ATE267037T1 - Prozess zur chromatographischen trennung von peptiden und nucleinsaure und neue ionentauschermatrix mit hoher affinitat - Google Patents
Prozess zur chromatographischen trennung von peptiden und nucleinsaure und neue ionentauschermatrix mit hoher affinitatInfo
- Publication number
- ATE267037T1 ATE267037T1 AT97903706T AT97903706T ATE267037T1 AT E267037 T1 ATE267037 T1 AT E267037T1 AT 97903706 T AT97903706 T AT 97903706T AT 97903706 T AT97903706 T AT 97903706T AT E267037 T1 ATE267037 T1 AT E267037T1
- Authority
- AT
- Austria
- Prior art keywords
- pct
- peptides
- ion exchange
- anion exchanger
- high affinity
- Prior art date
Links
- 239000011159 matrix material Substances 0.000 title abstract 2
- 108090000765 processed proteins & peptides Proteins 0.000 title abstract 2
- 239000002253 acid Substances 0.000 title 1
- 150000007513 acids Chemical class 0.000 title 1
- 238000013375 chromatographic separation Methods 0.000 title 1
- 238000005342 ion exchange Methods 0.000 title 1
- 238000000034 method Methods 0.000 title 1
- 102000004196 processed proteins & peptides Human genes 0.000 title 1
- 150000001450 anions Chemical class 0.000 abstract 3
- 108010058846 Ovalbumin Proteins 0.000 abstract 2
- 238000010828 elution Methods 0.000 abstract 2
- 239000003446 ligand Substances 0.000 abstract 2
- 229940092253 ovalbumin Drugs 0.000 abstract 2
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 abstract 2
- 125000000467 secondary amino group Chemical group [H]N([*:1])[*:2] 0.000 abstract 2
- 125000001302 tertiary amino group Chemical group 0.000 abstract 2
- 101710143935 Beta-lactoglobulin-1 Proteins 0.000 abstract 1
- 101710143930 Beta-lactoglobulin-2 Proteins 0.000 abstract 1
- OAKJQQAXSVQMHS-UHFFFAOYSA-N Hydrazine Chemical compound NN OAKJQQAXSVQMHS-UHFFFAOYSA-N 0.000 abstract 1
- 102000004338 Transferrin Human genes 0.000 abstract 1
- 108090000901 Transferrin Proteins 0.000 abstract 1
- 125000004429 atom Chemical group 0.000 abstract 1
- 229910052799 carbon Inorganic materials 0.000 abstract 1
- 125000004432 carbon atom Chemical group C* 0.000 abstract 1
- 230000000052 comparative effect Effects 0.000 abstract 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 abstract 1
- 150000002500 ions Chemical class 0.000 abstract 1
- 108020004707 nucleic acids Proteins 0.000 abstract 1
- 102000039446 nucleic acids Human genes 0.000 abstract 1
- 150000007523 nucleic acids Chemical class 0.000 abstract 1
- 229920000620 organic polymer Polymers 0.000 abstract 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
- B01D15/361—Ion-exchange
- B01D15/363—Anion-exchange
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J41/00—Anion exchange; Use of material as anion exchangers; Treatment of material for improving the anion exchange properties
- B01J41/04—Processes using organic exchangers
- B01J41/07—Processes using organic exchangers in the weakly basic form
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J41/00—Anion exchange; Use of material as anion exchangers; Treatment of material for improving the anion exchange properties
