AU2002357423A1 - Magnetism based rapid cell separation - Google Patents

Magnetism based rapid cell separation Download PDF

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Publication number
AU2002357423A1
AU2002357423A1 AU2002357423A AU2002357423A AU2002357423A1 AU 2002357423 A1 AU2002357423 A1 AU 2002357423A1 AU 2002357423 A AU2002357423 A AU 2002357423A AU 2002357423 A AU2002357423 A AU 2002357423A AU 2002357423 A1 AU2002357423 A1 AU 2002357423A1
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AU
Australia
Prior art keywords
virus
magnetic
sample
target cell
cell
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Abandoned
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AU2002357423A
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English (en)
Inventor
Depu Chen
Jing Cheng
Weiyang Fei
Xin Xie
Xu Zhang
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Tsinghua University
CapitalBio Corp
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Tsinghua University
CapitalBio Corp
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Publication of AU2002357423A1 publication Critical patent/AU2002357423A1/en
Assigned to CAPITALBIO CORPORATION., TSINGHUA UNIVERSITY reassignment CAPITALBIO CORPORATION. Request for Assignment Assignors: CAPITAL BIOCHIP COMPANY, LTD., TSINGHUA UNIVERSITY
Abandoned legal-status Critical Current

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54326—Magnetic particles
    • G01N33/54333—Modification of conditions of immunological binding reaction, e.g. use of more than one type of particle, use of chemical agents to improve binding, choice of incubation time or application of magnetic field during binding reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/02—Separating microorganisms from their culture media
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/24—Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54326—Magnetic particles
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/16011—Orthomyxoviridae
    • C12N2760/16051—Methods of production or purification of viral material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2795/00—Bacteriophages
    • C12N2795/00011—Details
    • C12N2795/14011—Details ssDNA Bacteriophages
    • C12N2795/14111—Inoviridae
    • C12N2795/14151—Methods of production or purification of viral material

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Pathology (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Virology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
AU2002357423A 2002-12-09 2002-12-31 Magnetism based rapid cell separation Abandoned AU2002357423A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
CN02153992.8 2002-12-09
CN02153992.8A CN1230531C (zh) 2002-12-09 2002-12-09 从样品中分离细胞粒子的方法
PCT/CN2002/000942 WO2004053115A1 (en) 2002-12-09 2002-12-31 Magnetism based rapid cell separation

Publications (1)

Publication Number Publication Date
AU2002357423A1 true AU2002357423A1 (en) 2004-06-30

Family

ID=32477216

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2002357423A Abandoned AU2002357423A1 (en) 2002-12-09 2002-12-31 Magnetism based rapid cell separation

Country Status (7)

Country Link
US (1) US7776580B2 (https=)
EP (1) EP1578960A4 (https=)
JP (1) JP5399600B2 (https=)
CN (1) CN1230531C (https=)
AU (1) AU2002357423A1 (https=)
CA (1) CA2507592C (https=)
WO (1) WO2004053115A1 (https=)

