DE69729182D1 - Prozess zur chromatographischen trennung von peptiden und nucleinsaure und neue ionentauschermatrix mit hoher affinitat - Google Patents

Prozess zur chromatographischen trennung von peptiden und nucleinsaure und neue ionentauschermatrix mit hoher affinitat

Info

Publication number
DE69729182D1
DE69729182D1 DE69729182T DE69729182T DE69729182D1 DE 69729182 D1 DE69729182 D1 DE 69729182D1 DE 69729182 T DE69729182 T DE 69729182T DE 69729182 T DE69729182 T DE 69729182T DE 69729182 D1 DE69729182 D1 DE 69729182D1
Authority
DE
Germany
Prior art keywords
pct
ion exchanger
peptides
anion exchanger
high affinity
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69729182T
Other languages
English (en)
Other versions
DE69729182T2 (de
Inventor
Rolf Berglund
Jan Bergstroem
Makonnen Belew
Lennart Soederberg
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cytiva Sweden AB
Original Assignee
Amersham Bioscience AB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amersham Bioscience AB filed Critical Amersham Bioscience AB
Application granted granted Critical
Publication of DE69729182D1 publication Critical patent/DE69729182D1/de
Publication of DE69729182T2 publication Critical patent/DE69729182T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
    • B01D15/361—Ion-exchange
    • B01D15/363—Anion-exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J41/00—Anion exchange; Use of material as anion exchangers; Treatment of material for improving the anion exchange properties
    • B01J41/04—Processes using organic exchangers
    • B01J41/07—Processes using organic exchangers in the weakly basic form
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J41/00—Anion exchange; Use of material as anion exchangers; Treatment of material for improving the anion exchange properties
    • B01J41/20—Anion exchangers for chromatographic processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2220/00—Aspects relating to sorbent materials
    • B01J2220/50—Aspects relating to the use of sorbent or filter aid materials
    • B01J2220/54—Sorbents specially adapted for analytical or investigative chromatography

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Peptides Or Proteins (AREA)
DE69729182T 1996-02-19 1997-02-14 Prozess zur chromatographischen trennung von peptiden und nucleinsaure und neue ionentauschermatrix mit hoher affinität Expired - Lifetime DE69729182T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
SE9600590 1996-02-19
SE9600590A SE9600590D0 (sv) 1996-02-19 1996-02-19 Sätt för kromatografisk separation av peptider och nukleinsyra samt ny högaffin jonbytesmatris
PCT/SE1997/000237 WO1997029825A1 (en) 1996-02-19 1997-02-14 Process for chromatographic separation of peptides and nucleic acid, and new high affinity ion exchange matrix

Publications (2)

Publication Number Publication Date
DE69729182D1 true DE69729182D1 (de) 2004-06-24
DE69729182T2 DE69729182T2 (de) 2005-01-27

Family

ID=20401430

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69729182T Expired - Lifetime DE69729182T2 (de) 1996-02-19 1997-02-14 Prozess zur chromatographischen trennung von peptiden und nucleinsaure und neue ionentauschermatrix mit hoher affinität

Country Status (8)

Country Link
US (1) US6090288A (de)
EP (1) EP0888157B1 (de)
JP (1) JP4422797B2 (de)
AT (1) ATE267037T1 (de)
AU (1) AU713635B2 (de)
DE (1) DE69729182T2 (de)
SE (1) SE9600590D0 (de)
WO (1) WO1997029825A1 (de)

