AU2014293075A1 - Methods and compositions for detecting bacterial contamination - Google Patents
Methods and compositions for detecting bacterial contamination Download PDFInfo
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- AU2014293075A1 AU2014293075A1 AU2014293075A AU2014293075A AU2014293075A1 AU 2014293075 A1 AU2014293075 A1 AU 2014293075A1 AU 2014293075 A AU2014293075 A AU 2014293075A AU 2014293075 A AU2014293075 A AU 2014293075A AU 2014293075 A1 AU2014293075 A1 AU 2014293075A1
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Families Citing this family (25)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2015013465A2 (en) | 2013-07-25 | 2015-01-29 | Dch Molecular Diagnostics, Inc. | Methods and compositions for detecting bacterial contamination |
| EP3543362B1 (en) * | 2013-10-07 | 2021-09-08 | Mitsui Chemicals, Inc. | Pcr primer set for bacterial dna amplification, kit for detecting and/or identifying bacterial species, and method for detecting and/or identifying bacterial species |
| US9953415B2 (en) * | 2014-12-03 | 2018-04-24 | Tata Consultancy Services Limited | System and method for quantification of Escherichia coli bacteria in water |
| WO2017027835A1 (en) * | 2015-08-12 | 2017-02-16 | Circulogene Theranostics, Llc | Method of preparing cell free nucleic acid molecules by in situ amplification |
| CN105368961A (zh) * | 2015-12-15 | 2016-03-02 | 天津脉络生物科技有限公司 | 一种沙雷菌定量检测荧光pcr试剂盒及其应用 |
| CA3185611A1 (en) * | 2016-03-25 | 2017-09-28 | Karius, Inc. | Synthetic nucleic acid spike-ins |
| CN105734164B (zh) * | 2016-05-06 | 2019-05-17 | 苏州大学 | 一种检测细菌性脑膜炎病原体的多重pcr试剂盒 |
| CN111073987B (zh) * | 2016-08-30 | 2022-09-20 | 上海市生物医药技术研究院 | 小肠结肠炎耶尔森氏菌的快速恒温检测方法、引物组及试剂盒 |
| US20190062809A1 (en) * | 2017-08-24 | 2019-02-28 | Clinical Micro Sensors, Inc. (dba GenMark Diagnostics, Inc.) | Electrochemical detection of bacterial and/or fungal infections |
| CN111801429A (zh) * | 2017-11-06 | 2020-10-20 | 密歇根大学董事会 | 用于检测微生物的组合物和方法 |
| CN111527216A (zh) * | 2017-11-13 | 2020-08-11 | 生命技术公司 | 用于尿路微生物检测的组合物、方法和试剂盒 |
| EP3746565B1 (en) * | 2018-01-30 | 2025-12-03 | BioFire Defense, LLC | Methods and systems for validation of a nucleic acid amplification assay |
| CN108148834B (zh) * | 2018-03-14 | 2018-11-13 | 北京理工大学 | 用于检测空间环境中的真菌的引物组合、试剂盒及方法 |
| US20210172004A1 (en) * | 2018-04-13 | 2021-06-10 | National Agricultural Genotyping Center | Universal lactic acid bacteria quantification kit for fermentation monitoring |
| WO2020068881A1 (en) * | 2018-09-24 | 2020-04-02 | Second Genome, Inc. | Compositions, systems, apparatuses, and methods for validation of microbiome sequence processing and differential abundance analyses via multiple bespoke spike-in mixtures |
| CN109337967A (zh) * | 2018-09-27 | 2019-02-15 | 华中科技大学鄂州工业技术研究院 | 一种实验室的微生物污染鉴别方法 |
| FR3097560B1 (fr) * | 2019-06-24 | 2021-06-25 | Oreal | Méthode de diagnostic d’une exposition chronique à la pollution |
| CN111209895B (zh) * | 2020-02-14 | 2022-03-25 | 李淑媛 | 静脉药物配制样品的细菌污染检测系统、方法及存储介质 |
| CN112575097B (zh) * | 2020-10-12 | 2022-05-10 | 南开大学 | 一种用于检测蜡样芽孢杆菌的液相芯片及应用 |
| WO2022081815A1 (en) * | 2020-10-14 | 2022-04-21 | Pickens Peter Vincent | Optimized detection of blood-borne microbes |
| JP7553323B2 (ja) | 2020-10-30 | 2024-09-18 | 株式会社Kri | スタフィロコッカス細菌の同定方法 |
| CN112226528B (zh) * | 2020-12-14 | 2021-03-16 | 臻和(北京)生物科技有限公司 | 一种用于生物组织样本检测细菌污染的质检方法 |