Families Citing this family (40)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7601491B2 (en) 2003-02-06 2009-10-13 Becton, Dickinson And Company Pretreatment method for extraction of nucleic acid from biological samples and kits therefor
EP1776582B1 (de) * 2004-07-30 2020-06-24 pluriSelect Life Sciences UG & Co. KG Vorrichtung und verfahren zum isolieren von zellen, biopartikeln und/oder molekülen aus flüssigkeiten mit dem ziel einer anwendung für tiere, in der biotechnologie (einschliesslich der biologischen forschung) und der medizinischen diagnostik
DE102005002343A1 (de) 2005-01-18 2006-07-27 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Verfahren zur spezifischen oder unspezifischen Separation von Zellen und/oder Viren aus flüssigen Medien und dessen Verwendung
JP4670583B2 (ja) * 2005-10-20 2011-04-13 チッソ株式会社 水溶性カチオン性磁気微粒子を用いた脂質ベシクルの分離又は検出方法
WO2009035623A1 (en) * 2007-09-11 2009-03-19 Arryx, Inc. Binding method and apparatus for sorting objects
EP2185261A4 (en) * 2007-09-13 2013-08-28 Arryx Inc METHODS AND APPARATUSES FOR SORTING OBJECTS IN MEDICO-LEGAL ANALYSIS OF RNA AND IN MEDICAL DIAGNOSES
GB2455780A (en) * 2007-12-21 2009-06-24 Zainulabedin Mohamedali Saiyed Nucleic acid separation
ES2347300T3 (es) * 2008-02-13 2010-10-27 Inve Technologies Nv Metodo para tratar quistes de artemia.
US20120115167A1 (en) * 2009-04-22 2012-05-10 Clinical Genomics Pty. Ltd. Method and apparatus for isolating a target bioentity from a biological sample
FR2945819B1 (fr) 2009-05-19 2011-06-17 Commissariat Energie Atomique Dispositif et procede d'isolement de cibles biologiques ou chimiques
EP2454020B1 (en) 2009-07-17 2019-05-15 Koninklijke Philips N.V. Apparatus and method for the enrichment of magnetic particles
US8389293B2 (en) 2009-11-17 2013-03-05 Abbott Point Of Care Inc. Reducing leukocyte interference in competitive immunoassays
US8476079B2 (en) 2010-04-30 2013-07-02 Abbott Point Of Care Inc. Reagents for reducing leukocyte interference in immunoassays
US8394325B2 (en) 2010-06-14 2013-03-12 Abbott Point Of Care Inc. Magnetic beads for reducing leukocyte interference in immunoassays
DE102010031401A1 (de) * 2010-07-15 2012-01-19 Aj Innuscreen Gmbh Verfahren zur Anreicherung von Bakterien, Viren sowie Zellen und zur nachfolgenden Nukleinsäureisolierung
DE102010031404A1 (de) * 2010-07-15 2012-01-19 Aj Innuscreen Gmbh Verfahren zur schnellen Bereitstellung von Inhaltsstoffen aus biologischen Proben
