ATE412761T1 - Methode um rekombinante proteine in cho zellen zu exprimieren - Google Patents
Methode um rekombinante proteine in cho zellen zu exprimierenInfo
- Publication number
- ATE412761T1 ATE412761T1 AT03765075T AT03765075T ATE412761T1 AT E412761 T1 ATE412761 T1 AT E412761T1 AT 03765075 T AT03765075 T AT 03765075T AT 03765075 T AT03765075 T AT 03765075T AT E412761 T1 ATE412761 T1 AT E412761T1
- Authority
- AT
- Austria
- Prior art keywords
- cho cells
- recombinant proteins
- express recombinant
- express
- enhancing
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/10—Plasmid DNA
- C12N2800/108—Plasmid DNA episomal vectors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/42—Vector systems having a special element relevant for transcription being an intron or intervening sequence for splicing and/or stability of RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2840/00—Vectors comprising a special translation-regulating system
- C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Biophysics (AREA)
- Mycology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GBGB0216648.6A GB0216648D0 (en) | 2002-07-18 | 2002-07-18 | Method of expressing recombinant protein in CHO cells |
PCT/EP2003/007946 WO2004009823A1 (en) | 2002-07-18 | 2003-07-21 | Method of expressing recombinant protein in cho cells |
Publications (1)
Publication Number | Publication Date |
---|---|
ATE412761T1 true ATE412761T1 (de) | 2008-11-15 |
Family
ID=9940670
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AT03765075T ATE412761T1 (de) | 2002-07-18 | 2003-07-21 | Methode um rekombinante proteine in cho zellen zu exprimieren |
Country Status (14)
Country | Link |
---|---|
US (3) | US20060003405A1 (de) |
EP (1) | EP1525320B1 (de) |
CN (1) | CN1296486C (de) |
AT (1) | ATE412761T1 (de) |
AU (2) | AU2003251434B2 (de) |
CA (2) | CA2489016C (de) |
DE (1) | DE60324430D1 (de) |
DK (1) | DK1525320T3 (de) |
ES (1) | ES2314248T3 (de) |
GB (1) | GB0216648D0 (de) |
HK (1) | HK1081595A1 (de) |
PT (1) | PT1525320E (de) |
SI (1) | SI1525320T1 (de) |
WO (1) | WO2004009823A1 (de) |
Families Citing this family (82)
Publication number | Priority date | Publication date | Assignee | Title |
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GB0216648D0 (en) * | 2002-07-18 | 2002-08-28 | Lonza Biologics Plc | Method of expressing recombinant protein in CHO cells |
GB0319451D0 (en) * | 2003-08-19 | 2003-09-17 | Lonza Biologics Plc | Tropolone assay |
EP2404935A1 (de) | 2004-03-30 | 2012-01-11 | Glaxo Group Limited | Immunglobuline gegen menschlisches OSM |
TWI307630B (en) | 2004-07-01 | 2009-03-21 | Glaxo Group Ltd | Immunoglobulins |
AU2006237193B2 (en) * | 2005-04-22 | 2012-02-02 | Lonza Biologics Plc. | Mammalian expression vector comprising the mCMV promoter and first intron of hCMV major immediate early gene |
US20090215165A1 (en) * | 2005-05-20 | 2009-08-27 | James Rance | High-level expression of recombinant antibody in a mammalian host cell |