| KR20240107097A (ko) * | 2021-09-03 | 2024-07-08 | 마이크로노마, 인크. | 암 관련 미생물 바이오마커를 식별하는 방법 |
| CN114480596A (zh) * | 2022-02-11 | 2022-05-13 | 宁波市中心血站 | 一种微流控血液及血制品细菌污染检测方法 |
| US12529106B2 (en) | 2023-06-09 | 2026-01-20 | Inti Taiwan, Inc. | Assays and methods to assist in vitro fertilization |
Family Cites Families (42)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5310652A (en) | 1986-08-22 | 1994-05-10 | Hoffman-La Roche Inc. | Reverse transcription with thermostable DNA polymerase-high temperature reverse transcription |
| US5322770A (en) | 1989-12-22 | 1994-06-21 | Hoffman-Laroche Inc. | Reverse transcription with thermostable DNA polymerases - high temperature reverse transcription |
| CA1340807C (en) | 1988-02-24 | 1999-11-02 | Lawrence T. Malek | Nucleic acid amplification process |
| US5130238A (en) | 1988-06-24 | 1992-07-14 | Cangene Corporation | Enhanced nucleic acid amplification process |
| US5234809A (en) | 1989-03-23 | 1993-08-10 | Akzo N.V. | Process for isolating nucleic acid |
| CA2020958C (en) | 1989-07-11 | 2005-01-11 | Daniel L. Kacian | Nucleic acid sequence amplification methods |
| FI911152A7 (fi) | 1989-07-11 | 1991-03-07 | Gen Probe Inc | Nukleiinihapposekvenssin amplifikaatiomenetelmät |
| US5494810A (en) | 1990-05-03 | 1996-02-27 | Cornell Research Foundation, Inc. | Thermostable ligase-mediated DNA amplifications system for the detection of genetic disease |
| US5210015A (en) | 1990-08-06 | 1993-05-11 | Hoffman-La Roche Inc. | Homogeneous assay system using the nuclease activity of a nucleic acid polymerase |
| US5455166A (en) | 1991-01-31 | 1995-10-03 | Becton, Dickinson And Company | Strand displacement amplification |
| US5994056A (en) | 1991-05-02 | 1999-11-30 | Roche Molecular Systems, Inc. | Homogeneous methods for nucleic acid amplification and detection |
| US5270184A (en) | 1991-11-19 | 1993-12-14 | Becton, Dickinson And Company | Nucleic acid target generation |
| US5925517A (en) | 1993-11-12 | 1999-07-20 | The Public Health Research Institute Of The City Of New York, Inc. | Detectably labeled dual conformation oligonucleotide probes, assays and kits |
| JP4071277B2 (ja) | 1993-11-12 | 2008-04-02 | ピーエイチアールアイ・プロパティーズ・インコーポレーテッド | 核酸検出用ハイブリダイゼーションプローブ、共通ステム、方法およびキット |
| US5538848A (en) | 1994-11-16 | 1996-07-23 | Applied Biosystems Division, Perkin-Elmer Corp. | Method for detecting nucleic acid amplification using self-quenching fluorescence probe |
| US5648211A (en) | 1994-04-18 | 1997-07-15 | Becton, Dickinson And Company | Strand displacement amplification using thermophilic enzymes |
| CA2150986C (en) | 1994-06-17 | 1999-11-02 | Mary Kathryn Meyer | Oligonucleotide primers and probes for detection of bacteria |
| US5641658A (en) | 1994-08-03 | 1997-06-24 | Mosaic Technologies, Inc. | Method for performing amplification of nucleic acid with two primers bound to a single solid support |
| US5705628A (en) | 1994-09-20 | 1998-01-06 | Whitehead Institute For Biomedical Research | DNA purification and isolation using magnetic particles |
| US5710029A (en) | 1995-06-07 | 1998-01-20 | Gen-Probe Incorporated | Methods for determining pre-amplification levels of a nucleic acid target sequence from post-amplification levels of product |
| US5705365A (en) | 1995-06-07 | 1998-01-06 | Gen-Probe Incorporated | Kits for determining pre-amplification levels of a nucleic acid target sequence from post-amplification levels of product |
| US5854033A (en) | 1995-11-21 | 1998-12-29 | Yale University | Rolling circle replication reporter systems |
| EP0892808B1 (en) | 1996-04-12 | 2008-05-14 | PHRI Properties, Inc. | Detection probes, kits and assays |
| US20100267012A1 (en) * | 1997-11-04 | 2010-10-21 | Bergeron Michel G | Highly conserved genes and their use to generate probes and primers for detection of microorganisms |
| JP2002503954A (ja) | 1997-04-01 | 2002-02-05 | グラクソ、グループ、リミテッド | 核酸増幅法 |
| WO1999011813A2 (en) | 1997-09-04 | 1999-03-11 | Bayer Corporation | Oligonucleotide probes bearing quenchable fluorescent labels, and methods of use thereof |
| AR021833A1 (es) | 1998-09-30 | 2002-08-07 | Applied Research Systems | Metodos de amplificacion y secuenciacion de acido nucleico |
| ATE374833T1 (de) | 1998-11-09 | 2007-10-15 | Eiken Chemical | Verfahren zur synthese von nukleinsäuren |
| MXPA02002656A (es) | 1999-09-13 | 2003-10-14 | Nugen Technologies Inc | Metodos y composiciones para la ampliacion lineal isotermica de secuencias de polinucleotidos. |
| EP1246935B1 (en) | 1999-09-28 | 2013-08-14 | Geneohm Sciences Canada Inc. | Highly conserved genes and their use to generate probes and primers for detection of microorganisms |
| US7019129B1 (en) | 2000-05-09 | 2006-03-28 | Biosearch Technologies, Inc. | Dark quenchers for donor-acceptor energy transfer |
| AUPQ909000A0 (en) * | 2000-07-28 | 2000-08-24 | University Of Sydney, The | A method of detecting microorganisms |
| US7001724B1 (en) | 2000-11-28 | 2006-02-21 | Applera Corporation | Compositions, methods, and kits for isolating nucleic acids using surfactants and proteases |
| JP4632788B2 (ja) | 2002-09-20 | 2011-02-16 | ニュー・イングランド・バイオラブズ・インコーポレイティッド | 核酸のヘリカーゼ依存性増幅 |
| AU2003288018A1 (en) | 2002-11-20 | 2004-06-15 | Pharmacia Italia S.P.A. | Method for the detection of microorganisms in pharmaceutical products |
| US20060160153A1 (en) * | 2002-12-03 | 2006-07-20 | Jean-Pierre Abastado | Method to measure a t cell response and its uses to qualify antigen-presenting cells |
| EP1473370A3 (en) * | 2003-04-24 | 2005-03-09 | BioMerieux, Inc. | Genus, group, species and/or strain specific 16S rDNA Sequences |
| CA2562221A1 (en) * | 2005-10-21 | 2007-04-21 | Canadian Blood Services | A method for detection of staphylococcus epidermidis |
| WO2008129428A2 (en) | 2007-04-19 | 2008-10-30 | Molecular Detection Inc. | Methods, compositions and kits for detection and analysis of antibiotic-resistant bacteria |
| US20130157265A1 (en) * | 2009-10-22 | 2013-06-20 | Jesus Mingorance Cruz | Composition, method and kit for detecting bacteria by means of sequencing |
| WO2012071135A2 (en) | 2010-11-23 | 2012-05-31 | Biovec Transfusion, Llc | Methods for removing pathogens from a platelet preparation |
| WO2015013465A2 (en) | 2013-07-25 | 2015-01-29 | Dch Molecular Diagnostics, Inc. | Methods and compositions for detecting bacterial contamination |
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| JP2022023068A (ja) | 2022-02-07 |
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| TW201525145A (zh) | 2015-07-01 |
| CN110592244A (zh) | 2019-12-20 |
| US20200080134A1 (en) | 2020-03-12 |
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| CN105473737A (zh) | 2016-04-06 |
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