ES2170220T3 - Procedimiento para detectar sustancias biologicamente activas. - Google Patents
Procedimiento para detectar sustancias biologicamente activas.Info
- Publication number
- ES2170220T3 ES2170220T3 ES96900891T ES96900891T ES2170220T3 ES 2170220 T3 ES2170220 T3 ES 2170220T3 ES 96900891 T ES96900891 T ES 96900891T ES 96900891 T ES96900891 T ES 96900891T ES 2170220 T3 ES2170220 T3 ES 2170220T3
- Authority
- ES
- Spain
- Prior art keywords
- biologically active
- cell
- fluorescence
- active substance
- gfp
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
- C07K14/43595—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from coelenteratae, e.g. medusae
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
- G01N33/533—Production of labelled immunochemicals with fluorescent label
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
- G01N2333/43504—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from invertebrates
- G01N2333/43595—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from invertebrates from coelenteratae, e.g. medusae
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/91—Transferases (2.)
- G01N2333/912—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- G01N2333/91205—Phosphotransferases in general
- G01N2333/9121—Phosphotransferases in general with an alcohol group as acceptor (2.7.1), e.g. general tyrosine, serine or threonine kinases
Abstract
LA PRESENTE INVENCION SE REFIERE A UN METODO PARA LA DETECCION DE UNA SUBSTANCIA BIOLOGICAMENTE ACTIVA QUE AFECTA A LOS PROCESOS INTRACELULARES, COMPRENDIENDO EL METODO: (A) EL CULTIVO DE UNA CELULA QUE CONTIENE UNA SECUENCIA DE ADN QUE CODIFICA PARA (I) UNA PROTEINA FLUORESCENTE VERDE (GFP) QUE TIENE UN SITIO DE RECONOCIMIENTO PARA LA PROTEINA QUINASA O (II) UNA PROTEINA FLUORESCENTE VERDE EN LA QUE UNO O MAS AMINOACIDOS HAN SIDO SUSTITUIDOS, INSERTADOS O DELECIONADOS PARA OBTENER UN DOMINIO DE UNION DE UN SEGUNDO MENSAJERO O UN SITIO DE RECONOCIMIENTO DE UNA ENZIMA O (III) UN POLIPEPTIDO HIBRIDO DE LA PROTEINA FLUORESCENTE VERDE (GFP) O UNA GFP MODIFICADA Y UN SITIO DE RECONOCIMIENTO PARA UNA ENZIMA O UN DOMINIO DE UNION PARA UN SEGUNDO MENSAJERO EN UNAS CONDICIONES QUE PERMITEN LA EXPRESION DE LA SECUENCIA DE ADN; (B) LA MEDICION DE LA FLUORESCENCIA DE LA CELULA; (C) LA INCUBACION DE LA CELULA CON UNA MUESTRA SOSPECHOSA DE CONTENER UNA SUBSTANCIA BIOLOGICAMENTE ACTIVA QUE AFECTA A LOS PROCESOS INTRACELULARES; Y (D) LA MEDICION DE LA FLUORESCENCIA PRODUCIDA POR LA CELULA INCUBADA Y LA DETERMINACION DE CUALQUIER CAMBIO EN LA FLUORESCENCIA EN COMPARACION CON LA FLUORESCENCIA MEDIDA EN EL PASO (B), SIENDO DICHO CAMBIO INDICATIVO DE LA PRESENCIA DE UNA SUBSTANCIA BIOLOGICAMENTE ACTIVA EN DICHA MUESTRA.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DK11095 | 1995-01-31 | ||
DK98295 | 1995-09-07 |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2170220T3 true ES2170220T3 (es) | 2002-08-01 |
Family
ID=26063326
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES96900891T Expired - Lifetime ES2170220T3 (es) | 1995-01-31 | 1996-01-31 | Procedimiento para detectar sustancias biologicamente activas. |
Country Status (8)
Country | Link |
---|---|
EP (1) | EP0815257B1 (es) |
AT (1) | ATE211506T1 (es) |
AU (1) | AU4483096A (es) |
CA (1) | CA2217700C (es) |
DE (1) | DE69618350T2 (es) |
DK (1) | DK0815257T3 (es) |
ES (1) | ES2170220T3 (es) |
WO (1) | WO1996023898A1 (es) |
Families Citing this family (64)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5625048A (en) | 1994-11-10 | 1997-04-29 | The Regents Of The University Of California | Modified green fluorescent proteins |
US5777079A (en) * | 1994-11-10 | 1998-07-07 | The Regents Of The University Of California | Modified green fluorescent proteins |
