ATE320508T1 - Oligonukleotide mit pyrazol(3,4-d) pyrimidin zur hybridisierung und unterscheidung von fehlpaarungen - Google Patents

Oligonukleotide mit pyrazol(3,4-d) pyrimidin zur hybridisierung und unterscheidung von fehlpaarungen

Info

Publication number
ATE320508T1
ATE320508T1 AT99916394T AT99916394T ATE320508T1 AT E320508 T1 ATE320508 T1 AT E320508T1 AT 99916394 T AT99916394 T AT 99916394T AT 99916394 T AT99916394 T AT 99916394T AT E320508 T1 ATE320508 T1 AT E320508T1
Authority
AT
Austria
Prior art keywords
oligonucleotides
hybridization
pyrimidine
pyrazolo
pyrazole
Prior art date
Application number
AT99916394T
Other languages
English (en)
Inventor
Rich B Meyer Jr
Irina A Afonina
Igor V Kutyavin
Original Assignee
Epoch Biosciences Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Epoch Biosciences Inc filed Critical Epoch Biosciences Inc
Application granted granted Critical
Publication of ATE320508T1 publication Critical patent/ATE320508T1/de

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6832—Enhancement of hybridisation reaction

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT99916394T 1998-04-03 1999-04-05 Oligonukleotide mit pyrazol(3,4-d) pyrimidin zur hybridisierung und unterscheidung von fehlpaarungen ATE320508T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US09/054,830 US6127121A (en) 1998-04-03 1998-04-03 Oligonucleotides containing pyrazolo[3,4-D]pyrimidines for hybridization and mismatch discrimination

Publications (1)

Publication Number Publication Date
ATE320508T1 true ATE320508T1 (de) 2006-04-15

Family

ID=21993802

Family Applications (1)

Application Number Title Priority Date Filing Date
AT99916394T ATE320508T1 (de) 1998-04-03 1999-04-05 Oligonukleotide mit pyrazol(3,4-d) pyrimidin zur hybridisierung und unterscheidung von fehlpaarungen

Country Status (8)

Country Link
US (4) US6127121A (de)
EP (1) EP1068358B1 (de)
JP (1) JP4558932B2 (de)
AT (1) ATE320508T1 (de)
AU (1) AU759944B2 (de)
CA (1) CA2327547C (de)
DE (1) DE69930379T2 (de)
WO (1) WO1999051775A1 (de)

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AU3472499A (en) 1999-10-25
AU759944B2 (en) 2003-05-01
WO1999051775A1 (en) 1999-10-14
EP1068358A1 (de) 2001-01-17
CA2327547A1 (en) 1999-10-14
JP2002510507A (ja) 2002-04-09
US20020146690A1 (en) 2002-10-10
US6127121A (en) 2000-10-03
US20100228017A1 (en) 2010-09-09
DE69930379T2 (de) 2006-10-05
CA2327547C (en) 2009-10-27
DE69930379D1 (de) 2006-05-11
US6485906B2 (en) 2002-11-26
JP4558932B2 (ja) 2010-10-06
US20030143602A1 (en) 2003-07-31

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