DE60234131D1 - Neue fusionsproteine und molekulare bindungsassays - Google Patents

Neue fusionsproteine und molekulare bindungsassays

Info

Publication number
DE60234131D1
DE60234131D1 DE60234131T DE60234131T DE60234131D1 DE 60234131 D1 DE60234131 D1 DE 60234131D1 DE 60234131 T DE60234131 T DE 60234131T DE 60234131 T DE60234131 T DE 60234131T DE 60234131 D1 DE60234131 D1 DE 60234131D1
Authority
DE
Germany
Prior art keywords
fusion proteins
expression vectors
binding assays
binding
molecular binding
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60234131T
Other languages
English (en)
Inventor
Gary Bright
Daniel Rajadavid Premkumar
Yih-Tai Chen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cellomics Inc
Original Assignee
Cellomics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cellomics Inc filed Critical Cellomics Inc
Application granted granted Critical
Publication of DE60234131D1 publication Critical patent/DE60234131D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/09—Fusion polypeptide containing a localisation/targetting motif containing a nuclear localisation signal
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/095—Fusion polypeptide containing a localisation/targetting motif containing a nuclear export signal
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
    • C07K2319/41—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation containing a Myc-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/60—Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
DE60234131T 2001-08-01 2002-08-01 Neue fusionsproteine und molekulare bindungsassays Expired - Lifetime DE60234131D1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US30939501P 2001-08-01 2001-08-01
US34158901P 2001-12-13 2001-12-13
PCT/US2002/024572 WO2003012068A2 (en) 2001-08-01 2002-08-01 Novel fusion proteins and assays for molecular binding

Publications (1)

Publication Number Publication Date
DE60234131D1 true DE60234131D1 (de) 2009-12-03

Family

ID=26976793

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60234131T Expired - Lifetime DE60234131D1 (de) 2001-08-01 2002-08-01 Neue fusionsproteine und molekulare bindungsassays

Country Status (8)

Country Link
US (1) US7244614B2 (de)
EP (1) EP1499885B1 (de)
JP (1) JP2005507650A (de)
AT (1) ATE446511T1 (de)
AU (1) AU2002337664B2 (de)
CA (1) CA2453528C (de)
DE (1) DE60234131D1 (de)
WO (1) WO2003012068A2 (de)