US6803188B1 (en) | 1996-01-31 | 2004-10-12 | The Regents Of The University Of California | Tandem fluorescent protein constructs |
US6900304B2 (en) | 1996-01-31 | 2005-05-31 | The Regents Of The University Of California | Emission ratiometric indicators of phosphorylation |
US5989835A (en) | 1997-02-27 | 1999-11-23 | Cellomics, Inc. | System for cell-based screening |
US5925558A (en) * | 1996-07-16 | 1999-07-20 | The Regents Of The University Of California | Assays for protein kinases using fluorescent protein substrates |
US5912137A (en) | 1996-07-16 | 1999-06-15 | The Regents Of The University Of California | Assays for protein kinases using fluorescent |
WO1998002571A1 (en) * | 1996-07-16 | 1998-01-22 | The Regents Of The University Of California | Assays for protein kinases using fluorescent protein substrates |
US6124128A (en) * | 1996-08-16 | 2000-09-26 | The Regents Of The University Of California | Long wavelength engineered fluorescent proteins |
US6593135B2 (en) | 1996-08-16 | 2003-07-15 | The State Of Oregon, Acting By And Through The State Board Of Higher Education On Behalf Of The University Of Oregon | Long wavelength engineered fluorescent proteins |
AU733007B2 (en) * | 1996-09-24 | 2001-05-03 | Cadus Pharmaceutical Corporation | Methods and compositions for identifying receptor effectors |
AU732445B2 (en) * | 1996-11-01 | 2001-04-26 | Genespan Corporation | Stabilized transient gene expression |
US5925449A (en) * | 1996-12-26 | 1999-07-20 | Davidovits; Joseph | Method for bonding fiber reinforcement on concrete and steel structures and resultant products |
US6670449B1 (en) | 1997-02-13 | 2003-12-30 | Memorial Sloan-Kettering Cancer Center | Hybrid molecules and their use for optically detecting changes in cellular microenvironments |
EP0983498B1 (en) | 1997-02-27 | 2004-05-26 | Cellomics, Inc. | A system for cell-based screening |
US6727071B1 (en) | 1997-02-27 | 2004-04-27 | Cellomics, Inc. | System for cell-based screening |
US7117098B1 (en) | 1997-02-27 | 2006-10-03 | Cellomics, Inc. | Machine-readable storage medium for analyzing distribution of macromolecules between the cell membrane and the cell cytoplasm |
US5998204A (en) * | 1997-03-14 | 1999-12-07 | The Regents Of The University Of California | Fluorescent protein sensors for detection of analytes |
US6197928B1 (en) | 1997-03-14 | 2001-03-06 | The Regents Of The University Of California | Fluorescent protein sensors for detection of analytes |
CA2285676C (en) | 1997-04-04 | 2011-03-08 | Donald P. Weeks | Methods and materials for making and using transgenic dicamba-degrading organisms |
JP2001522454A (ja) | 1997-04-07 | 2001-11-13 | バイオイメージ エイ/エス | 細胞応答への影響に関する定量情報の抽出方法 |
US6046925A (en) * | 1997-04-14 | 2000-04-04 | The Regents Of The University Of California | Photochromic fluorescent proteins and optical memory storage devices based on fluorescent proteins |
US6376257B1 (en) | 1997-04-24 | 2002-04-23 | University Of Rochester | Detection by fret changes of ligand binding by GFP fusion proteins |
US5891646A (en) | 1997-06-05 | 1999-04-06 | Duke University | Methods of assaying receptor activity and constructs useful in such methods |
GB2328209A (en) * | 1997-08-16 | 1999-02-17 | Stefan Andreas Oehler | Vectors which express a coloured protein for detection of integration into a cell |
US6495664B1 (en) | 1998-07-24 | 2002-12-17 | Aurora Biosciences Corporation | Fluorescent protein sensors of post-translational modifications |
JP2002526756A (ja) * | 1998-09-24 | 2002-08-20 | デューク ユニバーシティ | 生細胞におけるタンパク質−タンパク質相互作用の測定方法 |
WO2000023615A2 (en) * | 1998-10-15 | 2000-04-27 | Bioimage A/S | Method for extracting quantitative information relating to an influence on a cellular response |