Families Citing this family (67)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9425138D0 (en) 1994-12-12 1995-02-08 Dynal As Isolation of nucleic acid
US6027945A (en) 1997-01-21 2000-02-22 Promega Corporation Methods of isolating biological target materials using silica magnetic particles
SE9700769D0 (sv) 1997-03-04 1997-03-04 Pharmacia Biotech Ab Matriser för separation och separation som utnyttjar matriserna
DE19736366A1 (de) * 1997-08-21 1999-02-25 Ernst Prof Dr Bayer Verfahren zur Isolierung von anionischen organischen Verbindungen aus wäßrigen Systemen mit kationischen Polymernanopartikeln
US6914137B2 (en) * 1997-12-06 2005-07-05 Dna Research Innovations Limited Isolation of nucleic acids
ATE386044T1 (de) * 1997-12-06 2008-03-15 Invitrogen Corp Isolierung von nukleinsäuren
US7078224B1 (en) 1999-05-14 2006-07-18 Promega Corporation Cell concentration and lysate clearance using paramagnetic particles
SE9802214D0 (sv) * 1998-06-18 1998-06-18 Amersham Pharm Biotech Ab Jonbytare och användning därav
US6441152B1 (en) * 1998-12-08 2002-08-27 Boston Probes, Inc. Methods, kits and compositions for the identification of nucleic acids electrostatically bound to matrices
WO2000044928A2 (en) * 1999-01-27 2000-08-03 Halaka Folim G Materials and methods for the purification of polyelectrolytes
US6270970B1 (en) 1999-05-14 2001-08-07 Promega Corporation Mixed-bed solid phase and its use in the isolation of nucleic acids
US6310199B1 (en) 1999-05-14 2001-10-30 Promega Corporation pH dependent ion exchange matrix and method of use in the isolation of nucleic acids
SE9904197D0 (sv) 1999-11-22 1999-11-22 Amersham Pharm Biotech Ab A method for anion exchange adsorption on matrices carrying mixed mode ligands
SE9904272D0 (sv) * 1999-11-25 1999-11-25 Amersham Pharm Biotech Ab A method for selective removal of a substance from samples containing compounds having nucleic acid structure
GB9927904D0 (en) * 1999-11-25 2000-01-26 Amersham Pharm Biotech Ab A method fro obtaining a nucleic acid variant
DE60124868T2 (de) * 2000-02-08 2007-06-28 Upfront Chromatography A/S, Kopenhagen Neue harze und ihre verwendung zur rückgewinnung von proteinen oder peptiden
US20020194901A1 (en) * 2000-12-19 2002-12-26 Showa Denko K.K. Porous polymer particle, alkali-resistant anion exchanger, producing method thereof, column for ion chromatography, and method for measuring anions
US20030066336A1 (en) * 2000-12-19 2003-04-10 Showa Denko K.K. Method for measuring anions
SE0004932D0 (sv) * 2000-12-31 2000-12-31 Apbiotech Ab A method for mixed mode adsorption and mixed mode adsorbents
DE60236951D1 (de) * 2001-05-21 2010-08-19 Fujifilm Corp Vorrichtung zur Durchführung biochemischer Analysen und Verfahren zu ihrer Herstellung
SE0103084D0 (sv) * 2001-09-14 2001-09-14 Amersham Pharm Biotech Ab Generation of ion exchange media
GB0127809D0 (en) * 2001-11-20 2002-01-09 Dna Res Innovations Ltd Purification of nucleic acid
EP1468116A2 (de) 2002-01-16 2004-10-20 Dynal Biotech ASA Verfahren zur isolierung von nukleinsäuren und proteinen aus einer einzigen probe
AU2003217837A1 (en) * 2002-02-28 2003-09-16 Keck Graduate Institute Cationic polyelectrolytes in biomolecule purification and analysis
US20060035224A1 (en) * 2002-03-21 2006-02-16 Johansen Jack T Purification methods for oligonucleotides and their analogs
GB0207975D0 (en) * 2002-04-05 2002-05-15 Genovision As Isolating nucleic acid
GB0212826D0 (en) 2002-05-31 2002-07-10 Dna Res Innovations Ltd Materials and methods relating to polyions and substance delivery
US6989442B2 (en) * 2002-07-12 2006-01-24 Sirna Therapeutics, Inc. Deprotection and purification of oligonucleotides and their derivatives
DE602004018616D1 (de) * 2003-03-05 2009-02-05 Ge Healthcare Bio Sciences Ab Verfahren zur herstellung von multimodalen anionenaustauschliganden
SE0302911D0 (sv) * 2003-10-31 2003-10-31 Amersham Biosciences Ab Novel separation matrix
US20050106589A1 (en) * 2003-11-17 2005-05-19 Hashem Akhavan-Tafti Compositions and methods for releasing nucleic acids from solid phase binding materials
SE0303532D0 (sv) * 2003-12-23 2003-12-23 Amersham Biosciences Ab Purification of immunoglobulins
US7479222B2 (en) * 2004-02-05 2009-01-20 Millipore Corporation Porous adsorptive or chromatographic media
