HUP0102457A2 - Új, biológiai ligandumok és célmolekulák azonosítására szolgáló eljárások - Google Patents

Új, biológiai ligandumok és célmolekulák azonosítására szolgáló eljárások

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Publication number
HUP0102457A2
HUP0102457A2 HU0102457A HUP0102457A HUP0102457A2 HU P0102457 A2 HUP0102457 A2 HU P0102457A2 HU 0102457 A HU0102457 A HU 0102457A HU P0102457 A HUP0102457 A HU P0102457A HU P0102457 A2 HUP0102457 A2 HU P0102457A2
Authority
HU
Hungary
Prior art keywords
procedures
production
identification
modulator
cells
Prior art date
Application number
HU0102457A
Other languages
English (en)
Inventor
Torben Halkier
Allan Jensen
Lene Jespersen
Original Assignee
M & E Biotech A/S
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by M & E Biotech A/S filed Critical M & E Biotech A/S
Publication of HUP0102457A2 publication Critical patent/HUP0102457A2/hu

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1044—Preparation or screening of libraries displayed on scaffold proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1051—Gene trapping, e.g. exon-, intron-, IRES-, signal sequence-trap cloning, trap vectors

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Analytical Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

Abstract

A találmány tárgyát képezik biológiai aktivitással rendelkezőbiomolekulák azonosítására szolgáló eljárások, amelyekbenenzimaktivitás-modulátort (vázmolekulaként) kódoló nukleotid-szekvenciába random peptideket illesztve alapvetően azonos sejtekettranszformálnak, és a transzformált sejteket előre kiválasztottfenotípusos elváltozás szempontjából szkrínelik. A randomizált DNS-szekvenciát a sejtekből izolálhatják és a random szekvenciátmeghatározhatják. A sejtben az expresszált fehérjével kölcsönhatásbalépő partnermolekulákat (feltételezett droghatás-célmolekulákként)azonosíthatják és izolálhatják a random peptidet affinitásireagensként alkalmazva. Vázmolekulaként 2A-típusú, árpaeredetűkimotripszin-inhibitort (CI-2A) alkalmaznak. Az eljárássalazonosíthatóak új enzim-inhibitorok és gyógyhatású készítményekbenalkalmazható drog kifejlesztésben alkalmazható lead-vegyületek. Atalálmány tárgyát képezik tehát: - eljárások olyan, biológiailag aktívpolipeptid-fragmens formájú modulátor azonosítására, amely alkalmassejtben fenotípusos sajátosság modulálására in vivo, detektálhatómódon történő modulálására; - eljárások replikációra képes vektorelőállítására - eljárások modulátor azonosítására, izolálására éselőállítására: - eljárások biológiai célmolekula azonosítására,izolálására és előállítására: - eljárások feltételezett drogjelöltkémiai vegyület kiválasztására; - eljárások gyógytermék előállítására. Ó
HU0102457A 1998-07-20 1999-07-16 Új, biológiai ligandumok és célmolekulák azonosítására szolgáló eljárások HUP0102457A2 (hu)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DKPA199800956 1998-07-20
US9486898P 1998-07-29 1998-07-29
PCT/DK1999/000408 WO2000005406A1 (en) 1998-07-20 1999-07-16 Novel methods for the identification of ligand and target biomolecules

Publications (1)

Publication Number Publication Date
HUP0102457A2 true HUP0102457A2 (hu) 2001-10-28

Family

ID=26064970

Family Applications (1)

Application Number Title Priority Date Filing Date
HU0102457A HUP0102457A2 (hu) 1998-07-20 1999-07-16 Új, biológiai ligandumok és célmolekulák azonosítására szolgáló eljárások

Country Status (17)

