ATE446511T1 - Neue fusionsproteine und molekulare bindungsassays - Google Patents

Neue fusionsproteine und molekulare bindungsassays

Info

Publication number
ATE446511T1
ATE446511T1 AT02773169T AT02773169T ATE446511T1 AT E446511 T1 ATE446511 T1 AT E446511T1 AT 02773169 T AT02773169 T AT 02773169T AT 02773169 T AT02773169 T AT 02773169T AT E446511 T1 ATE446511 T1 AT E446511T1
Authority
AT
Austria
Prior art keywords
fusion proteins
expression vectors
binding assay
binding
molecular binding
Prior art date
Application number
AT02773169T
Other languages
English (en)
Inventor
Gary Bright
Daniel Premkumar
Yih-Tai Chen
Original Assignee
Cellomics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cellomics Inc filed Critical Cellomics Inc
Application granted granted Critical
Publication of ATE446511T1 publication Critical patent/ATE446511T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/09—Fusion polypeptide containing a localisation/targetting motif containing a nuclear localisation signal
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/095—Fusion polypeptide containing a localisation/targetting motif containing a nuclear export signal
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
    • C07K2319/41—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation containing a Myc-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/60—Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
AT02773169T 2001-08-01 2002-08-01 Neue fusionsproteine und molekulare bindungsassays ATE446511T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US30939501P 2001-08-01 2001-08-01
US34158901P 2001-12-13 2001-12-13
PCT/US2002/024572 WO2003012068A2 (en) 2001-08-01 2002-08-01 Novel fusion proteins and assays for molecular binding

Publications (1)

Publication Number Publication Date
ATE446511T1 true ATE446511T1 (de) 2009-11-15

Family

ID=26976793

Family Applications (1)

Application Number Title Priority Date Filing Date
AT02773169T ATE446511T1 (de) 2001-08-01 2002-08-01 Neue fusionsproteine und molekulare bindungsassays

Country Status (8)

Country Link
US (1) US7244614B2 (de)
EP (1) EP1499885B1 (de)
JP (1) JP2005507650A (de)
AT (1) ATE446511T1 (de)
AU (1) AU2002337664B2 (de)
CA (1) CA2453528C (de)
DE (1) DE60234131D1 (de)
WO (1) WO2003012068A2 (de)