EP1693108A1 (de) 2004-12-04 2006-08-23 MERCK PATENT GmbH Mischmodus Anionenaustauschertrennungsmaterial
KR100668337B1 (ko) 2005-05-21 2007-01-12 삼성전자주식회사 단백질에 비하여 핵산에 대한 결합력이 선택적으로 높은ｐＨ 의존성 이온 교환물질, 그가 고정화되어 있는 고체기판, 및 상기 물질 및 고체 기판을 이용하여 핵산을분리하는 방법
EP1754534A1 (de) 2005-08-03 2007-02-21 MERCK PATENT GmbH Hydrophiles vernetztes Polymer
CA2617645C (en) 2005-08-03 2014-09-09 Merck Patent Gesellschaft Mit Beschraenkter Haftung Hydrophilic crosslinked polymer
GB2443505B (en) * 2006-09-26 2008-12-31 Ge Healthcare Bio Sciences Nucleic acid purification method
GB2445441B (en) 2006-09-26 2010-06-30 Ge Healthcare Bio Sciences Nucleic acid purification method
GB2445442A (en) 2006-09-26 2008-07-09 Ge Healthcare Bio Sciences Nucleic acid purification using anion exchange
US9433922B2 (en) 2007-08-14 2016-09-06 Emd Millipore Corporation Media for membrane ion exchange chromatography based on polymeric primary amines, sorption device containing that media, and chromatography scheme and purification method using the same
DE202008017854U1 (de) 2007-08-14 2010-08-19 Millipore Corp., Billerica Mittel zur Ionenaustausch-Membranchromatographie auf der Basis von primären polymerischen Aminen, Sorptionsvorrichtung mit diesem Mittel, Chromatographieverfahren und Reinigungsverfahren unter dessen Verwendung
US20090130738A1 (en) * 2007-11-19 2009-05-21 Mikhail Kozlov Media for membrane ion exchange chromatography
EP2250182B1 (de) * 2008-02-07 2015-12-02 GE Healthcare Bio-Sciences Corp. Isolierung von dna, rna und protein aus einer einzelnen probe
JP5999899B2 (ja) 2008-04-08 2016-09-28 バイオ−ラッド ラボラトリーズ インコーポレーティッド 抗体のクロマトグラフィー精製法
WO2009145722A1 (en) * 2008-05-30 2009-12-03 Ge Healthcare Bio-Sciences Ab Separation method utilizing polyallylamine ligands
JP6090985B2 (ja) * 2011-01-12 2017-03-08 積水メディカル株式会社 核酸鎖の分離方法
US20140147842A1 (en) 2011-01-12 2014-05-29 Sekisui Medical Co. Ltd Method for detecting single nucleotide polymorphisms
JP5734320B2 (ja) * 2011-02-10 2015-06-17 積水メディカル株式会社 イオン交換クロマトグラフィー用充填剤及び核酸鎖の分離検出方法
EP2822687B1 (de) * 2012-03-08 2019-11-27 Bio-rad Laboratories, Inc. Anionischer austausch-hydrophober mischmodus
US9169304B2 (en) 2012-05-01 2015-10-27 Pfenex Inc. Process for purifying recombinant Plasmodium falciparum circumsporozoite protein
MY178616A (en) 2012-09-17 2020-10-19 Grace W R & Co Chromatography media and devices
MY195756A (en) * 2012-09-17 2023-02-09 Grace W R & Co Functionalized Particulate Support Material and Methods of Making and Using the Same
EP2925432A4 (de) * 2012-11-29 2016-08-31 Affigel Ab Superhydroxylierte absorptionsmittel und verwendungen davon
JP6853670B2 (ja) 2014-01-16 2021-04-07 ダブリュー・アール・グレース・アンド・カンパニー−コーンＷ Ｒ Ｇｒａｃｅ ＆ Ｃｏ−Ｃｏｎｎ 親和性クロマトグラフィー媒体及びクロマトグラフィーデバイス
US11285460B2 (en) 2014-03-14 2022-03-29 Cytiva Bioprocess R&D Ab Separation matrices for purification of biological particles
CN107847907A (zh) 2014-05-02 2018-03-27 格雷斯公司 官能化载体材料以及制备和使用官能化载体材料的方法
EP3149022A4 (de) 2014-05-28 2018-01-10 Agency for Science, Technology and Research Virusreduktionsverfahren
US10526287B2 (en) 2015-04-23 2020-01-07 Constellation Pharmaceuticals, Inc. LSD1 inhibitors and uses thereof
EP3302784B1 (de) 2015-06-05 2021-10-06 W.R. Grace & Co.-Conn. Adsorbierende klärmittel für bioprozesstechnik und verfahren zur herstellung und verwendung davon
US11059856B2 (en) * 2016-09-15 2021-07-13 Klawego Gmbh & Co. Kg Use of a polymeric mesh for the purification of macromolecules
WO2018081343A1 (en) 2016-10-26 2018-05-03 Constellation Pharmaceuticals, Inc. Lsd1 inhibitors and medical uses thereof
JP7045373B2 (ja) * 2017-06-22 2022-03-31 昭和電工株式会社 オリゴヌクレオチドの混合物の分離分析方法
WO2019152977A2 (en) 2018-02-05 2019-08-08 Bio-Rad Laboratories, Inc. Chromatography resin having an anionic exchange-hydrophobic mixed mode ligand
EP4025336A4 (de) 2019-09-05 2024-01-17 Bio Rad Laboratories Inc Anionenaustausch-/hydrophobe harze für mischmoduschromatografie
US11912983B2 (en) 2019-12-05 2024-02-27 Waters Technologies Corporation Polyanionic acids to improve recovery and minimize system loss
EP4182460A1 (de) * 2020-07-14 2023-05-24 Waters Technologies Corporation Kits und verfahren zur trennung von oligonukleotiden von matrixkomponenten

