WO2005000883A8 - Polypeptides having binding affinity for insulin - Google Patents

Polypeptides having binding affinity for insulin

Info

Publication number
WO2005000883A8
WO2005000883A8 PCT/SE2004/001050 SE2004001050W WO2005000883A8 WO 2005000883 A8 WO2005000883 A8 WO 2005000883A8 SE 2004001050 W SE2004001050 W SE 2004001050W WO 2005000883 A8 WO2005000883 A8 WO 2005000883A8
Authority
WO
WIPO (PCT)
Prior art keywords
insulin
polypeptide
sample
binding affinity
polypeptides
Prior art date
Application number
PCT/SE2004/001050
Other languages
French (fr)
Other versions
WO2005000883A1 (en
Inventor
Elin Gunneriusson
Joachim Feldwisch
Per-Aake Nygren
Original Assignee
Affibody Ab
Elin Gunneriusson
Joachim Feldwisch
Per-Aake Nygren
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Affibody Ab, Elin Gunneriusson, Joachim Feldwisch, Per-Aake Nygren filed Critical Affibody Ab
Publication of WO2005000883A1 publication Critical patent/WO2005000883A1/en
Publication of WO2005000883A8 publication Critical patent/WO2005000883A8/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/26—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against hormones ; against hormone releasing or inhibiting factors
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P5/00—Drugs for disorders of the endocrine system
    • A61P5/48—Drugs for disorders of the endocrine system of the pancreatic hormones
    • A61P5/50—Drugs for disorders of the endocrine system of the pancreatic hormones for increasing or potentiating the activity of insulin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/305—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Micrococcaceae (F)
    • C07K14/31—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Micrococcaceae (F) from Staphylococcus (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2318/00—Antibody mimetics or scaffolds
    • C07K2318/20—Antigen-binding scaffold molecules wherein the scaffold is not an immunoglobulin variable region or antibody mimetics
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Diabetes (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Endocrinology (AREA)
  • Engineering & Computer Science (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

A polypeptide, which has a binding affinity for insulin such that the KD value of the interaction is at most 1 x 10-6 M is provided. The polypeptide is related to a domain of staphylococcal protein A (SPA) in that the sequence of the polypeptide corresponds to the sequence of the SPA domain having from 1 to about 20 substitution mutations. Nucleic acid encoding the polypeptide, expression vector comprising the nucleic acid, and host cell comprising the expression vector are additionally provided. Also provided is a method of separation of insulin present in a sample from other constituents in the sample, which method comprises a step of affinity separation, in which step a polypeptide according to the invention is used. Also provided is a method of detection of insulin in a sample, in which method a polypeptide according to the invention is used.
PCT/SE2004/001050 2003-06-30 2004-06-30 Polypeptides having binding affinity for insulin WO2005000883A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
SE0301936A SE0301936D0 (en) 2003-06-30 2003-06-30 New polypeptide
SE0301936-1 2003-06-30

Publications (2)

Publication Number Publication Date
WO2005000883A1 WO2005000883A1 (en) 2005-01-06
WO2005000883A8 true WO2005000883A8 (en) 2005-03-03

Family

ID=27731066

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/SE2004/001050 WO2005000883A1 (en) 2003-06-30 2004-06-30 Polypeptides having binding affinity for insulin

Country Status (2)

Country Link
SE (1) SE0301936D0 (en)
WO (1) WO2005000883A1 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0524788D0 (en) 2005-12-05 2006-01-11 Affibody Ab Polypeptides
EP2183275B1 (en) 2007-08-03 2014-10-29 Affibody AB Igf-1r binding polypeptides and their use
GB0805608D0 (en) * 2008-03-28 2008-04-30 Sec Dep For Environment Food & Detection method
US20120165650A1 (en) 2010-12-22 2012-06-28 General Electric Company Her2 binders
EP2400990A2 (en) 2009-02-26 2012-01-04 OSI Pharmaceuticals, LLC In situ methods for monitoring the emt status of tumor cells in vivo
WO2014013016A1 (en) 2012-07-20 2014-01-23 Affibody Ab Method for determining the her2 status of a malignancy

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE8204810L (en) * 1982-08-23 1984-02-24 Pharmacia Fine Chemicals Ab HYBRID DNA MOLECULE, TRANSFORMED MICROORGANISM AND PROCEDURE TO MAKE PROTEIN A
AU8957091A (en) * 1990-11-26 1992-06-25 Public Health Laboratory Service Board Immunoglobulin-binding proteins and recombinant dna molecules coding therefor
US6013763A (en) * 1996-06-04 2000-01-11 Genentech, Inc. Peptide variants of protein A
GB9823071D0 (en) * 1998-10-21 1998-12-16 Affibody Technology Ab A method
SE9901379D0 (en) * 1999-04-19 1999-04-19 Pharmacia & Upjohn Ab Receptor structures
WO2001062907A1 (en) * 2000-02-22 2001-08-30 Medical & Biological Laboratories Co., Ltd. Antibody library
EP1314740A4 (en) * 2000-06-14 2005-01-19 Med & Biological Lab Co Ltd METHOD OF CONSTRUCTING scFv ANTIBODY FUSED WITH FLUORESCENT PROTEIN
GB0017720D0 (en) * 2000-07-19 2000-09-06 Got A Gene Ab Modified virus
SE0200943D0 (en) * 2002-03-25 2002-03-25 Amersham Biosciences Ab Mutant protein

Also Published As

Publication number Publication date
SE0301936D0 (en) 2003-06-30
WO2005000883A1 (en) 2005-01-06

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