ATE445709T1 - Glycoproteinsynthese - Google Patents

Glycoproteinsynthese

Info

Publication number
ATE445709T1
ATE445709T1 AT03777634T AT03777634T ATE445709T1 AT E445709 T1 ATE445709 T1 AT E445709T1 AT 03777634 T AT03777634 T AT 03777634T AT 03777634 T AT03777634 T AT 03777634T AT E445709 T1 ATE445709 T1 AT E445709T1
Authority
AT
Austria
Prior art keywords
amino acid
unnatural amino
protein
methods
method involves
Prior art date
Application number
AT03777634T
Other languages
English (en)
Inventor
Peter Schultz
Lei Wang
Zhiwen Zhang
Original Assignee
Scripps Research Inst
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Scripps Research Inst filed Critical Scripps Research Inst
Application granted granted Critical
Publication of ATE445709T1 publication Critical patent/ATE445709T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
AT03777634T 2002-10-16 2003-10-15 Glycoproteinsynthese ATE445709T1 (de)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US41926502P 2002-10-16 2002-10-16
US42099002P 2002-10-23 2002-10-23
US44145003P 2003-01-16 2003-01-16
PCT/US2003/032870 WO2004035605A2 (en) 2002-10-16 2003-10-15 Glycoprotein synthesis

Publications (1)

Publication Number Publication Date
ATE445709T1 true ATE445709T1 (de) 2009-10-15

Family

ID=32110831

Family Applications (1)

Application Number Title Priority Date Filing Date
AT03777634T ATE445709T1 (de) 2002-10-16 2003-10-15 Glycoproteinsynthese

Country Status (13)

Country Link
US (11) US6927042B2 (de)
EP (1) EP1558747B1 (de)
JP (1) JP4752001B2 (de)
KR (1) KR20050073559A (de)
AT (1) ATE445709T1 (de)
AU (1) AU2003286436B2 (de)
CA (1) CA2500653A1 (de)
DE (1) DE60329700D1 (de)
ES (1) ES2333422T3 (de)
IL (6) IL196905A (de)
MX (1) MXPA05003978A (de)
SG (1) SG160203A1 (de)
WO (1) WO2004035605A2 (de)

