ATE267037T1 - Prozess zur chromatographischen trennung von peptiden und nucleinsaure und neue ionentauschermatrix mit hoher affinitat - Google Patents

Prozess zur chromatographischen trennung von peptiden und nucleinsaure und neue ionentauschermatrix mit hoher affinitat

Info

Publication number
ATE267037T1
ATE267037T1 AT97903706T AT97903706T ATE267037T1 AT E267037 T1 ATE267037 T1 AT E267037T1 AT 97903706 T AT97903706 T AT 97903706T AT 97903706 T AT97903706 T AT 97903706T AT E267037 T1 ATE267037 T1 AT E267037T1
Authority
AT
Austria
Prior art keywords
pct
peptides
ion exchange
anion exchanger
high affinity
Prior art date
Application number
AT97903706T
Other languages
German (de)
English (en)
Inventor
Rolf Berglund
Jan Bergstroem
Makonnen Belew
Lennart Soederberg
Original Assignee
Amersham Biosciences Ab
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amersham Biosciences Ab filed Critical Amersham Biosciences Ab
Application granted granted Critical
Publication of ATE267037T1 publication Critical patent/ATE267037T1/de

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
    • B01D15/361—Ion-exchange
    • B01D15/363—Anion-exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J41/00—Anion exchange; Use of material as anion exchangers; Treatment of material for improving the anion exchange properties
    • B01J41/04—Processes using organic exchangers
    • B01J41/07—Processes using organic exchangers in the weakly basic form
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J41/00—Anion exchange; Use of material as anion exchangers; Treatment of material for improving the anion exchange properties
    • B01J41/20—Anion exchangers for chromatographic processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2220/00—Aspects relating to sorbent materials
    • B01J2220/50—Aspects relating to the use of sorbent or filter aid materials
    • B01J2220/54—Sorbents specially adapted for analytical or investigative chromatography

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Peptides Or Proteins (AREA)
AT97903706T 1996-02-19 1997-02-14 Prozess zur chromatographischen trennung von peptiden und nucleinsaure und neue ionentauschermatrix mit hoher affinitat ATE267037T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
SE9600590A SE9600590D0 (sv) 1996-02-19 1996-02-19 Sätt för kromatografisk separation av peptider och nukleinsyra samt ny högaffin jonbytesmatris
PCT/SE1997/000237 WO1997029825A1 (en) 1996-02-19 1997-02-14 Process for chromatographic separation of peptides and nucleic acid, and new high affinity ion exchange matrix

Publications (1)

Publication Number Publication Date
ATE267037T1 true ATE267037T1 (de) 2004-06-15

Family

ID=20401430

Family Applications (1)

Application Number Title Priority Date Filing Date
AT97903706T ATE267037T1 (de) 1996-02-19 1997-02-14 Prozess zur chromatographischen trennung von peptiden und nucleinsaure und neue ionentauschermatrix mit hoher affinitat

Country Status (8)

Country Link
US (1) US6090288A (enExample)
EP (1) EP0888157B1 (enExample)
JP (1) JP4422797B2 (enExample)
AT (1) ATE267037T1 (enExample)
AU (1) AU713635B2 (enExample)
DE (1) DE69729182T2 (enExample)
SE (1) SE9600590D0 (enExample)
WO (1) WO1997029825A1 (enExample)

