ZA200103103B - High temperature reverse transcription using mutant DNA polymerases. - Google Patents

High temperature reverse transcription using mutant DNA polymerases.

Info

Publication number
ZA200103103B
ZA200103103B ZA200103103A ZA200103103A ZA200103103B ZA 200103103 B ZA200103103 B ZA 200103103B ZA 200103103 A ZA200103103 A ZA 200103103A ZA 200103103 A ZA200103103 A ZA 200103103A ZA 200103103 B ZA200103103 B ZA 200103103B
Authority
ZA
South Africa
Prior art keywords
dna polymerases
reverse transcription
high temperature
mutant dna
temperature reverse
Prior art date
Application number
ZA200103103A
Other languages
English (en)
Inventor
Edward Soh Smith
Carita Maria Elfstrom
David Harrow Gelfand
Russell Gene Higuchi
Thomas William Myers
Nancy Jeneane Shoenbrunner
Alice Ming Wang
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Publication of ZA200103103B publication Critical patent/ZA200103103B/xx

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/10—Nucleotidyl transfering
    • C12Q2521/101—DNA polymerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/10—Nucleotidyl transfering
    • C12Q2521/107—RNA dependent DNA polymerase,(i.e. reverse transcriptase)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/125—Specific component of sample, medium or buffer

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
ZA200103103A 2000-04-18 2001-04-17 High temperature reverse transcription using mutant DNA polymerases. ZA200103103B (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US19833600P 2000-04-18 2000-04-18

Publications (1)

Publication Number Publication Date
ZA200103103B true ZA200103103B (en) 2002-10-17

Family

ID=22732954

Family Applications (1)

Application Number Title Priority Date Filing Date
ZA200103103A ZA200103103B (en) 2000-04-18 2001-04-17 High temperature reverse transcription using mutant DNA polymerases.

Country Status (16)

Country Link
US (1) US7179590B2 (fr)
EP (1) EP1152062B1 (fr)
JP (2) JP3844975B2 (fr)
KR (1) KR100798671B1 (fr)
CN (1) CN1273611C (fr)
AT (1) ATE339518T1 (fr)
AU (1) AU781191B2 (fr)
BR (1) BR0101493B1 (fr)
CA (1) CA2342277C (fr)
DE (1) DE60122962T2 (fr)
DK (1) DK1152062T3 (fr)
ES (1) ES2270915T3 (fr)
HK (1) HK1044569B (fr)
MX (1) MXPA01003759A (fr)
TR (1) TR200101093A2 (fr)
ZA (1) ZA200103103B (fr)

