KR850006550A - 단백질의 제조방법 - Google Patents

단백질의 제조방법 Download PDF

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Publication number
KR850006550A
KR850006550A KR1019850001856A KR850001856A KR850006550A KR 850006550 A KR850006550 A KR 850006550A KR 1019850001856 A KR1019850001856 A KR 1019850001856A KR 850001856 A KR850001856 A KR 850001856A KR 850006550 A KR850006550 A KR 850006550A
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South Korea
Prior art keywords
protein
biospecific
polypeptide
short chain
bound
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KR1019850001856A
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English (en)
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슈미트-카스트너 귄터 (외 1)
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루디 마이어, 요아힘 그렘
바이엘 악티엔게젤샤프트
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Publication of KR850006550A publication Critical patent/KR850006550A/ko

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand

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  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

내용 없음

Description

단백질의 제조방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
본 발명에 따른 공정을 다이아그람식으로 도식한 것임.

Claims (10)

  1. a) 목적 단백질 암호인 DNA서열, 짧은 사슬펩티드 암호인 DNA서열 및 생체 특이성 폴리펩티드 암호인 DNA서열을 짧은 사슬 펩티드용 DNA서열이 다른 두 DNA서열 사이에 위치하도록 서로 결합시키고, b) 생성된 새로운 DNA 단편은 생체특이성 폴리펩티드, 짧은 사슬 펩티드 및 목적 단백질로된 혼성화 단백질의 전사, 해독 및 표현과정이 일어나도록 적당한 유전공학기법에 의하여 숙주 미생물의 DNA에 도입시키고, c) 숙주 미생물이 혼성화 단백질을 생성하도록 배양시키고, d) 혼성화 단백질을 함유한 배양계부분을 필요한다면 다른 구성성분이 제거후에 적당한 담체물질로 이루어진 고정계와 접촉시키고, 여기에 생체 특이성 폴리펩티드에 상보성(相補性)이고, 소정의 반응조건에서 그 폴리펩티드에 강력하게 결합되는 물질을 결합시키고, e) 혼성화 단백질이 결합되어 있는 고정계를 적당한 방법으로 다른 구성성분으로부터 분리시켜서 그 결합된 혼성화단백질로부터 짧은 사슬펩티드를 특이적으로 절달시키는 시약으로 처리하고, f) 이와 같이 하여 유리시킨 목적 단백질을 다른 구성 성분으로부터 분리해 내어 적당한 방법으로 단리시키는 것을 특징으로 하는 단백질 제조방법.
  2. 제1항에 있어서, 생체 특이성 폴리펩티드가 높은 비율로 보체(補體)를 결합시킨 담체에 높은 비율로 결합됨을 특징으로 하는 방법.
  3. 제2항에 있어서, 결합이 +2℃에서 실온까지의 온도에서 형성됨을 특징으로 하는 방법.
  4. 제1 내지 제3항에 있어서, 생체 특이성 폴리펩티드와 상보성의 담체 결합 물질이 효소 및 관련 억제제로 이루어진 계임을 특징으로 하는 방법.
  5. 제1 내지 제4항에 있어서, 생체 특이성 폴리펩티드가 아프로티닌이고, 상보성의 담체 결합 물질이 트립신임을 특징으로 하는 방법.
  6. 제1 내지 제5항에 있어서, 짧은 사슬 펩티드가 Lys-Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg 데카펩티드임을 특징으로 하는 방법.
  7. 제1 내지 제6항에 있어서, 결합된 혼성화 단백질로부터 특이적으로 짧은 사슬펩티드를 절단시키는 시약이 효소임을 특징으로 하는 방법.
  8. 제6 내지 제7항에 있어서, 효소가 칼리크레인(E.C. 2.4.21.8)임을 특징으로 하는 방법.
  9. 제1 내지 제8항에 있어서, 제조될 단백질이 호르몬, 플라스마단백질, 응고화 인자, 효소, 항체 또는 백신류임을 특징으로 하는 방법.
  10. 제 9항에 있어서, 단백질이 인자 Ⅷ임을 특징으로 하는 방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019850001856A 1984-03-22 1985-03-21 단백질의 제조방법 KR850006550A (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19843410437 DE3410437A1 (de) 1984-03-22 1984-03-22 Verfahren zur herstellung von proteinen
DEP3410437.2 1984-03-22