Families Citing this family (70)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9425138D0 (en) 1994-12-12 1995-02-08 Dynal As Isolation of nucleic acid
KR100463475B1 (ko) 1995-06-08 2005-06-22 로셰 디아그노스틱스 게엠베하 자기성피그먼트
DE19520398B4 (de) 1995-06-08 2009-04-16 Roche Diagnostics Gmbh Magnetisches Pigment
US6027945A (en) 1997-01-21 2000-02-22 Promega Corporation Methods of isolating biological target materials using silica magnetic particles
SE9700769D0 (sv) 1997-03-04 1997-03-04 Pharmacia Biotech Ab Matriser för separation och separation som utnyttjar matriserna
DE19736366A1 (de) * 1997-08-21 1999-02-25 Ernst Prof Dr Bayer Verfahren zur Isolierung von anionischen organischen Verbindungen aus wäßrigen Systemen mit kationischen Polymernanopartikeln
DE69805649T2 (de) * 1997-12-06 2002-12-05 Dna Res Innovations Ltd Isolierung von nukleinsäuren
US6914137B2 (en) 1997-12-06 2005-07-05 Dna Research Innovations Limited Isolation of nucleic acids
US7078224B1 (en) 1999-05-14 2006-07-18 Promega Corporation Cell concentration and lysate clearance using paramagnetic particles
SE9802214D0 (sv) * 1998-06-18 1998-06-18 Amersham Pharm Biotech Ab Jonbytare och användning därav
US6441152B1 (en) * 1998-12-08 2002-08-27 Boston Probes, Inc. Methods, kits and compositions for the identification of nucleic acids electrostatically bound to matrices
EP1147226B1 (en) * 1999-01-27 2013-01-23 Folim G. Halaka Materials and methods for the purification of polyelectrolytes
US6310199B1 (en) 1999-05-14 2001-10-30 Promega Corporation pH dependent ion exchange matrix and method of use in the isolation of nucleic acids
US6270970B1 (en) 1999-05-14 2001-08-07 Promega Corporation Mixed-bed solid phase and its use in the isolation of nucleic acids
SE9904197D0 (sv) 1999-11-22 1999-11-22 Amersham Pharm Biotech Ab A method for anion exchange adsorption on matrices carrying mixed mode ligands
SE9904272D0 (sv) * 1999-11-25 1999-11-25 Amersham Pharm Biotech Ab A method for selective removal of a substance from samples containing compounds having nucleic acid structure
GB9927904D0 (en) * 1999-11-25 2000-01-26 Amersham Pharm Biotech Ab A method fro obtaining a nucleic acid variant
DE60124868T2 (de) * 2000-02-08 2007-06-28 Upfront Chromatography A/S, Kopenhagen Neue harze und ihre verwendung zur rückgewinnung von proteinen oder peptiden
US20030066336A1 (en) * 2000-12-19 2003-04-10 Showa Denko K.K. Method for measuring anions
US20020194901A1 (en) * 2000-12-19 2002-12-26 Showa Denko K.K. Porous polymer particle, alkali-resistant anion exchanger, producing method thereof, column for ion chromatography, and method for measuring anions
SE0004932D0 (sv) * 2000-12-31 2000-12-31 Apbiotech Ab A method for mixed mode adsorption and mixed mode adsorbents
DE60236951D1 (de) * 2001-05-21 2010-08-19 Fujifilm Corp Vorrichtung zur Durchführung biochemischer Analysen und Verfahren zu ihrer Herstellung
SE0103084D0 (sv) * 2001-09-14 2001-09-14 Amersham Pharm Biotech Ab Generation of ion exchange media
GB0127809D0 (en) * 2001-11-20 2002-01-09 Dna Res Innovations Ltd Purification of nucleic acid
CN1617938A (zh) 2002-01-16 2005-05-18 戴诺生物技术有限公司 从单个样品中分离核酸和蛋白质的方法