CN102343305B (zh) * 2010-08-02 2014-08-27 芮宝生医股份有限公司 一体式反应匣
TW201303296A (zh) * 2011-07-14 2013-01-16 Magqu Co Ltd 用於偵測水產動物病毒之試劑與方法
ES2730711T3 (es) * 2011-11-03 2019-11-12 Qiagen Gaithersburg Inc Materiales y métodos para inmovilizar, aislar y concentrar células usando superficies carboxiladas
US10379026B2 (en) 2012-08-29 2019-08-13 Inguran, Llc Cell processing using magnetic particles
NZ734393A (en) 2012-08-29 2020-02-28 Inguran Llc Magnetic removal or identification of damaged or compromised cells or cellular structures
KR20150063557A (ko) * 2012-10-05 2015-06-09 뉴로바이브 파마슈티컬 에이비 미토콘드리아 독성 테스트
WO2015123664A1 (en) * 2014-02-17 2015-08-20 University Of Maryland, Baltimore Method of extracting lipids from microbes
KR101728096B1 (ko) 2015-03-12 2017-04-20 재단법인 아산사회복지재단 Ffpe 조직에서 핵산의 분리 방법
KR101967878B1 (ko) * 2015-03-12 2019-04-11 재단법인 아산사회복지재단 순환 유리 핵산의 분리 방법
WO2016183032A1 (en) 2015-05-08 2016-11-17 Biomagnetic Solutions Llc Apparatus and method for immunomagnetic cell separation
US12339276B2 (en) 2016-08-31 2025-06-24 Board Of Trustees Of Michigan State University Functionalized magnetic particle compositions and related methods
WO2018062573A1 (ja) * 2016-09-29 2018-04-05 栄研化学株式会社 細胞の回収方法
DE102017204267B4 (de) 2017-03-14 2021-05-27 Aj Innuscreen Gmbh Verfahren zur anreicherung von zellen aus einer probe und der nachfolgenden nukleinsäureisolierung aus diesen zellen
US11433402B2 (en) 2017-07-19 2022-09-06 Amgen Inc. Magnetic assisted separation apparatuses and related methods
CN112970076A (zh) * 2018-07-19 2021-06-15 贝克曼库尔特有限公司 磁性颗粒
DE102019118332B4 (de) * 2019-07-07 2022-04-07 Ist Innuscreen Gmbh Verfahren und testkit zur bisulfitmodifizierung von dna
KR102298909B1 (ko) * 2019-10-25 2021-09-06 주식회사 싸이토딕스 역류 원심분리를 이용한 ctc 분리방법
KR102298910B1 (ko) * 2019-10-25 2021-09-06 주식회사 싸이토딕스 역류 원심분리를 이용한 ctc 분리방법
CN110734898B (zh) * 2019-11-28 2021-07-20 中国疾病预防控制中心病毒病预防控制所 病毒生物磁珠富集浓缩试剂盒及应用
JP2024505224A (ja) * 2021-01-29 2024-02-05 アボット・ダイアグノスティックス・スカボロー・インコーポレイテッド 磁性粒子空気移送
CN113293159A (zh) * 2021-06-21 2021-08-24 清华大学深圳国际研究生院 一种核酸提取试剂盒及核酸提取方法
CN114214225B (zh) * 2021-11-28 2023-12-15 中国人民解放军军事科学院军事医学研究院 一种富集新月柄杆菌分泌的holdfast的方法
US20250361483A1 (en) * 2022-10-24 2025-11-27 National Resilience, Inc. Systems, Methods, and Compositions for Selecting or Isolating Cells
WO2025090532A1 (en) * 2023-10-23 2025-05-01 Ctrl Therapeutics Usa, Inc. Platforms, cartridges, and methods for isolation and enrichment of target cells