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4767670A (en) * 1987-01-21 1988-08-30 E. I. Du Pont De Nemours And Company Chromatographic supports for separation of oligonucleotides
SE8902315D0 (sv) * 1989-06-27 1989-06-27 Pharmacia Ab Anjonbytare
US5087359A (en) * 1989-08-07 1992-02-11 J. T. Baker Inc. Quaternized PEI silica solid supports for chromatography
EP0416748B1 (de) * 1989-08-07 1994-03-30 J.T. Baker Inc. Feste Träger aus quaternisierter PEI-Kieselsäure für die Chromatographie
SE9004129D0 (sv) * 1990-12-21 1990-12-21 Pharmacia Lkb Biotech Anion exchanger

Also Published As

Publication number Publication date
EP0888157A1 (de) 1999-01-07
JP2000504625A (ja) 2000-04-18
WO1997029825A1 (en) 1997-08-21
ATE267037T1 (de) 2004-06-15
AU1818097A (en) 1997-09-02
SE9600590D0 (sv) 1996-02-19
DE69729182T2 (de) 2005-01-27
AU713635B2 (en) 1999-12-09
US6090288A (en) 2000-07-18
JP4422797B2 (ja) 2010-02-24
EP0888157B1 (de) 2004-05-19

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Owner name: GE HEALTHCARE BIO-SCIENCES AB, UPPSALA, SE

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Representative=s name: HAMMONDS LLP, LONDON, GB

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