ZA200103103B - High temperature reverse transcription using mutant DNA polymerases. - Google Patents

High temperature reverse transcription using mutant DNA polymerases.

Info

Publication number
ZA200103103B
ZA200103103B ZA200103103A ZA200103103A ZA200103103B ZA 200103103 B ZA200103103 B ZA 200103103B ZA 200103103 A ZA200103103 A ZA 200103103A ZA 200103103 A ZA200103103 A ZA 200103103A ZA 200103103 B ZA200103103 B ZA 200103103B
Authority
ZA
South Africa
Prior art keywords
dna polymerases
reverse transcription
high temperature
mutant dna
temperature reverse
Prior art date
Application number
ZA200103103A
Other languages
English (en)
Inventor
Edward Soh Smith
Carita Maria Elfstrom
David Harrow Gelfand
Russell Gene Higuchi
Thomas William Myers
Nancy Jeneane Shoenbrunner
Alice Ming Wang
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Publication of ZA200103103B publication Critical patent/ZA200103103B/xx

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/12Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241Nucleotidyltransferases (2.7.7)
    • C12N9/1252DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/12Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241Nucleotidyltransferases (2.7.7)
    • C12N9/1276RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/686Polymerase chain reaction [PCR]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00Reaction characterised by the enzymatic activity
    • C12Q2521/10Nucleotidyl transfering
    • C12Q2521/101DNA polymerase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00Reaction characterised by the enzymatic activity
    • C12Q2521/10Nucleotidyl transfering
    • C12Q2521/107RNA dependent DNA polymerase,(i.e. reverse transcriptase)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00Reactions demanding special reaction conditions
    • C12Q2527/125Specific component of sample, medium or buffer

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
ZA200103103A 2000-04-18 2001-04-17 High temperature reverse transcription using mutant DNA polymerases. ZA200103103B (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US19833600P 2000-04-18 2000-04-18

Publications (1)

Publication Number Publication Date
ZA200103103B true ZA200103103B (en) 2002-10-17

Family

ID=22732954

Family Applications (1)

Application Number Title Priority Date Filing Date
ZA200103103A ZA200103103B (en) 2000-04-18 2001-04-17 High temperature reverse transcription using mutant DNA polymerases.

Country Status (16)

Country Link
US (1) US7179590B2 (xx)
EP (1) EP1152062B1 (xx)
JP (2) JP3844975B2 (xx)
KR (1) KR100798671B1 (xx)
CN (1) CN1273611C (xx)
AT (1) ATE339518T1 (xx)
AU (1) AU781191B2 (xx)
BR (1) BR0101493B1 (xx)
CA (1) CA2342277C (xx)
DE (1) DE60122962T2 (xx)
DK (1) DK1152062T3 (xx)
ES (1) ES2270915T3 (xx)
HK (1) HK1044569B (xx)
MX (1) MXPA01003759A (xx)
TR (1) TR200101093A2 (xx)
ZA (1) ZA200103103B (xx)

