JP2010532998A - イオンチャネルのモジュレータを識別する方法 - Google Patents
イオンチャネルのモジュレータを識別する方法 Download PDFInfo
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6872—Intracellular protein regulatory factors and their receptors, e.g. including ion channels
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
- G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
Abstract
【選択図】なし
Description
本出願は、本明細書中、その全体が参照として含まれる、2007年7月11日出願の米国シリアル番号第60/949,142号の恩典を主張する。
イオンチャンネルは、薬学及び生命工学業界、特に心臓、肺及び胃腸の健康分野での最も大きな種類の治療標的の一つを構成する。細胞の内外へイオン流動を制御する際に関与するタンパク質の治療的標的化(therapeutic targeting)は患者の健康に劇的な効果がある。イオンチャンネル標的を調節するその能力に関して化合物を試験するのは困難であり、時間の浪費となることがある。パッチクランプアッセイ(patch−clamp assay)は、イオンチャンネルモジュレータの生物学的作用の非常に高感度の測定法である。しかしながらパッチクランプ法は複雑であり、試験化合物の処理量が少ない。
本発明のバイオセンサは比色共鳴反射バイオセンサである。たとえばCunninghamら、“Colorimetric resonant reflection as a direct biochemical assay technique,”、Sensors and Actuators B、第81巻、316−328頁、2002年1月5日;米国特許公開(U.S. Pat. Publ.)第2004/0091397号を参照されたい。比色共鳴バイオセンサは、表面プラズモン共鳴(SPR)バイオセンサではない。SPRバイオセンサは、銀、金、銅、アルミニウム、ナトリウム及びインジウムなどの薄い金属層をもつ。この金属は、好適な波長で光と共鳴し得る伝導帯電子を持たねばならない。光に暴露されたSPRバイオセンサ表面は純粋な金属でなければならない。酸化物、硫化物及び他のフィルムはSPRと干渉する。比色共鳴バイオセンサは金属層を持たない。むしろこれらはTiO2などの高屈折率材料の誘電体コーティングを有する。
たとえば、物理的吸着または化学的結合により、一つ以上の細胞をバイオセンサ上に固定化することができる。細胞は非粘着性細胞でも粘着性細胞であってもよい。細胞は生来、一つ以上のイオンチャンネルまたは一つ以上のチャンネル成分を発現することができる。あるいは細胞は、組み換え的に一つ以上のイオンチャンネルまたはイオンチャンネル成分を発現することができる。細胞は哺乳類細胞、たとえばヒト細胞であり得る。
本発明の一態様では、イオンチャンネルのモジュレータの識別法を提供する。イオンチャンネルのモジュレータは、イオンチャンネルの機能活性に影響を与える。機能活性における影響としては、イオンチャンネル活性の遮断または抑制が挙げられる。遮断または抑制は、イオンチャンネルの刺激剤の存在下、またはこれに応答して起きることができる。あるいはモジュレータは、イオンチャンネルの機能活性を促進または増進することができる。促進は、イオンチャンネルを遮断する分子の存在下で、またはこれに応答して起きることができる。本発明の方法は組み換え細胞と共に使用することができるが、イオンチャンネルタンパク質またはサブユニットを組み換え的に過剰発現する細胞は、本発明の方法で要求されていない。細胞は、比色共鳴反射光バイオセンサ表面の第一の位置に適用することができる。細胞はバイオセンサに固定化できるか、またはこれらは単にバイオセンサ表面上で成長することができる。場合により、細胞は、約1、5、10、30分若しくはそれ以上、または約1、2、5、24、48、36時間若しくはそれ以上の間、本発明の方法の任意の段階でバイオセンサ表面でインキュベートすることができる。アッセイは、約25、30または37℃の温度(または約25〜37℃の間の任意の範囲の温度)で実施することができる。
Claims (12)
- イオンチャネルのアンタゴニストまたはアゴニストを識別する方法であって、
(a)比色共鳴反射光バイオセンサの表面上の第一の位置と第二の位置に細胞を適用すること;
(b)試験試薬を前記第一の位置に適用すること;
(c)前記第二の位置に細胞の既知のイオンチャネルアンタゴニストまたはアゴニストを適用すること;及び
(d)前記第一の位置に関しては比色共鳴反射光第一ピーク波長値(peak wavelength value:PWV)と、前記第二の位置に関しては第二のPWVを検出すること、
を含み、
ここで第一及び第二のPWVが同一または類似であれば、試験試薬はイオンチャネルのアンタゴニストまたはアゴニストであり;第一及び第二のPWVが実質的に異なれば、試験試薬はアンタゴニストでもアゴニストでもない、前記方法。 - 前記第一の位置に細胞を適用後;前記第一の位置に試験試薬を適用後;前記第二の位置に細胞を適用後;既知のイオンチャネルアンタゴニスト若しくはアゴニストを前記第二の位置に適用後;またはこれらの組み合わせの後、前記細胞を一定期間インキュベートする、請求項1に記載の方法。
- 前記PWVは、約2マイクロメートル〜約200マイクロメートルの下限ピクセルサイズをもつレンズを備えたスキャナを使用して検出する、請求項1に記載の方法。
- 前記比色共鳴反射光バイオセンサ表面上の前記第一の位置と第二の位置は、マイクロタイターウェル、マイクロタイタープレート、試験管、ペトリ皿、ミクロフルイドチャネル及びマイクロアレイからなる群から選択される容器の内部表面である、請求項1に記載の方法。
- 前記細胞、試験試薬及びイオンチャネルアンタゴニストまたはアゴニストは、検出標識を含まない、請求項1に記載の方法。
- 約25、30または37℃の温度で前記方法を実施する、請求項1に記載の方法。
- イオンチャネルのモジュレータを識別する方法であって、
(a)比色共鳴反射光バイオセンサの表面上の第一の位置に細胞、試験試薬及び細胞の既知のイオンチャネルアンタゴニストまたはアゴニストを適用すること;
(b)前記第一の位置に関して第一のPWVを検出すること;
(c)比色共鳴反射光バイオセンサ表面上の第二の位置に、細胞と細胞の既知のイオンチャネルアンタゴニストまたはアゴニストを適用すること;及び
(d)前記第二の位置に関して第二のPWVを検出すること;
を、含み、
ここで第一及び第二のPWVが異なっていれば、試験試薬はイオンチャネルのモジュレータであり;第一及び第二のPWVが同一または類似であれば、試験試薬はイオンチャンネルのモジュレータではない、前記方法。 - 前記第一の位置に細胞を適用後;前記第一の位置に試験試薬を適用後;前記第一の位置に既知のイオンチャネルアンタゴニスト若しくはアゴニストを適用後;前記第二の位置に細胞を適用後;前記第二の位置に既知のイオンチャネルアンタゴニストまたはアゴニストを適用後;またはこれらの組み合わせの後、前記細胞を一定期間インキュベートする、請求項7に記載の方法。
- 前記PWVは、約2マイクロメートル〜約200マイクロメートルの下限ピクセルサイズをもつレンズを備えたスキャナを使用して検出する、請求項7に記載の方法。
- 比色共鳴反射光バイオセンサ表面上の前記第一の位置と第二の位置は、マイクロタイターウェル、マイクロタイタープレート、試験管、ペトリ皿、ミクロフルイドチャネル及びマイクロアレイからなる群から選択される容器の内部表面である、請求項7に記載の方法。
- 前記細胞、試験試薬及びイオンチャネルアンタゴニストまたはアゴニストは、検出標識を含まない、請求項7に記載の方法。
- 約25、30または37℃の温度で前記方法を実施する、請求項7に記載の方法。
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US94914207P | 2007-07-11 | 2007-07-11 | |
PCT/US2008/069752 WO2009009718A1 (en) | 2007-07-11 | 2008-07-11 | Methods of identifying modulators of ion channels |
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US (1) | US9778267B2 (ja) |
EP (1) | EP2171454B1 (ja) |
JP (1) | JP2010532998A (ja) |
CN (1) | CN101802611A (ja) |
AU (1) | AU2008274978A1 (ja) |
CA (1) | CA2693700A1 (ja) |
NZ (1) | NZ582473A (ja) |
WO (1) | WO2009009718A1 (ja) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2012526998A (ja) * | 2009-05-15 | 2012-11-01 | エス アール ユー バイオシステムズ,インコーポレイテッド | 細胞集団および混合細胞集団における変化の検出 |
Families Citing this family (14)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7167615B1 (en) | 1999-11-05 | 2007-01-23 | Board Of Regents, The University Of Texas System | Resonant waveguide-grating filters and sensors and methods for making and using same |
US7575939B2 (en) | 2000-10-30 | 2009-08-18 | Sru Biosystems, Inc. | Optical detection of label-free biomolecular interactions using microreplicated plastic sensor elements |
US7524625B2 (en) * | 2000-10-30 | 2009-04-28 | Sru Biosystems, Inc. | Real time binding analysis of antigens on a biosensor surface |
