AU713635B2 - Process for chromatographic separation of peptides and nucleic acid, and new high affinity ion exchange matrix - Google Patents

Process for chromatographic separation of peptides and nucleic acid, and new high affinity ion exchange matrix Download PDF

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Publication number
AU713635B2
AU713635B2 AU18180/97A AU1818097A AU713635B2 AU 713635 B2 AU713635 B2 AU 713635B2 AU 18180/97 A AU18180/97 A AU 18180/97A AU 1818097 A AU1818097 A AU 1818097A AU 713635 B2 AU713635 B2 AU 713635B2
Authority
AU
Australia
Prior art keywords
chohch
ligand
anion
anion exchanger
group
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
AU18180/97A
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English (en)
Other versions
AU1818097A (en
Inventor
Makonnen Belew
Rolf Berglund
Jan Bergstrom
Lennart Soderberg
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Global Life Sciences Solutions USA LLC
Original Assignee
Amersham Pharmacia Biotech AB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amersham Pharmacia Biotech AB filed Critical Amersham Pharmacia Biotech AB
Publication of AU1818097A publication Critical patent/AU1818097A/en
Application granted granted Critical
Publication of AU713635B2 publication Critical patent/AU713635B2/en
Assigned to GE HEALTHCARE BIO-SCIENCES AB reassignment GE HEALTHCARE BIO-SCIENCES AB Request to Amend Deed and Register Assignors: AMERSHAM PHARMACIA BIOTECH AB
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
    • B01D15/361—Ion-exchange
    • B01D15/363—Anion-exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J41/00—Anion exchange; Use of material as anion exchangers; Treatment of material for improving the anion exchange properties
    • B01J41/04—Processes using organic exchangers
    • B01J41/07—Processes using organic exchangers in the weakly basic form
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J41/00—Anion exchange; Use of material as anion exchangers; Treatment of material for improving the anion exchange properties
    • B01J41/20—Anion exchangers for chromatographic processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2220/00—Aspects relating to sorbent materials
    • B01J2220/50—Aspects relating to the use of sorbent or filter aid materials
    • B01J2220/54—Sorbents specially adapted for analytical or investigative chromatography

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Peptides Or Proteins (AREA)
AU18180/97A 1996-02-19 1997-02-14 Process for chromatographic separation of peptides and nucleic acid, and new high affinity ion exchange matrix Ceased AU713635B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
SE9600590 1996-02-19
SE9600590A SE9600590D0 (sv) 1996-02-19 1996-02-19 Sätt för kromatografisk separation av peptider och nukleinsyra samt ny högaffin jonbytesmatris
PCT/SE1997/000237 WO1997029825A1 (en) 1996-02-19 1997-02-14 Process for chromatographic separation of peptides and nucleic acid, and new high affinity ion exchange matrix

Publications (2)

Publication Number Publication Date
AU1818097A AU1818097A (en) 1997-09-02
AU713635B2 true AU713635B2 (en) 1999-12-09

Family

ID=20401430

Family Applications (1)

Application Number Title Priority Date Filing Date
AU18180/97A Ceased AU713635B2 (en) 1996-02-19 1997-02-14 Process for chromatographic separation of peptides and nucleic acid, and new high affinity ion exchange matrix

Country Status (8)

Country Link
US (1) US6090288A (enExample)
EP (1) EP0888157B1 (enExample)
JP (1) JP4422797B2 (enExample)
AT (1) ATE267037T1 (enExample)
AU (1) AU713635B2 (enExample)
DE (1) DE69729182T2 (enExample)
SE (1) SE9600590D0 (enExample)
WO (1) WO1997029825A1 (enExample)