Families Citing this family (114)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2005502322A (ja) * 2001-04-19 2005-01-27 ザ スクリップス リサーチ インスティテュート 非天然アミノ酸のインビボ組込み
US8414774B2 (en) * 2001-04-25 2013-04-09 Agilent Technologies, Inc. Systems and methods for high-throughput screening of fluidic samples
US20050123970A1 (en) * 2001-04-25 2005-06-09 Can Ozbal High throughput autosampler
EP1558747B1 (de) * 2002-10-16 2009-10-14 The Scripps Research Institute Glycoproteinsynthese
JP4444113B2 (ja) 2002-10-16 2010-03-31 ザ スクリップス リサーチ インスティテュート ケトアミノ酸の部位特異的蛋白質組込み方法
CN103215227B (zh) 2003-04-17 2015-09-16 斯克利普斯研究院 扩展真核生物遗传密码
JP5275566B2 (ja) 2003-06-18 2013-08-28 ザ スクリプス リサーチ インスティチュート 反応性非天然アミノ酸遺伝コード付加
ES2301099T3 (es) * 2003-07-07 2008-06-16 The Scripps Research Institute Composiciones de parejas ortogonales lisil-arnt y aminoacil-arnt sintetasa y usos de las mismas.
EP1704242A4 (de) * 2003-07-07 2008-06-18 Scripps Research Inst ZUSAMMENSETZUNGEN MIT PAAREN VON ORTHOGONALER LEUCYL-tRNA UND AMINOACYL-tRNA-SYNTHETASE UND VERWENDUNGEN DAVON
WO2005007624A2 (en) * 2003-07-07 2005-01-27 The Scripps Research Institute Compositions of orthogonal glutamyl-trna and aminoacyl trna synthetase pairs and uses thereof
WO2005038002A2 (en) 2003-10-14 2005-04-28 The Scripps Research Institute Site-specific incorporation of redox active amino acids into proteins
NZ548255A (en) * 2004-02-02 2010-10-29 Ambrx Inc Modified human interferon polypeptides and their uses
US20050260711A1 (en) * 2004-03-30 2005-11-24 Deepshikha Datta Modulating pH-sensitive binding using non-natural amino acids
US20050287639A1 (en) * 2004-05-17 2005-12-29 California Institute Of Technology Methods of incorporating amino acid analogs into proteins
MXPA06013586A (es) * 2004-05-25 2007-11-09 Scripss Res Inst Incorporacion especifica del sitio de aminoacidos no naturales que contienen atomos pesados en proteinas para la determinacion de la estructura cristalina.
KR20070034512A (ko) 2004-06-18 2007-03-28 암브룩스, 인코포레이티드 신규 항원-결합 폴리펩티드 및 이의 용도
EP1771573A4 (de) * 2004-07-21 2009-02-18 Ambrx Inc Nichtnatürliche kodierte aminosäuren verwendende biosynthetische polypeptide
AU2009212311B8 (en) 2004-07-21 2013-05-30 Ambrx, Inc. Modified leptin polypeptides and their uses
US20060134746A1 (en) * 2004-09-21 2006-06-22 The Scripps Research Institute Adding photoregulated amino acids to the genetic code
CN101151366B (zh) 2004-09-21 2012-12-19 斯克利普斯研究院 将炔基氨基酸体内掺入真细菌的蛋白
EP1794312A4 (de) * 2004-09-22 2009-08-12 Scripps Research Inst Ortsspezifische etikettierung von proteinen für nmr-studien
EP1809758A4 (de) 2004-10-20 2009-04-01 Scripps Research Inst Stellenspezifischer in-vivo-einbau von n-acetylgalactosamin-aminosäuren in eubakterien
US8216804B2 (en) 2004-10-27 2012-07-10 The Scripps Research Institute Orthogonal translation components for the in vivo incorporation of unnatural amino acids
EP2586456B1 (de) 2004-10-29 2016-01-20 ratiopharm GmbH Umformung und GlycoPEGylierung des Fibroblastenwachstumsfaktors (FGF)
WO2006073846A2 (en) 2004-12-22 2006-07-13 Ambrx, Inc. Methods for expression and purification of recombinant human growth hormone
ATE541934T1 (de) 2004-12-22 2012-02-15 Ambrx Inc Zusammensetzungen von aminoacyl-trna-synthetase und verwendungen davon
NZ555386A (en) 2004-12-22 2011-01-28 Ambrx Inc Formulations of human growth hormone comprising a non-naturally encoded amino acid
JP2008525473A (ja) 2004-12-22 2008-07-17 アンブレツクス・インコーポレイテツド 修飾されたヒト成長ホルモン