US7927822B2 (en) | 2002-09-09 | 2011-04-19 | Sru Biosystems, Inc. | Methods for screening cells and antibodies |
US7309614B1 (en) | 2002-12-04 | 2007-12-18 | Sru Biosystems, Inc. | Self-referencing biodetection method and patterned bioassays |
US8298780B2 (en) | 2003-09-22 | 2012-10-30 | X-Body, Inc. | Methods of detection of changes in cells |
NZ562794A (en) * | 2005-04-12 | 2010-07-30 | Sru Biosystems Inc | Proteolipid membrane & lipid membrane biosensor using a titanium surface |
US9134307B2 (en) | 2007-07-11 | 2015-09-15 | X-Body, Inc. | Method for determining ion channel modulating properties of a test reagent |
US9778267B2 (en) | 2007-07-11 | 2017-10-03 | X-Body, Inc. | Methods for identifying modulators of ion channels |
US8257936B2 (en) | 2008-04-09 | 2012-09-04 | X-Body Inc. | High resolution label free analysis of cellular properties |
CA2730415A1 (en) * | 2008-07-11 | 2010-01-14 | Sru Biosystems, Inc. | Methods for identifying modulators of ion channels |
US20130330761A1 (en) | 2012-06-12 | 2013-12-12 | Celcuity, LLC | Whole cell assays and methods |
WO2015089380A2 (en) | 2013-12-12 | 2015-06-18 | Celcuity Llc | Assays and methods for determining the responsiveness of an individual subject to a therapeutic agent |
JP7264484B2 (ja) | 2017-03-20 | 2023-04-25 | セルキュイティー インコーポレイテッド | 治療剤選択のためのシグナル伝達経路活性の測定方法 |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20030224369A1 (en) * | 2002-02-25 | 2003-12-04 | Surber Mark W. | Reverse screening and target identification with minicells |
JP2005516210A (ja) * | 2002-01-28 | 2005-06-02 | エス アール ユー バイオシステムス,エル エル シー | 線形光学表面構造を用いた導波モード共鳴フィルターバイオセンサー |
Family Cites Families (216)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3689346A (en) | 1970-09-29 | 1972-09-05 | Rowland Dev Corp | Method for producing retroreflective material |
US3810688A (en) * | 1973-05-21 | 1974-05-14 | A Ballman | Optical waveguiding devices using monocrystalline materials of the sillenite family of bismuth oxides |
US3856404A (en) | 1973-06-06 | 1974-12-24 | Phys Chem Res Corp | Method and apparatus for measuring vapor pressure |
US4009933A (en) * | 1975-05-07 | 1977-03-01 | Rca Corporation | Polarization-selective laser mirror |
US4050895A (en) | 1975-09-26 | 1977-09-27 | Monsanto Research Corporation | Optical analytical device, waveguide and method |
US4668558A (en) * | 1978-07-20 | 1987-05-26 | Minnesota Mining And Manufacturing Company | Shaped plastic articles having replicated microstructure surfaces |
US4576850A (en) * | 1978-07-20 | 1986-03-18 | Minnesota Mining And Manufacturing Company | Shaped plastic articles having replicated microstructure surfaces |
US4344438A (en) | 1978-08-02 | 1982-08-17 | The United States Of America As Represented By The Department Of Health, Education And Welfare | Optical sensor of plasma constituents |
US4240751A (en) | 1978-11-09 | 1980-12-23 | Akzona Incorporated | Method and apparatus for specific binding substances |
US4289371A (en) | 1979-05-31 | 1981-09-15 | Xerox Corporation | Optical scanner using plane linear diffraction gratings on a rotating spinner |
US4321057A (en) | 1979-09-20 | 1982-03-23 | Buckles Richard G | Method for quantitative analysis using optical fibers |
US4420502A (en) | 1980-09-05 | 1983-12-13 | Conley Kenneth E | Apparatus and method for producing a flexible sheet material having a predetermined surface characteristic |
GB2103786A (en) | 1981-08-14 | 1983-02-23 | Ici Plc | Fibre optic sensor |
USRE33064E (en) | 1981-09-18 | 1989-09-19 | Prutec Limited | Method for the determination of species in solution with an optical wave-guide |
DE3277030D1 (en) | 1981-09-18 | 1987-09-24 | Battelle Memorial Institute | Method and apparatus for the determination of species in solution with an optical wave-guide |
DE3376685D1 (en) * | 1982-12-21 | 1988-06-23 | Ares Serono Nv | Assay technique |
US4536608A (en) | 1983-04-25 | 1985-08-20 | Exxon Research And Engineering Co. | Solar cell with two-dimensional hexagonal reflecting diffraction grating |
US4652290A (en) * | 1983-07-05 | 1987-03-24 | Motorola, Inc. | Method for making optical channel waveguides and product manufactured thereby |
GB2156970B (en) | 1984-01-06 | 1987-09-16 | Plessey Co Plc | Optical detection of specific molecules |
WO1986000135A1 (en) * | 1984-06-13 | 1986-01-03 | Unilever Plc | Photometric instruments, their use in methods of optical analyses, and ancillary devices therefor |
US4701008A (en) | 1984-08-10 | 1987-10-20 | Motorola, Inc. | Optical waveguide including superstrate of niobium or silicon oxynitride and method of making same |
GB8423204D0 (en) | 1984-09-14 | 1984-10-17 | Comtech Res Unit | Assay technique and equipment |
US4650329A (en) * | 1984-11-29 | 1987-03-17 | The United States Of America As Represented By The Secretary Of The Navy | Optical 3-d signature device for detecting chemical agents |