US20040029143A1 (en) * 2002-02-28 2004-02-12 Jeffrey Van Ness Cationic polyelectrolytes in biomolecule purification and analysis
US20060035224A1 (en) * 2002-03-21 2006-02-16 Johansen Jack T Purification methods for oligonucleotides and their analogs
GB0207975D0 (en) * 2002-04-05 2002-05-15 Genovision As Isolating nucleic acid
GB0212826D0 (en) 2002-05-31 2002-07-10 Dna Res Innovations Ltd Materials and methods relating to polyions and substance delivery
US6989442B2 (en) * 2002-07-12 2006-01-24 Sirna Therapeutics, Inc. Deprotection and purification of oligonucleotides and their derivatives
JP4629660B2 (ja) * 2003-03-05 2011-02-09 ジーイー・ヘルスケア・バイオサイエンス・アクチボラグ マルチモードアニオン交換リガンドの製造法
SE0302911D0 (sv) * 2003-10-31 2003-10-31 Amersham Biosciences Ab Novel separation matrix
US20050106589A1 (en) * 2003-11-17 2005-05-19 Hashem Akhavan-Tafti Compositions and methods for releasing nucleic acids from solid phase binding materials
SE0303532D0 (sv) * 2003-12-23 2003-12-23 Amersham Biosciences Ab Purification of immunoglobulins
US7479222B2 (en) * 2004-02-05 2009-01-20 Millipore Corporation Porous adsorptive or chromatographic media
EP1693108A1 (en) * 2004-12-04 2006-08-23 MERCK PATENT GmbH Mixed-modal anion-exchange type separation material
KR100668337B1 (ko) 2005-05-21 2007-01-12 삼성전자주식회사 단백질에 비하여 핵산에 대한 결합력이 선택적으로 높은ｐＨ 의존성 이온 교환물질, 그가 고정화되어 있는 고체기판, 및 상기 물질 및 고체 기판을 이용하여 핵산을분리하는 방법
WO2007014591A1 (de) 2005-08-03 2007-02-08 Merck Patent Gmbh Hydrophiles vernetztes polymer
EP1754534A1 (de) 2005-08-03 2007-02-21 MERCK PATENT GmbH Hydrophiles vernetztes Polymer
GB2443505B (en) * 2006-09-26 2008-12-31 Ge Healthcare Bio Sciences Nucleic acid purification method
GB2445442A (en) * 2006-09-26 2008-07-09 Ge Healthcare Bio Sciences Nucleic acid purification using anion exchange
GB2445441B (en) * 2006-09-26 2010-06-30 Ge Healthcare Bio Sciences Nucleic acid purification method
DE202008017854U1 (de) 2007-08-14 2010-08-19 Millipore Corp., Billerica Mittel zur Ionenaustausch-Membranchromatographie auf der Basis von primären polymerischen Aminen, Sorptionsvorrichtung mit diesem Mittel, Chromatographieverfahren und Reinigungsverfahren unter dessen Verwendung
US9433922B2 (en) * 2007-08-14 2016-09-06 Emd Millipore Corporation Media for membrane ion exchange chromatography based on polymeric primary amines, sorption device containing that media, and chromatography scheme and purification method using the same
US20090130738A1 (en) * 2007-11-19 2009-05-21 Mikhail Kozlov Media for membrane ion exchange chromatography
WO2009100172A1 (en) 2008-02-07 2009-08-13 Ge Healthcare Bio-Sciences Corp. Isolation of dna, rna and protein from a single sample
WO2009126603A1 (en) 2008-04-08 2009-10-15 Bio-Rad Laboratories, Inc. Chromatography purification of antibodies
WO2009145722A1 (en) * 2008-05-30 2009-12-03 Ge Healthcare Bio-Sciences Ab Separation method utilizing polyallylamine ligands
JP6090985B2 (ja) * 2011-01-12 2017-03-08 積水メディカル株式会社 核酸鎖の分離方法
CN103348242B (zh) 2011-01-12 2016-08-17 积水医疗株式会社 单核苷酸多态性的检测方法
JP5734320B2 (ja) * 2011-02-10 2015-06-17 積水メディカル株式会社 イオン交換クロマトグラフィー用充填剤及び核酸鎖の分離検出方法