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US7745125B2 (en) 2004-06-28 2010-06-29 Roche Molecular Systems, Inc. 2′-terminator related pyrophosphorolysis activated polymerization
US20070128620A1 (en) 2005-07-15 2007-06-07 Applera Corporation Hot start reverse transcription by primer design
US8962293B2 (en) 2006-10-18 2015-02-24 Roche Molecular Systems, Inc. DNA polymerases and related methods
JP5191041B2 (ja) * 2007-04-05 2013-04-24 エフ．ホフマン−ラ ロシュ アーゲー 急速ワンステップｒｔ−ｐｃｒ
US8026091B2 (en) * 2007-07-13 2011-09-27 Roche Molecular Systems, Inc. DNA polymerases and related methods
US10150990B2 (en) 2008-04-21 2018-12-11 Roche Molecular Systems, Inc. Ribonucleotide tag nucleic acid detection
JP5641939B2 (ja) * 2008-10-28 2014-12-17 学校法人関西学院 変異型逆転写ｄｎａポリメラーゼ
FR2940125B1 (fr) 2008-12-23 2013-03-22 Isp Investments Inc Composition cosmetique ou pharmaceutique apaisante comprenant un peptide activateur de la hmg-coa reductase
FR2940291B1 (fr) * 2008-12-23 2012-12-21 Isp Investments Inc Peptide derive d'hmg-coa reductase et composition cosmetique ou pharmaceutique le contenant
FR2944445B1 (fr) 2009-04-15 2013-08-16 Isp Investments Inc Composition cosmetique et/ou pharmaceutique comprenant un hydrolysat peptidique apaisant
FR2944526B1 (fr) 2009-04-15 2013-05-10 Isp Investments Inc Composition cosmetique et/ou pharmaceutique comprenant un hydrolysat peptidique capable de renforcer la fonction barriere
US8933036B2 (en) 2009-04-15 2015-01-13 Isp Investments Inc. Cosmetic and/or pharmaceutical composition comprising a yeast peptide hydrolysate and use of the yeast peptide hydrolysate as an active agent for strengthening hair
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CN103025868B (zh) 2010-06-18 2015-07-01 霍夫曼-拉罗奇有限公司 具有增强的3’-错配辨别力的dna聚合酶
US8759062B2 (en) 2010-06-18 2014-06-24 Roche Molecular Systems, Inc. DNA polymerases with increased 3′- mismatch discrimination
EP2675897B1 (fr) 2011-02-15 2016-05-11 Roche Diagnostics GmbH Adn polymérases avec discrimination accrue des mésappariements à l'extrémité 3'
ES2561885T3 (es) 2011-04-11 2016-03-01 F. Hoffmann-La Roche Ag ADN polimerasas de actividad mejorada
JP5977000B2 (ja) * 2011-07-12 2016-08-24 アークレイ株式会社 核酸の増幅検出方法およびキット
ES2553400T3 (es) * 2011-07-28 2015-12-09 F. Hoffmann-La Roche Ag ADN polimerasas con actividad mejorada
CA2858264C (fr) 2011-12-08 2018-01-02 F. Hoffmann-La Roche Ag Adn polymerases presentant une activite amelioree
WO2013083262A1 (fr) 2011-12-08 2013-06-13 Roche Diagnostics Gmbh Adn polymérases avec activité améliorée
JP6140182B2 (ja) 2011-12-08 2017-05-31 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 改善された活性を有するｄｎａポリメラーゼ
WO2013101783A2 (fr) 2011-12-30 2013-07-04 Bio-Rad Laboratories, Inc. Procédés et compositions pour la mise en œuvre de réactions d'amplification d'acide nucléique
ES2713653T3 (es) 2013-03-15 2019-05-23 Ibis Biosciences Inc Análogos nucleotídicos para secuenciación
US20150050697A1 (en) 2013-08-19 2015-02-19 Abbott Molecular Inc. Nucleotide analogs
US9868944B2 (en) * 2014-12-19 2018-01-16 Roche Molecular Systems, Inc. Reaction mixtures
WO2016183294A1 (fr) * 2015-05-12 2016-11-17 Dna Polymerase Technology, Inc. Polymérases mutantes et leurs utilisations
CN107541507A (zh) * 2016-06-29 2018-01-05 厦门大学 一种rna反转录扩增方法
WO2018136404A1 (fr) 2017-01-19 2018-07-26 Asuragen, Inc. Méthodes d'amplification d'arn
JP7363063B2 (ja) * 2018-03-15 2023-10-18 東洋紡株式会社 変異型ｄｎａポリメラーゼ
US20210032609A1 (en) 2018-03-21 2021-02-04 Roche Molecular Systems, Inc. Dna polymerases for efficient and effective incorporation of methylated-dntps
WO2019212023A1 (fr) * 2018-05-02 2019-11-07 タカラバイオ株式会社 Procédé de traitement de virus à arn
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Also Published As

Publication number Publication date
KR20010098620A (ko) 2001-11-08
JP4468919B2 (ja) 2010-05-26
KR100798671B1 (ko) 2008-01-28
BR0101493A (pt) 2001-11-13
US20020012970A1 (en) 2002-01-31
CN1273611C (zh) 2006-09-06
JP2006304804A (ja) 2006-11-09
EP1152062B1 (fr) 2006-09-13
AU781191B2 (en) 2005-05-12
TR200101093A2 (tr) 2002-02-21
MXPA01003759A (es) 2004-09-10
DE60122962D1 (de) 2006-10-26
BR0101493B1 (pt) 2013-07-16
JP2002253234A (ja) 2002-09-10
AU3516501A (en) 2001-10-25
ATE339518T1 (de) 2006-10-15
EP1152062A2 (fr) 2001-11-07
CN1344802A (zh) 2002-04-17
CA2342277C (fr) 2008-01-15
JP3844975B2 (ja) 2006-11-15
ES2270915T3 (es) 2007-04-16
DE60122962T2 (de) 2007-04-26
EP1152062A3 (fr) 2002-09-11
HK1044569A1 (en) 2002-10-25
DK1152062T3 (da) 2007-01-22
HK1044569B (zh) 2007-04-20
US7179590B2 (en) 2007-02-20
CA2342277A1 (fr) 2001-10-18

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