ATE420186T1 - Verfahren zur reinigung von an mehreren epitopen fusionierten rekombinanten proteinen - Google Patents

Verfahren zur reinigung von an mehreren epitopen fusionierten rekombinanten proteinen

Info

Publication number
ATE420186T1
ATE420186T1 AT00966755T AT00966755T ATE420186T1 AT E420186 T1 ATE420186 T1 AT E420186T1 AT 00966755 T AT00966755 T AT 00966755T AT 00966755 T AT00966755 T AT 00966755T AT E420186 T1 ATE420186 T1 AT E420186T1
Authority
AT
Austria
Prior art keywords
identification
polypeptides
dna
identification polypeptides
purification
Prior art date
Application number
AT00966755T
Other languages
English (en)
Inventor
Bill Brizzard
Ron Hernan
Original Assignee
Sigma Aldrich Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sigma Aldrich Co filed Critical Sigma Aldrich Co
Application granted granted Critical
Publication of ATE420186T1 publication Critical patent/ATE420186T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/04—Linear peptides containing only normal peptide links
    • C07K7/06—Linear peptides containing only normal peptide links having 5 to 11 amino acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/001—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof by chemical synthesis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/04—Linear peptides containing only normal peptide links
    • C07K7/08—Linear peptides containing only normal peptide links having 12 to 20 amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
    • C07K2319/43—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation containing a FLAG-tag

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AT00966755T 1999-10-08 2000-09-20 Verfahren zur reinigung von an mehreren epitopen fusionierten rekombinanten proteinen ATE420186T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US09/415,000 US6379903B1 (en) 1999-10-08 1999-10-08 Purification of recombinant proteins fused to multiple epitopes

Publications (1)

Publication Number Publication Date
ATE420186T1 true ATE420186T1 (de) 2009-01-15

Family

ID=23643935

Family Applications (1)

Application Number Title Priority Date Filing Date
AT00966755T ATE420186T1 (de) 1999-10-08 2000-09-20 Verfahren zur reinigung von an mehreren epitopen fusionierten rekombinanten proteinen

Country Status (9)

Country Link
US (3) US6379903B1 (de)
EP (2) EP1220933B3 (de)
JP (1) JP4405125B2 (de)
AT (1) ATE420186T1 (de)
AU (1) AU774216B2 (de)
CA (1) CA2386471C (de)
DE (1) DE60041345D1 (de)
ES (1) ES2321058T7 (de)
WO (1) WO2001027293A1 (de)

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CA2526120A1 (en) * 2003-06-03 2005-02-24 Cell Genesys, Inc. Compositions and methods for enhanced expression of recombinant polypeptides from a single vector using a peptide cleavage site
US7741053B2 (en) * 2005-05-13 2010-06-22 Sigma-Aldrich Co. Processes for purification of recombinant proteins
US20080039616A1 (en) * 2005-09-29 2008-02-14 Sigma-Aldrich Co. Tandem affinity purification systems and methods utilizing such systems
US7855057B2 (en) * 2006-03-23 2010-12-21 Millipore Corporation Protein splice variant/isoform discrimination and quantitative measurements thereof
WO2008008709A2 (en) * 2006-07-11 2008-01-17 Leap Bioscience Corporation Method of selective protein enrichment and associated applications
US20100062461A1 (en) * 2006-07-11 2010-03-11 Leap Biosciences Corporation Multiplex detection of cell surface receptors or immobilized antigens
CA2954920A1 (en) * 2014-07-14 2016-01-21 The Regents Of The University Of California A protein tagging system for in vivo single molecule imaging and control of gene transcription
WO2019157324A1 (en) 2018-02-08 2019-08-15 Sangamo Therapeutics, Inc. Engineered target specific nucleases
CN112805026A (zh) 2019-02-06 2021-05-14 桑格摩生物治疗股份有限公司 用于治疗i型黏多糖贮积症的方法
BR112021019448A2 (pt) 2019-04-02 2021-11-30 Sangamo Therapeutics Inc Métodos para o tratamento de beta-talassemia
US11492380B2 (en) * 2019-07-24 2022-11-08 Lawrence Livermore National Security, Llc PepCon proteomics standards and methods of use
US20230048224A1 (en) 2019-11-01 2023-02-16 Sangamo Therapeutics, Inc. Zinc finger nuclease variants for treating or preventing lysosomal storage diseases
EP3988659A1 (de) 2020-10-26 2022-04-27 Wageningen Universiteit Prokaryotische post-stop-codon-sequenzen
EP4083204A1 (de) 2021-04-30 2022-11-02 Wageningen Universiteit Genweite randomisierung

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Also Published As

Publication number Publication date
US20020045193A1 (en) 2002-04-18
EP2075336A1 (de) 2009-07-01
CA2386471C (en) 2011-06-21
JP4405125B2 (ja) 2010-01-27
ES2321058T7 (es) 2014-04-07
WO2001027293A1 (en) 2001-04-19
CA2386471A1 (en) 2001-04-19
EP1220933B3 (de) 2013-12-11
US20060088878A1 (en) 2006-04-27
DE60041345D1 (de) 2009-02-26
AU7704900A (en) 2001-04-23
JP2003511078A (ja) 2003-03-25
EP1220933A1 (de) 2002-07-10
US7094548B2 (en) 2006-08-22
AU774216B2 (en) 2004-06-17
EP2075336B1 (de) 2016-01-06
EP1220933B1 (de) 2009-01-07
ES2321058T3 (es) 2009-06-02
US6379903B1 (en) 2002-04-30

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