MX2007015058A (es) 2005-06-03 2008-01-28 Ambrx Inc Moleculas de interferon humano mejoradas y sus usos.
KR101285904B1 (ko) 2005-08-18 2013-07-15 암브룩스, 인코포레이티드 ｔＲＮＡ 조성물 및 이의 용도
AU2006304110B2 (en) * 2005-10-12 2012-02-02 The Scripps Research Institute Selective posttranslational modification of phage-displayed polypeptides
CN101454461A (zh) * 2005-11-16 2009-06-10 Ambrx公司 包括非天然氨基酸的方法和组合物
US7776535B2 (en) * 2006-02-06 2010-08-17 Franklin And Marshall College Site-specific incorporation of fluorinated amino acids into proteins
ES2504521T3 (es) 2006-03-03 2014-10-08 California Institute Of Technology Incorporación específica del sitio de aminoácidos en moléculas
CN101400796B (zh) 2006-03-09 2013-03-27 斯克利普斯研究院 在真细菌宿主细胞内表达正交翻译组分的系统
RU2464315C2 (ru) * 2006-03-16 2012-10-20 Зе Скрипс Ресеч Инститьют Генетически запрограммированная экспрессия белков, содержащих не встречающуюся в природе аминокислоту фенилселеноцистеин
EP2395099A3 (de) 2006-05-02 2012-05-16 Allozyne, Inc. Aminosäuresubstituierte Moleküle
US7807411B2 (en) * 2006-05-23 2010-10-05 The Scripps Research Institute Genetically encoded fluorescent coumarin amino acids
AU2007292892B9 (en) 2006-09-08 2013-02-28 Ambrx, Inc. Hybrid suppressor tRNA for vertebrate cells
AU2007292893B2 (en) 2006-09-08 2012-03-01 Ambrx, Inc. Suppressor tRNA transcription in vertebrate cells
CN106008699A (zh) 2006-09-08 2016-10-12 Ambrx公司 经修饰的人类血浆多肽或Fc骨架和其用途
EP2052083B1 (de) * 2006-09-21 2014-01-08 The Scripps Research Institute Genetisch programmierte expression von selektiv sulfatierten proteinen in eubakterien
EP2069481A4 (de) 2006-10-18 2010-01-06 Scripps Research Inst Genetischer einbau unnatürlicher aminosäuren in proteine in säugerzellen
US7403686B1 (en) * 2006-11-30 2008-07-22 The United States Of America As Represented By The Secretary Of The Navy Fiber optic cable splicing technique
EP2112928A4 (de) * 2007-02-22 2014-01-08 Univ Utah Res Found Synthese von neuartigen xylosiden und ihre mögliche verwendung
EP2068909B1 (de) 2007-03-30 2012-04-25 Ambrx, Inc. Modifizierte fgf-21 polypeptide und ihre verwendung
CN101675071B (zh) 2007-05-02 2014-06-18 Ambrx公司 经修饰干扰素β多肽和其用途
CA2707840A1 (en) 2007-08-20 2009-02-26 Allozyne, Inc. Amino acid substituted molecules
JP5643100B2 (ja) 2007-10-25 2014-12-17 ザ スクリプス リサーチ インスティテュート レダクターゼへの３−アミノチロシンの遺伝的組込み
FR2922769A1 (fr) * 2007-10-31 2009-05-01 Halina Malina Modification des sequences intrinsequement desordonnees pour la preparation de vaccins
EP2217702A4 (de) 2007-11-02 2012-05-16 Scripps Research Inst Gerichtete evolution unter verwendung von proteinen mit nicht natürlichen aminosäuren
CA2703991A1 (en) * 2007-11-02 2009-05-07 Biotrove, Inc. Devices and methods for coupling mass spectrometry devices with chromatography systems
WO2009059056A2 (en) * 2007-11-02 2009-05-07 The Scripps Research Institute A genetically encoded boronate amino acid
ES2632504T3 (es) 2007-11-20 2017-09-13 Ambrx, Inc. Polipéptidos de insulina modificados y sus usos
EP2889370A1 (de) 2007-12-11 2015-07-01 The Scripps Research Institute Unnatürliche In-vivo-Aminosäure-Expression bei der Methylotrophen-Hefe Pichia pastoris
EP2240595B2 (de) 2008-01-03 2019-07-24 Cornell Research Foundation, Inc. Expression eines glycosylierten proteins in prokaryonten
JP2011514322A (ja) * 2008-02-08 2011-05-06 ザ スクリプス リサーチ インスティチュート 遺伝的にコードされる非天然アミノ酸による免疫寛容解除
WO2009151491A2 (en) * 2008-02-27 2009-12-17 The Scripps Research Institute In vivo incorporation of an unnatural amino acid comprising a 1,2-aminothiol group