Families Citing this family (49)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040009486A1 (en) * 1999-10-29 2004-01-15 Sorge Joseph A. Compositions and methods utilizing DNA polymerases
US20050123940A1 (en) * 1999-10-29 2005-06-09 Stratagene California Compositions and methods for synthesizing cDNA
US20040265870A1 (en) * 2003-04-09 2004-12-30 Invitrogen Corporation Methods of synthesizing and labeling nucleic acid molecules
US7947817B2 (en) 2003-06-30 2011-05-24 Roche Molecular Systems, Inc. Synthesis and compositions of 2'-terminator nucleotides
US7417133B2 (en) 2004-02-27 2008-08-26 Institut Pasteur Methods for obtaining thermostable enzymes, DNA polymerase I variants from Thermus aquaticus having new catalytic activities, methods for obtaining the same, and applications of the same
US7745125B2 (en) 2004-06-28 2010-06-29 Roche Molecular Systems, Inc. 2′-terminator related pyrophosphorolysis activated polymerization
WO2007011901A2 (en) 2005-07-15 2007-01-25 Applera Corporation Hot start reverse transcription by primer design
US8962293B2 (en) 2006-10-18 2015-02-24 Roche Molecular Systems, Inc. DNA polymerases and related methods
JP5191041B2 (ja) * 2007-04-05 2013-04-24 エフ.ホフマン−ラ ロシュ アーゲー 急速ワンステップrt−pcr
US8026091B2 (en) * 2007-07-13 2011-09-27 Roche Molecular Systems, Inc. DNA polymerases and related methods
US10150990B2 (en) 2008-04-21 2018-12-11 Roche Molecular Systems, Inc. Ribonucleotide tag nucleic acid detection
WO2010050418A1 (ja) * 2008-10-28 2010-05-06 学校法人関西学院 変異型逆転写dnaポリメラーゼ
FR2940125B1 (fr) 2008-12-23 2013-03-22 Isp Investments Inc Composition cosmetique ou pharmaceutique apaisante comprenant un peptide activateur de la hmg-coa reductase
FR2940291B1 (fr) 2008-12-23 2012-12-21 Isp Investments Inc Peptide derive d'hmg-coa reductase et composition cosmetique ou pharmaceutique le contenant
FR2944526B1 (fr) 2009-04-15 2013-05-10 Isp Investments Inc Composition cosmetique et/ou pharmaceutique comprenant un hydrolysat peptidique capable de renforcer la fonction barriere
FR2944445B1 (fr) 2009-04-15 2013-08-16 Isp Investments Inc Composition cosmetique et/ou pharmaceutique comprenant un hydrolysat peptidique apaisant
US8933036B2 (en) 2009-04-15 2015-01-13 Isp Investments Inc. Cosmetic and/or pharmaceutical composition comprising a yeast peptide hydrolysate and use of the yeast peptide hydrolysate as an active agent for strengthening hair
CA2802000C (en) 2010-06-18 2016-08-16 F. Hoffmann-La Roche Ag Dna polymerases with increased 3'-mismatch discrimination
JP5876479B2 (ja) 2010-06-18 2016-03-02 エフ.ホフマン−ラ ロシュ アーゲーF. Hoffmann−La Roche Aktiengesellschaft 増大した3’末端ミスマッチ識別を有するdnaポリメラーゼ
US8735120B2 (en) 2010-06-18 2014-05-27 Roche Molecular Systems, Inc. DNA polymerases with increased 3′-mismatch discrimination
WO2011157438A1 (en) 2010-06-18 2011-12-22 Roche Diagnostics Gmbh Dna polymerases with increasesed 3'-mismatch discrimination
CA2802304C (en) 2010-06-18 2016-01-12 F.Hoffman-La Roche Ag Dna polymerases with increased 3'-mismatch discrimination
US8722378B2 (en) 2010-06-18 2014-05-13 Roche Molecular Systems, Inc. DNA polymerases with increased 3′-mismatch discrimination
WO2011157435A1 (en) 2010-06-18 2011-12-22 Roche Diagnostics Gmbh Dna polymerases with increased 3'-mismatch discrimination
WO2011157430A1 (en) 2010-06-18 2011-12-22 Roche Diagnostics Gmbh Dna polymerases with increased 3'-mismatch discrimination
JP5847201B2 (ja) 2011-02-15 2016-01-20 エフ.ホフマン−ラ ロシュ アーゲーF. Hoffmann−La Roche Aktiengesellschaft 増大した3’末端ミスマッチ識別能を有するdnaポリメラーゼ
WO2012139748A1 (en) 2011-04-11 2012-10-18 Roche Diagnostics Gmbh Dna polymerases with improved activity
JP5977000B2 (ja) * 2011-07-12 2016-08-24 アークレイ株式会社 核酸の増幅検出方法およびキット
US9090883B2 (en) 2011-07-28 2015-07-28 Roche Molecular Systems, Inc. DNA polymerases with improved activity
EP2788480B1 (en) 2011-12-08 2019-01-16 Roche Diagnostics GmbH Dna polymerases with improved activity
ES2569723T3 (es) 2011-12-08 2016-05-12 F. Hoffmann-La Roche Ag Polimerasas de DNA con actividad mejorada
WO2013083264A1 (en) 2011-12-08 2013-06-13 Roche Diagnostics Gmbh Dna polymerases with improved activity
WO2013101783A2 (en) 2011-12-30 2013-07-04 Bio-Rad Laboratories, Inc. Methods and compositions for performing nucleic acid amplification reactions
EP2970366B1 (en) 2013-03-15 2019-01-16 Ibis Biosciences, Inc. Nucleotide analogs for sequencing
ES2805004T3 (es) 2013-08-19 2021-02-10 Abbott Molecular Inc Análogos de nucleótidos
US9868944B2 (en) * 2014-12-19 2018-01-16 Roche Molecular Systems, Inc. Reaction mixtures
US11091745B2 (en) 2015-05-12 2021-08-17 Dna Polymerase Technology, Inc. Mutant polymerases and uses thereof
CN107541507A (zh) * 2016-06-29 2018-01-05 厦门大学 一种rna反转录扩增方法
WO2018136404A1 (en) 2017-01-19 2018-07-26 Asuragen, Inc. Methods of rna amplification
JP7363063B2 (ja) * 2018-03-15 2023-10-18 東洋紡株式会社 変異型dnaポリメラーゼ
JP7319990B2 (ja) 2018-03-21 2023-08-02 エフ. ホフマン-ラ ロシュ アーゲー メチル化dNTPを効率的かつ効果的に組み込むDNAポリメラーゼ
WO2019212023A1 (ja) * 2018-05-02 2019-11-07 タカラバイオ株式会社 Rnaウイルスの処理方法
EP3822349A4 (en) * 2018-07-13 2022-04-06 Takara Bio Inc. DNA POLYMERASE MUTATION SUITABLE FOR NUCLEIC ACID AMPLIFICATION FROM RNA
GB201812192D0 (en) 2018-07-26 2018-09-12 Ttp Plc Variable temperature reactor, heater and control circuit for the same
CN108949960A (zh) * 2018-08-13 2018-12-07 郑州安图生物工程股份有限公司 人cyp2c19基因多态性检测试剂盒
JP2022500046A (ja) 2018-09-13 2022-01-04 エフ.ホフマン−ラ ロシュ アーゲーF. Hoffmann−La Roche Aktiengesellschaft 改善されたストランド置換能力を有する変異体dnaポリメラーゼ
EP4074839A1 (en) 2021-04-16 2022-10-19 Biotype GmbH Optimized oligonucleotide tx probe for a multiplexing analysis of nucleic acids and a multiplexing method
EP4350002A1 (en) 2022-10-07 2024-04-10 Biotype GmbH Nachweis von molekularen analyten auf der grundlage massgeschneiderter sondenkonkurrenz
CN115975978B (zh) * 2023-02-01 2023-08-01 珠海宝锐生物科技有限公司 Bst DNA聚合酶大片段突变体及其应用

