CA2917430C - Methods and compositions for detecting bacterial contamination - Google Patents

Methods and compositions for detecting bacterial contamination Download PDF

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Publication number
CA2917430C
CA2917430C CA2917430A CA2917430A CA2917430C CA 2917430 C CA2917430 C CA 2917430C CA 2917430 A CA2917430 A CA 2917430A CA 2917430 A CA2917430 A CA 2917430A CA 2917430 C CA2917430 C CA 2917430C
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Prior art keywords
seq
primer
sequence
amplification
sample
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CA2917430A
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English (en)
French (fr)
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CA2917430A1 (en
Inventor
James Jian Quan Wang
Xiangmin Cui
Hiunam CHAN
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DCH MOLECULAR DIAGNOSTICS Inc
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DCH MOLECULAR DIAGNOSTICS Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/15—Modifications characterised by incorporating a consensus or conserved sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2531/00—Reactions of nucleic acids characterised by
    • C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/113—PCR

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CA2917430A 2013-07-25 2014-07-23 Methods and compositions for detecting bacterial contamination Active CA2917430C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361858495P 2013-07-25 2013-07-25
US61/858,495 2013-07-25
PCT/US2014/047914 WO2015013465A2 (en) 2013-07-25 2014-07-23 Methods and compositions for detecting bacterial contamination

Publications (2)

Publication Number Publication Date
CA2917430A1 CA2917430A1 (en) 2015-01-29
CA2917430C true CA2917430C (en) 2020-10-06

Family

ID=52393976

Family Applications (1)

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CA2917430A Active CA2917430C (en) 2013-07-25 2014-07-23 Methods and compositions for detecting bacterial contamination

Country Status (11)

Country Link
US (2) US10392670B2 (https=)
EP (1) EP3024947B1 (https=)
JP (3) JP2016525359A (https=)
KR (1) KR102245110B1 (https=)
CN (2) CN105473737B (https=)
AU (1) AU2014293075A1 (https=)
CA (1) CA2917430C (https=)
MX (1) MX2016000997A (https=)
SG (1) SG11201600550WA (https=)
TW (1) TWI697560B (https=)
WO (1) WO2015013465A2 (https=)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015013465A2 (en) 2013-07-25 2015-01-29 Dch Molecular Diagnostics, Inc. Methods and compositions for detecting bacterial contamination
MY195854A (en) * 2013-10-07 2023-02-23 Mitsui Chemicals Inc Primer Set for Pcr for Bacterial Dna Amplification, Kit For Detection and/or Identification of Bacterial Species, and, Method of Detection and/or Identification of Bacterial Species
US9953415B2 (en) * 2014-12-03 2018-04-24 Tata Consultancy Services Limited System and method for quantification of Escherichia coli bacteria in water
EP3757229A1 (en) * 2015-08-12 2020-12-30 Circulogene Theranostics, LLC Method of preparing cell free nucleic acid molecules by in situ amplification
CN105368961A (zh) * 2015-12-15 2016-03-02 天津脉络生物科技有限公司 一种沙雷菌定量检测荧光pcr试剂盒及其应用
EP3978627A1 (en) * 2016-03-25 2022-04-06 Karius, Inc. Methods using synthetic nucleic acid spike-ins
CN105734164B (zh) * 2016-05-06 2019-05-17 苏州大学 一种检测细菌性脑膜炎病原体的多重pcr试剂盒
CN111073987B (zh) * 2016-08-30 2022-09-20 上海市生物医药技术研究院 小肠结肠炎耶尔森氏菌的快速恒温检测方法、引物组及试剂盒
US20190062809A1 (en) * 2017-08-24 2019-02-28 Clinical Micro Sensors, Inc. (dba GenMark Diagnostics, Inc.) Electrochemical detection of bacterial and/or fungal infections
WO2019090306A1 (en) * 2017-11-06 2019-05-09 The Regents Of The University Of Michigan Compositions and methods for detection of microorganisms
WO2019094973A1 (en) * 2017-11-13 2019-05-16 Life Technologies Corporation Compositions, methods and kits for urinary tract microorganism detection
CN112105747A (zh) * 2018-01-30 2020-12-18 拜奥法尔防护有限责任公司 用于验证核酸扩增测定的方法和系统
CN108148834B (zh) * 2018-03-14 2018-11-13 北京理工大学 用于检测空间环境中的真菌的引物组合、试剂盒及方法
US20210172004A1 (en) * 2018-04-13 2021-06-10 National Agricultural Genotyping Center Universal lactic acid bacteria quantification kit for fermentation monitoring
US11473133B2 (en) * 2018-09-24 2022-10-18 Second Genome, Inc. Methods for validation of microbiome sequence processing and differential abundance analyses via multiple bespoke spike-in mixtures
CN109337967A (zh) * 2018-09-27 2019-02-15 华中科技大学鄂州工业技术研究院 一种实验室的微生物污染鉴别方法
FR3097560B1 (fr) * 2019-06-24 2021-06-25 Oreal Méthode de diagnostic d’une exposition chronique à la pollution
CN111209895B (zh) * 2020-02-14 2022-03-25 李淑媛 静脉药物配制样品的细菌污染检测系统、方法及存储介质
CN112575097B (zh) * 2020-10-12 2022-05-10 南开大学 一种用于检测蜡样芽孢杆菌的液相芯片及应用
WO2022081815A1 (en) * 2020-10-14 2022-04-21 Pickens Peter Vincent Optimized detection of blood-borne microbes
JP7553323B2 (ja) 2020-10-30 2024-09-18 株式会社Kri スタフィロコッカス細菌の同定方法
CN112226528B (zh) * 2020-12-14 2021-03-16 臻和(北京)生物科技有限公司 一种用于生物组织样本检测细菌污染的质检方法
MX2024002592A (es) * 2021-09-03 2024-06-11 Micronoma Inc Métodos para identificar biomarcadores microbianos asociados con cáncer.
CN114480596A (zh) * 2022-02-11 2022-05-13 宁波市中心血站 一种微流控血液及血制品细菌污染检测方法
US12529106B2 (en) 2023-06-09 2026-01-20 Inti Taiwan, Inc. Assays and methods to assist in vitro fertilization

Family Cites Families (42)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5310652A (en) 1986-08-22 1994-05-10 Hoffman-La Roche Inc. Reverse transcription with thermostable DNA polymerase-high temperature reverse transcription
US5322770A (en) 1989-12-22 1994-06-21 Hoffman-Laroche Inc. Reverse transcription with thermostable DNA polymerases - high temperature reverse transcription
CA1340807C (en) 1988-02-24 1999-11-02 Lawrence T. Malek Nucleic acid amplification process
US5130238A (en) 1988-06-24 1992-07-14 Cangene Corporation Enhanced nucleic acid amplification process
US5234809A (en) 1989-03-23 1993-08-10 Akzo N.V. Process for isolating nucleic acid
WO1991001384A1 (en) 1989-07-11 1991-02-07 Gen-Probe, Incorporated Nucleic acid sequence amplification methods utilizing a transcription complex
CA2020958C (en) 1989-07-11 2005-01-11 Daniel L. Kacian Nucleic acid sequence amplification methods
US5494810A (en) 1990-05-03 1996-02-27 Cornell Research Foundation, Inc. Thermostable ligase-mediated DNA amplifications system for the detection of genetic disease
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5455166A (en) 1991-01-31 1995-10-03 Becton, Dickinson And Company Strand displacement amplification
US5994056A (en) 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
US5270184A (en) 1991-11-19 1993-12-14 Becton, Dickinson And Company Nucleic acid target generation
US5925517A (en) 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
DK1921169T3 (da) 1993-11-12 2012-06-04 Phri Properties Inc Hybridiseringssonder til nukleinsyredetektion, universelle stamceller, fremgangsmåder og kits
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
US5648211A (en) 1994-04-18 1997-07-15 Becton, Dickinson And Company Strand displacement amplification using thermophilic enzymes
CA2150986C (en) * 1994-06-17 1999-11-02 Mary Kathryn Meyer Oligonucleotide primers and probes for detection of bacteria
US5641658A (en) 1994-08-03 1997-06-24 Mosaic Technologies, Inc. Method for performing amplification of nucleic acid with two primers bound to a single solid support
US5705628A (en) 1994-09-20 1998-01-06 Whitehead Institute For Biomedical Research DNA purification and isolation using magnetic particles
US5705365A (en) 1995-06-07 1998-01-06 Gen-Probe Incorporated Kits for determining pre-amplification levels of a nucleic acid target sequence from post-amplification levels of product
US5710029A (en) 1995-06-07 1998-01-20 Gen-Probe Incorporated Methods for determining pre-amplification levels of a nucleic acid target sequence from post-amplification levels of product
US5854033A (en) 1995-11-21 1998-12-29 Yale University Rolling circle replication reporter systems
AU713667B2 (en) 1996-04-12 1999-12-09 Phri Properties, Inc. Detection probes, kits and assays
US20100267012A1 (en) 1997-11-04 2010-10-21 Bergeron Michel G Highly conserved genes and their use to generate probes and primers for detection of microorganisms
JP2002503954A (ja) 1997-04-01 2002-02-05 グラクソ、グループ、リミテッド 核酸増幅法
US6465175B2 (en) 1997-09-04 2002-10-15 Bayer Corporation Oligonucleotide probes bearing quenchable fluorescent labels, and methods of use thereof
AR021833A1 (es) 1998-09-30 2002-08-07 Applied Research Systems Metodos de amplificacion y secuenciacion de acido nucleico
EP1020534B2 (en) 1998-11-09 2011-01-05 Eiken Kagaku Kabushiki Kaisha Process for synthesizing nucleic acid
DK1218542T3 (da) 1999-09-13 2004-08-02 Nugen Technologies Inc Fremgangsmåder og sammensætninger til lineær isotermisk amplifikation af polynukleotidsekvenser
EP1246935B1 (en) 1999-09-28 2013-08-14 Geneohm Sciences Canada Inc. Highly conserved genes and their use to generate probes and primers for detection of microorganisms
US7019129B1 (en) 2000-05-09 2006-03-28 Biosearch Technologies, Inc. Dark quenchers for donor-acceptor energy transfer
AUPQ909000A0 (en) * 2000-07-28 2000-08-24 University Of Sydney, The A method of detecting microorganisms
US7001724B1 (en) 2000-11-28 2006-02-21 Applera Corporation Compositions, methods, and kits for isolating nucleic acids using surfactants and proteases
CA2498764C (en) 2002-09-20 2015-11-10 New England Biolabs, Inc. Helicase dependent amplification of nucleic acids
AU2003288018A1 (en) 2002-11-20 2004-06-15 Pharmacia Italia S.P.A. Method for the detection of microorganisms in pharmaceutical products
US20060160153A1 (en) 2002-12-03 2006-07-20 Jean-Pierre Abastado Method to measure a t cell response and its uses to qualify antigen-presenting cells
US20060046246A1 (en) * 2003-04-24 2006-03-02 Qiandong Zeng Genus, group, species and/or strain specific 16S rDNA sequences
CA2562221A1 (en) * 2005-10-21 2007-04-21 Canadian Blood Services A method for detection of staphylococcus epidermidis
CA2684570A1 (en) 2007-04-19 2008-10-30 Molecular Detection Inc. Methods, compositions and kits for detection and analysis of antibiotic-resistant bacteria
US8877060B2 (en) 2010-11-23 2014-11-04 Biovec Transfusion, Llc Methods for removing pathogens from a platelet preparation
BR112012009023A2 (pt) * 2009-10-22 2019-09-24 Biotools Biotechnologial & Medical Laboratories S A método pra detecção. diferenciação e identificação de eubactérias específica de táxons em uma amostra biológica por meio de técnicas de análise de sequenciamento e kit
WO2015013465A2 (en) 2013-07-25 2015-01-29 Dch Molecular Diagnostics, Inc. Methods and compositions for detecting bacterial contamination

Also Published As

Publication number Publication date
CN110592244A (zh) 2019-12-20
JP2016525359A (ja) 2016-08-25
KR102245110B1 (ko) 2021-04-27
CN105473737B (zh) 2019-10-25
US20160355871A1 (en) 2016-12-08
EP3024947B1 (en) 2020-10-28
US20200080134A1 (en) 2020-03-12
WO2015013465A3 (en) 2015-04-23
AU2014293075A1 (en) 2015-12-17
EP3024947A4 (en) 2017-03-15
US10392670B2 (en) 2019-08-27
JP2022023068A (ja) 2022-02-07
MX2016000997A (es) 2016-04-19
SG11201600550WA (en) 2016-02-26
JP2019187431A (ja) 2019-10-31
KR20160034318A (ko) 2016-03-29
US11274351B2 (en) 2022-03-15
TW201525145A (zh) 2015-07-01
TWI697560B (zh) 2020-07-01
WO2015013465A2 (en) 2015-01-29
HK1219298A1 (en) 2017-03-31
EP3024947A2 (en) 2016-06-01
CN105473737A (zh) 2016-04-06
CA2917430A1 (en) 2015-01-29

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