AU2016432443B2 - Thermostable Cas9 nucleases - Google Patents

Thermostable Cas9 nucleases Download PDF

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AU2016432443B2
AU2016432443B2 AU2016432443A AU2016432443A AU2016432443B2 AU 2016432443 B2 AU2016432443 B2 AU 2016432443B2 AU 2016432443 A AU2016432443 A AU 2016432443A AU 2016432443 A AU2016432443 A AU 2016432443A AU 2016432443 B2 AU2016432443 B2 AU 2016432443B2
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arg
glu
sequence
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AU2016432443A1 (en
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Elleke Fenna BOSMA
Ioannis MOUGIAKOS
John Van Der Oost
Richard Van Kranenburg
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Stichting voor de Technische Wetenschappen STW
Wageningen Universiteit
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STICHTING TECHNISCHE WETENSCHAPPEN
Stichting voor de Technische Wetenschappen STW
Wageningen Universiteit
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
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    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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    • C12N2310/00—Structure or type of the nucleic acid
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    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/50—Physical structure
    • C12N2310/53—Physical structure partially self-complementary or closed
    • C12N2310/531—Stem-loop; Hairpin
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    • C12N2330/50—Biochemical production, i.e. in a transformed host cell
    • C12N2330/51—Specially adapted vectors
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    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14141—Use of virus, viral particle or viral elements as a vector
    • C12N2750/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector

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AU2016432443A 2016-12-14 2016-12-14 Thermostable Cas9 nucleases Active AU2016432443B2 (en)

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PCT/EP2016/081077 WO2018108272A1 (en) 2016-12-14 2016-12-14 Thermostable cas9 nucleases

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AU2016432443A1 AU2016432443A1 (en) 2019-07-11
AU2016432443B2 true AU2016432443B2 (en) 2024-04-18

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AU2016432443A Active AU2016432443B2 (en) 2016-12-14 2016-12-14 Thermostable Cas9 nucleases
AU2017377135A Active AU2017377135B2 (en) 2016-12-14 2017-08-16 Thermostable Cas9 nucleases
AU2017377136A Active AU2017377136B2 (en) 2016-12-14 2017-08-16 Thermostable Cas9 nucleases
AU2017377792A Active AU2017377792B2 (en) 2016-12-14 2017-12-14 Thermostable Cas9 nucleases

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AU2017377136A Active AU2017377136B2 (en) 2016-12-14 2017-08-16 Thermostable Cas9 nucleases
AU2017377792A Active AU2017377792B2 (en) 2016-12-14 2017-12-14 Thermostable Cas9 nucleases

Country Status (11)

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US (5) US11242513B2 (OSRAM)
EP (4) EP3555275A1 (OSRAM)
JP (6) JP7182545B2 (OSRAM)
KR (4) KR20190104342A (OSRAM)
CN (4) CN110312792B (OSRAM)
AU (4) AU2016432443B2 (OSRAM)
BR (3) BR112019012155A2 (OSRAM)
CA (4) CA3046824A1 (OSRAM)
EA (4) EA201991443A1 (OSRAM)
PH (4) PH12019501335A1 (OSRAM)
WO (3) WO2018108272A1 (OSRAM)

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CN114480347B (zh) * 2022-02-21 2022-12-23 中国科学院地球化学研究所 一种纯化Cas12a蛋白的方法
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