EP0184600B1 (en) * | 1984-12-10 | 1990-03-14 | Prutec Limited | Method for optically ascertaining parameters of species in a liquid analyte |
GB8509491D0 (en) | 1985-04-12 | 1985-05-15 | Plessey Co Plc | Optic waveguide biosensors |
AU5815886A (en) | 1985-05-29 | 1986-12-24 | Kurt Tiefenthaler | Optical sensor for selectively determining the presence of substances and the variation of the refraction index in the measured substances |
CH669050A5 (de) | 1985-05-29 | 1989-02-15 | Oerlikon Buehrle Holding Ag | Sensor zum nachweis von aenderungen der brechzahl einer festen oder fluessigen messsubstanz. |
CH670521A5 (en) | 1985-05-29 | 1989-06-15 | Oerlikon Buehrle Holding Ag | Optical sensor detecting specific substances in material |
GB8618133D0 (en) * | 1986-07-24 | 1986-09-03 | Pa Consulting Services | Biosensors |
US4876208A (en) | 1987-01-30 | 1989-10-24 | Yellowstone Diagnostics Corporation | Diffraction immunoassay apparatus and method |
GB8705649D0 (en) * | 1987-03-10 | 1987-04-15 | Pa Consulting Services | Assay sensor |
US4888260A (en) | 1987-08-10 | 1989-12-19 | Polaroid Corporation | Volume phase reflection holograms and methods for fabricating them |
US4999234A (en) * | 1987-08-10 | 1991-03-12 | Polaroid Corporation | Holographic optical data storage medium |
NL8800219A (nl) | 1988-01-29 | 1989-08-16 | Philips Nv | Roteerbare magneetkopeenheid voor een magneetbandapparaat. |
US4952056A (en) | 1988-05-17 | 1990-08-28 | Entwicklungsgemeinschaft Asi | Method of determining the autocollimation angle of a grating coupler |
GB2220080A (en) | 1988-06-24 | 1989-12-28 | Marconi Gec Ltd | Improvements in optical waveguides |
US6235488B1 (en) * | 1988-09-29 | 2001-05-22 | Agilent Technologies, Inc. | Surface preparation for chemical-specific binding |
SE462454B (sv) | 1988-11-10 | 1990-06-25 | Pharmacia Ab | Maetyta foer anvaendning i biosensorer |
SE8804074D0 (sv) * | 1988-11-10 | 1988-11-10 | Pharmacia Ab | Sensorenhet och dess anvaendning i biosensorsystem |
GB2227089A (en) | 1989-01-11 | 1990-07-18 | Plessey Co Plc | An optical biosensor |
US5252293A (en) | 1989-01-17 | 1993-10-12 | Vladimir Drbal | Analytical slide with porous filter membrane |
US5175030A (en) | 1989-02-10 | 1992-12-29 | Minnesota Mining And Manufacturing Company | Microstructure-bearing composite plastic articles and method of making |
GB8916764D0 (en) * | 1989-07-21 | 1989-09-06 | Sambles John R | Surface plasmon optical sensor |
US5156785A (en) | 1991-07-10 | 1992-10-20 | Cordis Corporation | Extruded tubing and catheters having increased rotational stiffness |
US5541057A (en) * | 1989-09-18 | 1996-07-30 | Biostar, Inc. | Methods for detection of an analyte |
WO1991013339A1 (en) | 1990-03-02 | 1991-09-05 | Fisons Plc | Sample cell for use in chemical or biochemical assays |
EP0455067B1 (de) | 1990-05-03 | 2003-02-26 | F. Hoffmann-La Roche Ag | Mikrooptischer Sensor |
IL98150A0 (en) | 1990-05-17 | 1992-08-18 | Adeza Biomedical Corp | Highly reflective biogratings and method for theirhighly reflective biogratings and method production |
US5478756A (en) | 1990-07-24 | 1995-12-26 | Fisons Plc | Chemical sensor for detecting binding reactions |
CA2088372C (en) | 1990-08-31 | 1999-10-05 | Jayantilal Shamjibhai Patel | Tunable liquid-crystal etalon filter |
US5337183A (en) | 1991-02-01 | 1994-08-09 | Yeda Research And Development Co. Ltd. | Distributed resonant cavity light beam modulator |
SE468188B (sv) * | 1991-04-08 | 1992-11-16 | Stiftelsen Inst Foer Mikroelek | Metod foer inkoppling av straalning i en infraroeddetektor, jaemte anordning |
WO1992019976A1 (de) | 1991-04-26 | 1992-11-12 | Gesellschaft Zur Förderung Der Industrieorientierten Forschung An Den Schweizerischen Hochschulen Und Weiteren Institutionen | Verfahren und vorrichtung zur bestimmung einer messgrösse mittels eines integriert-optischen sensormoduls |
US5155785A (en) | 1991-05-01 | 1992-10-13 | At&T Bell Laboratories | Optical fiber interconnection apparatus and method |
GB9111912D0 (en) | 1991-06-04 | 1991-07-24 | Fisons Plc | Analytical methods |
GB2256477B (en) * | 1991-06-07 | 1995-03-08 | Marconi Gec Ltd | An optical sensor |
US5216680A (en) | 1991-07-11 | 1993-06-01 | Board Of Regents, The University Of Texas System | Optical guided-mode resonance filter |
US5170448A (en) | 1992-01-06 | 1992-12-08 | Motorola, Inc. | Optical waveguide apparatus and method for partially collecting light |
GB9200562D0 (en) | 1992-01-11 | 1992-03-11 | Fisons Plc | Analytical device with polychromatic light source |
US5268782A (en) | 1992-01-16 | 1993-12-07 | Minnesota Mining And Manufacturing Company | Micro-ridged, polymeric liquid crystal display substrate and display device |
