EA200100160A1 - Новые способы идентификации биомолекул лигандов и мишеней - Google Patents

Новые способы идентификации биомолекул лигандов и мишеней

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Publication number
EA200100160A1
EA200100160A1 EA200100160A EA200100160A EA200100160A1 EA 200100160 A1 EA200100160 A1 EA 200100160A1 EA 200100160 A EA200100160 A EA 200100160A EA 200100160 A EA200100160 A EA 200100160A EA 200100160 A1 EA200100160 A1 EA 200100160A1
Authority
EA
Eurasian Patent Office
Prior art keywords
cells
peptide
rna
methods
enzyme
Prior art date
Application number
EA200100160A
Other languages
English (en)
Other versions
EA003637B1 (ru
Inventor
Торбен Халькиер
Лене Есперсен
Аллан Енсен
Original Assignee
М Энд Е Байотек А/С
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by М Энд Е Байотек А/С filed Critical М Энд Е Байотек А/С
Publication of EA200100160A1 publication Critical patent/EA200100160A1/ru
Publication of EA003637B1 publication Critical patent/EA003637B1/ru

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1044—Preparation or screening of libraries displayed on scaffold proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1051—Gene trapping, e.g. exon-, intron-, IRES-, signal sequence-trap cloning, trap vectors

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Analytical Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

Abstract

Данное изобретение обеспечивает способы идентификации биологически активных биомолекул. В одном аспекте биологически активную биомолекулу, такую как РНК или пептид, идентифицируют включением случайных нуклеотидных последовательностей в каркас, состоящий из модулятора активности фермента, трансформацией существенно идентичных клеток полученной таким образом конструкцией и скринингом трансформированных клеток для идентификации клеток, в которых был изменен предварительно выбранный фенотипический признак. Затем из фенотипически измененных клеток выделяют рандомизированную ДНК и определяют пептид и/или РНК, кодируемые этой случайной последовательностью. В свою очередь, идентифицируют партнеры взаимодействия, которые являются возможными лекарственными средствами-мишенями, и выделяют их с использованием указанных пептида и/или РНК в качестве части аффинных реагентов. Предпочтительную каркасную молекулу получают из семейства ингибиторов протеаз ингибиторов I картофеля, примером которых является ингибитор трипсина 2A ячменя (CI-2A). Другой аспект относится к идентификации новых ингибиторов ферментов с использованием, по существу, того же самого подхода, но с проведением специфического скрининга на изменения в активности фермента-мишени. Описаны также способы получения соответствующих трансформирующих и экспрессирующих векторов, а также способы идентификации соединений-примеров и лекарственных средств-мишеней для применения в разработке лекарственных средств. Наконец, данное изобретение включает также в свой объем способ получения лекарственного продукта.Международная заявка была опубликована
EA200100160A 1998-07-20 1999-07-16 Новые способы идентификации биомолекул лигандов и мишеней EA003637B1 (ru)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DKPA199800956 1998-07-20
US9486898P 1998-07-29 1998-07-29
PCT/DK1999/000408 WO2000005406A1 (en) 1998-07-20 1999-07-16 Novel methods for the identification of ligand and target biomolecules

Publications (2)

Publication Number Publication Date
EA200100160A1 true EA200100160A1 (ru) 2001-06-25
EA003637B1 EA003637B1 (ru) 2003-08-28

Family

ID=26064970

Family Applications (1)

Application Number Title Priority Date Filing Date
EA200100160A EA003637B1 (ru) 1998-07-20 1999-07-16 Новые способы идентификации биомолекул лигандов и мишеней

Country Status (17)