Publications (1)

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KR850006550A true KR850006550A (ko) 1985-10-14

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Country Status (7)

Country Link
EP (1) EP0157235A1 (ko)
JP (1) JPS60214897A (ko)
KR (1) KR850006550A (ko)
CA (1) CA1230840A (ko)
DE (1) DE3410437A1 (ko)
DK (1) DK129085A (ko)
IL (1) IL74661A0 (ko)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100792510B1 (ko) * 2007-10-29 2008-01-08 이강운 직교 좌표형 로봇의 듀얼타입 테이블

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE63757T1 (de) * 1985-03-28 1991-06-15 Chiron Corp Expression durch verwendung von fusionsgenen fuer proteinproduktion.
DE3523701A1 (de) * 1985-07-03 1987-01-08 Bayer Ag Verfahren zur herstellung von proteinen und polypeptiden
EP0212285A1 (en) * 1985-07-29 1987-03-04 Arthur A. Gottlieb Derivation and use of selected gene products
EP0241546A4 (en) * 1985-10-11 1988-11-02 Genetics Inst METHOD FOR PRODUCING HETEROLOGICAL PROTEINS.
GB2188322A (en) * 1986-03-26 1987-09-30 Bayer Ag Aprotinin and analogues thereof produced by a recombinant host
GB2188933A (en) * 1986-04-10 1987-10-14 Bayer Ag Expression vectors for production of polypeptides, method for enhanced expression of polypeptides, hosts containing the expression vectors, products manufactured thereby
CA1340173C (en) * 1987-03-10 1998-12-08 Chudi Guan Production and purification of a protein fused to a binding protein
US5032573A (en) * 1987-03-23 1991-07-16 Bayer Aktiengesellschaft Homologs of aprotinin produced from a recombinant host, process, expression vector and recombinant host therefor and pharmaceutical use thereof
FR2615528B1 (fr) * 1987-05-18 1991-05-24 Pasteur Institut Procede de production d'un polypeptide ayant des proprietes choisies determinees, dans un organisme-hote transforme par un adn recombinant contenant des sequences nucleiques codant pour ce polypeptide et pour une proteine affine d'ose
DE3731874A1 (de) * 1987-09-18 1989-03-30 Schering Ag Verfahren zur herstellung von peptiden durch spezifische spaltung von gentechnisch gewonnenen fusionsproteinen mit collagenasen
SE8800981A (sv) * 1988-03-17 1988-10-14 Lars Abrahamsen A recombinant fusion protein, its use and a recombinant vector
US5137819A (en) * 1988-07-08 1992-08-11 University Of British Columbia Cellulose binding fusion proteins for immobilization and purification of polypeptides
EP0372352B1 (en) * 1988-12-06 1995-06-07 Chisso Corporation Aequorin fused with a protein having a specific-binding activity, its preparation, its purification and detection method by its use
GB8927722D0 (en) * 1989-12-07 1990-02-07 British Bio Technology Proteins and nucleic acids
WO1991011454A1 (en) * 1990-01-24 1991-08-08 The Upjohn Company Method of purifying recombinant polypeptides
CN103285814B (zh) * 2012-11-30 2015-07-08 西北大学 一种基于强螯合配体的固定金属亲和色谱固定相及其制备方法

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2963374D1 (en) * 1978-10-10 1982-09-09 Univ Leland Stanford Junior Recombinant dna, method for preparing it and production of foreign proteins by unicellular hosts containing it

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100792510B1 (ko) * 2007-10-29 2008-01-08 이강운 직교 좌표형 로봇의 듀얼타입 테이블

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JPS60214897A (ja) 1985-10-28
CA1230840A (en) 1987-12-29
DK129085D0 (da) 1985-03-21
IL74661A0 (en) 1985-06-30
DK129085A (da) 1985-09-23
DE3410437A1 (de) 1985-09-26
EP0157235A1 (de) 1985-10-09

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