- B01J41/20—Anion exchangers for chromatographic processes
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/18—Ion-exchange chromatography
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2220/00—Aspects relating to sorbent materials
- B01J2220/50—Aspects relating to the use of sorbent or filter aid materials
- B01J2220/54—Sorbents specially adapted for analytical or investigative chromatography
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biochemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SE9600590A SE9600590D0 (sv) | 1996-02-19 | 1996-02-19 | Sätt för kromatografisk separation av peptider och nukleinsyra samt ny högaffin jonbytesmatris |
| PCT/SE1997/000237 WO1997029825A1 (en) | 1996-02-19 | 1997-02-14 | Process for chromatographic separation of peptides and nucleic acid, and new high affinity ion exchange matrix |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE267037T1 true ATE267037T1 (de) | 2004-06-15 |
Family
ID=20401430
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT97903706T ATE267037T1 (de) | 1996-02-19 | 1997-02-14 | Prozess zur chromatographischen trennung von peptiden und nucleinsaure und neue ionentauschermatrix mit hoher affinitat |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US6090288A (enExample) |
| EP (1) | EP0888157B1 (enExample) |
| JP (1) | JP4422797B2 (enExample) |
| AT (1) | ATE267037T1 (enExample) |
| AU (1) | AU713635B2 (enExample) |
| DE (1) | DE69729182T2 (enExample) |
| SE (1) | SE9600590D0 (enExample) |
| WO (1) | WO1997029825A1 (enExample) |
Families Citing this family (70)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB9425138D0 (en) | 1994-12-12 | 1995-02-08 | Dynal As | Isolation of nucleic acid |
| KR100463475B1 (ko) | 1995-06-08 | 2005-06-22 | 로셰 디아그노스틱스 게엠베하 | 자기성피그먼트 |
| DE19520398B4 (de) | 1995-06-08 | 2009-04-16 | Roche Diagnostics Gmbh | Magnetisches Pigment |
| US6027945A (en) | 1997-01-21 | 2000-02-22 | Promega Corporation | Methods of isolating biological target materials using silica magnetic particles |
| SE9700769D0 (sv) | 1997-03-04 | 1997-03-04 | Pharmacia Biotech Ab | Matriser för separation och separation som utnyttjar matriserna |
| DE19736366A1 (de) * | 1997-08-21 | 1999-02-25 | Ernst Prof Dr Bayer | Verfahren zur Isolierung von anionischen organischen Verbindungen aus wäßrigen Systemen mit kationischen Polymernanopartikeln |
| US6914137B2 (en) * | 1997-12-06 | 2005-07-05 | Dna Research Innovations Limited | Isolation of nucleic acids |
| DE69805649T2 (de) * | 1997-12-06 | 2002-12-05 | Dna Res Innovations Ltd | Isolierung von nukleinsäuren |
| US7078224B1 (en) | 1999-05-14 | 2006-07-18 | Promega Corporation | Cell concentration and lysate clearance using paramagnetic particles |
| SE9802214D0 (sv) * | 1998-06-18 | 1998-06-18 | Amersham Pharm Biotech Ab | Jonbytare och användning därav |
| WO2000034521A1 (en) * | 1998-12-08 | 2000-06-15 | Boston Probes, Inc. | Methods, kits and compositions for the identification of nucleic acids electrostatically bound to matrices |
