CN1506460A - 从样品中分离细胞粒子的方法及其试剂盒 - Google Patents
从样品中分离细胞粒子的方法及其试剂盒 Download PDFInfo
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- CN1506460A CN1506460A CNA021539928A CN02153992A CN1506460A CN 1506460 A CN1506460 A CN 1506460A CN A021539928 A CNA021539928 A CN A021539928A CN 02153992 A CN02153992 A CN 02153992A CN 1506460 A CN1506460 A CN 1506460A
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- beads
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
- G01N33/54326—Magnetic particles
- G01N33/54333—Modification of conditions of immunological binding reaction, e.g. use of more than one type of particle, use of chemical agents to improve binding, choice of incubation time or application of magnetic field during binding reaction
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/02—Separating microorganisms from their culture media
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/24—Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
- G01N33/54326—Magnetic particles
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
- C12N2760/00011—Details
- C12N2760/16011—Orthomyxoviridae
- C12N2760/16051—Methods of production or purification of viral material
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2795/00—Bacteriophages
- C12N2795/00011—Details
- C12N2795/14011—Details ssDNA Bacteriophages
- C12N2795/14111—Inoviridae
- C12N2795/14151—Methods of production or purification of viral material
Abstract
Description
剂名 | 抗凝力 |
10%草酸钾 | 0.2ml抗凝10ml的血 |
草酸钠 | 1~2mg抗凝1ml的血 |
3.8%枸橼酸钠 | 6mg抗凝1ml的血 |
双草酸盐(草酸钾、草酸胺) | 0.5ml抗凝5ml的血 |
草酸钾氟化钠 | 0.1ml抗凝1ml的血 |
肝素 | 0.1ml抗凝5~10ml的血 |
Claims (55)
Priority Applications (7)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN02153992.8A CN1230531C (zh) | 2002-12-09 | 2002-12-09 | 从样品中分离细胞粒子的方法 |
PCT/CN2002/000942 WO2004053115A1 (en) | 2002-12-09 | 2002-12-31 | Magnetism based rapid cell separation |
EP02808210A EP1578960A4 (en) | 2002-12-09 | 2002-12-31 | QUICK CELL SEPARATION BASED ON MAGNETISM |
AU2002357423A AU2002357423A1 (en) | 2002-12-09 | 2002-12-31 | Magnetism based rapid cell separation |
US10/538,498 US7776580B2 (en) | 2002-12-09 | 2002-12-31 | Magnetism based rapid cell separation |
CA2507592A CA2507592C (en) | 2002-12-09 | 2002-12-31 | Magnetism based rapid cell separation |
JP2004557741A JP5399600B2 (ja) | 2002-12-09 | 2002-12-31 | 磁性ベースの迅速な細胞分離 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN02153992.8A CN1230531C (zh) | 2002-12-09 | 2002-12-09 | 从样品中分离细胞粒子的方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN1506460A true CN1506460A (zh) | 2004-06-23 |
CN1230531C CN1230531C (zh) | 2005-12-07 |
Family
ID=32477216
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN02153992.8A Expired - Fee Related CN1230531C (zh) | 2002-12-09 | 2002-12-09 | 从样品中分离细胞粒子的方法 |
Country Status (7)
Country | Link |
---|---|
US (1) | US7776580B2 (zh) |
EP (1) | EP1578960A4 (zh) |
JP (1) | JP5399600B2 (zh) |
CN (1) | CN1230531C (zh) |
AU (1) | AU2002357423A1 (zh) |
CA (1) | CA2507592C (zh) |
WO (1) | WO2004053115A1 (zh) |
Cited By (11)
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CN102343305A (zh) * | 2010-08-02 | 2012-02-08 | 芮宝生医股份有限公司 | 一体式反应匣 |
CN102458665A (zh) * | 2009-04-22 | 2012-05-16 | 临床基因组学股份有限公司 | 用于从生物学样品中分离靶生物实体的方法和仪器 |
CN102470373A (zh) * | 2009-07-17 | 2012-05-23 | 皇家飞利浦电子股份有限公司 | 用于富集磁性粒子的设备 |
CN101057141B (zh) * | 2004-07-30 | 2012-11-07 | 普拉里斯莱克特有限公司 | 一种从流体中分离细胞、生物颗粒和/或分子以应用于动物、生物技术(包括生物学研究)和医疗诊断的方法和仪器 |
CN102879564A (zh) * | 2011-07-14 | 2013-01-16 | 磁量生技股份有限公司 | 用于检测水产动物病毒的检测试剂 |
CN104704361A (zh) * | 2012-10-05 | 2015-06-10 | 纽维制药有限公司 | 线粒体毒性检测 |
CN107530486A (zh) * | 2015-05-08 | 2018-01-02 | 生物磁溶液有限公司 | 免疫磁性细胞分离的装置和方法 |
CN110734898A (zh) * | 2019-11-28 | 2020-01-31 | 中国疾病预防控制中心病毒病预防控制所 | 病毒生物磁珠富集浓缩试剂盒及应用 |
CN110869130A (zh) * | 2017-07-19 | 2020-03-06 | 美国安进公司 | 磁性辅助分离装置及相关方法 |
CN110945124A (zh) * | 2017-03-14 | 2020-03-31 | Aj耶拿检疫有限公司 | 用于从样品中富集细胞并且后续从这些细胞中分离核酸的方法 |
CN112970076A (zh) * | 2018-07-19 | 2021-06-15 | 贝克曼库尔特有限公司 | 磁性颗粒 |
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DE102005002343A1 (de) | 2005-01-18 | 2006-07-27 | Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. | Verfahren zur spezifischen oder unspezifischen Separation von Zellen und/oder Viren aus flüssigen Medien und dessen Verwendung |
JP4670583B2 (ja) * | 2005-10-20 | 2011-04-13 | チッソ株式会社 | 水溶性カチオン性磁気微粒子を用いた脂質ベシクルの分離又は検出方法 |
US8067170B2 (en) * | 2007-09-11 | 2011-11-29 | Arryx, Inc. | Binding method and apparatus for sorting objects |
EP2185261A4 (en) * | 2007-09-13 | 2013-08-28 | Arryx Inc | METHODS AND APPARATUSES FOR SORTING OBJECTS IN MEDICO-LEGAL ANALYSIS OF RNA AND IN MEDICAL DIAGNOSES |
GB2455780A (en) * | 2007-12-21 | 2009-06-24 | Zainulabedin Mohamedali Saiyed | Nucleic acid separation |
ES2347300T3 (es) * | 2008-02-13 | 2010-10-27 | Inve Technologies Nv | Metodo para tratar quistes de artemia. |
FR2945819B1 (fr) | 2009-05-19 | 2011-06-17 | Commissariat Energie Atomique | Dispositif et procede d'isolement de cibles biologiques ou chimiques |
US8389293B2 (en) | 2009-11-17 | 2013-03-05 | Abbott Point Of Care Inc. | Reducing leukocyte interference in competitive immunoassays |
US8476079B2 (en) | 2010-04-30 | 2013-07-02 | Abbott Point Of Care Inc. | Reagents for reducing leukocyte interference in immunoassays |
US8394325B2 (en) | 2010-06-14 | 2013-03-12 | Abbott Point Of Care Inc. | Magnetic beads for reducing leukocyte interference in immunoassays |
DE102010031401A1 (de) * | 2010-07-15 | 2012-01-19 | Aj Innuscreen Gmbh | Verfahren zur Anreicherung von Bakterien, Viren sowie Zellen und zur nachfolgenden Nukleinsäureisolierung |
DE102010031404A1 (de) * | 2010-07-15 | 2012-01-19 | Aj Innuscreen Gmbh | Verfahren zur schnellen Bereitstellung von Inhaltsstoffen aus biologischen Proben |
JP6103778B2 (ja) * | 2011-11-03 | 2017-03-29 | キアジェン ゲイサーズバーグ インコーポレイテッド | カルボキシル化表面を使用して細胞を固定、単離、および濃縮するための材料および方法 |
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-
2002
- 2002-12-09 CN CN02153992.8A patent/CN1230531C/zh not_active Expired - Fee Related
- 2002-12-31 JP JP2004557741A patent/JP5399600B2/ja not_active Expired - Fee Related
- 2002-12-31 CA CA2507592A patent/CA2507592C/en not_active Expired - Fee Related
- 2002-12-31 US US10/538,498 patent/US7776580B2/en not_active Expired - Fee Related
- 2002-12-31 EP EP02808210A patent/EP1578960A4/en not_active Withdrawn
- 2002-12-31 WO PCT/CN2002/000942 patent/WO2004053115A1/en active Application Filing
- 2002-12-31 AU AU2002357423A patent/AU2002357423A1/en not_active Abandoned
Cited By (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101057141B (zh) * | 2004-07-30 | 2012-11-07 | 普拉里斯莱克特有限公司 | 一种从流体中分离细胞、生物颗粒和/或分子以应用于动物、生物技术(包括生物学研究)和医疗诊断的方法和仪器 |
CN102458665A (zh) * | 2009-04-22 | 2012-05-16 | 临床基因组学股份有限公司 | 用于从生物学样品中分离靶生物实体的方法和仪器 |
US9272290B2 (en) | 2009-07-17 | 2016-03-01 | Koninklijke Philips N.V. | Apparatus for the enrichment of magnetic particles |
CN102470373A (zh) * | 2009-07-17 | 2012-05-23 | 皇家飞利浦电子股份有限公司 | 用于富集磁性粒子的设备 |
CN102470373B (zh) * | 2009-07-17 | 2014-11-26 | 皇家飞利浦电子股份有限公司 | 用于富集磁性粒子的设备 |
CN102343305A (zh) * | 2010-08-02 | 2012-02-08 | 芮宝生医股份有限公司 | 一体式反应匣 |
CN102343305B (zh) * | 2010-08-02 | 2014-08-27 | 芮宝生医股份有限公司 | 一体式反应匣 |
CN102879564A (zh) * | 2011-07-14 | 2013-01-16 | 磁量生技股份有限公司 | 用于检测水产动物病毒的检测试剂 |
CN104704361A (zh) * | 2012-10-05 | 2015-06-10 | 纽维制药有限公司 | 线粒体毒性检测 |
CN107530486A (zh) * | 2015-05-08 | 2018-01-02 | 生物磁溶液有限公司 | 免疫磁性细胞分离的装置和方法 |
CN110945124A (zh) * | 2017-03-14 | 2020-03-31 | Aj耶拿检疫有限公司 | 用于从样品中富集细胞并且后续从这些细胞中分离核酸的方法 |
CN110869130A (zh) * | 2017-07-19 | 2020-03-06 | 美国安进公司 | 磁性辅助分离装置及相关方法 |
US11433402B2 (en) | 2017-07-19 | 2022-09-06 | Amgen Inc. | Magnetic assisted separation apparatuses and related methods |
CN112970076A (zh) * | 2018-07-19 | 2021-06-15 | 贝克曼库尔特有限公司 | 磁性颗粒 |
CN110734898A (zh) * | 2019-11-28 | 2020-01-31 | 中国疾病预防控制中心病毒病预防控制所 | 病毒生物磁珠富集浓缩试剂盒及应用 |
CN110734898B (zh) * | 2019-11-28 | 2021-07-20 | 中国疾病预防控制中心病毒病预防控制所 | 病毒生物磁珠富集浓缩试剂盒及应用 |
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US20060141450A1 (en) | 2006-06-29 |
EP1578960A4 (en) | 2006-10-11 |
WO2004053115A1 (en) | 2004-06-24 |
US7776580B2 (en) | 2010-08-17 |
CA2507592C (en) | 2013-12-03 |
JP2006508668A (ja) | 2006-03-16 |
CN1230531C (zh) | 2005-12-07 |
AU2002357423A1 (en) | 2004-06-30 |
EP1578960A1 (en) | 2005-09-28 |
CA2507592A1 (en) | 2004-06-24 |
JP5399600B2 (ja) | 2014-01-29 |
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