AR119721A2 - Elementos de regulación de plantas y sus usos - Google Patents
Elementos de regulación de plantas y sus usosInfo
- Publication number
- AR119721A2 AR119721A2 ARP200101167A ARP200101167A AR119721A2 AR 119721 A2 AR119721 A2 AR 119721A2 AR P200101167 A ARP200101167 A AR P200101167A AR P200101167 A ARP200101167 A AR P200101167A AR 119721 A2 AR119721 A2 AR 119721A2
- Authority
- AR
- Argentina
- Prior art keywords
- elements
- dna molecules
- present
- plant cells
- plant
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
- C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
- C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
- C12N15/8223—Vegetative tissue-specific promoters
- C12N15/8225—Leaf-specific, e.g. including petioles, stomata
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
- C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
- C12N15/8223—Vegetative tissue-specific promoters
- C12N15/8227—Root-specific
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
- C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
- C12N15/823—Reproductive tissue-specific promoters
- C12N15/8231—Male-specific, e.g. anther, tapetum, pollen
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
- C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
- C12N15/823—Reproductive tissue-specific promoters
- C12N15/8234—Seed-specific, e.g. embryo, endosperm
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants
Abstract
La presente proporciona moléculas de ADN y constructos, que incluyen sus secuencias de nucleótidos, de utilidad para modular la expresión génica en plantas y células de plantas. La presente también proporciona plantas transgénicas, células de plantas, partes de plantas, semillas y productos de consumo que comprenden las moléculas de ADN operativamente ligadas a polinucleótidos transcribibles heterólogos, junto con métodos para su uso.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201161467875P | 2011-03-25 | 2011-03-25 |
Publications (1)
Publication Number | Publication Date |
---|---|
AR119721A2 true AR119721A2 (es) | 2022-01-05 |
Family
ID=45888508
Family Applications (5)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ARP120101006A AR085571A1 (es) | 2011-03-25 | 2012-03-26 | Elementos de regulacion de plantas y sus usos |
ARP190103028A AR116448A2 (es) | 2011-03-25 | 2019-10-23 | Elementos de regulación de plantas y sus usos |
ARP190103026A AR116446A2 (es) | 2011-03-25 | 2019-10-23 | Elementos de regulación de plantas y sus usos |
ARP190103025A AR116445A2 (es) | 2011-03-25 | 2019-10-23 | Elementos de regulación de plantas y sus usos |
ARP200101167A AR119721A2 (es) | 2011-03-25 | 2020-04-24 | Elementos de regulación de plantas y sus usos |
Family Applications Before (4)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ARP120101006A AR085571A1 (es) | 2011-03-25 | 2012-03-26 | Elementos de regulacion de plantas y sus usos |
ARP190103028A AR116448A2 (es) | 2011-03-25 | 2019-10-23 | Elementos de regulación de plantas y sus usos |
ARP190103026A AR116446A2 (es) | 2011-03-25 | 2019-10-23 | Elementos de regulación de plantas y sus usos |
ARP190103025A AR116445A2 (es) | 2011-03-25 | 2019-10-23 | Elementos de regulación de plantas y sus usos |
Country Status (24)
Country | Link |
---|---|
US (9) | US9062316B2 (es) |
EP (3) | EP2688906B1 (es) |
JP (6) | JP2014509866A (es) |
KR (5) | KR102003176B1 (es) |
CN (1) | CN103534267B (es) |
AP (1) | AP2013007141A0 (es) |
AR (5) | AR085571A1 (es) |
AU (5) | AU2012237662B2 (es) |
BR (1) | BR112013024144A2 (es) |
CA (5) | CA3004039C (es) |
