WO2019200921A1 - 一种基于二碳化三钛二维金属碳化物催化鲁米诺电化学发光探针的生物传感器及制备方法 - Google Patents
一种基于二碳化三钛二维金属碳化物催化鲁米诺电化学发光探针的生物传感器及制备方法 Download PDFInfo
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Abstract
Description
Claims (10)
- 一种基于二碳化三钛二维金属碳化物催化鲁米诺电化学发光探针,其特征是,包括纳米片Ti 3C 2MXenes、连接分子和生物识别分子1,所述纳米片Ti 3C 2MXenes与连接分子通过静电吸附连接,所述连接分子与生物识别分子1通过酰胺基团连接,所述连接分子含有伯胺基团或仲胺基团,且所述连接分子溶于水后能够带有正电荷,所述生物识别分子1为5’端带有羧基的单链DNA序列1,所述单链DNA序列1能够识别外泌体上的CD63蛋白质;优选的,所述连接分子为聚乙烯亚胺。
- 如权利要求1所述的探针,其特征是,所述单链DNA序列1由5’至3’的序列为TTTTTT CAC CCC CAC CTC GCT CCC GTG ACA CTA ATG CTA。
- 一种权利要求1或2所述的探针的制备方法,其特征是,将连接分子与纳米片Ti 3C 2MXenes置于水中混合均匀后,搅拌一段时间离心获得沉淀,将获得的沉淀与生物识别分子1进行酰胺反应即可获得;优选的,搅拌时间为1~1.5h;离心分离的转速超过10000rpm;优选的,所述酰胺反应的反应体系为1-(3-(二甲基氨基)丙基)-3-乙基碳化二亚胺盐酸盐和N-羟基琥珀酰亚胺钠盐。
- 一种与权利要求1或2所述的探针配合使用的生物传感器电极,其特征是,玻碳电极表面通过金纳米颗粒修饰,金纳米颗粒与至少含有两个氨基的分子中的一个氨基通过酰胺基团进行连接,至少含有两个氨基的分子中的另一个氨基与羧基封端的聚N-异丙基丙烯酰胺中的一个羧基通过酰胺基团使羧基封端的聚N-异丙基丙烯酰胺与至少含有两个氨基的分子进行连接,羧基封端的聚N-异丙基丙烯酰胺中的另一个羧基与生物识别分子2通过酰胺基团使羧基封端的聚N-异丙基丙烯酰胺与生物识别分子2进行连接,其中,生物识别分子2为5’端带有氨基的单链DNA序列2,所述单链DNA序列2能够识别外泌体上的EpCAM蛋白质;优选的,至少含有两个氨基的分子为乙二胺;优选的,所述羧基封端的聚N-异丙基丙烯酰胺的数均分子量为1000~5000。
- 如权利要求4所述的生物传感器电极,其特征是,所述单链DNA序列2由5’至3’的序列为TTTTTT CAC TAC AGA GGT TGC GTC TGT CCC ACG TTG TCA TGG GGG GTT GGC CTG。
- 一种权利要求4或5所述的生物传感器电极的制备方法,其特征是,将金纳米颗粒分散液滴加至玻碳电极表面使金纳米颗粒附着在玻碳电极表面,通过酰胺反应将至少含有两个氨基的分子连接至金纳米颗粒,再通过酰胺反应使羧基封端的聚N-异丙基丙烯酰胺与至少含有两个氨基的分子连接,然后通过酰胺反应使生物识别分子2与羧基封端的聚N-异丙基丙烯酰胺连接;优选的,制备方法中的涉及的反应温度、处理温度为室温或者37±0.5℃。
- 一种电致化学发光的生物传感器,其特征是,包括权利要求1或2所述的探针和权利要求4或5所述的生物传感器电极。
- 一种电致化学发光的试剂盒,其特征是,包括权利要求1或2所述的探针、权利要求4或5所述的生物传感器电极及鲁米诺。
- 一种权利要求1或2所述的探针、权利要求4或5所述的生物传感器电极、权利要求7所述的生物传感器或权利要求8所述的试剂盒在电致化学发光检测外泌体中的应用。
- 一种电致化学发光检测外泌体的方法,其特征是,将权利要求4或5所述的生物传感器电极浸没至待测外泌体溶液中,使外泌体附着在生物传感器电极上,然后将附着外泌体生物传感器电极浸没至权利要求1或2所述探针的溶液中,使探针附着在生物传感器电极的外泌体上,从而组成探针和生物传感器电极夹载外泌体的生物传感器,对探针和生物传感器电极夹载外泌体的生物传感器进行电化学发光检测即可。
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KR1020197025476A KR102209124B1 (ko) | 2018-04-20 | 2018-11-26 | Ti₃C₂2차원 금속 탄화물 촉매를 이용한 루미놀 전기화학발광 프로브 기반의 바이오 센서 및 그 제조 방법 |