WO2009133208A1 (en) 2008-05-02 2009-11-05 Novartis Ag Improved fibronectin-based binding molecules and uses thereof
CN106928339A (zh) 2008-07-23 2017-07-07 Ambrx 公司 经修饰的牛g‑csf多肽和其用途
PT2337846T (pt) 2008-09-26 2018-04-05 Ambrx Inc Vacinas e microrganismos dependentes da replicação de aminoácidos não naturais
CA2737026C (en) 2008-09-26 2019-12-24 Ambrx, Inc. Modified animal erythropoietin polypeptides and their uses
US20090197339A1 (en) * 2008-12-10 2009-08-06 The Scripps Research Institute In vivo unnatural amino acid expression in the methylotrophic yeast pichia pastoris
WO2010068278A2 (en) 2008-12-10 2010-06-17 The Scripps Research Institute Production of carrier-peptide conjugates using chemically reactive unnatural amino acids
WO2010096394A2 (en) 2009-02-17 2010-08-26 Redwood Biosciences, Inc. Aldehyde-tagged protein-based drug carriers and methods of use
JP6091892B2 (ja) * 2009-06-16 2017-03-08 グライセラ・リミテッド グリコシル化に関連する物質および方法
WO2011051327A2 (en) 2009-10-30 2011-05-05 Novartis Ag Small antibody-like single chain proteins
WO2011051466A1 (en) 2009-11-02 2011-05-05 Novartis Ag Anti-idiotypic fibronectin-based binding molecules and uses thereof
NZ600361A (en) 2009-12-21 2014-06-27 Ambrx Inc Modified bovine somatotropin polypeptides and their uses
EP2516455A4 (de) 2009-12-21 2013-05-22 Ambrx Inc Modifizierte schweine-somatotropin-polypeptide und deren verwendungen
WO2011092233A1 (en) 2010-01-29 2011-08-04 Novartis Ag Yeast mating to produce high-affinity combinations of fibronectin-based binders
EP2605789B1 (de) 2010-08-17 2019-06-05 Ambrx, Inc. Modifizierte relaxinpolypeptide und ihre verwendungen
US9567386B2 (en) 2010-08-17 2017-02-14 Ambrx, Inc. Therapeutic uses of modified relaxin polypeptides
CN103261222B (zh) 2010-09-10 2017-07-28 医疗免疫有限公司 抗体衍生物
AR083006A1 (es) 2010-09-23 2013-01-23 Lilly Co Eli Formulaciones para el factor estimulante de colonias de granulocitos (g-csf) bovino y variantes de las mismas
WO2012097333A2 (en) 2011-01-14 2012-07-19 Redwood Bioscience, Inc. Aldehyde-tagged immunoglobulin polypeptides and method of use thereof
SG11201502216UA (en) 2012-09-24 2015-04-29 Medimmune Ltd Cell lines
CN102946253B (zh) * 2012-10-23 2016-06-08 保定市三川电气有限责任公司 数据采样方法与系统及其在参数辨识中的应用方法与系统
UY35368A (es) 2013-03-08 2014-10-31 Irm Llc Péptidos y composiciones para el tratamiento de daño articular
WO2014160463A1 (en) 2013-03-13 2014-10-02 The United States Of America, As Represented By The Secretary, Department Of Health & Human Services Prefusion rsv f proteins and their use
US10450361B2 (en) 2013-03-15 2019-10-22 Momenta Pharmaceuticals, Inc. Methods related to CTLA4-Fc fusion proteins
ES2708759T3 (es) 2013-05-13 2019-04-11 Momenta Pharmaceuticals Inc Procedimientos para el tratamiento de la neurodegeneración
WO2015009948A1 (en) 2013-07-19 2015-01-22 Regents Of The University Of California Milk fat globule epidermal growth factor 8 regulates fatty acid uptake
EP3058084A4 (de) 2013-10-16 2017-07-05 Momenta Pharmaceuticals, Inc. Sialylierte glycoproteine
US11504346B2 (en) 2013-11-03 2022-11-22 Arizona Board Of Regents On Behalf Of The University Of Arizona Redox-activated pro-chelators
US12383518B2 (en) 2013-11-03 2025-08-12 Arizona Board Of Regents On Behalf Of The University Of Arizona Disulfide-masked pro-chelator compositions and methods of use
US10501734B2 (en) * 2014-02-06 2019-12-10 Yale University Compositions and methods of use thereof for making polypeptides with many instances of nonstandard amino acids