BRPI0613770A2 (pt) | 2005-07-22 | 2009-05-19 | Y S Therapeutics Co Ltd | anticorpos anti-cd26 e métodos de uso destes |
US9884899B2 (en) | 2007-07-06 | 2018-02-06 | Promedior, Inc. | Methods for treating fibrosis using CRP antagonists |
PL3327026T3 (pl) | 2007-07-09 | 2020-02-28 | Genentech, Inc. | Zapobieganie redukcji wiązań disiarczkowych podczas rekombinacyjnego wytwarzania polipeptydów |
AR074777A1 (es) | 2008-12-19 | 2011-02-09 | Glaxo Group Ltd | Proteinas de union a antigeno |
PT2405928T (pt) | 2009-03-11 | 2017-02-07 | Promedior Inc | Métodos de tratamento e de diagnóstico para patologias de hipersensibilidade |
DK2405929T3 (en) | 2009-03-11 | 2018-08-27 | Promedior Inc | SAP polypeptide for use in the treatment of autoimmune disorders and graft-versus-host disease |
UA110323C2 (en) | 2009-06-04 | 2015-12-25 | Promedior Inc | Derivative of serum amyloid p and their receipt and application |
DK2987803T3 (en) | 2009-06-17 | 2018-12-17 | Promedior Inc | SAP variants and their application |
KR101822205B1 (ko) | 2009-08-11 | 2018-01-25 | 제넨테크, 인크. | 글루타민-비함유 세포 배양 배지에서의 단백질의 생성 |
WO2011080050A2 (en) | 2009-12-11 | 2011-07-07 | Novartis Ag | Binding molecules |
EP2528947A4 (de) | 2010-01-28 | 2013-09-18 | Glaxo Group Ltd | Cd127-bindende proteine |
MY171234A (en) | 2010-02-24 | 2019-10-04 | Immunogen Inc | Folate receptor 1 antibodies and immunoconjugates and uses thereof |
UA108227C2 (xx) | 2010-03-03 | 2015-04-10 | Антигензв'язуючий білок | |
RU2610662C9 (ru) | 2010-03-12 | 2017-07-24 | Иммьюноджен, Инк. | Cd37-связывающие молекулы и их иммуноконъюгаты |
AR081556A1 (es) | 2010-06-03 | 2012-10-03 | Glaxo Group Ltd | Proteinas de union al antigeno humanizadas |
EP2596027A1 (de) | 2010-07-22 | 2013-05-29 | GlaxoSmithKline Biologicals S.A. | Neuartige antigenbindende proteine |
WO2012019024A2 (en) | 2010-08-04 | 2012-02-09 | Immunogen, Inc. | Her3-binding molecules and immunoconjugates thereof |
AU2012275133B2 (en) | 2011-06-30 | 2017-08-17 | Genzyme Corporation | Inhibitors of T-cell activation |
RU2014101669A (ru) | 2011-07-15 | 2015-09-20 | Онкомед Фармасьютикалс, Инк. | Агенты, связывающие белки r-спондины (rspo), и способы их применения |
EP2734232B1 (de) | 2011-07-19 | 2017-11-01 | Philogen S.p.A. | Sequentielle anti-ctla4 und zielgerichtete il-2 therapie |
UY34317A (es) | 2011-09-12 | 2013-02-28 | Genzyme Corp | Anticuerpo antireceptor de célula T (alfa)/ß |
CN107722122B (zh) | 2011-09-23 | 2022-05-27 | 昂考梅德药品有限公司 | Vegf/dll4结合剂及其应用 |
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EP2858673A4 (de) | 2012-06-06 | 2016-06-22 | Oncomed Pharm Inc | Bindungsmittel zur modulation des hippo-pfads und verwendungen davon |
EP2674495A1 (de) * | 2012-06-14 | 2013-12-18 | Sanofi | CHO-Expressionssystem |
NZ704269A (en) | 2012-07-13 | 2016-05-27 | Oncomed Pharm Inc | Rspo3 binding agents and uses thereof |
NZ726258A (en) | 2012-08-31 | 2019-07-26 | Immunogen Inc | Antibodies and uses thereof to detect folate receptor 1 |