| AU2862100A (en) * | 1999-01-27 | 2000-08-18 | Folim G. Halaka | Materials and methods for the purification of polyelectrolytes |
| US6310199B1 (en) | 1999-05-14 | 2001-10-30 | Promega Corporation | pH dependent ion exchange matrix and method of use in the isolation of nucleic acids |
| US6270970B1 (en) | 1999-05-14 | 2001-08-07 | Promega Corporation | Mixed-bed solid phase and its use in the isolation of nucleic acids |
| SE9904197D0 (sv) * | 1999-11-22 | 1999-11-22 | Amersham Pharm Biotech Ab | A method for anion exchange adsorption on matrices carrying mixed mode ligands |
| SE9904272D0 (sv) * | 1999-11-25 | 1999-11-25 | Amersham Pharm Biotech Ab | A method for selective removal of a substance from samples containing compounds having nucleic acid structure |
| GB9927904D0 (en) * | 1999-11-25 | 2000-01-26 | Amersham Pharm Biotech Ab | A method fro obtaining a nucleic acid variant |
| AU2001231527A1 (en) * | 2000-02-08 | 2001-08-20 | Upfront Chromatography A/S | Novel resin materials and their use for recovering proteins or peptides |
| US20020194901A1 (en) * | 2000-12-19 | 2002-12-26 | Showa Denko K.K. | Porous polymer particle, alkali-resistant anion exchanger, producing method thereof, column for ion chromatography, and method for measuring anions |
| US20030066336A1 (en) * | 2000-12-19 | 2003-04-10 | Showa Denko K.K. | Method for measuring anions |
| SE0004932D0 (sv) * | 2000-12-31 | 2000-12-31 | Apbiotech Ab | A method for mixed mode adsorption and mixed mode adsorbents |
| DE60202240T2 (de) * | 2001-05-21 | 2005-12-15 | Fuji Photo Film Co., Ltd., Minami-Ashigara | Vorrichtung zur Durchführung biochemischer Analysen und Verfahren zu ihrer Herstellung |
| SE0103084D0 (sv) * | 2001-09-14 | 2001-09-14 | Amersham Pharm Biotech Ab | Generation of ion exchange media |
| GB0127809D0 (en) * | 2001-11-20 | 2002-01-09 | Dna Res Innovations Ltd | Purification of nucleic acid |
| AU2003202026A1 (en) | 2002-01-16 | 2003-09-02 | Dynal Biotech Asa | Method for isolating nucleic acids and protein from a single sample |
| WO2003074571A2 (en) * | 2002-02-28 | 2003-09-12 | Keck Graduate Institute | Cationic polyelectrolytes in biomolecule purification and analysis |
| US20060035224A1 (en) * | 2002-03-21 | 2006-02-16 | Johansen Jack T | Purification methods for oligonucleotides and their analogs |
| GB0207975D0 (en) * | 2002-04-05 | 2002-05-15 | Genovision As | Isolating nucleic acid |
| GB0212826D0 (en) | 2002-05-31 | 2002-07-10 | Dna Res Innovations Ltd | Materials and methods relating to polyions and substance delivery |
| US6989442B2 (en) * | 2002-07-12 | 2006-01-24 | Sirna Therapeutics, Inc. | Deprotection and purification of oligonucleotides and their derivatives |
| WO2004078349A1 (en) * | 2003-03-05 | 2004-09-16 | Amersham Biosciences Ab | A method of preparing multi-modal anion-exchange ligands |
| SE0302911D0 (sv) * | 2003-10-31 | 2003-10-31 | Amersham Biosciences Ab | Novel separation matrix |
| US20050106589A1 (en) * | 2003-11-17 | 2005-05-19 | Hashem Akhavan-Tafti | Compositions and methods for releasing nucleic acids from solid phase binding materials |