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US7167615B1 (en) 1999-11-05 2007-01-23 Board Of Regents, The University Of Texas System Resonant waveguide-grating filters and sensors and methods for making and using same
US8111401B2 (en) 1999-11-05 2012-02-07 Robert Magnusson Guided-mode resonance sensors employing angular, spectral, modal, and polarization diversity for high-precision sensing in compact formats
US7524625B2 (en) * 2000-10-30 2009-04-28 Sru Biosystems, Inc. Real time binding analysis of antigens on a biosensor surface
US7575939B2 (en) 2000-10-30 2009-08-18 Sru Biosystems, Inc. Optical detection of label-free biomolecular interactions using microreplicated plastic sensor elements
US7118710B2 (en) 2000-10-30 2006-10-10 Sru Biosystems, Inc. Label-free high-throughput optical technique for detecting biomolecular interactions
US7371562B2 (en) * 2000-10-30 2008-05-13 Sru Biosystems, Inc. Guided mode resonant filter biosensor using a linear grating surface structure
CA2454238A1 (en) * 2001-07-31 2003-02-13 Clontech Laboratories, Inc. Method of detecting protease activity in a cell
DE60234131D1 (de) * 2001-08-01 2009-12-03 Cellomics Inc Neue fusionsproteine und molekulare bindungsassays
US7927822B2 (en) 2002-09-09 2011-04-19 Sru Biosystems, Inc. Methods for screening cells and antibodies
US7309614B1 (en) 2002-12-04 2007-12-18 Sru Biosystems, Inc. Self-referencing biodetection method and patterned bioassays
JP4374953B2 (ja) * 2003-09-09 2009-12-02 株式会社日立製作所 データ処理システム
US8298780B2 (en) * 2003-09-22 2012-10-30 X-Body, Inc. Methods of detection of changes in cells
US20090131270A1 (en) * 2004-08-02 2009-05-21 Cellumen, Inc.A Corporation Methods for the detection of molecular interactions within cells
DK1988167T3 (da) * 2005-02-17 2020-07-13 Univ Sorbonne Intracellulære inhiberende peptider
US20060275825A1 (en) * 2005-04-12 2006-12-07 Sru Biosystems, Inc. Proteolipid membrane and lipid membrane biosensor
DE602006014519D1 (de) * 2005-12-16 2010-07-08 Nat Cancer Ct Peptide zur transglutaminase-hemmung
GB0526449D0 (en) * 2005-12-23 2006-02-08 Medical Res Council Polypeptide targeting
JP2009526519A (ja) * 2006-01-17 2009-07-23 セルーメン、インコーポレイテッド 生物学的システム応答の予測方法
US8341123B2 (en) 2006-01-27 2012-12-25 El Fresko Technologies Limited Event structured file system (ESFS)
CA2568981A1 (en) * 2006-02-24 2007-08-24 University Of Manitoba Integrase-derived hiv-inhibiting agents
JPWO2007132555A1 (ja) * 2006-05-11 2009-09-24 国立大学法人 東京大学 細胞膜透過性ペプチドと細胞内におけるその使用
CN101484806A (zh) 2006-05-17 2009-07-15 协乐民公司 一种对组织进行自动分析的方法
EP2095119A2 (de) * 2006-11-10 2009-09-02 Cellumen, Inc. Biosensoren für protein-protein-interaktion und verfahren zu ihrer verwendung
WO2008115420A2 (en) * 2007-03-15 2008-09-25 Cellumen, Inc. Methods for detecting molecular interactions within cells using combination of inducible promoters and biosensors
US9134307B2 (en) 2007-07-11 2015-09-15 X-Body, Inc. Method for determining ion channel modulating properties of a test reagent
JP2010532998A (ja) 2007-07-11 2010-10-21 エス アール ユー バイオシステムズ，インコーポレイテッド イオンチャネルのモジュレータを識別する方法
US8257936B2 (en) 2008-04-09 2012-09-04 X-Body Inc. High resolution label free analysis of cellular properties
US20100273185A1 (en) * 2009-04-27 2010-10-28 Sru Biosystems, Inc. Detection of Biased Agonist Activation
US20130266663A1 (en) * 2010-04-30 2013-10-10 Arthur Brown Sox9 inhibitors
ES2739623T3 (es) 2011-03-17 2020-02-03 Cernostics Inc Sistemas y composiciones para diagnosticar el esófago de Barrett y métodos para usarlos
CA2878126C (en) * 2012-07-06 2022-05-10 Innovative Technologies In Biological Systems S.L. Fluorescent fusion polypeptide, biosensor comprising said polypeptide and uses thereof
DK2928491T3 (en) * 2012-12-05 2019-01-14 Thevax Genetics Vaccine Co Ltd FUSION PROTEINS TO USE AS IMMUNOGENEOUS AMPLIFIERS FOR INduction OF ANTIGEN-SPECIFIC T-CELL RESPONSES
EP2997352A4 (de) 2013-03-15 2017-03-01 Bayer Healthcare LLC Substratprofilierung von proteasen in neutrophilen extrazellulären fallen
US10591486B2 (en) 2014-07-23 2020-03-17 Institut National De La Sante Et De La Recherche Medicale (Inserm) Methods and tools for detecting interactions in eukaryotic cells using microtubule structures and dynamics
EP3272864A1 (de) 2016-07-20 2018-01-24 Paul Scherrer Institut Festphasenimmobilisierung von mikrobieller transglutaminase-mtg auf mikrokugeln für proteinkonjugation
AU2018337671B2 (en) * 2017-09-19 2021-03-04 Paul Scherrer Institut Transglutaminase conjugation method and linker
EP4041316A4 (de) * 2019-10-11 2023-10-11 The Board of Trustees of the Leland Stanford Junior University Rekombinante polypeptide zur regulierbaren zellulären lokalisation
WO2024050383A2 (en) * 2022-08-29 2024-03-07 The Regents Of The University Of California Ras biosensors

