DK0815257T3 - Fremgangsmåde til karakterisering af biologisk aktive stoffer - Google Patents

Fremgangsmåde til karakterisering af biologisk aktive stoffer

Info

Publication number
DK0815257T3
DK0815257T3 DK96900891T DK96900891T DK0815257T3 DK 0815257 T3 DK0815257 T3 DK 0815257T3 DK 96900891 T DK96900891 T DK 96900891T DK 96900891 T DK96900891 T DK 96900891T DK 0815257 T3 DK0815257 T3 DK 0815257T3
Authority
DK
Denmark
Prior art keywords
biologically active
cell
fluorescence
active substance
gfp
Prior art date
Application number
DK96900891T
Other languages
English (en)
Inventor
Ole Thastrup
Soren Tullin
Lars Kongsbak Poulsen
Sara Petersen Bjorn
Original Assignee
Bioimage As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bioimage As filed Critical Bioimage As
Application granted granted Critical
Publication of DK0815257T3 publication Critical patent/DK0815257T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43595—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from coelenteratae, e.g. medusae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/533—Production of labelled immunochemicals with fluorescent label
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/43504—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from invertebrates
    • G01N2333/43595—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from invertebrates from coelenteratae, e.g. medusae
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/91—Transferases (2.)
    • G01N2333/912—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • G01N2333/91205—Phosphotransferases in general
    • G01N2333/9121—Phosphotransferases in general with an alcohol group as acceptor (2.7.1), e.g. general tyrosine, serine or threonine kinases
DK96900891T 1995-01-31 1996-01-31 Fremgangsmåde til karakterisering af biologisk aktive stoffer DK0815257T3 (da)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DK11095 1995-01-31
DK98295 1995-09-07
PCT/DK1996/000052 WO1996023898A1 (en) 1995-01-31 1996-01-31 A method of detecting biologically active substances

Publications (1)

Publication Number Publication Date
DK0815257T3 true DK0815257T3 (da) 2002-04-22

Family

ID=26063326

Family Applications (1)

Application Number Title Priority Date Filing Date
DK96900891T DK0815257T3 (da) 1995-01-31 1996-01-31 Fremgangsmåde til karakterisering af biologisk aktive stoffer

Country Status (8)

Country Link
EP (1) EP0815257B1 (da)
AT (1) ATE211506T1 (da)
AU (1) AU4483096A (da)
CA (1) CA2217700C (da)
DE (1) DE69618350T2 (da)
DK (1) DK0815257T3 (da)
ES (1) ES2170220T3 (da)
WO (1) WO1996023898A1 (da)

