AU6543286A - Method of using bar1 for secreting foreign proteins - Google Patents

Method of using bar1 for secreting foreign proteins

Info

Publication number
AU6543286A
AU6543286A AU65432/86A AU6543286A AU6543286A AU 6543286 A AU6543286 A AU 6543286A AU 65432/86 A AU65432/86 A AU 65432/86A AU 6543286 A AU6543286 A AU 6543286A AU 6543286 A AU6543286 A AU 6543286A
Authority
AU
Australia
Prior art keywords
barl
promoter
fragment
gene
plasmid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU65432/86A
Other languages
English (en)
Inventor
Vivian L. Mackay
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Zymogenetics Inc
Original Assignee
Zymogenetics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Zymogenetics Inc filed Critical Zymogenetics Inc
Publication of AU6543286A publication Critical patent/AU6543286A/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C12N9/60—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi from yeast
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/10—Fusion polypeptide containing a localisation/targetting motif containing a tag for extracellular membrane crossing, e.g. TAT or VP22
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Mycology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Diabetes (AREA)
  • Endocrinology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
AU65432/86A 1985-10-25 1986-10-20 Method of using bar1 for secreting foreign proteins Abandoned AU6543286A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US79130585A 1985-10-25 1985-10-25
US791305 1985-10-25

Related Child Applications (1)

Application Number Title Priority Date Filing Date
AU74003/91A Division AU676132B2 (en) 1985-10-25 1991-04-02 Method of using bar1 for secreting foreign proteins

Publications (1)

Publication Number Publication Date
AU6543286A true AU6543286A (en) 1987-05-19

Family

ID=25153298

Family Applications (2)

Application Number Title Priority Date Filing Date
AU65432/86A Abandoned AU6543286A (en) 1985-10-25 1986-10-20 Method of using bar1 for secreting foreign proteins
AU74003/91A Ceased AU676132B2 (en) 1985-10-25 1991-04-02 Method of using bar1 for secreting foreign proteins

Family Applications After (1)

Application Number Title Priority Date Filing Date
AU74003/91A Ceased AU676132B2 (en) 1985-10-25 1991-04-02 Method of using bar1 for secreting foreign proteins

Country Status (13)

Country Link
EP (1) EP0243465A1 (fr)
JP (1) JP2523562B2 (fr)
CN (1) CN1027179C (fr)
AU (2) AU6543286A (fr)
CA (1) CA1316133C (fr)
CZ (1) CZ284251B6 (fr)
DK (1) DK320287D0 (fr)
FI (1) FI872801A (fr)
HU (1) HU206897B (fr)
IE (1) IE63822B1 (fr)
SK (1) SK279041B6 (fr)
UA (1) UA41863C2 (fr)
WO (1) WO1987002670A1 (fr)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU590197B2 (en) * 1984-05-30 1989-11-02 Novo Nordisk A/S Insulin precursors, process for their preparation and process for preparing human insulin from such insulin precursors
AU633020B2 (en) * 1988-07-23 1993-01-21 Novozymes Delta Limited New secretory leader sequences
AU660172B2 (en) * 1987-10-02 1995-06-15 Zymogenetics Inc. BAR1 secretion signal