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US5683888A (en) 1989-07-22 1997-11-04 University Of Wales College Of Medicine Modified bioluminescent proteins and their use
ATE211506T1 (de) 1995-01-31 2002-01-15 Bioimage As Ein verfahren zum nachweis biologisch aktiver substanzen
US5958713A (en) 1995-01-31 1999-09-28 Novo Nordisk A/S Method of detecting biologically active substances by using green fluorescent protein
US5989835A (en) 1997-02-27 1999-11-23 Cellomics, Inc. System for cell-based screening
US5912137A (en) 1996-07-16 1999-06-15 The Regents Of The University Of California Assays for protein kinases using fluorescent
AU3801997A (en) 1996-07-16 1998-02-09 Regents Of The University Of California, The Assays for protein kinases using fluorescent protein substrates
US5925558A (en) 1996-07-16 1999-07-20 The Regents Of The University Of California Assays for protein kinases using fluorescent protein substrates
CA2196496A1 (en) 1997-01-31 1998-07-31 Stephen William Watson Michnick Protein fragment complementation assay for the detection of protein-protein interactions
US6416959B1 (en) 1997-02-27 2002-07-09 Kenneth Giuliano System for cell-based screening
DE69839501D1 (de) 1997-02-27 2008-06-26 Cellomics Inc System für Screening biologischer Zellen
US6727071B1 (en) 1997-02-27 2004-04-27 Cellomics, Inc. System for cell-based screening
US6759206B1 (en) 1997-02-27 2004-07-06 Cellomics, Inc. System for cell-based screening
US6756207B1 (en) 1997-02-27 2004-06-29 Cellomics, Inc. System for cell-based screening
US6197928B1 (en) 1997-03-14 2001-03-06 The Regents Of The University Of California Fluorescent protein sensors for detection of analytes
CA2286293C (en) 1997-04-07 2004-04-06 Bioimage A/S A method for extracting quantitative information relating to an influence on a cellular response
AU4992599A (en) 1998-07-13 2000-02-01 Cellomics, Inc. A system for cell-based screening
EP1121593A2 (de) 1998-10-15 2001-08-08 BioImage A/S Verfahren zur ableitung quantitativer informationen über einen einfluss auf eine zelluläre respons
US6203986B1 (en) * 1998-10-22 2001-03-20 Robert H. Singer Visualization of RNA in living cells
JP3863372B2 (ja) * 1998-10-30 2006-12-27 セロミックス インコーポレイテッド プロテアーゼバイオセンサーをコードする組換え核酸、組換え発現ベクター、プロテアーゼバイオセンサー、化合物を同定する方法、及び化合物を同定するためのキット
GB9901072D0 (en) 1999-01-19 1999-03-10 Imp Cancer Res Tech Methods for detecting changes to a macromolecular component of a cell
WO2000070342A2 (en) 1999-05-14 2000-11-23 Cellomics, Inc. Optical system analysis of cells
US6716588B2 (en) 1999-12-09 2004-04-06 Cellomics, Inc. System for cell-based screening
DE60234131D1 (de) * 2001-08-01 2009-12-03 Cellomics Inc Neue fusionsproteine und molekulare bindungsassays

Also Published As

Publication number Publication date
CA2453528A1 (en) 2003-02-13
WO2003012068A2 (en) 2003-02-13
WO2003012068A3 (en) 2004-11-11
US7244614B2 (en) 2007-07-17
EP1499885B1 (de) 2009-10-21
ATE446511T1 (de) 2009-11-15
AU2002337664B2 (en) 2007-01-25
JP2005507650A (ja) 2005-03-24
EP1499885A2 (de) 2005-01-26
CA2453528C (en) 2011-07-26
EP1499885A4 (de) 2005-11-16
US20030104479A1 (en) 2003-06-05

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