JPH05228946A (ja) | 1992-02-21 | 1993-09-07 | Kuraray Co Ltd | 光学部品の製造法および光学部品複製用母型 |
US5494829A (en) * | 1992-07-31 | 1996-02-27 | Biostar, Inc. | Devices and methods for detection of an analyte based upon light interference |
US5413884A (en) * | 1992-12-14 | 1995-05-09 | American Telephone And Telegraph Company | Grating fabrication using electron beam lithography |
US5615052A (en) * | 1993-04-16 | 1997-03-25 | Bruce W. McCaul | Laser diode/lens assembly |
US5512492A (en) * | 1993-05-18 | 1996-04-30 | University Of Utah Research Foundation | Waveguide immunosensor with coating chemistry providing enhanced sensitivity |
EP0702610B1 (en) | 1993-06-11 | 1997-05-28 | Minnesota Mining And Manufacturing Company | Laser machined replication tooling |
US5475780A (en) | 1993-06-17 | 1995-12-12 | At&T Corp. | Optical waveguiding component comprising a band-pass filter |
GB9314991D0 (en) | 1993-07-20 | 1993-09-01 | Sandoz Ltd | Mechanical device |
AU679968B2 (en) * | 1993-09-13 | 1997-07-17 | Minnesota Mining And Manufacturing Company | Abrasive article, method of manufacture of same, method of using same for finishing, and a production tool |
US6042998A (en) * | 1993-09-30 | 2000-03-28 | The University Of New Mexico | Method and apparatus for extending spatial frequencies in photolithography images |
US5691846A (en) | 1993-10-20 | 1997-11-25 | Minnesota Mining And Manufacturing Company | Ultra-flexible retroreflective cube corner composite sheetings and methods of manufacture |
US5478848A (en) * | 1994-01-26 | 1995-12-26 | Bayer Corporation | Inhibition of arthritis by L-type calcium channel antagonists nimodipine, nisoldipine and nifedipine |
US5559338A (en) | 1994-10-04 | 1996-09-24 | Excimer Laser Systems, Inc. | Deep ultraviolet optical imaging system for microlithography and/or microfabrication |
US5955335A (en) | 1994-10-08 | 1999-09-21 | Foschungszentrum Julich GmbH | Biomaterial immobilization on an Si3 N4 surface containing Si-NH2 groups with a heterobifunctional cross-linking agent |
TW323341B (ja) * | 1995-01-09 | 1997-12-21 | Minnesota Mining & Mfg | |
US5606170A (en) * | 1995-02-03 | 1997-02-25 | Research International, Inc. | Multifunctional sensor system |
US5601997A (en) | 1995-02-03 | 1997-02-11 | Tchao; Ruy | Chemotaxis assay procedure |
WO1996027757A1 (en) * | 1995-03-03 | 1996-09-12 | Minnesota Mining And Manufacturing Company | Light directing film having variable height structured surface and light directing article constructed therefrom |
US5690894A (en) | 1995-05-23 | 1997-11-25 | The Regents Of The University Of California | High density array fabrication and readout method for a fiber optic biosensor |
WO1996038726A1 (en) | 1995-05-30 | 1996-12-05 | Ecole Polytechnique Federale De Lausanne (Epfl) | Covalently immobilized phospholipid bilayers on solid surfaces |
US5598300A (en) * | 1995-06-05 | 1997-01-28 | Board Of Regents, The University Of Texas System | Efficient bandpass reflection and transmission filters with low sidebands based on guided-mode resonance effects |
US6200737B1 (en) * | 1995-08-24 | 2001-03-13 | Trustees Of Tufts College | Photodeposition method for fabricating a three-dimensional, patterned polymer microstructure |
EP0795121B1 (de) | 1995-09-01 | 2003-11-12 | Paul Scherrer Institut | Verfahren und vorrichtung zur messung von lichtbündeln |
GB9518429D0 (en) | 1995-09-08 | 1995-11-08 | Pharmacia Biosensor | A rapid method for providing kinetic and structural data in molecular interaction analysis |
US6174677B1 (en) * | 1995-10-13 | 2001-01-16 | Ut-Battelle, Llc | Advanced surface-enhanced Raman gene probe systems and methods thereof |
US5814516A (en) | 1995-10-13 | 1998-09-29 | Lockheed Martin Energy Systems, Inc. | Surface enhanced Raman gene probe and methods thereof |
US5822486A (en) * | 1995-11-02 | 1998-10-13 | General Scanning, Inc. | Scanned remote imaging method and system and method of determining optimum design characteristics of a filter for use therein |
US5814524A (en) | 1995-12-14 | 1998-09-29 | Trustees Of Tufts College | Optical sensor apparatus for far-field viewing and making optical analytical measurements at remote locations |
GB9602542D0 (en) | 1996-02-08 | 1996-04-10 | Fisons Plc | Analytical device |
US5804453A (en) | 1996-02-09 | 1998-09-08 | Duan-Jun Chen | Fiber optic direct-sensing bioprobe using a phase-tracking approach |
US5801390A (en) | 1996-02-09 | 1998-09-01 | Nikon Corporation | Position-detection method and apparatus with a grating mark |
JP2000508083A (ja) * | 1996-03-27 | 2000-06-27 | ブリティッシュ・テレコミュニケーションズ・パブリック・リミテッド・カンパニー | 光回折格子 |
US5821343A (en) | 1996-04-25 | 1998-10-13 | Medtronic Inc | Oxidative method for attachment of biomolecules to surfaces of medical devices |
