-26 タカラバイオ株式会社 ペルオキシダーゼ反応の停止方法及び反応停止剤
WO2014004512A1 (en) 2012-06-25 2014-01-03 Surmodics, Inc. Bioerodable poly(etheresteramides) and medical article uses

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE270723C (bg) *
US2313875A (en) * 1940-12-17 1943-03-16 Eugene F Jansen Proteolytic enzyme process
US3320131A (en) * 1964-10-23 1967-05-16 Baxter Laboratories Inc Method for the treatment of herniation of intervertebral discs
US3558433A (en) * 1967-11-07 1971-01-26 Baxter Laboratories Inc Process for purification of chymopapain
YU40433B (en) * 1975-02-20 1986-02-28 Lek Tovarna Farmacevtskih Process for obtaining pure, proteolytically active anzymes
DE3212846A1 (de) 1981-04-13 1982-11-04 Sintokogio, Ltd., Nagoya, Aichi Apparat und verfahren zum herstellen eines kerns
US4719108A (en) * 1981-05-13 1988-01-12 Smith Laboratories, Inc. Chymopapain composition and method for its use
DE3278934D1 (en) * 1981-05-13 1988-09-29 Flint Lab Inc Improved chymopapain and method for its production and use
US4439423A (en) * 1981-05-13 1984-03-27 Smith Laboratories, Inc. Chymopapain and method for its use
US4374926A (en) * 1981-05-13 1983-02-22 Smith Laboratories, Inc. Method for the production of improved chymopapain
GB2124233B (en) * 1982-07-19 1985-09-18 Nat Res Dev Synthetic peptides and their preparation
GB2156821A (en) * 1984-04-03 1985-10-16 Simmons James W Process for purifying chymopapain
HU196624B (en) * 1986-12-08 1988-12-28 Chinoin Gyogyszer Es Vegyeszet Process for producing chymopapain with high specific activity
GB8909836D0 (en) * 1989-04-28 1989-06-14 Boots Co Plc Therapeutic agent

Also Published As

Publication number Publication date
AU5547290A (en) 1990-11-29
BG60916B1 (bg) 1996-06-28
AU631641B2 (en) 1992-12-03
GR1001011B (el) 1993-03-31
ATE107660T1 (de) 1994-07-15
FI914995A0 (fi) 1991-10-23
HRP930700A2 (en) 1995-06-30
IL94227A0 (en) 1991-01-31
LT3827B (en) 1996-03-25
KR920701241A (ko) 1992-08-11
DE69010199T2 (de) 1994-10-13
CA2056402A1 (en) 1990-10-29
PT93921B (pt) 1996-10-31
IE64351B1 (en) 1995-07-26
EP0470136A1 (en) 1992-02-12
RO112032B1 (ro) 1997-04-30
NO914206D0 (no) 1991-10-25
PL166810B1 (pl) 1995-06-30
NO914206L (no) 1991-12-17
EG19583A (en) 1995-09-30
JPH04506003A (ja) 1992-10-22
WO1990013561A1 (en) 1990-11-15
LV10200A (lv) 1994-10-20
ZA903228B (en) 1990-12-28
US5468480A (en) 1995-11-21
CA2056402C (en) 1999-11-16
ES2055427T3 (es) 1994-08-16
IL94227A (en) 1994-12-29
IE901538L (en) 1990-10-28
MX20503A (es) 1993-12-01
IN173230B (bg) 1994-03-12
YU87090A (sh) 1992-09-07
HU211746A9 (en) 1995-12-28
DE69010199D1 (de) 1994-07-28
PT93921A (pt) 1990-11-20
DK0470136T3 (da) 1994-08-01
LV10200B (en) 1995-04-20
HU903342D0 (en) 1992-01-28
GB8909836D0 (en) 1989-06-14
EP0470136B1 (en) 1994-06-22
RU2074891C1 (ru) 1997-03-10
LTIP1645A (en) 1995-07-25
US5380656A (en) 1995-01-10
GR900100315A (en) 1991-09-27
DD296960A5 (de) 1991-12-19
HUT58345A (en) 1992-02-28
NZ233475A (en) 1991-10-25
CN1047340A (zh) 1990-11-28
IN170683B (bg) 1992-05-02

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