US6410255B1 (en) | 1999-05-05 | 2002-06-25 | Aurora Biosciences Corporation | Optical probes and assays |
EP1055929A1 (en) * | 1999-05-26 | 2000-11-29 | Tibotec N.V. | Means and methods for drug discovery and the phenotypic characterisation of cells |
US6573059B1 (en) * | 1999-06-04 | 2003-06-03 | Rmf Dictagene S.A. | Use of the regulatory subunit of the camp dependent protein kinase (PKA) from dictyostelium for camp measurements |
FI107167B (fi) | 1999-06-07 | 2001-06-15 | Marko Virta | Menetelmä mikro-organismin kasvun ja kuolemisen määrittämiseksi |
WO2001009177A2 (en) * | 1999-07-29 | 2001-02-08 | Whitehead Institute For Biomedical Research | Affinity fluorescent proteins and uses thereof |
US6986993B1 (en) | 1999-08-05 | 2006-01-17 | Cellomics, Inc. | System for cell-based screening |
US6716588B2 (en) | 1999-12-09 | 2004-04-06 | Cellomics, Inc. | System for cell-based screening |
CA2396062C (en) | 1999-12-30 | 2009-11-17 | President And Fellows Of Harvard College | Methods and compositions relating to modulation of hepatocyte growth, plasma cell differentiation or t cell subset activity by modulation of xbp-1 activity |
JP3829252B2 (ja) | 2001-06-08 | 2006-10-04 | 独立行政法人理化学研究所 | 蛍光蛋白質 |
JP2005507650A (ja) | 2001-08-01 | 2005-03-24 | セロミックス インコーポレイテッド | 新規融合タンパク質及び分子結合に関するアッセイ |
CA2489943A1 (en) | 2002-06-24 | 2003-12-31 | Paratek Pharmaceuticals, Inc. | Methods for preventing and treating microbial infections by modulating transcription factors |
US20080313769A9 (en) | 2006-01-12 | 2008-12-18 | Athenix Corporation | EPSP synthase domains conferring glyphosate resistance |
BRPI0708480A2 (pt) | 2006-03-02 | 2011-05-31 | Athenix Corp | processos e composições para aperfeiçoada atividade de enzima em plantas transgênicas |
AU2009221899B2 (en) | 2008-03-04 | 2015-09-03 | Washington State University | Compositions and methods for differential regulation of fatty acid unsaturation in membrane lipids and seed oil |
CA2737116C (en) | 2008-09-16 | 2019-01-15 | Historx, Inc. | Reproducible quantification of biomarker expression |
CN101768213B (zh) | 2008-12-30 | 2012-05-30 | 中国科学院遗传与发育生物学研究所 | 一种与植物分蘖数目相关的蛋白及其编码基因与应用 |
CN101817879A (zh) | 2009-02-26 | 2010-09-01 | 中国科学院遗传与发育生物学研究所 | 金属硫蛋白及其编码基因与应用 |
US8471107B2 (en) | 2009-04-16 | 2013-06-25 | Schillinger Genetics, Inc. | Soybeans having high germination rates and ultra-low raffinose and stachyose content |
WO2011068870A2 (en) | 2009-12-01 | 2011-06-09 | President And Fellows Of Harvard College | Modulation of nk cell antigen specific effector activity by modulation of cxcr6 (cd186) |
WO2012051301A1 (en) | 2010-10-12 | 2012-04-19 | President And Fellows Of Harvard College | Methods for identifying modulators of triglyceride metabolism, for modulating triglyceride metabolism and for identifying subjects at risk for abnormal triglyceride metabolism |
US9956236B2 (en) | 2011-02-07 | 2018-05-01 | Cornell University | Methods for increasing immune responses using agents that directly bind to and activate IRE-1 |
US9822379B2 (en) | 2011-05-19 | 2017-11-21 | Fundación Pública Andaluza Progreso Y Salud | Highly inducible dual-promoter lentiviral TET-ON system |
WO2013142571A2 (en) | 2012-03-20 | 2013-09-26 | Cornell University | Assays for the identification of compounds that modulate lipid homeostasis |
UY35035A (es) | 2012-09-14 | 2014-04-30 | Bayer Cropscience Ag | ?molécula de ácido nucleico recombinante que codifica una enzima hppd, vector, célula huésped, semilla, planta, polipéptido, producto primario, métodos y usos?. |
EP3049521B1 (en) | 2013-09-25 | 2019-03-06 | Cornell University | Compounds for inducing anti-tumor immunity and methods thereof |
CN106459986B (zh) | 2014-03-11 | 2020-06-30 | 拜耳作物科学有限合伙公司 | Hppd变体和使用方法 |