Family Cites Families (40)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE278598C (https=)
US4001197A (en) * 1975-06-12 1977-01-04 Sala Magnetics, Inc. Magnetic separation method
AU530410B2 (en) * 1978-02-21 1983-07-14 Sintef Preparing aqueous emulsions
US4230685A (en) * 1979-02-28 1980-10-28 Northwestern University Method of magnetic separation of cells and the like, and microspheres for use therein
US4421660A (en) * 1980-12-15 1983-12-20 The Dow Chemical Company Colloidal size hydrophobic polymers particulate having discrete particles of an inorganic material dispersed therein
JPS5876435A (ja) * 1981-10-30 1983-05-09 Japan Synthetic Rubber Co Ltd 重合体粒子
NO155316C (no) 1982-04-23 1987-03-11 Sintef Fremgangsmaate for fremstilling av magnetiske polymerpartikler.
US4554088A (en) * 1983-05-12 1985-11-19 Advanced Magnetics Inc. Magnetic particles for use in separations
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US5076950A (en) * 1985-12-20 1991-12-31 Syntex (U.S.A.) Inc. Magnetic composition for particle separation
US5395688A (en) * 1987-10-26 1995-03-07 Baxter Diagnostics Inc. Magnetically responsive fluorescent polymer particles
US5091206A (en) * 1987-10-26 1992-02-25 Baxter Diagnostics Inc. Process for producing magnetically responsive polymer particles and application thereof
CA1340807C (en) * 1988-02-24 1999-11-02 Lawrence T. Malek Nucleic acid amplification process
JP2650159B2 (ja) * 1988-02-24 1997-09-03 アクゾ・ノベル・エヌ・ベー 核酸増幅方法
US4965007A (en) * 1988-05-10 1990-10-23 Eastman Kodak Company Encapsulated superparamagnetic particles
DD278598A1 (de) * 1988-12-23 1990-05-09 Univ Leipzig Verfahren zur abtrennung von bakterien aus hochviskosen, saeurehaltigen suspensionen
US5232789A (en) * 1989-03-09 1993-08-03 Mtu Motoren- Und Turbinen-Union Muenchen Gmbh Structural component with a protective coating having a nickel or cobalt basis and method for making such a coating
US5279936A (en) * 1989-12-22 1994-01-18 Syntex (U.S.A.) Inc. Method of separation employing magnetic particles and second medium
GB9003253D0 (en) * 1990-02-13 1990-04-11 Amersham Int Plc Precipitating polymers
US5318797A (en) * 1990-06-20 1994-06-07 Clarkson University Coated particles, hollow particles, and process for manufacturing the same
US5491068A (en) * 1991-02-14 1996-02-13 Vicam, L.P. Assay method for detecting the presence of bacteria
GB9107124D0 (en) 1991-04-05 1991-05-22 Dynal As Chemical process
US5686271A (en) 1994-06-09 1997-11-11 Gamera Bioscience Corporation Apparatus for performing magnetic cycle reaction
US5876924A (en) 1994-06-22 1999-03-02 Mount Sinai School Of Medicine Nucleic acid amplification method hybridization signal amplification method (HSAM)
US5705059A (en) * 1995-02-27 1998-01-06 Miltenyi; Stefan Magnetic separation apparatus
EP0768530A1 (en) 1995-10-16 1997-04-16 Nippon Paint Co., Ltd. Process for assaying biological substance
US5834121A (en) * 1996-01-16 1998-11-10 Solid Phase Sciences Corp. Composite magnetic beads
US5968820A (en) 1997-02-26 1999-10-19 The Cleveland Clinic Foundation Method for magnetically separating cells into fractionated flow streams
GB9709728D0 (en) * 1997-05-13 1997-07-02 Dynal As Single step method
DE19851156C1 (de) 1998-11-06 2000-04-27 Merck Patent Gmbh Verfahren zur Isolierung von Plasmid-DNA
WO2000061803A1 (en) 1999-04-13 2000-10-19 Nanogen, Inc. Magnetic bead-based array for genetic detection
EP1108743B1 (en) 1999-12-08 2006-11-15 JSR Corporation Separation of viruses and detection of viruses
AU2001234595A1 (en) 2000-01-26 2001-08-07 University Of Cincinnati Detection of nucleic acid target sequences by electron paramagnetic resonance spectroscopy
JP4684434B2 (ja) * 2000-02-16 2011-05-18 Ｊｓｒ株式会社 ウイルス濃縮用粒子、ウィルス濃縮用試薬、ウイルス濃縮方法およびウイルス検出方法
KR20030032922A (ko) * 2000-02-24 2003-04-26 싸이트 테라피스 인코포레이티드 세포의 동시 자극 및 농축
EP1272668B1 (en) * 2000-04-03 2007-02-14 Corixa Corporation Methods, compositions and kits for the detection and monitoring of breast cancer
CN1152055C (zh) 2001-03-20 2004-06-02 清华大学 磁性微球的表面包覆和基团功能化修饰方法及所得微球及其应用
KR100445560B1 (ko) * 2001-10-31 2004-08-21 (주)바이오넥스 핵산 또는 생물학적 물질을 분리하기 위한 키트의 제조방법과, 그 방법에 의해 제조된 키트와, 그 키트를사용하는 장치
CN1304589C (zh) * 2001-12-07 2007-03-14 清华大学 利用固相载体从生物粒子中分离核酸的方法

Also Published As

Publication number Publication date
CN1230531C (zh) 2005-12-07
JP5399600B2 (ja) 2014-01-29
EP1578960A1 (en) 2005-09-28
CA2507592A1 (en) 2004-06-24
CN1506460A (zh) 2004-06-23
CA2507592C (en) 2013-12-03
US7776580B2 (en) 2010-08-17
JP2006508668A (ja) 2006-03-16
US20060141450A1 (en) 2006-06-29
EP1578960A4 (en) 2006-10-11
WO2004053115A1 (en) 2004-06-24

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Owner name: TSINGHUA UNIVERSITY; CAPITALBIO CORPORATION.

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