Country Link
EP (1) EP1098991B1 (ru)
JP (1) JP2002521652A (ru)
KR (1) KR20010103560A (ru)
AT (1) ATE223971T1 (ru)
AU (1) AU751055B2 (ru)
CA (1) CA2335343A1 (ru)
DE (1) DE69902924T2 (ru)
DK (1) DK1098991T3 (ru)
EA (1) EA003637B1 (ru)
EE (1) EE200100040A (ru)
HU (1) HUP0102457A2 (ru)
NO (1) NO20010300L (ru)
NZ (1) NZ509013A (ru)
PL (1) PL346126A1 (ru)
SK (1) SK692001A3 (ru)
TR (1) TR200100206T2 (ru)
WO (1) WO2000005406A1 (ru)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6365344B1 (en) 1996-01-23 2002-04-02 The Board Of Trustees Of The Leland Stanford Junior University Methods for screening for transdominant effector peptides and RNA molecules
CA2244222A1 (en) 1996-01-23 1997-07-31 The Board Of Trustees Of The Leland Stanford Junior University Methods for screening for transdominant effector peptides and rna molecules
WO2001059071A2 (en) 2000-02-09 2001-08-16 Genvec, Inc. Methods of preparing and using a viral vector library
EP1373496A4 (en) * 2001-02-22 2004-10-27 Praecis Pharm Inc METHODS OF IDENTIFYING PEPTIDES MODULATING A BIOLOGICAL PROCESS
US6872544B2 (en) 2001-09-25 2005-03-29 Kemin Consumer Care, L.C. Raw material selection and analysis for the isolation of proteinase inhibitor II from whole potatoes
JP4834554B2 (ja) 2003-11-06 2011-12-14 ジェネンコー・インターナショナル・インク 糸状菌におけるプロテアーゼ抑制剤及びその変異体の発現
ATE486087T1 (de) * 2003-11-06 2010-11-15 Danisco Us Inc Fgf-5-bindende und geträgerte peptide
DK1904529T3 (da) * 2005-06-10 2010-11-15 Medical Res Council Anvendelse af Stefin A som et scaffoldprotein
US8043830B2 (en) * 2008-02-01 2011-10-25 The Regents Of The University Of California Biotin-ligase system for secretion of biotinylated protein
US7772181B2 (en) 2008-10-15 2010-08-10 Danisco Us Inc. Personal care compositions comprising modified variant Bowman Birk Protease Inhibitors
US7803902B2 (en) 2008-10-15 2010-09-28 Danisco Us Inc. Modified variant bowman birk protease inhibitors
MX2011003803A (es) * 2008-10-15 2011-07-04 Danisco Inc Inhibidores de proteasa de bowman birk, variantes, modificados.
CN103808945B (zh) * 2014-03-12 2016-02-10 山东农业大学 一种j亚群禽白血病免疫胶体金抗体检测试纸条
WO2016172445A2 (en) * 2015-04-24 2016-10-27 The University Of Florida Research Foundation, Inc. Methods of identifying biologically active random peptides in plants and libraries of plants expressing candidate biologically active random peptides
US10876109B2 (en) 2015-04-24 2020-12-29 University Of Florida Research Foundation, Inc. Methods of identifying biologically active random peptides in prokaryotic cells and libraries of prokaryotic cells expressing candidate biologically active random peptides
US10801023B2 (en) * 2015-04-24 2020-10-13 University Of Florida Research Foundation, Inc. Methods of identifying biologically active random peptides in plants and libraries of plants expressing candidate biologically active random peptides

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996038553A1 (en) * 1995-06-02 1996-12-05 M&E Biotech A/S A method for identification of biologically active peptides and nucleic acids
CA2244222A1 (en) * 1996-01-23 1997-07-31 The Board Of Trustees Of The Leland Stanford Junior University Methods for screening for transdominant effector peptides and rna molecules
AU5927598A (en) * 1997-01-23 1998-08-18 Immusol Incorporated Gene functional analysis and discovery using randomized or target-specific ribozyme gene vector libraries

Also Published As

Publication number Publication date
AU751055B2 (en) 2002-08-08
TR200100206T2 (tr) 2001-06-21
DE69902924D1 (de) 2002-10-17
NO20010300D0 (no) 2001-01-18
JP2002521652A (ja) 2002-07-16
PL346126A1 (en) 2002-01-28
WO2000005406A1 (en) 2000-02-03
CA2335343A1 (en) 2000-02-03
NO20010300L (no) 2001-03-19
DK1098991T3 (da) 2003-01-20
HUP0102457A2 (hu) 2001-10-28
EA003637B1 (ru) 2003-08-28
NZ509013A (en) 2002-12-20
EP1098991B1 (en) 2002-09-11
EP1098991A1 (en) 2001-05-16
EE200100040A (et) 2002-06-17
ATE223971T1 (de) 2002-09-15
SK692001A3 (en) 2001-12-03
AU4898599A (en) 2000-02-14
DE69902924T2 (de) 2003-05-28
KR20010103560A (ko) 2001-11-23

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Designated state(s): AM AZ BY KZ KG MD TJ TM RU