Families Citing this family (64)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5777079A (en) * 1994-11-10 1998-07-07 The Regents Of The University Of California Modified green fluorescent proteins
US5625048A (en) * 1994-11-10 1997-04-29 The Regents Of The University Of California Modified green fluorescent proteins
US6900304B2 (en) 1996-01-31 2005-05-31 The Regents Of The University Of California Emission ratiometric indicators of phosphorylation
US6803188B1 (en) 1996-01-31 2004-10-12 The Regents Of The University Of California Tandem fluorescent protein constructs
US5989835A (en) 1997-02-27 1999-11-23 Cellomics, Inc. System for cell-based screening
AU3801997A (en) * 1996-07-16 1998-02-09 Regents Of The University Of California, The Assays for protein kinases using fluorescent protein substrates
US5912137A (en) * 1996-07-16 1999-06-15 The Regents Of The University Of California Assays for protein kinases using fluorescent
US5925558A (en) * 1996-07-16 1999-07-20 The Regents Of The University Of California Assays for protein kinases using fluorescent protein substrates
US6593135B2 (en) 1996-08-16 2003-07-15 The State Of Oregon, Acting By And Through The State Board Of Higher Education On Behalf Of The University Of Oregon Long wavelength engineered fluorescent proteins
US6124128A (en) 1996-08-16 2000-09-26 The Regents Of The University Of California Long wavelength engineered fluorescent proteins
WO1998013513A2 (en) * 1996-09-24 1998-04-02 Cadus Pharmaceutical Corporation Methods and compositions for identifying receptor effectors
CA2270137A1 (en) * 1996-11-01 1998-05-14 Genespan Corporation Stabilized transient gene expression
US5925449A (en) * 1996-12-26 1999-07-20 Davidovits; Joseph Method for bonding fiber reinforcement on concrete and steel structures and resultant products
JP2001512316A (ja) * 1997-02-13 2001-08-21 メモリアル・スローンケタリング・キャンサー・センター 細胞微環境における変化を光学的に検出する混成分子
US6727071B1 (en) 1997-02-27 2004-04-27 Cellomics, Inc. System for cell-based screening
CA2282658C (en) 1997-02-27 2003-02-25 Cellomics, Inc. A system for cell-based screening
US7117098B1 (en) 1997-02-27 2006-10-03 Cellomics, Inc. Machine-readable storage medium for analyzing distribution of macromolecules between the cell membrane and the cell cytoplasm
US6197928B1 (en) 1997-03-14 2001-03-06 The Regents Of The University Of California Fluorescent protein sensors for detection of analytes
US5998204A (en) * 1997-03-14 1999-12-07 The Regents Of The University Of California Fluorescent protein sensors for detection of analytes
CA2285676C (en) 1997-04-04 2011-03-08 Donald P. Weeks Methods and materials for making and using transgenic dicamba-degrading organisms
DE69824763T2 (de) * 1997-04-07 2005-07-07 Bioimage A/S Verfahren zum Screenen von Substanzen die einen effekt auf intrazelluläre Translokation haben
US6046925A (en) * 1997-04-14 2000-04-04 The Regents Of The University Of California Photochromic fluorescent proteins and optical memory storage devices based on fluorescent proteins
US6376257B1 (en) 1997-04-24 2002-04-23 University Of Rochester Detection by fret changes of ligand binding by GFP fusion proteins
US5891646A (en) 1997-06-05 1999-04-06 Duke University Methods of assaying receptor activity and constructs useful in such methods
GB2328209A (en) * 1997-08-16 1999-02-17 Stefan Andreas Oehler Vectors which express a coloured protein for detection of integration into a cell
US6495664B1 (en) 1998-07-24 2002-12-17 Aurora Biosciences Corporation Fluorescent protein sensors of post-translational modifications
WO2000017221A1 (en) * 1998-09-24 2000-03-30 Duke University Method of measuring protein-protein interactions in living cells
JP2002527761A (ja) * 1998-10-15 2002-08-27 バイオイメージ エイ／エス 細胞応答への影響に関する量的情報を入手するための改良方法
US6410255B1 (en) 1999-05-05 2002-06-25 Aurora Biosciences Corporation Optical probes and assays
EP1055929A1 (en) 1999-05-26 2000-11-29 Tibotec N.V. Means and methods for drug discovery and the phenotypic characterisation of cells
US6573059B1 (en) * 1999-06-04 2003-06-03 Rmf Dictagene S.A. Use of the regulatory subunit of the camp dependent protein kinase (PKA) from dictyostelium for camp measurements