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US7575939B2 (en) 2000-10-30 2009-08-18 Sru Biosystems, Inc. Optical detection of label-free biomolecular interactions using microreplicated plastic sensor elements
US7118710B2 (en) 2000-10-30 2006-10-10 Sru Biosystems, Inc. Label-free high-throughput optical technique for detecting biomolecular interactions
US7371562B2 (en) * 2000-10-30 2008-05-13 Sru Biosystems, Inc. Guided mode resonant filter biosensor using a linear grating surface structure
EP1421210A4 (de) * 2001-07-31 2005-02-16 Clontech Lab Inc Verfahren zum nachweis von proteaseaktivität in einer zelle
CA2453528C (en) * 2001-08-01 2011-07-26 Cellomics, Inc. Novel fusion proteins and assays for molecular binding
US7927822B2 (en) 2002-09-09 2011-04-19 Sru Biosystems, Inc. Methods for screening cells and antibodies
US7309614B1 (en) 2002-12-04 2007-12-18 Sru Biosystems, Inc. Self-referencing biodetection method and patterned bioassays
JP4374953B2 (ja) * 2003-09-09 2009-12-02 株式会社日立製作所 データ処理システム
US8298780B2 (en) * 2003-09-22 2012-10-30 X-Body, Inc. Methods of detection of changes in cells
US20090131270A1 (en) * 2004-08-02 2009-05-21 Cellumen, Inc.A Corporation Methods for the detection of molecular interactions within cells
DK1988167T3 (da) * 2005-02-17 2020-07-13 Univ Sorbonne Intracellulære inhiberende peptider
ATE475886T1 (de) * 2005-04-12 2010-08-15 Sru Biosystems Inc Proteolipidmembran und lipidmembranen-biosensor
ATE469168T1 (de) * 2005-12-16 2010-06-15 Nat Cancer Ct Peptide zur transglutaminase-hemmung
GB0526449D0 (en) * 2005-12-23 2006-02-08 Medical Res Council Polypeptide targeting
EP1984737A2 (de) * 2006-01-17 2008-10-29 Cellumen, Inc. Verfahren zur vorhersage von reaktionen biologischer systeme
US8341123B2 (en) 2006-01-27 2012-12-25 El Fresko Technologies Limited Event structured file system (ESFS)
CA2568981A1 (en) * 2006-02-24 2007-08-24 University Of Manitoba Integrase-derived hiv-inhibiting agents
WO2007132555A1 (ja) * 2006-05-11 2007-11-22 The University Of Tokyo 細胞膜透過性ペプチドと細胞内におけるその使用
JP5406019B2 (ja) 2006-05-17 2014-02-05 セルーメン、インコーポレイテッド 自動化組織分析のための方法
EP2095119A2 (de) * 2006-11-10 2009-09-02 Cellumen, Inc. Biosensoren für protein-protein-interaktion und verfahren zu ihrer verwendung
WO2008115420A2 (en) * 2007-03-15 2008-09-25 Cellumen, Inc. Methods for detecting molecular interactions within cells using combination of inducible promoters and biosensors
US9778267B2 (en) 2007-07-11 2017-10-03 X-Body, Inc. Methods for identifying modulators of ion channels
US9134307B2 (en) 2007-07-11 2015-09-15 X-Body, Inc. Method for determining ion channel modulating properties of a test reagent
US8257936B2 (en) 2008-04-09 2012-09-04 X-Body Inc. High resolution label free analysis of cellular properties
US20100273185A1 (en) * 2009-04-27 2010-10-28 Sru Biosystems, Inc. Detection of Biased Agonist Activation
US20130266663A1 (en) * 2010-04-30 2013-10-10 Arthur Brown Sox9 inhibitors
EP3553527A1 (de) 2011-03-17 2019-10-16 Cernostics, Inc. Systeme und zusammensetzungen zur diagnose von barrett-ösophagen und verfahren zur verwendung davon
WO2014006225A1 (en) * 2012-07-06 2014-01-09 Innovative Technologies In Biological Systems S.L. Fluorescent fusion polypeptide, biosensor comprising said polypeptide and uses thereof
CN109553688A (zh) * 2012-12-05 2019-04-02 生控基因疫苗股份有限公司 用作诱发抗原特异性t细胞反应的免疫原性增强剂的融合蛋白
CN105339780A (zh) * 2013-03-15 2016-02-17 拜耳医药保健有限公司 蛋白酶在中性粒细胞胞外诱捕网中的底物性质
US10591486B2 (en) 2014-07-23 2020-03-17 Institut National De La Sante Et De La Recherche Medicale (Inserm) Methods and tools for detecting interactions in eukaryotic cells using microtubule structures and dynamics
EP3272864A1 (de) 2016-07-20 2018-01-24 Paul Scherrer Institut Festphasenimmobilisierung von mikrobieller transglutaminase-mtg auf mikrokugeln für proteinkonjugation
CA3177215A1 (en) * 2017-09-19 2019-03-28 Daiichi Sankyo Company, Limited Antibody-pyrrolobenzodiazepine derivative conjugate
CN114746126A (zh) * 2019-10-11 2022-07-12 小利兰·斯坦福大学理事会 用于可调节细胞定位的重组多肽

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US5683888A (en) 1989-07-22 1997-11-04 University Of Wales College Of Medicine Modified bioluminescent proteins and their use
DK0815257T3 (da) 1995-01-31 2002-04-22 Bioimage As Fremgangsmåde til karakterisering af biologisk aktive stoffer
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CA2453528C (en) * 2001-08-01 2011-07-26 Cellomics, Inc. Novel fusion proteins and assays for molecular binding

Also Published As

Publication number Publication date
US20030104479A1 (en) 2003-06-05
CA2453528A1 (en) 2003-02-13
EP1499885A2 (de) 2005-01-26
AU2002337664B2 (en) 2007-01-25
WO2003012068A3 (en) 2004-11-11
DE60234131D1 (de) 2009-12-03
WO2003012068A2 (en) 2003-02-13
EP1499885B1 (de) 2009-10-21
US7244614B2 (en) 2007-07-17
JP2005507650A (ja) 2005-03-24
CA2453528C (en) 2011-07-26
EP1499885A4 (de) 2005-11-16

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