Country Link
EP (1) EP1098991B1 (hu)
JP (1) JP2002521652A (hu)
KR (1) KR20010103560A (hu)
AT (1) ATE223971T1 (hu)
AU (1) AU751055B2 (hu)
CA (1) CA2335343A1 (hu)
DE (1) DE69902924T2 (hu)
DK (1) DK1098991T3 (hu)
EA (1) EA003637B1 (hu)
EE (1) EE200100040A (hu)
HU (1) HUP0102457A2 (hu)
NO (1) NO20010300L (hu)
NZ (1) NZ509013A (hu)
PL (1) PL346126A1 (hu)
SK (1) SK692001A3 (hu)
TR (1) TR200100206T2 (hu)
WO (1) WO2000005406A1 (hu)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2000504220A (ja) 1996-01-23 2000-04-11 ライガル ファーマシューティカルズ インコーポレイテッド トランス支配性細胞内作用因子ペプチドおよびｒｎａ分子のスクリーニング方法
US6365344B1 (en) 1996-01-23 2002-04-02 The Board Of Trustees Of The Leland Stanford Junior University Methods for screening for transdominant effector peptides and RNA molecules
AU2001234980A1 (en) 2000-02-09 2001-08-20 Genvec, Inc. Methods of preparing and using a viral vector library
EP1373496A4 (en) * 2001-02-22 2004-10-27 Praecis Pharm Inc METHODS OF IDENTIFYING PEPTIDES MODULATING A BIOLOGICAL PROCESS
US6872544B2 (en) 2001-09-25 2005-03-29 Kemin Consumer Care, L.C. Raw material selection and analysis for the isolation of proteinase inhibitor II from whole potatoes
WO2005046709A2 (en) 2003-11-06 2005-05-26 Genencor International, Inc. Tgf - beta binding and supported peptides
CA2545053C (en) * 2003-11-06 2016-04-19 Genencor International, Inc. Expression in filamentous fungi of protease inhibitors and variants thereof
CA2610976C (en) * 2005-06-10 2020-11-24 Robbie Woodman Use of stefin a as a scaffold protein
US8043830B2 (en) * 2008-02-01 2011-10-25 The Regents Of The University Of California Biotin-ligase system for secretion of biotinylated protein
CN102245635B (zh) * 2008-10-15 2016-08-31 丹尼斯科美国公司 修饰的变体bowman birk蛋白酶抑制剂
US7772181B2 (en) 2008-10-15 2010-08-10 Danisco Us Inc. Personal care compositions comprising modified variant Bowman Birk Protease Inhibitors
US7803902B2 (en) 2008-10-15 2010-09-28 Danisco Us Inc. Modified variant bowman birk protease inhibitors
CN103808945B (zh) * 2014-03-12 2016-02-10 山东农业大学 一种j亚群禽白血病免疫胶体金抗体检测试纸条
WO2016172445A2 (en) * 2015-04-24 2016-10-27 The University Of Florida Research Foundation, Inc. Methods of identifying biologically active random peptides in plants and libraries of plants expressing candidate biologically active random peptides
US10876109B2 (en) 2015-04-24 2020-12-29 University Of Florida Research Foundation, Inc. Methods of identifying biologically active random peptides in prokaryotic cells and libraries of prokaryotic cells expressing candidate biologically active random peptides
US10801023B2 (en) * 2015-04-24 2020-10-13 University Of Florida Research Foundation, Inc. Methods of identifying biologically active random peptides in plants and libraries of plants expressing candidate biologically active random peptides

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2151167T3 (es) * 1995-06-02 2000-12-16 M & E Biotech As S Procedimiento para la identificacion de acidos nucleicos y peptidos biologicamente activos.
JP2000504220A (ja) * 1996-01-23 2000-04-11 ライガル ファーマシューティカルズ インコーポレイテッド トランス支配性細胞内作用因子ペプチドおよびｒｎａ分子のスクリーニング方法
WO1998032880A1 (en) * 1997-01-23 1998-07-30 Immusol Incorporated Gene functional analysis and discovery using randomized or target-specific ribozyme gene vector libraries

Also Published As

Publication number Publication date
SK692001A3 (en) 2001-12-03
EA200100160A1 (ru) 2001-06-25
NO20010300L (no) 2001-03-19
TR200100206T2 (tr) 2001-06-21
DE69902924T2 (de) 2003-05-28
EP1098991B1 (en) 2002-09-11
EA003637B1 (ru) 2003-08-28
AU4898599A (en) 2000-02-14
EP1098991A1 (en) 2001-05-16
JP2002521652A (ja) 2002-07-16
EE200100040A (et) 2002-06-17
NO20010300D0 (no) 2001-01-18
DK1098991T3 (da) 2003-01-20
NZ509013A (en) 2002-12-20
PL346126A1 (en) 2002-01-28
KR20010103560A (ko) 2001-11-23
DE69902924D1 (de) 2002-10-17
AU751055B2 (en) 2002-08-08
ATE223971T1 (de) 2002-09-15
WO2000005406A1 (en) 2000-02-03
CA2335343A1 (en) 2000-02-03

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