US9616114B1 (en) 2014-09-18 2017-04-11 David Gordon Bermudes Modified bacteria having improved pharmacokinetics and tumor colonization enhancing antitumor activity
KR102854572B1 (ko) 2014-10-24 2025-09-02 브리스톨-마이어스 스큅 컴퍼니 변형된 fgf-21 폴리펩티드 및 그의 용도
US10676723B2 (en) 2015-05-11 2020-06-09 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
WO2017117539A1 (en) 2015-12-30 2017-07-06 Northwestern University Cell-free glycoprotein synthesis (cfgps) in prokaryotic cell lysates enriched with components for glycosylation
JP2019515677A (ja) 2016-04-26 2019-06-13 アール．ピー．シェーラー テクノロジーズ エルエルシー 抗体複合体ならびにそれを作製および使用する方法
US10829795B2 (en) 2016-07-14 2020-11-10 Northwestern University Method for rapid in vitro synthesis of glycoproteins via recombinant production of N-glycosylated proteins in prokaryotic cell lysates
CN106316920B (zh) * 2016-07-23 2018-12-04 常州大学 一种新型吲哚甘氨酸衍生物的合成方法
KR20190084287A (ko) 2016-11-14 2019-07-16 노파르티스 아게 연골 손상 및 관절염 치료 방법 및 조성물
CN109996809A (zh) 2016-11-14 2019-07-09 诺华股份有限公司 与促融合蛋白minion相关的组合物、方法和治疗用途
US11180535B1 (en) 2016-12-07 2021-11-23 David Gordon Bermudes Saccharide binding, tumor penetration, and cytotoxic antitumor chimeric peptides from therapeutic bacteria
US11129906B1 (en) 2016-12-07 2021-09-28 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
US11951165B2 (en) 2016-12-30 2024-04-09 Vaxcyte, Inc. Conjugated vaccine carrier proteins
MX2019007910A (es) 2016-12-30 2019-12-05 Sutrovax Inc Conjugados de polipeptido-antigeno con aminoacidos no naturales.
BR112019016139A2 (pt) 2017-02-08 2020-04-07 Bristol-Myers Squibb Company polipeptídio de relaxina modificada compreendendo um melhorador farmacocinético e usos do mesmo
US11898187B2 (en) 2017-08-15 2024-02-13 Northwestern University Protein glycosylation sites by rapid expression and characterization of N-glycosyltransferases
US11530432B2 (en) 2018-03-19 2022-12-20 Northwestern University Compositions and methods for rapid in vitro synthesis of bioconjugate vaccines in vitro via production and N-glycosylation of protein carriers in detoxified prokaryotic cell lysates
US11725224B2 (en) 2018-04-16 2023-08-15 Northwestern University Methods for co-activating in vitro non-standard amino acid (nsAA) incorporation and glycosylation in crude cell lysates
US12404512B2 (en) 2018-05-01 2025-09-02 Ambrx, Inc. Method for optimizing antibody expression
US12098433B2 (en) 2018-08-03 2024-09-24 Northwestern University On demand, portable, cell-free molecular sensing platform
WO2020056066A1 (en) 2018-09-11 2020-03-19 Ambrx, Inc. Interleukin-2 polypeptide conjugates and their uses
AU2019361206A1 (en) 2018-10-19 2021-06-03 Ambrx, Inc. Interleukin-10 polypeptide conjugates, dimers thereof, and their uses
JP7644454B2 (ja) 2019-01-11 2025-03-12 ノースウェスタン ユニバーシティ 原核細胞溶解物におけるバイオコンジュゲートワクチンの合成
WO2020185451A2 (en) 2019-03-04 2020-09-17 Northwestern University Riboswitch-based fluoride sensing in cell-free extract
TW202128996A (zh) * 2019-10-10 2021-08-01 美商史基普研究協會 用於活體內合成非天然多肽的組合物及方法
US12226410B2 (en) 2019-10-18 2025-02-18 Northwestern University Methods for enhancing cellular clearance of pathological molecules via activation of the cellular protein ykt6
CN120682337A (zh) 2020-03-11 2025-09-23 北京泰德制药股份有限公司 白介素-2多肽偶联物及其使用方法
US12139921B2 (en) * 2021-11-18 2024-11-12 Jeff Sellers Pole cover assembly for a street pole, sign and other objects for improving visibility and aesthetic appeal