IL118209A0 (en) * | 1996-05-09 | 1998-02-08 | Yeda Res & Dev | Active electro-optical wavelength-selective mirrors and active electro-optic wavelength-selective filters |
US6174497B1 (en) * | 1997-06-04 | 2001-01-16 | Euro-Celtique, S.A. | Detection systems and methods for predicting the dissolution curve of a drug from a pharmaceutical dosage form |
KR20000016498A (ko) * | 1996-06-10 | 2000-03-25 | 다니엘 제이. 설리반 | 생산환경을 위한 홀로그래픽 패터닝방법 및 공구 |
US5666197A (en) | 1996-08-21 | 1997-09-09 | Polaroid Corporation | Apparatus and methods employing phase control and analysis of evanescent illumination for imaging and metrology of subwavelength lateral surface topography |
AU4207897A (en) * | 1996-08-29 | 1998-03-19 | Novartis Ag | Optical chemical / biochemical sensor |
GB9618635D0 (en) | 1996-09-06 | 1996-10-16 | Thermo Fast Uk Ltd | Improvements in or relating to sensors |
US5846843A (en) | 1996-11-18 | 1998-12-08 | The University Of Toledo | Sensor using long range surface plasmon resonance with diffraction double-grating |
US5922550A (en) * | 1996-12-18 | 1999-07-13 | Kimberly-Clark Worldwide, Inc. | Biosensing devices which produce diffraction images |
WO1998030679A1 (en) | 1997-01-10 | 1998-07-16 | Life Technologies, Inc. | Embryonic stem cell serum replacement |
US5864641A (en) * | 1997-04-11 | 1999-01-26 | F&S, Inc. | Optical fiber long period sensor having a reactive coating |
US6035089A (en) * | 1997-06-11 | 2000-03-07 | Lockheed Martin Energy Research Corporation | Integrated narrowband optical filter based on embedded subwavelength resonant grating structures |
US5925878A (en) | 1997-08-20 | 1999-07-20 | Imation Corp. | Diffraction anomaly sensor having grating coated with protective dielectric layer |
US5955378A (en) | 1997-08-20 | 1999-09-21 | Challener; William A. | Near normal incidence optical assaying method and system having wavelength and angle sensitivity |
US6902703B2 (en) * | 1999-05-03 | 2005-06-07 | Ljl Biosystems, Inc. | Integrated sample-processing system |
US6128431A (en) | 1997-10-08 | 2000-10-03 | The Regents Of The University Of California | High efficiency source coupler for optical waveguide illumination system |
US5994150A (en) | 1997-11-19 | 1999-11-30 | Imation Corp. | Optical assaying method and system having rotatable sensor disk with multiple sensing regions |
US6338968B1 (en) * | 1998-02-02 | 2002-01-15 | Signature Bioscience, Inc. | Method and apparatus for detecting molecular binding events |
US6210910B1 (en) * | 1998-03-02 | 2001-04-03 | Trustees Of Tufts College | Optical fiber biosensor array comprising cell populations confined to microcavities |
DE19814811C1 (de) * | 1998-04-02 | 1999-08-05 | Inst Physikalische Hochtech Ev | Anordnung für die Oberflächenplasmonen-Resonanz-Spektroskopie |
NL1008934C2 (nl) | 1998-04-20 | 1999-10-21 | Univ Twente | Geïntegreerde optische lichtgeleider inrichting. |
EP1084454B1 (en) | 1998-04-21 | 2016-03-09 | University of Connecticut | Free-form nanofabrication using multi-photon excitation |
US5998298A (en) | 1998-04-28 | 1999-12-07 | Sandia Corporation | Use of chemical-mechanical polishing for fabricating photonic bandgap structures |
TW460758B (en) * | 1998-05-14 | 2001-10-21 | Holographic Lithography System | A holographic lithography system for generating an interference pattern suitable for selectively exposing a photosensitive material |
US6346376B1 (en) * | 1998-06-03 | 2002-02-12 | Centre Suisse D'electronique Et De Mictotechnique Sa | Optical sensor unit and procedure for the ultrasensitive detection of chemical or biochemical analytes |
US5986762A (en) | 1998-06-15 | 1999-11-16 | Imation Corp. | Optical sensor having optimized surface profile |
US6406921B1 (en) * | 1998-07-14 | 2002-06-18 | Zyomyx, Incorporated | Protein arrays for high-throughput screening |
US6684007B2 (en) | 1998-10-09 | 2004-01-27 | Fujitsu Limited | Optical coupling structures and the fabrication processes |
US6320991B1 (en) * | 1998-10-16 | 2001-11-20 | Imation Corp. | Optical sensor having dielectric film stack |
US6092703A (en) * | 1998-11-05 | 2000-07-25 | Johnson; Andrew L. | Holster having a frontal reinforcement |
WO2000029830A1 (en) | 1998-11-13 | 2000-05-25 | Leica Microsystems, Inc. | Refractometer and method for qualitative and quantitative measurements |
US6579673B2 (en) * | 1998-12-17 | 2003-06-17 | Kimberly-Clark Worldwide, Inc. | Patterned deposition of antibody binding protein for optical diffraction-based biosensors |
US6303179B1 (en) | 1999-02-08 | 2001-10-16 | Medtronic, Inc | Method for attachment of biomolecules to surfaces through amine-functional groups |
EP1031828B1 (en) | 1999-02-25 | 2006-09-13 | C.S.E.M. Centre Suisse D'electronique Et De Microtechnique Sa | Integrated-optical sensor and method for integrated-optically sensing a substance |