| SE0303532D0 (sv) * | 2003-12-23 | 2003-12-23 | Amersham Biosciences Ab | Purification of immunoglobulins |
| US7479222B2 (en) * | 2004-02-05 | 2009-01-20 | Millipore Corporation | Porous adsorptive or chromatographic media |
| EP1693108A1 (en) * | 2004-12-04 | 2006-08-23 | MERCK PATENT GmbH | Mixed-modal anion-exchange type separation material |
| KR100668337B1 (ko) | 2005-05-21 | 2007-01-12 | 삼성전자주식회사 | 단백질에 비하여 핵산에 대한 결합력이 선택적으로 높은pH 의존성 이온 교환물질, 그가 고정화되어 있는 고체기판, 및 상기 물질 및 고체 기판을 이용하여 핵산을분리하는 방법 |
| CN101258175B (zh) | 2005-08-03 | 2011-11-16 | 默克专利股份公司 | 亲水交联聚合物 |
| EP1754534A1 (de) | 2005-08-03 | 2007-02-21 | MERCK PATENT GmbH | Hydrophiles vernetztes Polymer |
| GB2445442A (en) * | 2006-09-26 | 2008-07-09 | Ge Healthcare Bio Sciences | Nucleic acid purification using anion exchange |
| GB2445441B (en) * | 2006-09-26 | 2010-06-30 | Ge Healthcare Bio Sciences | Nucleic acid purification method |
| GB2443505B (en) | 2006-09-26 | 2008-12-31 | Ge Healthcare Bio Sciences | Nucleic acid purification method |
| US9433922B2 (en) | 2007-08-14 | 2016-09-06 | Emd Millipore Corporation | Media for membrane ion exchange chromatography based on polymeric primary amines, sorption device containing that media, and chromatography scheme and purification method using the same |
| DE202008017854U1 (de) | 2007-08-14 | 2010-08-19 | Millipore Corp., Billerica | Mittel zur Ionenaustausch-Membranchromatographie auf der Basis von primären polymerischen Aminen, Sorptionsvorrichtung mit diesem Mittel, Chromatographieverfahren und Reinigungsverfahren unter dessen Verwendung |
| US20090130738A1 (en) * | 2007-11-19 | 2009-05-21 | Mikhail Kozlov | Media for membrane ion exchange chromatography |
| EP2250182B1 (en) * | 2008-02-07 | 2015-12-02 | GE Healthcare Bio-Sciences Corp. | Isolation of dna, rna and protein from a single sample |
| CA2720615C (en) | 2008-04-08 | 2016-06-07 | Bio-Rad Laboratories, Inc. | Chromatography purification of antibodies |
| WO2009145722A1 (en) * | 2008-05-30 | 2009-12-03 | Ge Healthcare Bio-Sciences Ab | Separation method utilizing polyallylamine ligands |
| WO2012096329A1 (ja) | 2011-01-12 | 2012-07-19 | 積水メディカル株式会社 | 一塩基多型の検出方法 |
| CN103314290B (zh) * | 2011-01-12 | 2015-07-01 | 积水医疗株式会社 | 核酸链的分离方法 |
| JP5734320B2 (ja) * | 2011-02-10 | 2015-06-17 | 積水メディカル株式会社 | イオン交換クロマトグラフィー用充填剤及び核酸鎖の分離検出方法 |
| EP2822687B1 (en) * | 2012-03-08 | 2019-11-27 | Bio-rad Laboratories, Inc. | Anionic exchange-hydrophobic mixed mode |
| US9169304B2 (en) | 2012-05-01 | 2015-10-27 | Pfenex Inc. | Process for purifying recombinant Plasmodium falciparum circumsporozoite protein |
| CN104768536B (zh) * | 2012-09-17 | 2019-03-29 | 格雷斯公司 | 官能化微粒状载体材料和其制备和使用方法 |
| CN104968403A (zh) | 2012-09-17 | 2015-10-07 | 格雷斯公司 | 色谱介质和装置 |
| WO2014084790A1 (en) * | 2012-11-29 | 2014-06-05 | Jerker Porath | Superhydroxylated adsorbents and uses thereof |
| US11229896B2 (en) | 2014-01-16 | 2022-01-25 | W.R. Grace & Co.—Conn. | Affinity chromatography media and chromatography devices |
| CN114917884A (zh) | 2014-03-14 | 2022-08-19 | 思拓凡生物工艺研发有限公司 | 用于生物颗粒纯化的分离基质 |