Families Citing this family (51)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5206699A (en) * 1988-05-06 1993-04-27 Gersan Establishment Sensing a narrow frequency band of radiation and gemstones
WO1991013971A1 (fr) * 1990-03-13 1991-09-19 Hawaii Biotechnology Group, Inc. SYSTEME D'EXPRESSION A $i(NEUROSPORA)
DK82893D0 (da) * 1993-07-08 1993-07-08 Novo Nordisk As Peptid
EP1683860B1 (fr) 1995-03-17 2013-10-23 Novozymes A/S Nouvelles endoglucanases
EP1975177B1 (fr) 1996-03-01 2011-04-13 Novo Nordisk A/S Peptide de suppression de l'appétit, ses compositions et son utilisation
AU782580B2 (en) 2000-01-10 2005-08-11 Maxygen, Inc. G-CSF conjugates
ATE428445T1 (de) 2000-02-11 2009-05-15 Bayer Healthcare Llc Gerinnungsfaktor vii oder viia konjugate
YU48703A (sh) 2001-02-27 2006-05-25 Maxygen Aps Novi interferonu beta-slični molekuli
EP1373493B1 (fr) 2001-03-22 2013-07-31 Novo Nordisk Health Care AG Derive du facteur vii de coagulation
US6534059B2 (en) 2001-06-05 2003-03-18 Advanced Biotherapy, Inc. Compositions and methods for treating hyperimmune response in the eye
ATE532858T1 (de) 2001-09-27 2011-11-15 Novo Nordisk Healthcare Ag Menschliche gerinnungsfaktor-vii-polypeptide
ES2381110T3 (es) 2003-09-09 2012-05-23 Novo Nordisk Health Care Ag Polipéptidos de factor VII de coagulación
BRPI0609079A2 (pt) 2005-04-18 2010-11-16 Novo Nordisk As peptìdeo, composição farmacêutica, método para o tratamento de cáncer, uso de um peptìdeo construto de ácido nucleico, vetor, hospedeiro, e, anticorpo
WO2007020256A1 (fr) 2005-08-16 2007-02-22 Novo Nordisk A/S Procede permettant de realiser des polypeptides d'insuline matures
JP5690047B2 (ja) 2005-09-14 2015-03-25 ノボ ノルディスク ヘルス ケア アーゲー ヒト凝固第ｖｉｉ因子ポリペプチド
EP2407561A3 (fr) 2006-03-06 2012-05-30 Humagene, Inc. Procédé de préparation de fibrinogène humaine recombinante
CN106008699A (zh) 2006-09-08 2016-10-12 Ambrx公司 经修饰的人类血浆多肽或Fc骨架和其用途
EP2069502B1 (fr) 2006-09-27 2014-02-26 Novo Nordisk A/S Procédé de fabrication de polypeptides d'insuline mature
AU2008247815B2 (en) 2007-05-02 2012-09-06 Ambrx, Inc. Modified interferon beta polypeptides and their uses
JP5547083B2 (ja) 2007-11-20 2014-07-09 アンブルックス，インコーポレイテッド 修飾されたインスリンポリペプチドおよびそれらの使用
US10138283B2 (en) 2008-07-23 2018-11-27 Ambrx, Inc. Modified bovine G-CSF polypeptides and their uses
US8349325B2 (en) 2008-12-23 2013-01-08 Abbott Laboratories Soluble FMS-like tyrosine kinase-1 (sFLT-1) antibody and related composition, kit, methods of using, and materials and method for making
US20120107267A1 (en) 2009-03-11 2012-05-03 Novo Nordisk A/S Interleukin-21 variants having antagonistic binding to the il-21 receptor
KR102184713B1 (ko) 2009-07-17 2020-12-01 오메로스 코포레이션 보체 활성화의 억제제로서의 masp 이소형
US9096843B2 (en) 2009-12-01 2015-08-04 Novo Nordisk A/S Peptidyl α-hydroxyglycine α-amidating lyases
CA2784800A1 (fr) 2009-12-21 2011-07-21 Ambrx, Inc. Polypeptides d'hormone de croissance porcine modifiee et leurs utilisations
AU2010341516B2 (en) 2009-12-21 2014-01-16 Ambrx, Inc. Modified bovine somatotropin polypeptides and their uses
AU2011222883B2 (en) 2010-03-05 2016-05-26 Omeros Corporation Chimeric inhibitor molecules of complement activation
US20110229921A1 (en) 2010-03-18 2011-09-22 Abbott Laboratories METHODS OF ASSAYING URINARY NEUTROPHIL GELATINASE-ASSOCIATED LIPOCALIN (uNGAL) IN THE PROGNOSIS OF CADAVERIC KIDNEY TRANSPLANT FUNCTION IN A PATIENT, INCLUDING A PATIENT DIAGNOSED WITH DELAYED GRAFT FUNCTION (DGF), A METHOD OF ASSAYING uNGAL IN THE ASSESSMENT OF RISK OF DGF IN A PATIENT DIAGNOSED WITH EARLY GRAFT FUNCTION (EGF), AND RELATED KITS
WO2011163558A1 (fr) 2010-06-25 2011-12-29 Abbott Laboratories Matériaux et procédés pour le dosage d'anticorps anti-virus de l'hépatite c (hvc)
SG10201506443TA (en) 2010-08-17 2015-10-29 Ambrx Inc Modified relaxin polypeptides and their uses
AR083006A1 (es) 2010-09-23 2013-01-23 Lilly Co Eli Formulaciones para el factor estimulante de colonias de granulocitos (g-csf) bovino y variantes de las mismas
US20120115244A1 (en) 2010-11-09 2012-05-10 Abbott Laboratories Materials and methods for immunoassay of pterins
CN105821078A (zh) 2010-12-09 2016-08-03 巴斯德研究所 用于获得高产量重组蛋白表达的基于mgmt的方法
AU2012350229B2 (en) 2011-12-09 2018-03-22 Institut Pasteur Multiplex immuno screening assay
US8993248B2 (en) 2011-12-31 2015-03-31 Abbott Laboratories Truncated human vitamin D binding protein and mutation and fusion thereof and related materials and methods of use
JP6517018B2 (ja) 2012-02-09 2019-05-22 ヴァー２・ファーマシューティカルズ・アンパルトセルスカブＶＡＲ２ Ｐｈａｒｍａｃｅｕｔｉｃａｌｓ ＡｐＳ コンドロイチン硫酸グリカンのターゲティング
EP2906693A1 (fr) 2012-10-15 2015-08-19 Novo Nordisk Health Care AG Polypeptides de facteur vii de coagulation
JP2014128262A (ja) * 2012-11-27 2014-07-10 Kirin Brewery Co Ltd 接合能を持つ酵母細胞株のスクリーニング方法
AU2019263303A1 (en) 2018-05-01 2020-12-24 Ambrx, Inc. A method for optimizing antibody expression
CN112789504A (zh) 2018-07-13 2021-05-11 瓦克特诊断有限责任公司 使用重组疟疾蛋白var2csa分离胎儿来源的循环细胞
CA3111576A1 (fr) 2018-09-11 2020-03-19 Ambrx, Inc. Conjugues polypeptidiques d'interleukine-2 et leurs utilisations
EP3867265A1 (fr) 2018-10-19 2021-08-25 Ambrx, Inc. Conjugués polypeptidiques d'interleukine-10, dimères de ceux-ci et leurs utilisations
CA3128081A1 (fr) 2019-02-12 2020-08-20 Ambrx, Inc. Contenant de compositions, procedes et utilisations de conjugues anticorps-agonistes tlr
KR20220151202A (ko) 2020-03-11 2022-11-14 암브룩스, 인코포레이티드 인터류킨-2 폴리펩타이드 접합체 및 그의 사용 방법
CA3175523A1 (fr) 2020-04-13 2021-10-21 Antti Virtanen Procedes, complexes et kits pour detecter ou determiner une quantite d'un anticorps a .beta.-coronavirus dans un echantillon
EP4193149A1 (fr) 2020-08-04 2023-06-14 Abbott Laboratories Méthodes et kits améliorés pour détecter une protéine sars-cov-2 dans un échantillon
US20230302150A1 (en) 2020-08-20 2023-09-28 Ambrx, Inc. Antibody-tlr agonist conjugates, methods and uses thereof
EP4271998A1 (fr) 2020-12-30 2023-11-08 Abbott Laboratories Procédés pour déterminer un antigène sras-cov-2 et anticorps anti-sras-cov-2 dans un échantillon
AU2022249223A1 (en) 2021-04-03 2023-10-12 Ambrx, Inc. Anti-her2 antibody-drug conjugates and uses thereof
CN115806889B (zh) * 2022-09-28 2024-06-11 山东大学 一种提高基因表达水平的酿酒酵母工程菌及其构建方法与应用

