ATE135403T1 - Herstellung und reinigung eines proteins, das mit einem bindungsprotein fusioniert ist - Google Patents

Herstellung und reinigung eines proteins, das mit einem bindungsprotein fusioniert ist

Info

Publication number
ATE135403T1
ATE135403T1 AT88302039T AT88302039T ATE135403T1 AT E135403 T1 ATE135403 T1 AT E135403T1 AT 88302039 T AT88302039 T AT 88302039T AT 88302039 T AT88302039 T AT 88302039T AT E135403 T1 ATE135403 T1 AT E135403T1
Authority
AT
Austria
Prior art keywords
polypeptide
protein
dna
binding protein
hybrid
Prior art date
Application number
AT88302039T
Other languages
English (en)
Inventor
Chudi Guan
Hiroshi Inouye
Original Assignee
New England Biolabs Inc
Univ Temple
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by New England Biolabs Inc, Univ Temple filed Critical New England Biolabs Inc
Application granted granted Critical
Publication of ATE135403T1 publication Critical patent/ATE135403T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/24—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a MBP (maltose binding protein)-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AT88302039T 1987-03-10 1988-03-09 Herstellung und reinigung eines proteins, das mit einem bindungsprotein fusioniert ist ATE135403T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US2405387A 1987-03-10 1987-03-10

Publications (1)

Publication Number Publication Date
ATE135403T1 true ATE135403T1 (de) 1996-03-15

Family

ID=21818632

Family Applications (1)

Application Number Title Priority Date Filing Date
AT88302039T ATE135403T1 (de) 1987-03-10 1988-03-09 Herstellung und reinigung eines proteins, das mit einem bindungsprotein fusioniert ist

Country Status (7)

Country Link
EP (1) EP0286239B1 (de)
JP (1) JP2703770B2 (de)
CN (1) CN88101209A (de)
AT (1) ATE135403T1 (de)
AU (1) AU626521B2 (de)
CA (1) CA1340173C (de)
DE (2) DE286239T1 (de)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5137819A (en) * 1988-07-08 1992-08-11 University Of British Columbia Cellulose binding fusion proteins for immobilization and purification of polypeptides
US5340731A (en) * 1988-07-08 1994-08-23 University Of British Columbia Method of preparing a B-1,4 glycan matrix containing a bound fusion protein
US5223409A (en) * 1988-09-02 1993-06-29 Protein Engineering Corp. Directed evolution of novel binding proteins
US7413537B2 (en) 1989-09-01 2008-08-19 Dyax Corp. Directed evolution of disulfide-bonded micro-proteins
WO1991010910A2 (de) * 1990-01-19 1991-07-25 Boehringer Mannheim Gmbh Verfahren zur enzymatischen behandlung von substraten
US5595887A (en) * 1990-07-16 1997-01-21 Bionebraska, Inc. Purification directed cloning of peptides using carbonic anhydrase as the affinity binding segment
EP1279731B1 (de) 1991-03-01 2007-05-30 Dyax Corporation Verfahren zur Herstellung bindender Miniproteine
US5440025A (en) * 1992-03-12 1995-08-08 University Of Massachusetts At Lowell Process for separating nucleic acid polymers
US5482850A (en) * 1993-10-29 1996-01-09 New England Biolabs, Inc. Method for identifying anti-parasitic compounds
CA2151547C (en) 1994-06-10 2010-10-12 Elliott A. Gruskin Recombinant chimeric proteins and methods of use thereof
GB9423367D0 (en) * 1994-11-18 1995-01-11 Wellcome Found Enzyme prodrug therapy
WO1996017941A2 (en) * 1994-12-07 1996-06-13 Bionebraska, Inc. Production of c-terminal amidated peptides from recombinant protein constructs
US7045363B2 (en) 1996-05-01 2006-05-16 Fujirebio Inc. Nucleic acid-bound polypeptide method of producing nucleic acid-bound polypeptide and immunoassay using the polypeptide
US6492508B1 (en) 1996-06-03 2002-12-10 United States Surgical Corp. A Division Of Tyco Healthcare Group Nucleic acids encoding extracellular matrix proteins
US6303575B1 (en) 1998-05-12 2001-10-16 The Regents Of The University Of California Indolicidin analogs and methods of using same
DE19944626A1 (de) * 1999-09-17 2001-03-22 Knoell Hans Forschung Ev Verfahren zur Herstellung von dimeren, biologisch aktiven knochenmorphogenetischen Proteinen
EP1242599A2 (de) * 1999-12-14 2002-09-25 Societe De Conseils De Recherches Et D'applications Scientifiques (S.C.R.A.S.) Methode zur isolierung von menschlichen cdc25 phosphatasen und verfahren zur identifizierung von deren modulatoren
DE60230594D1 (de) * 2001-02-26 2009-02-12 Toyo Boseki Fusionsprotein mit beta-1,2-n-acetylglucosaminyl-transferase-ii-aktivität und herstellungsverfahren dafür
EP1335003A3 (de) 2002-02-06 2004-04-07 Toyo Boseki Kabushiki Kaisha Magnetischer Träger der in der Lage ist Proteine zu binden und Verfahren zur Reinigung von Proteinen den magnetischen Träger verwendend
US7807174B2 (en) * 2002-11-22 2010-10-05 Nexbio, Inc. Class of therapeutic protein based molecules
WO2004074488A1 (en) * 2003-02-21 2004-09-02 Biotecnol S.A. Use of caspase enzymes for maturation of engineered recombinant polypeptide fusions
MY142798A (en) * 2003-03-04 2010-12-31 Smithkline Beecham Corp Methods for preventing glyconoylation of proteins
CL2016000572A1 (es) * 2016-03-10 2016-12-02 Univ Santo Tomás Proteína vainillil alcohol oxidasa recombinante (vaor)
CN113480632B (zh) * 2021-07-30 2023-02-03 宁波人健药业集团股份有限公司 一种在CHO细胞中表达的重组蛋白rhCG纯化工艺

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FI86437C (fi) * 1978-12-22 1992-08-25 Biogen Inc Foerfarande foer framstaellning av polypeptider med de antigena egenskaperna hos ett hepatitis b- virusantigen.
DE3410437A1 (de) * 1984-03-22 1985-09-26 Bayer Ag, 5090 Leverkusen Verfahren zur herstellung von proteinen
EP0195680A3 (de) * 1985-03-21 1987-06-10 Immunex Corporation Proteinsynthese mit einem Identifizierungspeptid
EP0244147A3 (de) * 1986-04-28 1989-04-05 Merck & Co. Inc. Verfahren zur Reinigung hybrider Proteine

Also Published As

Publication number Publication date
EP0286239B1 (de) 1996-03-13
CA1340173C (en) 1998-12-08
DE286239T1 (de) 1994-02-24
JP2703770B2 (ja) 1998-01-26
EP0286239A1 (de) 1988-10-12
DE3855084D1 (de) 1996-04-18
AU1283288A (en) 1988-09-08
AU626521B2 (en) 1992-08-06
DE3855084T2 (de) 1996-09-12
CN88101209A (zh) 1988-12-21
JPS6420094A (en) 1989-01-24

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Legal Events

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