EP2711426B1 (de) | 2012-09-24 | 2015-04-08 | Lonza Biologics plc | Expressionsvektoren mit chimärem Cytomegalieviruspromotor und Verstärkersequenzen |
KR20150090919A (ko) | 2012-12-04 | 2015-08-06 | 온코메드 파마슈티칼스, 인크. | 결합제를 사용한 면역요법 |
WO2014100073A2 (en) * | 2012-12-21 | 2014-06-26 | Merck Sharp & Dohme Corp. | Expression vectors for recombinant protein production in mammalian cells |
KR20230145514A (ko) | 2013-08-30 | 2023-10-17 | 이뮤노젠 아이엔씨 | 엽산 수용체 1의 검출을 위한 항체 및 분석 |
US10203327B2 (en) | 2013-11-05 | 2019-02-12 | Novartis Ag | Organic compounds |
TW201610168A (zh) | 2013-12-02 | 2016-03-16 | 安可美德藥物股份有限公司 | 與Wnt途徑抑制劑有關之預測性生物標記之鑑別 |
EP3099713B1 (de) | 2014-02-02 | 2020-01-15 | MedImmune Limited | Chimäres proteins bestehtend aus einer ngf antagonist und einer tnfa antagonist domaine |
US8961992B1 (en) * | 2014-04-02 | 2015-02-24 | Tunitas Therapeutics, Inc. | Epsigam fusion protein |
DK3128997T3 (da) | 2014-04-08 | 2020-08-24 | Boston Pharmaceuticals Inc | Bindingmolekyler specifikke for il-21 og anvendelser deraf |
CA2943299A1 (en) | 2014-04-11 | 2015-10-15 | Medimmune, Llc | Bispecific her2 antibodies |
WO2015165480A1 (en) | 2014-04-30 | 2015-11-05 | Institute For Research In Biomedicine | Human cytomegalovirus vaccine compositions and method of producing the same |
CN106456734A (zh) | 2014-05-29 | 2017-02-22 | 免疫医疗有限责任公司 | Ox40l融合蛋白及其用途 |
AU2015289386A1 (en) * | 2014-07-18 | 2017-03-09 | Celltheon Corporation | Methods and compositions for expression of polypeptides in a cell |
CN104195173B (zh) * | 2014-09-02 | 2017-03-08 | 北京比洋生物技术有限公司 | 具有双表达盒的谷氨酰胺合成酶高效表达载体 |
TW201619200A (zh) | 2014-10-10 | 2016-06-01 | 麥迪紐有限責任公司 | 人類化抗-ox40抗體及其用途 |
WO2016059602A2 (en) | 2014-10-16 | 2016-04-21 | Glaxo Group Limited | Methods of treating cancer and related compositions |
DK3218406T3 (da) | 2014-11-10 | 2021-06-21 | Medimmune Ltd | Bindingsmolekyler, der er specifikke for cd73, og anvendelser deraf |
US20160129108A1 (en) | 2014-11-11 | 2016-05-12 | Medimmune Limited | Therapeutic combinations comprising anti-cd73 antibodies and uses thereof |
CN104561091A (zh) * | 2014-12-17 | 2015-04-29 | 华南理工大学 | 一种光学分析检测cho细胞增殖的方法及应用 |
CN105779501A (zh) * | 2014-12-26 | 2016-07-20 | 上海药明康德新药开发有限公司 | 运用cho宿主细胞进行重组蛋白的瞬时表达生产的方法 |
CN105802995A (zh) * | 2014-12-31 | 2016-07-27 | 上海复宏汉霖生物技术有限公司 | 一种利用cho细胞生产重组蛋白的基因表达系统及真核表达载体 |
CN107206079A (zh) | 2015-01-09 | 2017-09-26 | 免疫医疗有限责任公司 | 检测人dpp‑4的测定法 |
CN107849111B (zh) | 2015-05-04 | 2022-02-22 | Vcn生物科学有限公司 | 在免疫显性腺病毒抗原表位中具有突变的溶瘤腺病毒及其在癌症治疗中的用途 |
EP3313884B9 (de) | 2015-06-29 | 2021-05-19 | ImmunoGen, Inc. | Anti-cd123-antikörper und wirkstoffe und derivate davon |
MX2018001787A (es) | 2015-08-12 | 2018-06-06 | Medimmune Ltd | Proteinas de fusion del ligando de la proteina relacionada con el receptor de factor de necrosis tumoral inducido por glucorticoides (gitrl) y usos de las mismas. |
JP6967003B2 (ja) | 2015-09-23 | 2021-11-17 | メレオ バイオファーマ 5 インコーポレイテッド | がんの処置のための方法および組成物 |