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4546082A (en) * 1982-06-17 1985-10-08 Regents Of The Univ. Of California E. coli/Saccharomyces cerevisiae plasmid cloning vector containing the alpha-factor gene for secretion and processing of hybrid proteins
US4613572A (en) * 1983-08-15 1986-09-23 Kansas State University Research Foundation Yeast BAR1 gene plasmid
DE3537176C2 (de) * 1984-10-18 1994-06-09 Zymogenetics Inc Gewebeplasminogenaktivator und Verfahren zu seiner Herstellung
JPS61247384A (ja) * 1984-10-18 1986-11-04 チモ−ジエネテイツクス　インコ−ポレ−テツド 酵母中でプラスミノ−ゲン活性化因子を発現する方法

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU590197B2 (en) * 1984-05-30 1989-11-02 Novo Nordisk A/S Insulin precursors, process for their preparation and process for preparing human insulin from such insulin precursors
AU660172B2 (en) * 1987-10-02 1995-06-15 Zymogenetics Inc. BAR1 secretion signal
AU633020B2 (en) * 1988-07-23 1993-01-21 Novozymes Delta Limited New secretory leader sequences

Also Published As

Publication number Publication date
AU676132B2 (en) 1997-03-06
JP2523562B2 (ja) 1996-08-14
HU206897B (en) 1993-01-28
CN86107554A (zh) 1987-08-26
WO1987002670A1 (fr) 1987-05-07
CZ284251B6 (cs) 1998-10-14
FI872801A0 (fi) 1987-06-24
IE862804L (en) 1987-04-25
IE63822B1 (en) 1995-06-14
SK776486A3 (en) 1998-06-03
CA1316133C (fr) 1993-04-13
UA41863C2 (uk) 2001-10-15
JPS63501614A (ja) 1988-06-23
HUT43624A (en) 1987-11-30
DK320287A (da) 1987-06-23
CZ776486A3 (en) 1996-09-11
EP0243465A1 (fr) 1987-11-04
CN1027179C (zh) 1994-12-28
SK279041B6 (sk) 1998-06-03
AU7400391A (en) 1991-07-18
FI872801A (fi) 1987-06-24
DK320287D0 (da) 1987-06-23

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