FI107167B (fi) 1999-06-07 2001-06-15 Marko Virta Menetelmä mikro-organismin kasvun ja kuolemisen määrittämiseksi
WO2001009177A2 (en) * 1999-07-29 2001-02-08 Whitehead Institute For Biomedical Research Affinity fluorescent proteins and uses thereof
US6986993B1 (en) 1999-08-05 2006-01-17 Cellomics, Inc. System for cell-based screening
WO2001042786A2 (en) 1999-12-09 2001-06-14 Cellomics, Inc. System for cell based screening : cell spreading
EP1254169B1 (en) 1999-12-30 2007-05-09 President And Fellows of Harvard College Methods relating to modulation of th2 cell subset activity by modulation of xbp-1 activity
JP3829252B2 (ja) 2001-06-08 2006-10-04 独立行政法人理化学研究所 蛍光蛋白質
WO2003012068A2 (en) 2001-08-01 2003-02-13 Cellomics, Inc. Novel fusion proteins and assays for molecular binding
AU2003279273B2 (en) 2002-06-24 2009-10-01 Paratek Pharmaceuticals, Inc. Methods for preventing and treating microbial infections by modulating transcription factors
US20080313769A9 (en) 2006-01-12 2008-12-18 Athenix Corporation EPSP synthase domains conferring glyphosate resistance
WO2007103768A2 (en) 2006-03-02 2007-09-13 Athenix Corporation Methods and compositions for improved enzyme activity in transgenic plant
JP5822466B2 (ja) 2008-03-04 2015-11-24 ワシントン ステイト ユニバーシティー 種子油および膜脂質における脂肪酸不飽和化の示差的調節のための組成物および方法
US9240043B2 (en) 2008-09-16 2016-01-19 Novartis Ag Reproducible quantification of biomarker expression
CN101768213B (zh) 2008-12-30 2012-05-30 中国科学院遗传与发育生物学研究所 一种与植物分蘖数目相关的蛋白及其编码基因与应用
CN101817879A (zh) 2009-02-26 2010-09-01 中国科学院遗传与发育生物学研究所 金属硫蛋白及其编码基因与应用
US8471107B2 (en) 2009-04-16 2013-06-25 Schillinger Genetics, Inc. Soybeans having high germination rates and ultra-low raffinose and stachyose content
WO2011068870A2 (en) 2009-12-01 2011-06-09 President And Fellows Of Harvard College Modulation of nk cell antigen specific effector activity by modulation of cxcr6 (cd186)
WO2012051301A1 (en) 2010-10-12 2012-04-19 President And Fellows Of Harvard College Methods for identifying modulators of triglyceride metabolism, for modulating triglyceride metabolism and for identifying subjects at risk for abnormal triglyceride metabolism
US9956236B2 (en) 2011-02-07 2018-05-01 Cornell University Methods for increasing immune responses using agents that directly bind to and activate IRE-1
ES2931180T3 (es) 2011-05-19 2022-12-27 Fund Publica Andaluza Progreso Y Salud Sistema de tipo Tet-on lentivírico de promotor dual muy inducible
WO2013142571A2 (en) 2012-03-20 2013-09-26 Cornell University Assays for the identification of compounds that modulate lipid homeostasis
WO2014043435A1 (en) 2012-09-14 2014-03-20 Bayer Cropscience Lp Hppd variants and methods of use
DK3049521T3 (da) 2013-09-25 2019-04-23 Univ Cornell Forbindelser til inducering af antitumorimmunitet og fremgangsmåder dertil
EP3117003B1 (en) 2014-03-11 2019-10-30 BASF Agricultural Solutions Seed US LLC Hppd variants and methods of use
WO2015193653A1 (en) 2014-06-16 2015-12-23 Consejo Nacional De Investigaciones Cientificas Y Tecnicas Oxidative resistance chimeric genes and proteins, and transgenic plants including the same
US10597674B2 (en) 2015-09-11 2020-03-24 Basf Agricultural Solutions Seed, Us Llc HPPD variants and methods of use
WO2017184727A1 (en) 2016-04-21 2017-10-26 Bayer Cropscience Lp Tal-effector mediated herbicide tolerance
US11091772B2 (en) 2016-11-23 2021-08-17 BASF Agricultural Solutions Seed US LLC AXMI669 and AXMI991 toxin genes and methods for their use
KR20190095411A (ko) 2016-12-22 2019-08-14 바스프 아그리컬쳐럴 솔루션즈 시드 유에스 엘엘씨 선충 해충의 방제를 위한 ｃｒｙ１４의 용도
UY37571A (es) 2017-01-18 2018-08-31 Bayer Cropscience Lp Gen de toxina bp005 y procedimientos para su uso
US11286498B2 (en) 2017-01-18 2022-03-29 BASF Agricultural Solutions Seed US LLC Use of BP005 for the control of plant pathogens
WO2018165091A1 (en) 2017-03-07 2018-09-13 Bayer Cropscience Lp Hppd variants and methods of use
WO2019083808A1 (en) 2017-10-24 2019-05-02 Basf Se IMPROVING HERBICIDE TOLERANCE AGAINST HPPD INHIBITORS BY REGULATION OF PUTATIVE REDUCED 4-HYDROXYPHENYLPYRUVATE REDUCES IN SOYBEANS
WO2019083810A1 (en) 2017-10-24 2019-05-02 Basf Se IMPROVING HERBICIDE TOLERANCE FOR 4-HYDROXYPHENYLPYRUVATE DIOXYGENASE (HPPD) INHIBITORS BY NEGATIVE REGULATION OF HPPD EXPRESSION IN SOYBEANS