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WO2017083451A1 (en) | 2015-11-10 | 2017-05-18 | Medimmune, Llc | Binding molecules specific for asct2 and uses thereof |
AU2017230091B2 (en) | 2016-03-10 | 2022-04-07 | Acceleron Pharma Inc. | Activin type 2 receptor binding proteins and uses thereof |
US10913783B2 (en) | 2016-04-15 | 2021-02-09 | H. Lundbeck A/S | Humanized anti-PACAP antibodies and uses thereof |
CA3025377A1 (en) | 2016-06-02 | 2017-12-07 | Abbvie Inc. | Glucocorticoid receptor agonist and immunoconjugates thereof |
AU2017341028B2 (en) | 2016-10-07 | 2023-12-21 | Genzyme Corporation | Early post-transfection isolation of cells (EPIC) for biologics production |
US11186626B2 (en) | 2016-10-11 | 2021-11-30 | Integrated Biotherapeutics, Inc. | Broadly neutralizing antibody targeting the ebolavirus glycoprotein internal fusion loop |
CN110662770A (zh) | 2016-11-23 | 2020-01-07 | 比奥维拉迪维治疗股份有限公司 | 结合凝血因子ix和凝血因子x的双特异性抗体 |
CN107973841B (zh) * | 2016-12-23 | 2022-03-25 | 浙江海隆生物科技有限公司 | Cho细胞表达的重组牛病毒性腹泻病毒e2蛋白及亚单位疫苗的制备方法和应用 |
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GB201703418D0 (en) | 2017-03-03 | 2017-04-19 | Ge Healthcare Bio Sciences Ab | Method for cell line development |
CN110785494A (zh) | 2017-06-16 | 2020-02-11 | 隆萨有限公司 | 用于生产生物制品的通用自调节哺乳动物细胞系平台 |
RU2020117698A (ru) | 2017-12-01 | 2022-01-04 | Эббви Инк. | Агонист глюкокортикоидного рецептора и его иммуноконъюгаты |
JP2022516505A (ja) | 2018-12-28 | 2022-02-28 | スパークス・セラピューティクス・インコーポレイテッド | 癌および他の疾患の治療のための、クローディン18.2に特異的な結合分子、その組成物および方法 |
AU2020253455A1 (en) | 2019-04-03 | 2021-11-04 | Genzyme Corporation | Anti-alpha beta TCR binding polypeptides with reduced fragmentation |
KR20220037435A (ko) | 2019-06-18 | 2022-03-24 | 유나이티드 쎄러퓨틱스 코포레이션 | 이식용 장기의 미토콘드리아 처리 |
CN113005140B (zh) * | 2019-12-19 | 2024-03-15 | 鲁南制药集团股份有限公司 | 一种具有双表达盒的gs表达载体及其应用 |
WO2021251340A1 (ja) | 2020-06-08 | 2021-12-16 | ワイズ・エー・シー株式会社 | 抗癌剤への抵抗性改善剤 |
BR112023004840A2 (pt) | 2020-09-28 | 2023-04-18 | Medimmune Ltd | Compostos e métodos para tratamento de dor |
JP2022184105A (ja) | 2021-05-31 | 2022-12-13 | ワイズ・エー・シー株式会社 | 抗cd26抗体と免疫チェックポイント阻害剤との併用療法 |
US11807685B2 (en) | 2021-08-05 | 2023-11-07 | The Uab Research Foundation | Anti-CD47 antibody and uses thereof |
WO2023064947A1 (en) | 2021-10-15 | 2023-04-20 | Regenxbio Inc. | Antibodies and methods of using thereof |
US20240101691A1 (en) | 2022-09-21 | 2024-03-28 | Sanofi Biotechnology | Humanized anti-il-1r3 antibody and methods of use |
Family Cites Families (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE2944850A1 (de) * | 1979-11-07 | 1981-05-27 | Bayer Ag, 5090 Leverkusen | Mittel zur regulierung des pflanzenwachstums, deren herstellung und deren verwendung |
US4968615A (en) | 1985-12-18 | 1990-11-06 | Ciba-Geigy Corporation | Deoxyribonucleic acid segment from a virus |
US4963481A (en) * | 1985-12-18 | 1990-10-16 | Genetics Institute Inc | Promoter system |
GB8601597D0 (en) | 1986-01-23 | 1986-02-26 | Wilson R H | Nucleotide sequences |