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DE19520398B4 (de) 1995-06-08 2009-04-16 Roche Diagnostics Gmbh Magnetisches Pigment
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US6914137B2 (en) * 1997-12-06 2005-07-05 Dna Research Innovations Limited Isolation of nucleic acids
DE69805649T2 (de) * 1997-12-06 2002-12-05 Dna Res Innovations Ltd Isolierung von nukleinsäuren
US7078224B1 (en) 1999-05-14 2006-07-18 Promega Corporation Cell concentration and lysate clearance using paramagnetic particles
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WO2000034521A1 (en) * 1998-12-08 2000-06-15 Boston Probes, Inc. Methods, kits and compositions for the identification of nucleic acids electrostatically bound to matrices
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US6310199B1 (en) 1999-05-14 2001-10-30 Promega Corporation pH dependent ion exchange matrix and method of use in the isolation of nucleic acids
US6270970B1 (en) 1999-05-14 2001-08-07 Promega Corporation Mixed-bed solid phase and its use in the isolation of nucleic acids
SE9904197D0 (sv) * 1999-11-22 1999-11-22 Amersham Pharm Biotech Ab A method for anion exchange adsorption on matrices carrying mixed mode ligands
SE9904272D0 (sv) * 1999-11-25 1999-11-25 Amersham Pharm Biotech Ab A method for selective removal of a substance from samples containing compounds having nucleic acid structure
GB9927904D0 (en) * 1999-11-25 2000-01-26 Amersham Pharm Biotech Ab A method fro obtaining a nucleic acid variant
AU2001231527A1 (en) * 2000-02-08 2001-08-20 Upfront Chromatography A/S Novel resin materials and their use for recovering proteins or peptides
US20020194901A1 (en) * 2000-12-19 2002-12-26 Showa Denko K.K. Porous polymer particle, alkali-resistant anion exchanger, producing method thereof, column for ion chromatography, and method for measuring anions
US20030066336A1 (en) * 2000-12-19 2003-04-10 Showa Denko K.K. Method for measuring anions
SE0004932D0 (sv) * 2000-12-31 2000-12-31 Apbiotech Ab A method for mixed mode adsorption and mixed mode adsorbents
DE60202240T2 (de) * 2001-05-21 2005-12-15 Fuji Photo Film Co., Ltd., Minami-Ashigara Vorrichtung zur Durchführung biochemischer Analysen und Verfahren zu ihrer Herstellung
SE0103084D0 (sv) * 2001-09-14 2001-09-14 Amersham Pharm Biotech Ab Generation of ion exchange media
GB0127809D0 (en) * 2001-11-20 2002-01-09 Dna Res Innovations Ltd Purification of nucleic acid
AU2003202026A1 (en) 2002-01-16 2003-09-02 Dynal Biotech Asa Method for isolating nucleic acids and protein from a single sample
WO2003074571A2 (en) * 2002-02-28 2003-09-12 Keck Graduate Institute Cationic polyelectrolytes in biomolecule purification and analysis
US20060035224A1 (en) * 2002-03-21 2006-02-16 Johansen Jack T Purification methods for oligonucleotides and their analogs
GB0207975D0 (en) * 2002-04-05 2002-05-15 Genovision As Isolating nucleic acid
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US6989442B2 (en) * 2002-07-12 2006-01-24 Sirna Therapeutics, Inc. Deprotection and purification of oligonucleotides and their derivatives
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SE0302911D0 (sv) * 2003-10-31 2003-10-31 Amersham Biosciences Ab Novel separation matrix
US20050106589A1 (en) * 2003-11-17 2005-05-19 Hashem Akhavan-Tafti Compositions and methods for releasing nucleic acids from solid phase binding materials
SE0303532D0 (sv) * 2003-12-23 2003-12-23 Amersham Biosciences Ab Purification of immunoglobulins
US7479222B2 (en) * 2004-02-05 2009-01-20 Millipore Corporation Porous adsorptive or chromatographic media
EP1693108A1 (en) * 2004-12-04 2006-08-23 MERCK PATENT GmbH Mixed-modal anion-exchange type separation material
KR100668337B1 (ko) 2005-05-21 2007-01-12 삼성전자주식회사 단백질에 비하여 핵산에 대한 결합력이 선택적으로 높은ｐＨ 의존성 이온 교환물질, 그가 고정화되어 있는 고체기판, 및 상기 물질 및 고체 기판을 이용하여 핵산을분리하는 방법
CN101258175B (zh) 2005-08-03 2011-11-16 默克专利股份公司 亲水交联聚合物
EP1754534A1 (de) 2005-08-03 2007-02-21 MERCK PATENT GmbH Hydrophiles vernetztes Polymer
GB2445442A (en) * 2006-09-26 2008-07-09 Ge Healthcare Bio Sciences Nucleic acid purification using anion exchange
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US9433922B2 (en) 2007-08-14 2016-09-06 Emd Millipore Corporation Media for membrane ion exchange chromatography based on polymeric primary amines, sorption device containing that media, and chromatography scheme and purification method using the same
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CN103314290B (zh) * 2011-01-12 2015-07-01 积水医疗株式会社 核酸链的分离方法
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CN104768536B (zh) * 2012-09-17 2019-03-29 格雷斯公司 官能化微粒状载体材料和其制备和使用方法
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Citations (3)

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Patent Citations (3)

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EP0276138A2 (en) * 1987-01-21 1988-07-27 E.I. Du Pont De Nemours And Company Chromatographic supports for separation of oligonucleotides
EP0416748A1 (en) * 1989-08-07 1991-03-13 J.T. Baker Inc. Quaternised pei silica solid supports for chromatography
WO1992011088A1 (en) * 1990-12-21 1992-07-09 Pharmacia Lkb Biotechnology Ab Anion exchanger

Also Published As

Publication number Publication date
DE69729182D1 (de) 2004-06-24
EP0888157A1 (en) 1999-01-07
SE9600590D0 (sv) 1996-02-19
ATE267037T1 (de) 2004-06-15
JP4422797B2 (ja) 2010-02-24
JP2000504625A (ja) 2000-04-18
AU1818097A (en) 1997-09-02
WO1997029825A1 (en) 1997-08-21
DE69729182T2 (de) 2005-01-27
EP0888157B1 (en) 2004-05-19
US6090288A (en) 2000-07-18

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