CL (7) | CL2013002707A1 (es) |
CO (1) | CO6801642A2 (es) |
ES (3) | ES2666149T3 (es) |
HU (1) | HUE035893T2 (es) |
MX (2) | MX344605B (es) |
PH (4) | PH12017500576A1 (es) |
PL (2) | PL2733151T3 (es) |
PT (2) | PT2733151T (es) |
RS (1) | RS56086B1 (es) |
RU (1) | RU2644205C2 (es) |
UA (1) | UA117555C2 (es) |
UY (5) | UY39164A (es) |
WO (1) | WO2012134921A2 (es) |
ZA (1) | ZA201306899B (es) |
Families Citing this family (22)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR102003176B1 (ko) | 2011-03-25 | 2019-07-24 | 몬산토 테크놀로지 엘엘씨 | 식물 조절 요소 및 그의 용도 |
EA031636B1 (ru) * | 2011-05-13 | 2019-01-31 | Монсанто Текнолоджи Ллс | Регуляторные элементы растений и их применение |
AU2013362921B2 (en) | 2012-12-19 | 2018-03-01 | Monsanto Technology Llc | Plant regulatory elements and uses thereof |
BR102014021330A2 (pt) * | 2013-08-30 | 2015-09-22 | Dow Agrosciences Llc | construtos para expressão de transgenes usando elementos reguladores de genes de ubiquitina de panicum |
TW201527314A (zh) * | 2013-12-31 | 2015-07-16 | Dow Agrosciences Llc | 新穎玉米泛素啓動子(三) |
TW201527316A (zh) * | 2013-12-31 | 2015-07-16 | Dow Agrosciences Llc | 新穎玉米泛素啓動子(五) |
TW201527312A (zh) * | 2013-12-31 | 2015-07-16 | Dow Agrosciences Llc | 新穎玉米泛素啓動子(一) |
TW201527313A (zh) * | 2013-12-31 | 2015-07-16 | Dow Agrosciences Llc | 新穎玉米泛素啓動子(二) |
PE20161403A1 (es) | 2014-03-20 | 2016-12-29 | Monsanto Technology Llc | Evento de maiz transgenico mon 87419 y metodos para su uso |
SG11201807333UA (en) | 2016-03-11 | 2018-09-27 | Monsanto Technology Llc | Plant regulatory elements and uses thereof |
BR112018072417B1 (pt) | 2016-05-04 | 2023-03-14 | E. I. Du Pont De Nemours And Company | Polipeptídeo inseticida recombinante, polipeptídeo quimérico, composição, polinucleotídeo recombinante, construtos de dna, métodos para obter uma planta transgênica, métodos para inibir o crescimento ou extermínio de uma praga de inseto ou população de praga, método para obter uma célula procariótica transformada, célula procariótica transformada e método para modificar geneticamente o polipeptídeo inseticida |
CA3024452A1 (en) * | 2016-05-24 | 2017-11-30 | Monsanto Technology Llc | Plant regulatory elements and uses thereof |
TW201805425A (zh) * | 2016-06-16 | 2018-02-16 | 艾格里遺傳學股份有限公司 | 用於轉殖基因表現之植物啟動子與3’utr |
CA2962852A1 (en) | 2017-03-31 | 2018-09-30 | Chuanxin Sun | Carbohydrate producing plant material |
EA202091620A1 (ru) | 2018-02-02 | 2020-10-01 | Монсанто Текнолоджи Ллс | Объект кукурузы mon87429 и способы его использования |
JP7164333B2 (ja) | 2018-06-27 | 2022-11-01 | 株式会社日立製作所 | 個人情報分析システム |
US11414669B2 (en) | 2018-09-06 | 2022-08-16 | Monsanto Technology Llc | Compositions and methods for genome editing in planta |
CN109486818B (zh) * | 2018-11-14 | 2021-06-01 | 中国热带农业科学院三亚研究院 | 狗尾草u6启动子基因及应用 |
EP4143214A1 (en) * | 2020-04-28 | 2023-03-08 | Monsanto Technology LLC | Plant regulatory elements and uses thereof |
CN111518807A (zh) * | 2020-04-30 | 2020-08-11 | 深圳大学 | 抑制薇甘菊光捕获蛋白表达的寡核苷酸链、重组载体及构建方法 |
US20220162625A1 (en) * | 2020-11-11 | 2022-05-26 | Monsanto Technology Llc | Methods to improve site-directed integration frequency |
JP7185809B1 (ja) | 2021-03-30 | 2022-12-07 | 日本碍子株式会社 | スパッタリングターゲット |
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- 2020-04-24 AR ARP200101167A patent/AR119721A2/es unknown
- 2020-06-12 US US16/900,717 patent/US11466282B2/en active Active
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2022
- 2022-08-19 US US17/821,123 patent/US20230145023A1/en active Pending
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