US16/607,911 US20210102900A1 (en) | 2018-04-20 | 2018-11-26 | Biosensor based on trititanium dicarbide two-dimensional metal carbide catalyzed luminol electrogenerated chemiluminescence probe and preparation method |
JP2019547281A JP6796231B2 (ja) | 2018-04-20 | 2018-11-26 | Ti3C2二次元金属炭化物触媒に基づくルミノールの電気化学発光プローブを用いたバイオセンサー及びその製造方法 |
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CN107446797B (zh) * | 2016-05-31 | 2020-07-31 | 深圳汇芯生物医疗科技有限公司 | 外泌体处理芯片及处理方法 |
CN108562573B (zh) * | 2018-04-20 | 2020-01-03 | 青岛大学 | 一种基于二碳化三钛二维金属碳化物催化鲁米诺电化学发光探针的生物传感器及制备方法 |
CN109490284B (zh) * | 2018-12-03 | 2020-06-19 | 青岛大学 | 一种基于金纳米颗粒和二碳化钛MXenes的双重催化鲁米诺电化学发光生物传感器 |
CN109825293B (zh) * | 2019-01-30 | 2021-04-16 | 吉林大学 | 碳化钛纳米片作为上转换材料的应用 |
CN110320260B (zh) * | 2019-07-27 | 2021-07-30 | 福建师范大学 | 基于MXenes和黑磷量子点增强的外泌体电致化学发光传感器 |
CN115247058A (zh) * | 2021-04-28 | 2022-10-28 | Tcl科技集团股份有限公司 | 一种复合材料及其制备方法、量子点发光二极管及其制备方法 |
CN113702453B (zh) * | 2021-06-03 | 2023-05-09 | 江苏大学 | 一种AuNPs-p-Ti3C2Tx复合材料及其制备方法和应用 |
CN113340881B (zh) * | 2021-06-04 | 2023-04-25 | 安徽师范大学 | 目标物和氧化还原双响应适配体传感器及其制备方法和应用以及鱼腥藻毒素的定量检测方法 |
CN114088793A (zh) * | 2021-08-31 | 2022-02-25 | 复旦大学附属儿科医院 | 一种有机电化学晶体管传感器及其制备方法与应用 |
CN113758986B (zh) * | 2021-09-14 | 2024-04-16 | 湖北大学 | 基于Ti3C2-MXene沟道的电化学晶体管传感器及制备方法、检测亚硝酸盐的方法 |
CN113817230A (zh) * | 2021-09-30 | 2021-12-21 | 南京林业大学 | 一种CNF-MXene-PEI高强度高导电材料及其制备方法和应用 |
CN114235916B (zh) * | 2021-11-26 | 2022-10-21 | 华南理工大学 | 电化学生物传感器及其制备方法与应用 |
CN117288816A (zh) * | 2023-08-01 | 2023-12-26 | 广东工业大学 | 一种基于MXene-DNA的复合材料、传感器及其制备方法和在检测阿霉素中的应用 |
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KR102209124B1 (ko) | 2021-01-28 |
CN108562573B (zh) | 2020-01-03 |
CN108562573A (zh) | 2018-09-21 |
JP2020522678A (ja) | 2020-07-30 |
US20210102900A1 (en) | 2021-04-08 |
JP6796231B2 (ja) | 2020-12-09 |
KR20190126786A (ko) | 2019-11-12 |
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