WO2006030455A8 - Polymerase d'adn presentant une activite de deplacement de brins - Google Patents

Polymerase d'adn presentant une activite de deplacement de brins

Info

Publication number
WO2006030455A8
WO2006030455A8 PCT/IS2005/000022 IS2005000022W WO2006030455A8 WO 2006030455 A8 WO2006030455 A8 WO 2006030455A8 IS 2005000022 W IS2005000022 W IS 2005000022W WO 2006030455 A8 WO2006030455 A8 WO 2006030455A8
Authority
WO
WIPO (PCT)
Prior art keywords
polymerases
strand displacement
dna polymerases
displacement activity
species
Prior art date
Application number
PCT/IS2005/000022
Other languages
English (en)
Other versions
WO2006030455A1 (fr
Inventor
Sigridur Hjorleifsdottir
Sveinn Ernstson
Thorarinn Blondal
Arnthor Aevarsson
Gudmundur Oli Hreggvidsson
Jakob Kristjansson
Original Assignee
Prokaria Ehf
Sigridur Hjorleifsdottir
Sveinn Ernstson
Thorarinn Blondal
Arnthor Aevarsson
Gudmundur Oli Hreggvidsson
Jakob Kristjansson
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Prokaria Ehf, Sigridur Hjorleifsdottir, Sveinn Ernstson, Thorarinn Blondal, Arnthor Aevarsson, Gudmundur Oli Hreggvidsson, Jakob Kristjansson filed Critical Prokaria Ehf
Priority to US11/662,879 priority Critical patent/US20080311626A1/en
Publication of WO2006030455A1 publication Critical patent/WO2006030455A1/fr
Publication of WO2006030455A8 publication Critical patent/WO2006030455A8/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

L'invention porte sur de nouveaux polymérases d'ADN à déplacement de brins pouvant être utilisés dans des réactions d'amplification de déplacement de brins rapides et efficaces. Les polymérases sont nettement plus thermostables que les polymérases de la technique antérieure et présentent une activité élevée à des températures élevées. L'invention porte aussi sur des gènes codant les polymérases et sur des vecteurs contenant ces gènes. Des polymérases représentatifs de l'invention peuvent être obtenus à partir de souches bactériennes des espèces Thermus antranikianii et Thermus brockianus mais aussi à partir d'échantillons prélevés dans l'environnement par isolation des espèces sources.
PCT/IS2005/000022 2004-09-17 2005-09-19 Polymerase d'adn presentant une activite de deplacement de brins WO2006030455A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US11/662,879 US20080311626A1 (en) 2004-09-17 2005-09-19 Dna Polymerases Having Strand Displacement Activity

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US61056204P 2004-09-17 2004-09-17
IS746104 2004-09-17
IS7461 2004-09-17
US60/610,562 2004-09-17

Publications (2)

Publication Number Publication Date
WO2006030455A1 WO2006030455A1 (fr) 2006-03-23
WO2006030455A8 true WO2006030455A8 (fr) 2007-05-03

Family

ID=37969691

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IS2005/000022 WO2006030455A1 (fr) 2004-09-17 2005-09-19 Polymerase d'adn presentant une activite de deplacement de brins

Country Status (1)

Country Link
WO (1) WO2006030455A1 (fr)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1914303A1 (fr) * 2006-10-09 2008-04-23 Qiagen GmbH ADN polymerases de Thermus eggertssonii
GB0803628D0 (en) 2008-02-28 2008-04-02 Genesys Ltd Enzyme
JP2010161935A (ja) * 2009-01-13 2010-07-29 Fujifilm Corp 核酸増幅反応におけるばらつきを低減する方法
US9896671B2 (en) 2013-04-05 2018-02-20 Bioron Gmbh DNA polymerases
PT3152331T (pt) 2014-06-06 2019-11-05 Univ Cornell Método para identificação e enumeração de alterações de sequência de ácidos nucleicos, expressão, cópia ou metilação de adn, utilizando reações de nuclease, ligase, polimerase e sequenciação combinadas
GB201721053D0 (en) 2017-12-15 2018-01-31 Univ I Tromsø - Norges Arktiske Univ DNA polymerases
CN113454238A (zh) 2019-01-25 2021-09-28 哈佛大学校长及研究员协会 用于合成核酸的组合物和方法
CN114761548B (zh) * 2019-10-01 2025-01-07 特罗姆瑟大学-挪威北极圈大学 海洋dna聚合酶i
CA3161183A1 (fr) 2019-12-12 2021-06-17 President And Fellows Of Harvard College Compositions et methodes pour le codage a barres biomoleculaire dirige par la lumiere
WO2022082200A2 (fr) * 2020-10-16 2022-04-21 Geaenzymes Co. Saturation biochimique de molécules et son utilisation
US12031179B2 (en) * 2020-10-30 2024-07-09 Singular Genomics Systems, Inc. Methods and compositions for reducing nucleotide impurities

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992009689A1 (fr) * 1990-12-03 1992-06-11 Stratagene Adn polymerase i thermostable purifiee de $i(pyrococcus furiosus)
US5648211A (en) * 1994-04-18 1997-07-15 Becton, Dickinson And Company Strand displacement amplification using thermophilic enzymes
US7049101B1 (en) * 1997-08-06 2006-05-23 Diversa Corporation Enzymes having high temperature polymerase activity and methods of use thereof
AU2002346498A1 (en) * 2001-11-30 2003-06-17 Applera Corporation Thermus brockianus nucleic acid polymerases

Also Published As

Publication number Publication date
WO2006030455A1 (fr) 2006-03-23

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