US5891693A (en) * | 1990-01-25 | 1999-04-06 | Alusuisse Holdings A.G. | Recombinant DNA methods vectors and host cells |
IT1262545B (it) | 1993-10-25 | 1996-07-02 | Menarini Ricerche Sud S P A A | Sistema di espressione per linee cellulari eucariotiche |
DK0731845T3 (da) * | 1993-12-23 | 2003-10-20 | Merck & Co Inc | Homologt rekombinant antistofekspressionssystem til murine celler |
AU684134B2 (en) | 1994-02-07 | 1997-12-04 | Qiagen Gmbh | Nucleic acid transfection efficiency increase by use of isopropanol in aqueous solutions |
GB0216648D0 (en) * | 2002-07-18 | 2002-08-28 | Lonza Biologics Plc | Method of expressing recombinant protein in CHO cells |
ATE399872T1 (de) | 2003-03-11 | 2008-07-15 | Serono Lab | Expressionvektoren, die den promotor des ie2-gens des maus cytomegalovirus enthalten |
-
2002
- 2002-07-18 GB GBGB0216648.6A patent/GB0216648D0/en not_active Ceased
-
2003
- 2003-07-21 SI SI200331477T patent/SI1525320T1/sl unknown
- 2003-07-21 AT AT03765075T patent/ATE412761T1/de active
- 2003-07-21 ES ES03765075T patent/ES2314248T3/es not_active Expired - Lifetime
- 2003-07-21 EP EP03765075A patent/EP1525320B1/de not_active Expired - Lifetime
- 2003-07-21 PT PT03765075T patent/PT1525320E/pt unknown
- 2003-07-21 AU AU2003251434A patent/AU2003251434B2/en not_active Expired
- 2003-07-21 US US10/521,768 patent/US20060003405A1/en not_active Abandoned
- 2003-07-21 CA CA2489016A patent/CA2489016C/en not_active Expired - Lifetime
- 2003-07-21 CA CA2672809A patent/CA2672809C/en not_active Expired - Lifetime
- 2003-07-21 CN CNB038169142A patent/CN1296486C/zh not_active Expired - Lifetime
- 2003-07-21 DK DK03765075T patent/DK1525320T3/da active
- 2003-07-21 WO PCT/EP2003/007946 patent/WO2004009823A1/en active Application Filing
- 2003-07-21 DE DE60324430T patent/DE60324430D1/de not_active Expired - Lifetime
-
2006
- 2006-02-10 HK HK06101786A patent/HK1081595A1/xx not_active IP Right Cessation
-
2007
- 2007-12-21 US US11/962,261 patent/US7932087B2/en not_active Expired - Lifetime
-
2009
- 2009-02-19 AU AU2009200665A patent/AU2009200665B2/en not_active Expired
-
2010
- 2010-12-10 US US12/964,918 patent/US20110104745A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
CA2672809C (en) | 2013-01-08 |
CN1668749A (zh) | 2005-09-14 |
US20080138902A1 (en) | 2008-06-12 |
US7932087B2 (en) | 2011-04-26 |
AU2009200665A1 (en) | 2009-03-12 |
DK1525320T3 (da) | 2009-02-23 |
CN1296486C (zh) | 2007-01-24 |
GB0216648D0 (en) | 2002-08-28 |
CA2672809A1 (en) | 2004-01-29 |
AU2009200665B2 (en) | 2012-08-16 |
ES2314248T3 (es) | 2009-03-16 |
HK1081595A1 (en) | 2006-05-19 |
EP1525320A1 (de) | 2005-04-27 |
SI1525320T1 (sl) | 2009-02-28 |
EP1525320B1 (de) | 2008-10-29 |
CA2489016A1 (en) | 2004-01-29 |
DE60324430D1 (de) | 2008-12-11 |
WO2004009823A1 (en) | 2004-01-29 |
AU2003251434A1 (en) | 2004-02-09 |
US20060003405A1 (en) | 2006-01-05 |
US20110104745A1 (en) | 2011-05-05 |
PT1525320E (pt) | 2009-01-23 |
CA2489016C (en) | 2012-01-17 |
AU2003251434B2 (en) | 2009-03-05 |
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