US6332663B1 (en) | 1999-06-16 | 2001-12-25 | Xerox Corporation | Methods and apparatus for marking images and obtaining image data using a single marking engine platform |
AU5824300A (en) | 1999-07-05 | 2001-01-22 | Novartis Ag | Sensor platform, apparatus incorporating the platform, and process using the platform |
US6771376B2 (en) * | 1999-07-05 | 2004-08-03 | Novartis Ag | Sensor platform, apparatus incorporating the platform, and process using the platform |
CA2378180A1 (en) | 1999-07-12 | 2001-01-18 | Aztec Systems, Inc. | Electro-optic device allowing wavelength tuning |
DE69909480T2 (de) | 1999-09-15 | 2004-04-15 | Csem Centre Suisse D'electronique Et De Microtechnique S.A. | Integriert-optischer Sensor |
US6376177B1 (en) | 1999-10-06 | 2002-04-23 | Virtual Pro, Inc. | Apparatus and method for the analysis of nucleic acids hybridization on high density NA chips |
US20050074825A1 (en) | 1999-10-25 | 2005-04-07 | Ying Luo | Tankyrase H, compositions involved in the cell cycle and methods of use |
KR100390875B1 (ko) * | 1999-10-27 | 2003-07-10 | (주)해빛정보 | 위상 회절 격자형 광 저대역 통과필터 |
US7167615B1 (en) * | 1999-11-05 | 2007-01-23 | Board Of Regents, The University Of Texas System | Resonant waveguide-grating filters and sensors and methods for making and using same |
FR2801977B1 (fr) | 1999-12-02 | 2002-05-17 | Commissariat Energie Atomique | Amplification d'un signal de fluorescence emis par un echantillon surfacique |
ATE469350T1 (de) | 2000-02-16 | 2010-06-15 | Wisconsin Alumni Res Found | Biochemische blockierungsschicht für flüssigkristall |
WO2001061357A2 (en) | 2000-02-16 | 2001-08-23 | Wisconsin Alumni Research Foundation | Method and apparatus for detection of microscopic pathogens |
US6490094B2 (en) * | 2000-03-17 | 2002-12-03 | Zograph, Llc | High acuity lens system |
US6639674B2 (en) | 2000-03-28 | 2003-10-28 | Board Of Regents, The University Of Texas System | Methods and apparatus for polarized reflectance spectroscopy |
AU2001257074A1 (en) | 2000-04-18 | 2001-10-30 | Wayne State University | System to detect protein-protein interactions |
JP4812223B2 (ja) * | 2000-06-02 | 2011-11-09 | バイエル・テクノロジー・サービシーズ・ゲゼルシャフト・ミット・ベシュレンクテル・ハフツング | 多分析対象物測定のためのキット及び方法 |
US6449097B1 (en) | 2000-06-05 | 2002-09-10 | Lightchip, Inc. | Diffraction grating for wavelength division multiplexing/demultiplexing devices |
US7399599B2 (en) | 2000-07-10 | 2008-07-15 | Vertex Pharmaceuticals (San Diego) Llc | Ion channel assay methods |
US20040219619A1 (en) | 2000-07-21 | 2004-11-04 | Ester Fernandez-Salas | Methods of identifying compounds that alter toxin persistence and/or protease activity |
WO2002016917A1 (en) * | 2000-08-18 | 2002-02-28 | Lattice Intellectual Property Ltd | Method and apparatus for detecting chemical contamination |
US7142296B2 (en) * | 2000-10-30 | 2006-11-28 | Sru Biosystems, Inc. | Method and apparatus for detecting biomolecular interactions |
US20030092075A1 (en) * | 2000-10-30 | 2003-05-15 | Sru Biosystems, Llc | Aldehyde chemical surface activation processes and test methods for colorimetric resonant sensors |
US7023544B2 (en) * | 2000-10-30 | 2006-04-04 | Sru Biosystems, Inc. | Method and instrument for detecting biomolecular interactions |
US7524625B2 (en) | 2000-10-30 | 2009-04-28 | Sru Biosystems, Inc. | Real time binding analysis of antigens on a biosensor surface |
US7202076B2 (en) * | 2000-10-30 | 2007-04-10 | Sru Biosystems, Inc. | Label-free high-throughput optical technique for detecting biomolecular interactions |
US7306827B2 (en) * | 2000-10-30 | 2007-12-11 | Sru Biosystems, Inc. | Method and machine for replicating holographic gratings on a substrate |
US7264973B2 (en) * | 2000-10-30 | 2007-09-04 | Sru Biosystems, Inc. | Label-free methods for performing assays using a colorimetric resonant optical biosensor |
US7118710B2 (en) | 2000-10-30 | 2006-10-10 | Sru Biosystems, Inc. | Label-free high-throughput optical technique for detecting biomolecular interactions |
US7575939B2 (en) * | 2000-10-30 | 2009-08-18 | Sru Biosystems, Inc. | Optical detection of label-free biomolecular interactions using microreplicated plastic sensor elements |
US7371562B2 (en) | 2000-10-30 | 2008-05-13 | Sru Biosystems, Inc. | Guided mode resonant filter biosensor using a linear grating surface structure |
US7217574B2 (en) * | 2000-10-30 | 2007-05-15 | Sru Biosystems, Inc. | Method and apparatus for biosensor spectral shift detection |
US7175980B2 (en) * | 2000-10-30 | 2007-02-13 | Sru Biosystems, Inc. | Method of making a plastic colorimetric resonant biosensor device with liquid handling capabilities |
US20030113766A1 (en) * | 2000-10-30 | 2003-06-19 | Sru Biosystems, Llc | Amine activated colorimetric resonant biosensor |
US7101660B2 (en) * | 2000-10-30 | 2006-09-05 | Sru Biosystems, Inc. | Method for producing a colorimetric resonant reflection biosensor on rigid surfaces |
US6951715B2 (en) * | 2000-10-30 | 2005-10-04 | Sru Biosystems, Inc. | Optical detection of label-free biomolecular interactions using microreplicated plastic sensor elements |
US7615339B2 (en) | 2000-10-30 | 2009-11-10 | Sru Biosystems, Inc. | Method for producing a colorimetric resonant reflection biosensor on rigid surfaces |
US7300803B2 (en) * | 2000-10-30 | 2007-11-27 | Sru Biosystems, Inc. | Label-free methods for performing assays using a colorimetric resonant reflectance optical biosensor |
US7153702B2 (en) * | 2000-10-30 | 2006-12-26 | Sru Biosystems, Inc. | Label-free methods for performing assays using a colorimetric resonant reflectance optical biosensor |
US6870624B2 (en) * | 2000-10-30 | 2005-03-22 | Coho Holdings Llc | Optical wavelength resonant device for chemical sensing |
US7033821B2 (en) | 2000-11-08 | 2006-04-25 | Surface Logix, Inc. | Device for monitoring cell motility in real-time |
US7033819B2 (en) | 2000-11-08 | 2006-04-25 | Surface Logix, Inc. | System for monitoring cell motility in real-time |
US6893836B2 (en) | 2000-11-29 | 2005-05-17 | Picoliter Inc. | Spatially directed ejection of cells from a carrier fluid |
WO2002045125A1 (en) | 2000-12-01 | 2002-06-06 | Yeda Research And Development Co. Ltd. | Device and method for the examination of samples in a non-vacuum environment using a scanning electron microscope |
US6661952B2 (en) | 2001-05-04 | 2003-12-09 | Ut Battelle, Llc | Sub-wavelength efficient polarization filter (SWEP filter) |
ATE446511T1 (de) | 2001-08-01 | 2009-11-15 | Cellomics Inc | Neue fusionsproteine und molekulare bindungsassays |
US6748138B2 (en) * | 2001-09-14 | 2004-06-08 | Fibera, Inc. | Optical grating fabrication |
AU2002367539A1 (en) * | 2001-11-07 | 2003-09-16 | Auburn University | Phage ligand sensor devices and uses thereof |
US7166463B2 (en) * | 2001-11-16 | 2007-01-23 | The Regents Of The University Of Colorado | Nucleic acids encoding modified olfactory cyclic nucleotide gated ion channels |
WO2003078565A1 (en) | 2002-03-12 | 2003-09-25 | Surface Logix, Inc. | Cell motility and chemotaxis test device and methods of using same |
US7074311B1 (en) * | 2002-05-08 | 2006-07-11 | Sru Biosystems Inc. | Biosensor electrophoresis |
EP1549953A4 (en) | 2002-05-22 | 2006-12-06 | Platypus Technologies Inc | SUBSTRATES, DEVICES AND METHOD FOR CELL TESTS |
US7202343B2 (en) | 2002-08-19 | 2007-04-10 | Abgenix, Inc. | Antibodies directed to monocyte chemo-attractant protein-1 (MCP-1) and uses thereof |
US7927822B2 (en) | 2002-09-09 | 2011-04-19 | Sru Biosystems, Inc. | Methods for screening cells and antibodies |
US20040091397A1 (en) | 2002-11-07 | 2004-05-13 | Corning Incorporated | Multiwell insert device that enables label free detection of cells and other objects |
US7309614B1 (en) | 2002-12-04 | 2007-12-18 | Sru Biosystems, Inc. | Self-referencing biodetection method and patterned bioassays |
AU2003298169A1 (en) | 2003-03-24 | 2004-10-18 | Schering Ag | Modulators of the megalin-mediated uptake of radiotherapeutics and/or radiodiagnostics into kidney cells and their use in therapy and diagnostics |
US7497992B2 (en) | 2003-05-08 | 2009-03-03 | Sru Biosystems, Inc. | Detection of biochemical interactions on a biosensor using tunable filters and tunable lasers |
US8298780B2 (en) | 2003-09-22 | 2012-10-30 | X-Body, Inc. | Methods of detection of changes in cells |
EP1680679A2 (en) | 2003-11-06 | 2006-07-19 | SRU Biosystems Inc. | High-density amine-functionalized surface |
US20050208595A1 (en) | 2004-03-19 | 2005-09-22 | Brown Arthur M | High throughput assay systems and methods for identifying agents that alter expression of cellular proteins |
US6990259B2 (en) * | 2004-03-29 | 2006-01-24 | Sru Biosystems, Inc. | Photonic crystal defect cavity biosensor |
WO2006085958A2 (en) | 2004-06-28 | 2006-08-17 | Sru Biosystems, Inc. | Integration of direct binding sensors with mass spectrometry for functional and structural characterization of molecules |
US7803619B2 (en) | 2004-11-24 | 2010-09-28 | Geneprotech, Inc. | Embryoid body-based screen |
NZ562794A (en) | 2005-04-12 | 2010-07-30 | Sru Biosystems Inc | Proteolipid membrane & lipid membrane biosensor using a titanium surface |
US20070299029A1 (en) | 2005-06-09 | 2007-12-27 | Agency For Science, Technology And Research | Hepatic stellate cell specific promoter and uses thereof |
US7197198B2 (en) * | 2005-06-23 | 2007-03-27 | Sru Biosystems, Inc. | Biosensor substrate structure for reducing the effects of optical interference |
US7162125B1 (en) * | 2005-06-23 | 2007-01-09 | Sru Biosystems, Inc. | Optimized grating based biosensor and substrate combination |
US7479404B2 (en) | 2005-07-08 | 2009-01-20 | The Board Of Trustees Of The University Of Illinois | Photonic crystal biosensor structure and fabrication method |
US7521769B2 (en) | 2005-07-08 | 2009-04-21 | Sru Biosystems, Inc. | Photonic crystal biosensor structure and fabrication method |
US7483127B1 (en) | 2005-08-08 | 2009-01-27 | Sru Biosystems, Inc. | Method and apparatus for generating an image of biomolecular sensor target area |
EP1913366B1 (en) | 2005-08-11 | 2010-06-09 | SRU Biosystems, Inc. | Grating-based sensor combining label-free binding detection and fluorescence amplification |
US7790406B2 (en) | 2005-08-11 | 2010-09-07 | Sru Biosystems, Inc | Grating-based sensor combining label-free binding detection and fluorescence amplification and readout system for sensor |
CA2626852A1 (en) | 2005-11-03 | 2007-05-18 | Redpoint Bio Corporation | High throughput screening assay for the trpm5 ion channel |
US20070281864A1 (en) * | 2006-06-06 | 2007-12-06 | King Glenn F | Methods of identifying insect-specific spider toxin mimics |
WO2008008247A2 (en) | 2006-07-07 | 2008-01-17 | The Board Of Trustees Of The University Of Illinois | Near ultraviolet-wavelength photonic-crystal biosensor with enhanced surface to bulk sensitivity ratio |
US20080213910A1 (en) | 2006-10-31 | 2008-09-04 | Gangadhar Jogikalmath | Method for blocking non-specific protein binding on a functionalized surface |
JP5166430B2 (ja) | 2006-11-09 | 2013-03-21 | ザ・ボード・オブ・トラスティーズ・オブ・ザ・ユニバーシティ・オブ・イリノイ | 統合された流体含有構造を有するフォトニック結晶センサ |
US7628085B2 (en) | 2006-11-17 | 2009-12-08 | Sru Biosystems, Inc. | Simultaneous aspirator and dispenser for multiwell plates and similar devices |
US20080240543A1 (en) | 2007-03-30 | 2008-10-02 | Wolfgang Ernst Gustav Budach | Calibration and normalization method for biosensors |
CA2683082A1 (en) | 2007-04-19 | 2008-10-30 | Sru Biosystems, Inc. | Method for employing a biosensor to detect small molecules that bind directly to immobilized targets |
US9778267B2 (en) | 2007-07-11 | 2017-10-03 | X-Body, Inc. | Methods for identifying modulators of ion channels |
US9134307B2 (en) | 2007-07-11 | 2015-09-15 | X-Body, Inc. | Method for determining ion channel modulating properties of a test reagent |
US8268637B2 (en) | 2008-01-11 | 2012-09-18 | The Board Of Trustees Of The University Of Illinois | Label-free biosensors based upon distributed feedback laser |
WO2009114133A1 (en) | 2008-03-10 | 2009-09-17 | The J. David Gladstone Institutes | Cells and assays for use in detecting long qt syndrome |
US8257936B2 (en) | 2008-04-09 | 2012-09-04 | X-Body Inc. | High resolution label free analysis of cellular properties |
US20100015721A1 (en) | 2008-06-04 | 2010-01-21 | Sru Biosystems, Inc. | Detection of Promiscuous Small Submicrometer Aggregates |
US20100008826A1 (en) | 2008-07-10 | 2010-01-14 | Sru Biosystems, Inc. | Biosensors featuring confinement of deposited material and intra-well self-referencing |
CA2730415A1 (en) * | 2008-07-11 | 2010-01-14 | Sru Biosystems, Inc. | Methods for identifying modulators of ion channels |
US8094305B2 (en) | 2009-02-02 | 2012-01-10 | Sru Biosystems, Inc. | Efficient optical arrangement for illumination and detection of label-free biosensors and method to reduce interference fringes in label-free imaging |
CA2761114A1 (en) | 2009-05-15 | 2010-11-18 | Sru Biosystems, Inc. | Detection of changes in cell populations and mixed cell populations |
-
2008
- 2008-07-11 US US12/171,475 patent/US9778267B2/en active Active
- 2008-07-11 AU AU2008274978A patent/AU2008274978A1/en not_active Abandoned
- 2008-07-11 CA CA2693700A patent/CA2693700A1/en not_active Abandoned
- 2008-07-11 CN CN200880107175A patent/CN101802611A/zh active Pending
- 2008-07-11 NZ NZ582473A patent/NZ582473A/en not_active IP Right Cessation
- 2008-07-11 EP EP08796154A patent/EP2171454B1/en not_active Not-in-force
- 2008-07-11 JP JP2010516262A patent/JP2010532998A/ja active Pending
- 2008-07-11 WO PCT/US2008/069752 patent/WO2009009718A1/en active Application Filing
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2005516210A (ja) * | 2002-01-28 | 2005-06-02 | エス アール ユー バイオシステムス,エル エル シー | 線形光学表面構造を用いた導波モード共鳴フィルターバイオセンサー |
US20030224369A1 (en) * | 2002-02-25 | 2003-12-04 | Surber Mark W. | Reverse screening and target identification with minicells |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2012526998A (ja) * | 2009-05-15 | 2012-11-01 | エス アール ユー バイオシステムズ,インコーポレイテッド | 細胞集団および混合細胞集団における変化の検出 |
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WO2009009718A1 (en) | 2009-01-15 |
EP2171454A1 (en) | 2010-04-07 |
CN101802611A (zh) | 2010-08-11 |
CA2693700A1 (en) | 2009-01-15 |
EP2171454A4 (en) | 2010-08-04 |
EP2171454B1 (en) | 2013-03-20 |
AU2008274978A1 (en) | 2009-01-15 |
US20090017488A1 (en) | 2009-01-15 |
US9778267B2 (en) | 2017-10-03 |
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