ZA200103103B - High temperature reverse transcription using mutant DNA polymerases. - Google Patents

High temperature reverse transcription using mutant DNA polymerases.

Info

Publication number
ZA200103103B
ZA200103103B ZA200103103A ZA200103103A ZA200103103B ZA 200103103 B ZA200103103 B ZA 200103103B ZA 200103103 A ZA200103103 A ZA 200103103A ZA 200103103 A ZA200103103 A ZA 200103103A ZA 200103103 B ZA200103103 B ZA 200103103B
Authority
ZA
South Africa
Prior art keywords
dna polymerases
reverse transcription
high temperature
mutant dna
temperature reverse
Prior art date
Application number
ZA200103103A
Other languages
English (en)
Inventor
Edward Soh Smith
Carita Maria Elfstrom
David Harrow Gelfand
Russell Gene Higuchi
Thomas William Myers
Nancy Jeneane Shoenbrunner
Alice Ming Wang
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Publication of ZA200103103B publication Critical patent/ZA200103103B/xx

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/12Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241Nucleotidyltransferases (2.7.7)
    • C12N9/1252DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/12Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241Nucleotidyltransferases (2.7.7)
    • C12N9/1276RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/686Polymerase chain reaction [PCR]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00Reaction characterised by the enzymatic activity
    • C12Q2521/10Nucleotidyl transfering
    • C12Q2521/101DNA polymerase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00Reaction characterised by the enzymatic activity
    • C12Q2521/10Nucleotidyl transfering
    • C12Q2521/107RNA dependent DNA polymerase,(i.e. reverse transcriptase)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00Reactions demanding special reaction conditions
    • C12Q2527/125Specific component of sample, medium or buffer

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
ZA200103103A 2000-04-18 2001-04-17 High temperature reverse transcription using mutant DNA polymerases. ZA200103103B (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US19833600P 2000-04-18 2000-04-18

Publications (1)

Publication Number Publication Date
ZA200103103B true ZA200103103B (en) 2002-10-17

Family

ID=22732954

Family Applications (1)

Application Number Title Priority Date Filing Date
ZA200103103A ZA200103103B (en) 2000-04-18 2001-04-17 High temperature reverse transcription using mutant DNA polymerases.

Country Status (16)

Country Link
US (1) US7179590B2 (xx)
EP (1) EP1152062B1 (xx)
JP (2) JP3844975B2 (xx)
KR (1) KR100798671B1 (xx)
CN (1) CN1273611C (xx)
AT (1) ATE339518T1 (xx)
AU (1) AU781191B2 (xx)
BR (1) BR0101493B1 (xx)
CA (1) CA2342277C (xx)
DE (1) DE60122962T2 (xx)
DK (1) DK1152062T3 (xx)
ES (1) ES2270915T3 (xx)
HK (1) HK1044569B (xx)
MX (1) MXPA01003759A (xx)
TR (1) TR200101093A2 (xx)
ZA (1) ZA200103103B (xx)

