AU660161B2 - A method of constructing synthetic leader sequences - Google Patents

A method of constructing synthetic leader sequences

Info

Publication number
AU660161B2
AU660161B2 AU91348/91A AU9134891A AU660161B2 AU 660161 B2 AU660161 B2 AU 660161B2 AU 91348/91 A AU91348/91 A AU 91348/91A AU 9134891 A AU9134891 A AU 9134891A AU 660161 B2 AU660161 B2 AU 660161B2
Authority
AU
Australia
Prior art keywords
sequence encoding
dna sequence
yeast
arg
signal peptide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
AU91348/91A
Other languages
English (en)
Other versions
AU9134891A (en
Inventor
Lars Christiansen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novo Nordisk AS
Original Assignee
Novo Nordisk AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novo Nordisk AS filed Critical Novo Nordisk AS
Publication of AU9134891A publication Critical patent/AU9134891A/en
Application granted granted Critical
Publication of AU660161B2 publication Critical patent/AU660161B2/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Mycology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Consolidation Of Soil By Introduction Of Solidifying Substances Into Soil (AREA)
AU91348/91A 1990-12-19 1991-12-18 A method of constructing synthetic leader sequences Ceased AU660161B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DK3000/90 1990-12-19
DK300090A DK300090D0 (da) 1990-12-19 1990-12-19 Fremgangsmaade til fremstilling af leadersekvenser
PCT/DK1991/000396 WO1992011378A1 (en) 1990-12-19 1991-12-18 A method of constructing synthetic leader sequences

Publications (2)

Publication Number Publication Date
AU9134891A AU9134891A (en) 1992-07-22
AU660161B2 true AU660161B2 (en) 1995-06-15

Family

ID=8118017

Family Applications (1)

Application Number Title Priority Date Filing Date
AU91348/91A Ceased AU660161B2 (en) 1990-12-19 1991-12-18 A method of constructing synthetic leader sequences

Country Status (17)

Country Link
EP (1) EP0563175A1 (de)
JP (1) JPH06503957A (de)
KR (1) KR930703450A (de)
AU (1) AU660161B2 (de)
CA (1) CA2098731A1 (de)
CZ (1) CZ119293A3 (de)
DK (1) DK300090D0 (de)
FI (1) FI932831A (de)
HU (1) HUT68751A (de)
IE (1) IE914433A1 (de)
IL (1) IL100408A0 (de)
MX (1) MX9102684A (de)
NZ (1) NZ241011A (de)
PT (1) PT99848A (de)
SK (1) SK62593A3 (de)
WO (1) WO1992011378A1 (de)
ZA (1) ZA919932B (de)

