AR092479A1 - Plataforma de integracion de transgenes modificados por ingenieria genetica (etip) para reconocimiento de genes y apilamiento de rasgos - Google Patents

Plataforma de integracion de transgenes modificados por ingenieria genetica (etip) para reconocimiento de genes y apilamiento de rasgos

Info

Publication number
AR092479A1
AR092479A1 ARP130103190A ARP130103190A AR092479A1 AR 092479 A1 AR092479 A1 AR 092479A1 AR P130103190 A ARP130103190 A AR P130103190A AR P130103190 A ARP130103190 A AR P130103190A AR 092479 A1 AR092479 A1 AR 092479A1
Authority
AR
Argentina
Prior art keywords
etip
genetic engineering
plant
gene
stacking
Prior art date
Application number
ARP130103190A
Other languages
English (en)
Inventor
R Webb Steven
Gupta Manju
Mike Ainley W
J Henry Matthew
Mason John
Kumar Sandeep
Novak Stephen
Hayden Matthew
Cogan Noel
Sawbridge Tim
Spangenberg German
Forster John
Original Assignee
Dow Agrosciences Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dow Agrosciences Llc filed Critical Dow Agrosciences Llc
Publication of AR092479A1 publication Critical patent/AR092479A1/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Botany (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Physiology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Dispersion Chemistry (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Se describe una Plataforma de Integración de Transgenes Modificados por Ingeniería Genética (ETIP) que puede insertarse en forma aleatoria o en lugares determinados en genomas de plantas para facilitar la rápida selección y detección de un GOI (gen de interés) que es perfectamente reconocido (tanto los extremos 5 como 3) en la ubicación genómica de ETIP. Un elemento en la presente descripción es la introducción de roturas de doble cadena específicas dentro de la ETIP. En algunas realizaciones, una ETIP se describe utilizando sitios de unión de nucleasa de dedo de zinc, pero puede utilizar otras tecnologías de reconocimiento tal como meganucleasas, CRISPRs, TALs, o cierres de leucina. También se describen composiciones de plantas transgénicas y métodos para producir las mismas en donde el ADN donante o de carga expresa uno o más productos de una secuencia de ácido nucleico exógeno (por ejemplo, proteína o ARN) que ha sido establemente integrado en una ETIP en una célula de planta. En realizaciones, la ETIP facilita la prueba de candidatos de genes y vectores de expresión de plantas a partir de ideación por fases de desarrollo.
ARP130103190A 2012-09-07 2013-09-06 Plataforma de integracion de transgenes modificados por ingenieria genetica (etip) para reconocimiento de genes y apilamiento de rasgos AR092479A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201261697882P 2012-09-07 2012-09-07

Publications (1)

Publication Number Publication Date
AR092479A1 true AR092479A1 (es) 2015-04-22

Family

ID=50237646

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP130103190A AR092479A1 (es) 2012-09-07 2013-09-06 Plataforma de integracion de transgenes modificados por ingenieria genetica (etip) para reconocimiento de genes y apilamiento de rasgos

Country Status (18)

Country Link
US (1) US10640779B2 (es)
EP (1) EP2893025B1 (es)
JP (1) JP6505599B2 (es)
KR (1) KR102243727B1 (es)
CN (2) CN104838001B (es)
AR (1) AR092479A1 (es)
AU (1) AU2013312352B2 (es)
BR (1) BR112015004848B8 (es)
CA (1) CA2883792C (es)
CL (1) CL2015000565A1 (es)
HK (1) HK1211054A1 (es)
IL (1) IL237534B (es)
NZ (1) NZ705675A (es)
RU (1) RU2666916C2 (es)
TW (1) TWI669396B (es)
UA (1) UA119135C2 (es)
WO (1) WO2014039872A1 (es)
ZA (1) ZA201501735B (es)

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