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5310652A (en) 1986-08-22 1994-05-10 Hoffman-La Roche Inc. Reverse transcription with thermostable DNA polymerase-high temperature reverse transcription
US5374553A (en) * 1986-08-22 1994-12-20 Hoffmann-La Roche Inc. DNA encoding a thermostable nucleic acid polymerase enzyme from thermotoga maritima
US5561058A (en) 1986-08-22 1996-10-01 Hoffmann-La Roche Inc. Methods for coupled high temperatures reverse transcription and polymerase chain reactions
JP2582980B2 (ja) * 1990-09-28 1997-02-19 エフ.ホフマン−ラ ロシュ アクチェンゲゼルシャフト モルモシポ・アフリカヌスからの純化された熱安定性核酸ポリメラーゼ
US6083686A (en) * 1990-10-26 2000-07-04 Johnson & Johnson Clinical Diagnostic Systems, Inc. Increased production of Thermus aquaticus DNA polymerase in E. coli
WO1995014770A1 (en) 1993-11-25 1995-06-01 Pacific Enzymes (1993) Limited Improved polymerase
US6015668A (en) * 1994-09-30 2000-01-18 Life Technologies, Inc. Cloned DNA polymerases from thermotoga and mutants thereof
ATE143057T1 (de) 1994-10-17 1996-10-15 Harvard College Dns polymerase mit veränderter nukleotid- bindungstelle
US5614365A (en) * 1994-10-17 1997-03-25 President & Fellow Of Harvard College DNA polymerase having modified nucleotide binding site for DNA sequencing
US5830714A (en) * 1996-04-17 1998-11-03 Molecular Biology Resources, Inc. Biologically active fragment of bacillus stearothermophilus DNA polymerase
CZ293215B6 (cs) 1996-08-06 2004-03-17 F. Hoffmann-La Roche Ag Rekombinantní tepelně stálá DNA polymeráza, způsob její přípravy a prostředek, který ji obsahuje
WO1998040496A1 (en) 1997-03-12 1998-09-17 The Perkin-Elmer Corporation Dna polymerases having improved labeled nucleotide incorporation properties
CA2243985C (en) 1997-09-11 2004-08-10 F. Hoffmann-La Roche Ag Thermostable dna polymerases incorporating nucleoside triphosphates labeled with fluorescein family dyes
WO2000071739A1 (en) 1999-05-22 2000-11-30 Epicentre Technologies Corporation Reverse transcription activity from bacillus stearothermophilus dna polymerase in the presence of magnesium