Families Citing this family (38)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FI92601C (fi) * 1992-03-11 1994-12-12 Marja Makarow Menetelmä hyötyproteiinien erittämiseksi hiivoista
US5538863A (en) * 1993-07-01 1996-07-23 Immunex Corporation Expression system comprising mutant yeast strain and expression vector encoding synthetic signal peptide
US5639642A (en) * 1994-06-16 1997-06-17 Novo Nordisk A/S Synthetic leader peptide sequences
ZA954983B (en) * 1994-06-17 1996-02-14 Novo Nordisk As N-terminally extended proteins expressed in yeast
US6500645B1 (en) 1994-06-17 2002-12-31 Novo Nordisk A/S N-terminally extended proteins expressed in yeast
HRP940432B1 (en) * 1994-08-05 2003-10-31 Pliva Pharm & Chem Works Dna sequences encoding biosynthetic insulin precursors and process for preparation of insulin
ATE315083T1 (de) 1995-03-17 2006-02-15 Novozymes As Neue endoglukanase
DE69740176D1 (de) 1996-03-01 2011-05-26 Novo Nordisk As Appetithemmendes Peptid, Zusammensetzung und Verwendung
JP4341859B2 (ja) 1996-12-13 2009-10-14 ノバルティス バクシンズ アンド ダイアグノスティックス， インコーポレーテッド 酵母での異種タンパク質の発現の方法
EP1049790A1 (de) 1998-01-23 2000-11-08 Novo Nordisk A/S Verfahren zur herstellung erwünschter polypeptide in hefe
RU2002120513A (ru) 1999-12-29 2004-03-27 Ново Нордиск А/С (DK) Способ производства предшественников инсулина и аналогов предшественников инсулина с повышенным выходом ферментации у дрожжей
AT410217B (de) * 2000-06-15 2003-03-25 Cistem Biotechnologies Gmbh Vektor und ein verfahren zur expression und selektion randomisierter peptid-sequenzen
IL157842A0 (en) 2001-03-22 2004-03-28 Novo Nordisk Healthcare Ag Coagulation factor vii derivatives
EP1432794B1 (de) 2001-09-27 2011-11-09 Novo Nordisk Health Care AG Menschliche gerinnungsfaktor-vii-polypeptide
ES2381110T3 (es) 2003-09-09 2012-05-23 Novo Nordisk Health Care Ag Polipéptidos de factor VII de coagulación
WO2005047508A1 (en) 2003-11-14 2005-05-26 Novo Nordisk A/S Processes for making acylated insulin
JP5697831B2 (ja) 2003-12-03 2015-04-08 ノヴォ ノルディスク アー／エス 単鎖インシュリン
WO2005078116A1 (fr) * 2004-01-16 2005-08-25 Qiuyun Liu Methode permettant d'isoler de peptides antibacteriens et peptides isoles
WO2006111524A2 (en) 2005-04-18 2006-10-26 Novo Nordisk A/S Il-21 variants
EP1917363B1 (de) 2005-08-16 2011-06-22 Novo Nordisk A/S Verfahren zur herstellung reifer insulinpolypeptide
EP1926817A2 (de) 2005-09-14 2008-06-04 Novo Nordisk Health Care AG Menschliche gerinnungsfaktor-vii-polypeptide
DE602007009496D1 (de) 2006-02-27 2010-11-11 Novo Nordisk As Insulinderivate
EP2069502B1 (de) 2006-09-27 2014-02-26 Novo Nordisk A/S Verfahren zur herstellung gereifter insulinpolypeptide
EP2178909B1 (de) 2007-08-13 2015-10-21 Novo Nordisk A/S Insulinanaloga mit schneller wirkung
JP5721432B2 (ja) 2007-08-15 2015-05-20 ノボ・ノルデイスク・エー／エス アミノ酸含有アルキレングリコール反復単位を含むアシル部を有するインスリン
EP2178912B1 (de) 2007-08-15 2015-07-08 Novo Nordisk A/S Insulinanaloge mit acyl- und alkylenglycolteilen
EP2406282A1 (de) 2009-03-11 2012-01-18 Novo Nordisk A/S Interleukin-21-varianten mit antagonistischer bindung an den il-21-rezeptor
WO2011006982A2 (en) 2009-07-17 2011-01-20 Rigshospitalet Inhibitors of complement activation
US8815541B2 (en) 2009-11-25 2014-08-26 Novo Nordisk A/S Method for making polypeptides
ES2583259T3 (es) 2009-12-01 2016-09-20 Novo Nordisk A/S Nuevas liasas alfa-amidantes de peptidil alfa-hidroxiglicina
CN102791730A (zh) 2010-01-22 2012-11-21 诺沃—诺迪斯克有限公司 低度o-糖基化的fgf21的制备方法
CA2791841C (en) 2010-03-05 2023-01-03 Rigshospitalet Chimeric inhibitor molecules of complement activation
JP2013535467A (ja) * 2010-07-28 2013-09-12 スマートセルズ・インコーポレイテツド 組換えにより発現されたインスリンポリペプチドおよびその使用
EP2812024B1 (de) 2012-02-09 2018-04-11 Var2 Pharmaceuticals ApS Targeting von chondroitinsulfatglycanen
EP2906693A1 (de) 2012-10-15 2015-08-19 Novo Nordisk Health Care AG Gerinnungsfaktor-vii-polypeptide
EP3004156A1 (de) 2013-06-07 2016-04-13 Novo Nordisk A/S Verfahren zur herstellung von reifen insulinpolypeptiden
US20160376330A1 (en) * 2014-02-28 2016-12-29 Novo Nordisk A/S Mating factor alpha pro-peptide variants
CN112789504A (zh) 2018-07-13 2021-05-11 瓦克特诊断有限责任公司 使用重组疟疾蛋白var2csa分离胎儿来源的循环细胞

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK463887D0 (da) * 1987-09-07 1987-09-07 Novo Industri As Gaerleader
CA1340772C (en) * 1987-12-30 1999-09-28 Patricia Tekamp-Olson Expression and secretion of heterologous protiens in yeast employing truncated alpha-factor leader sequences
WO1990001063A1 (en) * 1988-07-23 1990-02-08 Delta Biotechnology Limited New secretory leader sequences
DK105489D0 (da) * 1989-03-03 1989-03-03 Novo Nordisk As Polypeptid

Also Published As

Publication number Publication date
NZ241011A (en) 1993-04-28
FI932831A0 (fi) 1993-06-18
AU9134891A (en) 1992-07-22
IL100408A0 (en) 1992-09-06
MX9102684A (es) 1992-06-01
JPH06503957A (ja) 1994-05-12
EP0563175A1 (de) 1993-10-06
FI932831A (fi) 1993-06-18
HUT68751A (en) 1995-07-28
ZA919932B (en) 1992-08-26
KR930703450A (ko) 1993-11-30
HU9301801D0 (en) 1993-10-28
CA2098731A1 (en) 1992-06-19
SK62593A3 (en) 1993-10-06
WO1992011378A1 (en) 1992-07-09
DK300090D0 (da) 1990-12-19
CZ119293A3 (en) 1994-02-16
IE914433A1 (en) 1992-07-01
PT99848A (pt) 1993-06-30

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