| ES2929099T3 (es) | 2014-05-02 | 2022-11-24 | Grace W R & Co | Material de soporte funcionalizado y métodos de fabricación y uso de material de soporte funcionalizado |
| CN106459139A (zh) | 2014-05-28 | 2017-02-22 | 新加坡科技研究局 | 病毒减少方法 |
| EP3286172B1 (en) | 2015-04-23 | 2019-06-12 | Constellation Pharmaceuticals, Inc. | Lsd1 inhibitors and uses thereof |
| PL3302784T3 (pl) | 2015-06-05 | 2022-01-17 | W.R. Grace & Co.-Conn. | Adsorbentowe środki klarujące do bioprzetwarzania oraz sposoby ich wytwarzania i stosowania |
| JP7068316B2 (ja) * | 2016-09-15 | 2022-05-16 | クラヴェゴ ゲーエムベーハー アンド コー カーゲー | 巨大分子の精製のための高分子メッシュの利用 |
| HUE063848T2 (hu) | 2016-10-26 | 2024-02-28 | Constellation Pharmaceuticals Inc | LSD1 gátlók és gyógyászati alkalmazásaik |
| JP7045373B2 (ja) * | 2017-06-22 | 2022-03-31 | 昭和電工株式会社 | オリゴヌクレオチドの混合物の分離分析方法 |
| CN111683976B (zh) | 2018-02-05 | 2022-11-18 | 生物辐射实验室股份有限公司 | 具有阴离子交换-疏水混合模式配体的色谱树脂 |
| CN115318256A (zh) | 2018-03-08 | 2022-11-11 | 生物辐射实验室股份有限公司 | 阴离子交换-疏水混合模式色谱树脂 |
| JP7717627B2 (ja) | 2019-09-05 | 2025-08-04 | バイオ-ラッド・ラボラトリーズ・インコーポレーテッド | アニオン交換-疎水性混合モードクロマトグラフィー樹脂 |
| EP4070102A1 (en) | 2019-12-05 | 2022-10-12 | Waters Technologies Corporation | Polyphosphonic acids for improving recovery and minimizing system loss |
| US20220017887A1 (en) * | 2020-07-14 | 2022-01-20 | Waters Technologies Corporation | Compositions, kits and methods useful for separating oligonucleotides from matrix components |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4767670A (en) * | 1987-01-21 | 1988-08-30 | E. I. Du Pont De Nemours And Company | Chromatographic supports for separation of oligonucleotides |
| SE8902315D0 (sv) * | 1989-06-27 | 1989-06-27 | Pharmacia Ab | Anjonbytare |
| EP0416748B1 (en) * | 1989-08-07 | 1994-03-30 | J.T. Baker Inc. | Quaternised pei silica solid supports for chromatography |
| US5087359A (en) * | 1989-08-07 | 1992-02-11 | J. T. Baker Inc. | Quaternized PEI silica solid supports for chromatography |
| SE9004129D0 (sv) * | 1990-12-21 | 1990-12-21 | Pharmacia Lkb Biotech | Anion exchanger |
-
1996
- 1996-02-19 SE SE9600590A patent/SE9600590D0/xx unknown
-
1997
- 1997-02-14 EP EP97903706A patent/EP0888157B1/en not_active Expired - Lifetime
- 1997-02-14 WO PCT/SE1997/000237 patent/WO1997029825A1/en not_active Ceased
- 1997-02-14 DE DE69729182T patent/DE69729182T2/de not_active Expired - Lifetime
- 1997-02-14 AT AT97903706T patent/ATE267037T1/de not_active IP Right Cessation
- 1997-02-14 AU AU18180/97A patent/AU713635B2/en not_active Ceased
- 1997-02-14 JP JP52927797A patent/JP4422797B2/ja not_active Expired - Lifetime
- 1997-02-14 US US09/117,678 patent/US6090288A/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| DE69729182D1 (de) | 2004-06-24 |
| EP0888157A1 (en) | 1999-01-07 |
| SE9600590D0 (sv) | 1996-02-19 |
| JP4422797B2 (ja) | 2010-02-24 |
| JP2000504625A (ja) | 2000-04-18 |
| AU1818097A (en) | 1997-09-02 |
| WO1997029825A1 (en) | 1997-08-21 |
| DE69729182T2 (de) | 2005-01-27 |
| EP0888157B1 (en) | 2004-05-19 |
| US6090288A (en) | 2000-07-18 |
| AU713635B2 (en) | 1999-12-09 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |