AU2014281026B2 - Delivery, engineering and optimization of tandem guide systems, methods and compositions for sequence manipulation - Google Patents

Delivery, engineering and optimization of tandem guide systems, methods and compositions for sequence manipulation Download PDF

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AU2014281026B2
AU2014281026B2 AU2014281026A AU2014281026A AU2014281026B2 AU 2014281026 B2 AU2014281026 B2 AU 2014281026B2 AU 2014281026 A AU2014281026 A AU 2014281026A AU 2014281026 A AU2014281026 A AU 2014281026A AU 2014281026 B2 AU2014281026 B2 AU 2014281026B2
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sequence
tracr
guide
target
crispr
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AU2014281026A9 (en
AU2014281026A1 (en
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Chie-yu LIN
Fei RAN
Feng Zhang
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Massachusetts Institute of Technology
Broad Institute
Harvard University
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Broad Institute
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1082—Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/35—Nature of the modification
    • C12N2310/351—Conjugate
    • C12N2310/3519—Fusion with another nucleic acid
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    • C12N2800/00—Nucleic acids vectors
    • C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites

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  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
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  • Bioinformatics & Computational Biology (AREA)
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  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
AU2014281026A 2013-06-17 2014-06-10 Delivery, engineering and optimization of tandem guide systems, methods and compositions for sequence manipulation Active AU2014281026B2 (en)

Applications Claiming Priority (15)

Application Number Priority Date Filing Date Title
US201361836123P 2013-06-17 2013-06-17
US61/836,123 2013-06-17
US201361847537P 2013-07-17 2013-07-17
US61/847,537 2013-07-17
US201361862468P 2013-08-05 2013-08-05
US201361862355P 2013-08-05 2013-08-05
US61/862,355 2013-08-05
US61/862,468 2013-08-05
US201361871301P 2013-08-28 2013-08-28
US61/871,301 2013-08-28
US201361915407P 2013-12-12 2013-12-12
US61/915,407 2013-12-12
US201461979942P 2014-04-15 2014-04-15
US61/979,942 2014-04-15
PCT/US2014/041800 WO2014204724A1 (en) 2013-06-17 2014-06-10 Delivery, engineering and optimization of tandem guide systems, methods and compositions for sequence manipulation

Publications (3)

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AU2014281026A1 AU2014281026A1 (en) 2016-01-28
AU2014281026A9 AU2014281026A9 (en) 2016-06-16
AU2014281026B2 true AU2014281026B2 (en) 2020-05-28

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US (2) US11008588B2 (enExample)
EP (2) EP3674411A1 (enExample)
JP (2) JP6625971B2 (enExample)
KR (1) KR20160044457A (enExample)
CN (1) CN106062197A (enExample)
AU (1) AU2014281026B2 (enExample)
CA (1) CA2915834A1 (enExample)
DK (1) DK3011029T3 (enExample)
ES (1) ES2777217T3 (enExample)
WO (1) WO2014204724A1 (enExample)

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