Family Cites Families (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991016449A1 (en) 1990-04-16 1991-10-31 The Trustees Of The University Of Pennsylvania Saccharide compositions, methods and apparatus for their synthesis
US5369017A (en) 1994-02-04 1994-11-29 The Scripps Research Institute Process for solid phase glycopeptide synthesis
US6465612B1 (en) 1998-09-23 2002-10-15 The Regents Of The University Of California Synthetic peptides, conjugation reagents and methods
JP2005502322A (ja) * 2001-04-19 2005-01-27 ザ スクリップス リサーチ インスティテュート 非天然アミノ酸のインビボ組込み
CA2462930C (en) 2001-10-10 2012-07-10 Shawn De Frees Remodeling and glycoconjugation of peptides
JP4444113B2 (ja) * 2002-10-16 2010-03-31 ザ スクリップス リサーチ インスティテュート ケトアミノ酸の部位特異的蛋白質組込み方法
EP1558747B1 (de) * 2002-10-16 2009-10-14 The Scripps Research Institute Glycoproteinsynthese
MXPA05006836A (es) * 2002-12-22 2005-08-16 Scripps Research Inst Colecciones ordenadas de proteinas.
CN103215227B (zh) * 2003-04-17 2015-09-16 斯克利普斯研究院 扩展真核生物遗传密码
JP5275566B2 (ja) 2003-06-18 2013-08-28 ザ スクリプス リサーチ インスティチュート 反応性非天然アミノ酸遺伝コード付加
WO2005007624A2 (en) * 2003-07-07 2005-01-27 The Scripps Research Institute Compositions of orthogonal glutamyl-trna and aminoacyl trna synthetase pairs and uses thereof
EP1704242A4 (de) * 2003-07-07 2008-06-18 Scripps Research Inst ZUSAMMENSETZUNGEN MIT PAAREN VON ORTHOGONALER LEUCYL-tRNA UND AMINOACYL-tRNA-SYNTHETASE UND VERWENDUNGEN DAVON
ES2301099T3 (es) * 2003-07-07 2008-06-16 The Scripps Research Institute Composiciones de parejas ortogonales lisil-arnt y aminoacil-arnt sintetasa y usos de las mismas.
WO2005038002A2 (en) * 2003-10-14 2005-04-28 The Scripps Research Institute Site-specific incorporation of redox active amino acids into proteins
CA2549830A1 (en) * 2003-12-18 2006-01-05 The Scripps Research Institute Selective incorporation of 5-hyroxytryptophan into proteins in mammalian cells
MXPA06013586A (es) * 2004-05-25 2007-11-09 Scripss Res Inst Incorporacion especifica del sitio de aminoacidos no naturales que contienen atomos pesados en proteinas para la determinacion de la estructura cristalina.
US20060134746A1 (en) * 2004-09-21 2006-06-22 The Scripps Research Institute Adding photoregulated amino acids to the genetic code
CN101151366B (zh) * 2004-09-21 2012-12-19 斯克利普斯研究院 将炔基氨基酸体内掺入真细菌的蛋白
EP1794312A4 (de) * 2004-09-22 2009-08-12 Scripps Research Inst Ortsspezifische etikettierung von proteinen für nmr-studien
EP1809758A4 (de) * 2004-10-20 2009-04-01 Scripps Research Inst Stellenspezifischer in-vivo-einbau von n-acetylgalactosamin-aminosäuren in eubakterien
US8216804B2 (en) * 2004-10-27 2012-07-10 The Scripps Research Institute Orthogonal translation components for the in vivo incorporation of unnatural amino acids
AU2006304110B2 (en) * 2005-10-12 2012-02-02 The Scripps Research Institute Selective posttranslational modification of phage-displayed polypeptides
CN101400796B (zh) * 2006-03-09 2013-03-27 斯克利普斯研究院 在真细菌宿主细胞内表达正交翻译组分的系统
RU2464315C2 (ru) * 2006-03-16 2012-10-20 Зе Скрипс Ресеч Инститьют Генетически запрограммированная экспрессия белков, содержащих не встречающуюся в природе аминокислоту фенилселеноцистеин
US7807411B2 (en) * 2006-05-23 2010-10-05 The Scripps Research Institute Genetically encoded fluorescent coumarin amino acids
EP2052083B1 (de) * 2006-09-21 2014-01-08 The Scripps Research Institute Genetisch programmierte expression von selektiv sulfatierten proteinen in eubakterien
EP2069481A4 (de) * 2006-10-18 2010-01-06 Scripps Research Inst Genetischer einbau unnatürlicher aminosäuren in proteine in säugerzellen
US20090197339A1 (en) * 2008-12-10 2009-08-06 The Scripps Research Institute In vivo unnatural amino acid expression in the methylotrophic yeast pichia pastoris