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4220450A (en) * 1978-04-05 1980-09-02 Syva Company Chemically induced fluorescence immunoassay
AU694745B2 (en) * 1993-09-10 1998-07-30 Trustees Of Columbia University In The City Of New York, The Uses of green fluorescent protein
WO1995021191A1 (en) * 1994-02-04 1995-08-10 William Ward Bioluminescent indicator based upon the expression of a gene for a modified green-fluorescent protein

Also Published As

Publication number Publication date
ATE211506T1 (de) 2002-01-15
EP0815257B1 (en) 2002-01-02
CA2217700C (en) 2002-12-17
AU4483096A (en) 1996-08-21
ES2170220T3 (es) 2002-08-01
EP0815257A1 (en) 1998-01-07
WO1996023898A1 (en) 1996-08-08
DE69618350T2 (de) 2002-09-19
DE69618350D1 (de) 2002-02-07
CA2217700A1 (en) 1996-08-08

Similar Documents

Publication Publication Date Title
DK0815257T3 (da) Fremgangsmåde til karakterisering af biologisk aktive stoffer
Haas et al. The immunochemical detection and quantitation of intracellular ubiquitin-protein conjugates.
DE69939896D1 (de) Methoden zur detektion von nukleinsäuren unter verwendung von zymogenen und dazugehörige kits
DK1244808T3 (da) Fremgangsmåde til screening af multipel transformerede celler ved anvendelse af bicistronisk ekspression af fluorescerende proteiner
DE69131181D1 (de) Verfahren zum nachweis und zur quantifikation von zell-subsätzen innerhalb von subpopulationen einer vermischten zellpopulation
AU2849300A (en) Methods and assay kits for detecting mononuclear cell phenotype
ATE283486T1 (de) Differenz-nachweismethoden unter verwendung einer vielzahl von abgestimmten farbstoffen
DE60334144D1 (de) Verfahren und proben zur detektion des tumor spezifische fusion proteinen
DE60010323D1 (de) Fluoreszenzkorrelationsspektroskopie zur identifizierung von verbindung-ziel-bindung
WO2003033650A3 (en) Recombinant renilla reniformis system for bioluminescence resonance energy transfer
Davies et al. Detection of PCNA modifications in Saccharomyces cerevisiae
NZ595791A (en) High throughput assay systems and methods for identifying agents that alter expression of cellular proteins
WO1998029726A3 (en) Method of detecting and determining the concentration of total troponin i in a biological sample
DE69632826D1 (de) Nukleinsaeure erkennung von hepatitis gb virus
DK1143011T3 (da) Fremgangsmåde til påvisning af biologisk aktive substanser
HUP0100948A2 (hu) Antitest kompeticiós teszt (AKT) P-glikoprotein és egyéb sejtfelszíni receptorok, pumpák és fehérjék konformáció-változásainak kvalitatív, illetve konformáció-függő kvantitatív kimutatására és annak alkalmazásai
US7122329B2 (en) Simple quantitative fluorescent assay method for determining the activity of transport proteins of interest
AU2001225377A1 (en) Lipoprotein assay
JP2002335998A (ja) Ｖｅｇｆの生物活性を定量するためのｖｅｇｆ応答性細胞をベースとしたアッセイ
ATE416381T1 (de) Verfahren für den nachweis von prionproteinen
AU2001276569A1 (en) Quantitative fluorescent assay for determining the activity of transport proteins
DK1423708T3 (da) Sammensætninger og fremgangsmåder til bestemmelser af AA4RP
Kajstura et al. Scanning microfluorometric measurement of immunofluorescently labelled microtubules in cultured cells: Dependence of microtubule content on cell density
NZ513455A (en) Method of identifying molecular interactions employing counterselection and at least two hybrid molecules or two hybrid system