Also Published As

Publication number Publication date
CA2342277C (en) 2008-01-15
US7179590B2 (en) 2007-02-20
ATE339518T1 (de) 2006-10-15
HK1044569B (zh) 2007-04-20
CN1273611C (zh) 2006-09-06
JP4468919B2 (ja) 2010-05-26
JP2002253234A (ja) 2002-09-10
TR200101093A2 (tr) 2002-02-21
EP1152062A2 (en) 2001-11-07
EP1152062B1 (en) 2006-09-13
MXPA01003759A (es) 2004-09-10
CN1344802A (zh) 2002-04-17
HK1044569A1 (en) 2002-10-25
AU3516501A (en) 2001-10-25
EP1152062A3 (en) 2002-09-11
BR0101493A (pt) 2001-11-13
AU781191B2 (en) 2005-05-12
KR100798671B1 (ko) 2008-01-28
CA2342277A1 (en) 2001-10-18
JP2006304804A (ja) 2006-11-09
DK1152062T3 (da) 2007-01-22
DE60122962D1 (de) 2006-10-26
BR0101493B1 (pt) 2013-07-16
KR20010098620A (ko) 2001-11-08
US20020012970A1 (en) 2002-01-31
ES2270915T3 (es) 2007-04-16
JP3844975B2 (ja) 2006-11-15
DE60122962T2 (de) 2007-04-26

Similar Documents

Publication Publication Date Title
HK1044569A1 (en) High temperature reverse transcription using mutant dna polymerases
EP2272952B8 (en) Thermostable or thermoactive DNA polymerase with attenuated 3'-5' exonuclease activity
WO2002101022A3 (en) Method for rapid amplification of dna
EP1801213A3 (en) Polymerase
WO2004058942A3 (en) Dna polymerase blends and uses thereof
AU2002351193A8 (en) Parallel polymorphism scoring by amplification and error correction
DK1409667T3 (da) Fremgangsmåde til fremstilling af polynucleotidvarianter
WO2001023411A3 (en) Incorporation of modified nucleotides by archaeon dna polymerases and related methods
EA200100990A1 (ru) Способ амплификации последовательности нуклеиновой кислоты
HK1046021A1 (en) Methods and compositions for linear isothermal amplification of polynucleotide sequences
DE60112746D1 (de) Kälteempfindliche dna-polymerasemutanten
WO2001077390A3 (en) Process for allele discrimation utilizing primer extension
WO2006030455A8 (en) Dna polymerases having strand displacement activity
WO2005113760A3 (en) Thermostable y-family polymerases and chimeras
AU2002352902A1 (en) Thermus thermophilus nucleic acid polymerases
AU2002346498A1 (en) Thermus brockianus nucleic acid polymerases
WO2003048310A3 (en) Thermus oshimai nucleic acid polymerases
EP1466013A4 (en) NUCLEIC ACID POLYMERASES FROM THERMUS SCOTODUCTUS
DE60228089D1 (de) Nukleinsäureamplifikation mit duplexzwischenprodukten
AU2003266255A1 (en) Method for amplification of nucleic acids of low complexity
WO2006081463A3 (en) Thermococcus zilligii dna polymerases and variants thereof
AU2002215010A1 (en) Thermostable polymerase based on thermococcus pacificus
EP1598431A4 (en) PROCESS FOR DETECTING CHLAMYDIA TRACHOMATIS AND KIT THEREFOR
EP1548112A4 (en) METHOD FOR THE AMPLIFICATION OF NUCLEIC ACID, KIT FOR THE AMPLIFICATION OF NUCLEIC ACID, METHOD FOR THE DETECTION OF SINGLE-NUCLEOTIDE POLYMORPHISM AND REAGENT KIT FOR THE DETECTION OF SINGLE-NUCLEOTIDE POLYMORPHISM
WO2002103032A3 (en) Catalases, nucleic acids encoding them and methods for making and using them