WO2015193653A1 (en) | 2014-06-16 | 2015-12-23 | Consejo Nacional De Investigaciones Cientificas Y Tecnicas | Oxidative resistance chimeric genes and proteins, and transgenic plants including the same |
WO2017042259A1 (en) | 2015-09-11 | 2017-03-16 | Bayer Cropscience Aktiengesellschaft | Hppd variants and methods of use |
WO2017184727A1 (en) | 2016-04-21 | 2017-10-26 | Bayer Cropscience Lp | Tal-effector mediated herbicide tolerance |
BR112019010476A2 (pt) | 2016-11-23 | 2019-09-10 | BASF Agricultural Solutions Seed US LLC | molécula de ácido nucleico recombinante, vetor, célula hospedeira, planta transgênica, semente transgênica, polipeptídeo recombinante, composição, método para controlar uma população de pragas, para matar pragas, para produzir um polipeptídeo, planta ou célula vegetal, método para proteger uma planta contra uma praga, para aumentar o rendimento em uma planta, uso e produto primário |
WO2018119336A1 (en) | 2016-12-22 | 2018-06-28 | Athenix Corp. | Use of cry14 for the control of nematode pests |
UY37570A (es) | 2017-01-18 | 2018-08-31 | Bayer Cropscience Lp | Uso de bp005 para el control de patógenos de planta |
BR112019014727A2 (pt) | 2017-01-18 | 2020-04-07 | BASF Agricultural Solutions Seed US LLC | molécula de ácido nucleico, vector, célula, planta, semente, polipeptídeo, composição, métodos para o controle de uma população de pragas, para matar uma praga, para produzir um polipeptídeo, para proteger uma planta e para aumentar o rendimento em uma planta, uso do ácido nucleico e produto de base |
BR112019018056A2 (pt) | 2017-03-07 | 2020-08-11 | BASF Agricultural Solutions Seed US LLC | molécula de ácido nucleico recombinante, cassete de expressão, célula hospedeira, plantas, sementes transgênicas, polipeptídeo recombinante, métodos para conferir tolerância e para controlar ervas daninhas, produto de utilidade e uso da sequência de nucleotídeos |
US11279944B2 (en) | 2017-10-24 | 2022-03-22 | BASF Agricultural Solutions Seed US LLC | Of herbicide tolerance to 4-hydroxyphenylpyruvate dioxygenase (HPPD) inhibitors by down-regulation of HPPD expression in soybean |
WO2019083808A1 (en) | 2017-10-24 | 2019-05-02 | Basf Se | IMPROVING HERBICIDE TOLERANCE AGAINST HPPD INHIBITORS BY REGULATION OF PUTATIVE REDUCED 4-HYDROXYPHENYLPYRUVATE REDUCES IN SOYBEANS |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4220450A (en) * | 1978-04-05 | 1980-09-02 | Syva Company | Chemically induced fluorescence immunoassay |
US6146826A (en) * | 1993-09-10 | 2000-11-14 | The Trustees Of Columbia University In The City Of New York | Green fluorescent protein |
WO1995021191A1 (en) * | 1994-02-04 | 1995-08-10 | William Ward | Bioluminescent indicator based upon the expression of a gene for a modified green-fluorescent protein |
-
1996
- 1996-01-31 AU AU44830/96A patent/AU4483096A/en not_active Abandoned
- 1996-01-31 AT AT96900891T patent/ATE211506T1/de not_active IP Right Cessation
- 1996-01-31 WO PCT/DK1996/000052 patent/WO1996023898A1/en active IP Right Grant
- 1996-01-31 DK DK96900891T patent/DK0815257T3/da active
- 1996-01-31 CA CA002217700A patent/CA2217700C/en not_active Expired - Lifetime
- 1996-01-31 ES ES96900891T patent/ES2170220T3/es not_active Expired - Lifetime
- 1996-01-31 DE DE69618350T patent/DE69618350T2/de not_active Expired - Lifetime
- 1996-01-31 EP EP96900891A patent/EP0815257B1/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
EP0815257B1 (en) | 2002-01-02 |
DK0815257T3 (da) | 2002-04-22 |
EP0815257A1 (en) | 1998-01-07 |
AU4483096A (en) | 1996-08-21 |
WO1996023898A1 (en) | 1996-08-08 |
CA2217700A1 (en) | 1996-08-08 |
DE69618350D1 (de) | 2002-02-07 |
ATE211506T1 (de) | 2002-01-15 |
CA2217700C (en) | 2002-12-17 |
DE69618350T2 (de) | 2002-09-19 |
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