US9669402B2 (en) * 2012-03-08 2017-06-06 Bio-Rad Laboratories, Inc. Anionic exchange-hydrophobic mixed mode
US9169304B2 (en) 2012-05-01 2015-10-27 Pfenex Inc. Process for purifying recombinant Plasmodium falciparum circumsporozoite protein
MY178616A (en) 2012-09-17 2020-10-19 Grace W R & Co Chromatography media and devices
ES2730737T3 (es) * 2012-09-17 2019-11-12 Grace W R & Co Material de soporte de partículas funcionalizado y métodos de fabricación y uso del mismo
EP2925432A4 (en) * 2012-11-29 2016-08-31 Affigel Ab SUPER-HYDROXYLATED ADSORBENTS AND USES THEREOF
SG11201605712SA (en) 2014-01-16 2016-08-30 Grace W R & Co Affinity chromatography media and chromatography devices
JP6702587B2 (ja) * 2014-03-14 2020-06-03 ジーイー・ヘルスケア・バイオプロセス・アールアンドディ・アクチボラグ 生物粒子の精製用分離マトリックス
CN107847907A (zh) 2014-05-02 2018-03-27 格雷斯公司 官能化载体材料以及制备和使用官能化载体材料的方法
SG11201609908VA (en) 2014-05-28 2016-12-29 Agency Science Tech & Res Virus reduction method
EP3286172B1 (en) 2015-04-23 2019-06-12 Constellation Pharmaceuticals, Inc. Lsd1 inhibitors and uses thereof
BR112017026193B1 (pt) 2015-06-05 2021-09-14 W.R. Grace & Co-Conn Adsorventes, método de produção dos adsorventes e uso dos adsorventes
WO2018050849A1 (en) * 2016-09-15 2018-03-22 Klawego Gmbh & Co. Kg The use of a polymeric mesh for the purification of macromolecules
LT3532459T (lt) 2016-10-26 2023-11-10 Constellation Pharmaceuticals, Inc. Lsd1 inhibitoriai ir jų medicininis panaudojimas
WO2018235904A1 (ja) * 2017-06-22 2018-12-27 昭和電工株式会社 オリゴヌクレオチドの混合物の分離分析方法
US11666888B2 (en) 2018-02-05 2023-06-06 Bio-Rad Laboratories, Inc. Chromatography resin having an anionic exchange-hydrophobic mixed mode ligand
US12343702B2 (en) 2018-03-08 2025-07-01 Bio-Rad Laboratories, Inc. Anionic exchange-hydrophobic mixed mode chromatography resin
CN114040815B (zh) 2019-09-05 2024-06-28 生物辐射实验室股份有限公司 阴离子交换-疏水混合模式色谱树脂
US11912983B2 (en) 2019-12-05 2024-02-27 Waters Technologies Corporation Polyanionic acids to improve recovery and minimize system loss
EP4182460A1 (en) * 2020-07-14 2023-05-24 Waters Technologies Corporation Kits and methods useful for separating oligonucleotides from matrix components

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4767670A (en) * 1987-01-21 1988-08-30 E. I. Du Pont De Nemours And Company Chromatographic supports for separation of oligonucleotides
SE8902315D0 (sv) * 1989-06-27 1989-06-27 Pharmacia Ab Anjonbytare
US5087359A (en) * 1989-08-07 1992-02-11 J. T. Baker Inc. Quaternized PEI silica solid supports for chromatography
ATE103507T1 (de) * 1989-08-07 1994-04-15 Baker J T Inc Feste traeger aus quaternisierter pei-kieselsaeure fuer die chromatographie.
SE9004129D0 (sv) * 1990-12-21 1990-12-21 Pharmacia Lkb Biotech Anion exchanger

Also Published As

Publication number Publication date
JP2000504625A (ja) 2000-04-18
EP0888157A1 (en) 1999-01-07
SE9600590D0 (sv) 1996-02-19
JP4422797B2 (ja) 2010-02-24
AU713635B2 (en) 1999-12-09
US6090288A (en) 2000-07-18
EP0888157B1 (en) 2004-05-19
DE69729182D1 (de) 2004-06-24
DE69729182T2 (de) 2005-01-27
AU1818097A (en) 1997-09-02
WO1997029825A1 (en) 1997-08-21

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