Also Published As

Publication number Publication date
IL196909A0 (en) 2011-07-31
EP1558747B1 (de) 2009-10-14
IL196908A0 (en) 2011-07-31
US20050181471A1 (en) 2005-08-18
US20050266526A1 (en) 2005-12-01
US20070184517A1 (en) 2007-08-09
IL196907A0 (en) 2011-07-31
US20050186656A1 (en) 2005-08-25
AU2003286436A1 (en) 2004-05-04
ES2333422T3 (es) 2010-02-22
WO2004035605A2 (en) 2004-04-29
HK1074855A1 (en) 2006-01-13
DE60329700D1 (en) 2009-11-26
US20050186657A1 (en) 2005-08-25
IL196906A0 (en) 2011-07-31
IL196905A (en) 2012-08-30
MXPA05003978A (es) 2005-06-22
US7262040B2 (en) 2007-08-28
EP1558747A4 (de) 2007-07-18
US6927042B2 (en) 2005-08-09
US7238510B2 (en) 2007-07-03
US7217809B2 (en) 2007-05-15
US7129333B2 (en) 2006-10-31
JP2006507358A (ja) 2006-03-02
US7183082B2 (en) 2007-02-27
SG160203A1 (en) 2010-04-29
WO2004035605A3 (en) 2005-05-12
US20040138106A1 (en) 2004-07-15
US20070172915A1 (en) 2007-07-26
US20050209133A1 (en) 2005-09-22
US7199222B2 (en) 2007-04-03
US20080213828A1 (en) 2008-09-04
CA2500653A1 (en) 2004-04-29
IL196904A0 (en) 2011-07-31
US20050233412A1 (en) 2005-10-20
AU2003286436B2 (en) 2010-08-19
US20100009425A1 (en) 2010-01-14
US7560535B2 (en) 2009-07-14
EP1558747A2 (de) 2005-08-03
US7834159B2 (en) 2010-11-16
US7825226B2 (en) 2010-11-02
KR20050073559A (ko) 2005-07-14
IL196905A0 (en) 2011-07-31
JP4752001B2 (ja) 2011-08-17

Similar Documents

Publication Publication Date Title
ATE445709T1 (de) Glycoproteinsynthese
WO2006045116A3 (en) In vivo site-specific incorporation of n-acetyl-galactosamine amino acids in eubacteria
BG108492A (bg) Използване на въглероден окис за подобряване на резултатите при трансплантация на тъкани и органи и потискане на апоптозата
EA200701377A1 (ru) Имплантируемый биоматериал и способ его получения
WO2007047829A3 (en) Novel heterodimeric proteins and uses thereof
IL225738A0 (en) A vertebrate cell or cell line that contains amino acyl tRNA synthetase
WO2003060090A3 (en) Novel cytokine zcytor17 ligand
DK1448229T3 (da) Farmaceutisk sammensætning til at inducere et immunrespons hos et menneske eller et dyr
WO2020167455A3 (en) Platform for producing glycoproteins, identifying glycosylation pathways
WO2008030613A3 (en) Hybrid suppressor trna for vertebrate cells
GB0208041D0 (en) Method of culturing animal cells
PT1276763E (pt) Proteinas anti-congelantes sua producao e utilizacao
WO2004081033A3 (en) Synthesis of proteins by cell-free protein expression
EP1641813A4 (de) Verfahren zur herstellung von molekülen mit verminderter immunogenität
PL1817330T3 (pl) Pochodne alergenów białkowych
NO20020329L (no) Antitumor-antistoffer, proteiner og anvendelse derav
AU2001217678A1 (en) In vitro protein synthesis using glycolytic intermediates as an energy source
TW200735781A (en) Food product
WO2002074921A3 (en) Methods for isolating proteins expressed by dendritic cells
ATE329600T1 (de) Verwendung von tagatose zur verbesserung wichtiger blutfaktoren
AU2003277694A1 (en) Porcine uroplakin ii promoter and the production method of useful proteins using said promoter
ATE320499T1 (de) Optimierte proteinsynthese
WO2001044490A3 (en) A NOVEL METHOD TO PRODUCE HUMAN NATURAL INTERFERON-$g(a)
EA200101093A1 (ru) Экспрессия гепарин-связывающего протеина в рекомбинантных клетках млекопитающих
TW200616658A (en) Endogenously-formed conjugate of albumin

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties