CN1230531C - 从样品中分离细胞粒子的方法 - Google Patents

从样品中分离细胞粒子的方法 Download PDF

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Publication number
CN1230531C
CN1230531C CN02153992.8A CN02153992A CN1230531C CN 1230531 C CN1230531 C CN 1230531C CN 02153992 A CN02153992 A CN 02153992A CN 1230531 C CN1230531 C CN 1230531C
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China
Prior art keywords
magnetic
sample
viruses
target cells
organelles
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Expired - Fee Related
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CN02153992.8A
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English (en)
Chinese (zh)
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CN1506460A (zh
Inventor
张旭
谢欣
陈德朴
费维扬
程京
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Tsinghua University
CapitalBio Corp
Original Assignee
BOAO BIOCHIP Co Ltd BEIJING
Tsinghua University
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Priority to CN02153992.8A priority Critical patent/CN1230531C/zh
Application filed by BOAO BIOCHIP Co Ltd BEIJING, Tsinghua University filed Critical BOAO BIOCHIP Co Ltd BEIJING
Priority to JP2004557741A priority patent/JP5399600B2/ja
Priority to US10/538,498 priority patent/US7776580B2/en
Priority to EP02808210A priority patent/EP1578960A4/en
Priority to PCT/CN2002/000942 priority patent/WO2004053115A1/en
Priority to CA2507592A priority patent/CA2507592C/en
Priority to AU2002357423A priority patent/AU2002357423A1/en
Publication of CN1506460A publication Critical patent/CN1506460A/zh
Application granted granted Critical
Publication of CN1230531C publication Critical patent/CN1230531C/zh
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54326—Magnetic particles
    • G01N33/54333—Modification of conditions of immunological binding reaction, e.g. use of more than one type of particle, use of chemical agents to improve binding, choice of incubation time or application of magnetic field during binding reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/02—Separating microorganisms from their culture media
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/24—Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54326—Magnetic particles
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/16011—Orthomyxoviridae
    • C12N2760/16051—Methods of production or purification of viral material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2795/00—Bacteriophages
    • C12N2795/00011—Details
    • C12N2795/14011—Details ssDNA Bacteriophages
    • C12N2795/14111—Inoviridae
    • C12N2795/14151—Methods of production or purification of viral material

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Pathology (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Virology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
CN02153992.8A 2002-12-09 2002-12-09 从样品中分离细胞粒子的方法 Expired - Fee Related CN1230531C (zh)

Priority Applications (7)

Application Number Priority Date Filing Date Title
CN02153992.8A CN1230531C (zh) 2002-12-09 2002-12-09 从样品中分离细胞粒子的方法
US10/538,498 US7776580B2 (en) 2002-12-09 2002-12-31 Magnetism based rapid cell separation
EP02808210A EP1578960A4 (en) 2002-12-09 2002-12-31 RAPID CELL SEPARATION ON MAGNET BASE
PCT/CN2002/000942 WO2004053115A1 (en) 2002-12-09 2002-12-31 Magnetism based rapid cell separation
JP2004557741A JP5399600B2 (ja) 2002-12-09 2002-12-31 磁性ベースの迅速な細胞分離
CA2507592A CA2507592C (en) 2002-12-09 2002-12-31 Magnetism based rapid cell separation
AU2002357423A AU2002357423A1 (en) 2002-12-09 2002-12-31 Magnetism based rapid cell separation

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN02153992.8A CN1230531C (zh) 2002-12-09 2002-12-09 从样品中分离细胞粒子的方法

Publications (2)

Publication Number Publication Date
CN1506460A CN1506460A (zh) 2004-06-23
CN1230531C true CN1230531C (zh) 2005-12-07

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CN02153992.8A Expired - Fee Related CN1230531C (zh) 2002-12-09 2002-12-09 从样品中分离细胞粒子的方法

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US (1) US7776580B2 (https=)
EP (1) EP1578960A4 (https=)
JP (1) JP5399600B2 (https=)
CN (1) CN1230531C (https=)
AU (1) AU2002357423A1 (https=)
CA (1) CA2507592C (https=)
WO (1) WO2004053115A1 (https=)

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Publication number Publication date
JP5399600B2 (ja) 2014-01-29
EP1578960A1 (en) 2005-09-28
CA2507592A1 (en) 2004-06-24
CN1506460A (zh) 2004-06-23
CA2507592C (en) 2013-12-03
US7776580B2 (en) 2010-08-17
JP2006508668A (ja) 2006-03-16
US20060141450A1 (en) 2006-06-29
EP1578960A4 (en) 2006-10-11
WO2004053115A1 (en) 2004-06-24
AU2002357423A1 (en) 2004-06-30

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