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US8962293B2 (en) 2006-10-18 2015-02-24 Roche Molecular Systems, Inc. DNA polymerases and related methods
JP5191041B2 (ja) * 2007-04-05 2013-04-24 エフ.ホフマン−ラ ロシュ アーゲー 急速ワンステップrt−pcr
US8026091B2 (en) * 2007-07-13 2011-09-27 Roche Molecular Systems, Inc. DNA polymerases and related methods
US10150990B2 (en) 2008-04-21 2018-12-11 Roche Molecular Systems, Inc. Ribonucleotide tag nucleic acid detection
WO2010050418A1 (ja) * 2008-10-28 2010-05-06 学校法人関西学院 変異型逆転写dnaポリメラーゼ
FR2940125B1 (fr) 2008-12-23 2013-03-22 Isp Investments Inc Composition cosmetique ou pharmaceutique apaisante comprenant un peptide activateur de la hmg-coa reductase
FR2940291B1 (fr) 2008-12-23 2012-12-21 Isp Investments Inc Peptide derive d'hmg-coa reductase et composition cosmetique ou pharmaceutique le contenant
FR2944445B1 (fr) 2009-04-15 2013-08-16 Isp Investments Inc Composition cosmetique et/ou pharmaceutique comprenant un hydrolysat peptidique apaisant
FR2944526B1 (fr) 2009-04-15 2013-05-10 Isp Investments Inc Composition cosmetique et/ou pharmaceutique comprenant un hydrolysat peptidique capable de renforcer la fonction barriere
US8933036B2 (en) 2009-04-15 2015-01-13 Isp Investments Inc. Cosmetic and/or pharmaceutical composition comprising a yeast peptide hydrolysate and use of the yeast peptide hydrolysate as an active agent for strengthening hair
EP2582808B1 (en) 2010-06-18 2015-04-22 Roche Diagniostics GmbH Dna polymerases with increased 3'-mismatch discrimination
US8715991B2 (en) 2010-06-18 2014-05-06 Roche Molecular Systems, Inc. DNA polymerases with increased 3'-mismatch discrimination
EP2582804B1 (en) 2010-06-18 2015-02-25 Roche Diagnostics GmbH Dna polymerases with increased 3'-mismatch discrimination
WO2011157435A1 (en) 2010-06-18 2011-12-22 Roche Diagnostics Gmbh Dna polymerases with increased 3'-mismatch discrimination
AU2011267421B2 (en) 2010-06-18 2014-04-24 F. Hoffmann-La Roche Ag DNA polymerases with increased 3'-mismatch discrimination
US8722380B2 (en) 2010-06-18 2014-05-13 Roche Molecular Systems, Inc. DNA polymerases with increased 3′-mismatch discrimination
US8741617B2 (en) 2010-06-18 2014-06-03 Roche Molecular Systems, Inc. DNA polymerases with increased 3′-mismatch discrimination
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JP5847201B2 (ja) 2011-02-15 2016-01-20 エフ.ホフマン−ラ ロシュ アーゲーF. Hoffmann−La Roche Aktiengesellschaft 増大した3’末端ミスマッチ識別能を有するdnaポリメラーゼ
ES2561885T3 (es) 2011-04-11 2016-03-01 F. Hoffmann-La Roche Ag ADN polimerasas de actividad mejorada
JP5977000B2 (ja) * 2011-07-12 2016-08-24 アークレイ株式会社 核酸の増幅検出方法およびキット
CN103687943B (zh) 2011-07-28 2015-11-25 霍夫曼-拉罗奇有限公司 具有改进活性的dna聚合酶
ES2569723T3 (es) 2011-12-08 2016-05-12 F. Hoffmann-La Roche Ag Polimerasas de DNA con actividad mejorada
EP2788480B1 (en) 2011-12-08 2019-01-16 Roche Diagnostics GmbH Dna polymerases with improved activity
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US9868944B2 (en) * 2014-12-19 2018-01-16 Roche Molecular Systems, Inc. Reaction mixtures
US11091745B2 (en) 2015-05-12 2021-08-17 Dna Polymerase Technology, Inc. Mutant polymerases and uses thereof
CN107541507A (zh) * 2016-06-29 2018-01-05 厦门大学 一种rna反转录扩增方法
US11236384B2 (en) 2017-01-19 2022-02-01 Asuragen, Inc. Methods of RNA amplification
JP7363063B2 (ja) * 2018-03-15 2023-10-18 東洋紡株式会社 変異型dnaポリメラーゼ
WO2019180137A1 (en) 2018-03-21 2019-09-26 F. Hoffmann-La Roche Ag Dna polymerases for efficient and effective incorporation of methylated-dntps
JP7433217B2 (ja) * 2018-05-02 2024-02-19 タカラバイオ株式会社 Rnaウイルスの処理方法
WO2020013058A1 (ja) 2018-07-13 2020-01-16 タカラバイオ株式会社 Rnaからの核酸増幅反応に適したdnaポリメラーゼ変異体
GB201812192D0 (en) 2018-07-26 2018-09-12 Ttp Plc Variable temperature reactor, heater and control circuit for the same
CN108949960A (zh) * 2018-08-13 2018-12-07 郑州安图生物工程股份有限公司 人cyp2c19基因多态性检测试剂盒
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Also Published As

Publication number Publication date
CA2342277A1 (en) 2001-10-18
CA2342277C (en) 2008-01-15
MXPA01003759A (es) 2004-09-10
EP1152062B1 (en) 2006-09-13
JP2002253234A (ja) 2002-09-10
US20020012970A1 (en) 2002-01-31
HK1044569A1 (en) 2002-10-25
KR100798671B1 (ko) 2008-01-28
AU781191B2 (en) 2005-05-12
ATE339518T1 (de) 2006-10-15
DK1152062T3 (da) 2007-01-22
EP1152062A2 (en) 2001-11-07
DE60122962T2 (de) 2007-04-26
AU3516501A (en) 2001-10-25
HK1044569B (zh) 2007-04-20
BR0101493B1 (pt) 2013-07-16
BR0101493A (pt) 2001-11-13
JP3844975B2 (ja) 2006-11-15
CN1273611C (zh) 2006-09-06
CN1344802A (zh) 2002-04-17
ES2270915T3 (es) 2007-04-16
US7179590B2 (en) 2007-02-20
JP2006304804A (ja) 2006-11-09
KR20010098620A (ko) 2001-11-08
EP1152062A3 (en) 2002-09-11
DE60122962D1 (de) 2006-10-26
JP4468919B2 (ja) 2010-05-26
TR200101093A2 (tr) 2002-02-21

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