WO2018100756A1 - Procédé de production d'un extrait de placenta oléosoluble - Google Patents

Procédé de production d'un extrait de placenta oléosoluble Download PDF

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Publication number
WO2018100756A1
WO2018100756A1 PCT/JP2017/007835 JP2017007835W WO2018100756A1 WO 2018100756 A1 WO2018100756 A1 WO 2018100756A1 JP 2017007835 W JP2017007835 W JP 2017007835W WO 2018100756 A1 WO2018100756 A1 WO 2018100756A1
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WIPO (PCT)
Prior art keywords
placenta
oil
extraction
heating
extract
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PCT/JP2017/007835
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English (en)
Japanese (ja)
Inventor
三井　幸雄
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株式会社ホルス
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Publication date
Application filed by 株式会社ホルス filed Critical 株式会社ホルス
Priority to KR1020177020363A priority Critical patent/KR101963064B1/ko
Priority to CN201780000668.8A priority patent/CN108472241B/zh
Publication of WO2018100756A1 publication Critical patent/WO2018100756A1/fr

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/98—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin
    • A61K8/981—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin of mammals or bird
    • A61K8/982—Reproductive organs; Embryos, Eggs
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/48—Reproductive organs
    • A61K35/50—Placenta; Placental stem cells; Amniotic fluid; Amnion; Amniotic stem cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/98—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A61P17/16—Emollients or protectives, e.g. against radiation
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q1/00—Make-up preparations; Body powders; Preparations for removing make-up
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin

Definitions

  • the present invention relates to a method for producing an oil-soluble placenta extract that can be incorporated into oily cosmetics such as foundations, lipsticks, oils, cleansings, or quasi drugs.
  • Placenta extract is widely used as a raw material for placenta-derived cosmetics.
  • most of the placenta blended in quasi drugs and cosmetics on the market are water-soluble extracts.
  • Water-soluble placenta extract cannot be blended in oil-based dosage forms such as lip balm, foundation and oil cosmetics.
  • Patent Document 1 includes a placenta extract extracted from the placenta, an oil and fat, and an additive consisting of at least one selected from the group consisting of collagens, algae, plant extracts and royal jellys.
  • An aged rejuvenation nutritional supplement is disclosed.
  • Patent Document 2 discloses a hair styling composition comprising hydrophobic silica, a dispersion liquid in which hydrophobic silica is dispersed, and a hair-growth component, and a placenta extract can be used as the hair-growth component. It is described that can be an oil-soluble substance.
  • Patent Document 3 includes (A) black swamp leaf (Morus Nigra Leaf), bouncy hunka flower (Magnolia Biondii Flower), red clover (Trifolium Pratense), white rose lupine seed (Lupinus Albus Seed), Hypericum Perforatum, and mint mint.
  • One or more extractions selected from the group consisting of flowers (Origanum Vulgare Flower), pansy (Viola Tricolor), cornflower (Centaurea Cyanus Flower), Melissa (Melissa Officinalis), and calendula officinalis flower And / or (B) an oil agent purified by column adsorption purification and / or (C) a cosmetic composition containing a whitening component, wherein olive oil can be used as the oil agent, and placenta extract as the whitening component. It is described that it can be used.
  • Patent Document 4 discloses a percutaneous absorption type pregnancy line formation inhibitor containing whey, a fibroblast formation promoting component, and an oil, and uses placenta extract as a fibroblast formation promoting component. It is described that can be.
  • JP 2016-113442 A International Publication No. 2012/105096 JP 2008-50292 A JP 2002-145788 A
  • Patent Document 1 to Patent Document 4 have a description that the oil and the placenta extract are blended, there is no description about a specific method for producing the oil-soluble placenta extract.
  • an object of the present invention is to provide a method for producing an oil-soluble placenta extract that contains a phosphorus-containing component and can be blended in oily cosmetics or quasi drugs.
  • the method for producing the oil-soluble placenta extract of the present invention according to claim 1 includes a shredding step for shredding the placenta, a heating step for heating the shredded placenta after the shredding step, and the heating step.
  • a freeze-drying step of freeze-drying the placenta, a powdering step of powdering the freeze-dried placenta after the freeze-drying step, and a powdered placenta after the powdering step An extraction step in which ether is added for extraction, a removal step for removing other than the ether fraction from the extract after the extraction step, and after the removal step, the extract is dried under reduced pressure to contain phosphorus.
  • the present invention according to claim 2 is the method for producing an oil-soluble placenta extract according to claim 1, wherein, in the extraction step, the extraction temperature is 20 ° C. or more and 60 ° C. or less, and the amount of the ether added is pulverized. The weight of the placenta is 5 to 10 times the weight.
  • the extraction temperature is 40 ° C.
  • a heating temperature is set to 110 ° C or higher and 135 ° C or lower
  • the ratio of the dried product to be dissolved in the oil is 0.1% to 5.0%.
  • the heating temperature in the heating step is 120 ° C.
  • the heating time is 20 minutes to 30 minutes
  • the manufacturing method of the oil-soluble placenta extract according to the first embodiment of the present invention includes a shredding step for shredding the placenta, a heating step for heating the shredded placenta after the shredding step, and a heating step Later, the freeze-drying process of freeze-drying the placenta, the powdering process of lyophilized placenta after the freeze-drying process, and after the powdering process, extraction is performed by adding ether to the powdered placenta.
  • the extraction step to be performed the extraction step after the extraction step to remove the ether fraction other than the ether fraction, and after the removal step, the extract is dried under reduced pressure to obtain a dried product containing phosphorus-containing components
  • an oil dissolving step of dissolving the dried product in oil is provided.
  • an oil-soluble placenta extract containing a predetermined amount or more of a phosphorus-containing component can be produced.
  • the placenta tissue is changed, and the extraction efficiency of the phosphorus-containing component is improved.
  • the extraction temperature is 20 ° C. or higher and 60 ° C. or lower, and the amount of ether added is powdered.
  • the placenta weight is 5 times to 10 times the weight. According to the present embodiment, the phosphorus-containing component can be extracted more efficiently.
  • the third embodiment of the present invention is an oil-soluble placenta extract production method according to the second embodiment, wherein the extraction temperature is 40 ° C. or higher and 60 ° C. or lower, and the extraction time is 2 hours or shorter. According to the present embodiment, the phosphorus-containing component can be extracted more efficiently, and alteration of the phosphorus-containing component can be avoided.
  • the heating temperature is set to 110 ° C. or higher and 135 ° C. or lower.
  • the ratio of the dried product to be dissolved in the oil is 0.1% to 5.0%. According to this embodiment, an oil-soluble placenta extract containing 0.1 to 0.6% of a phosphorus-containing component can be produced.
  • FIG. 1 is a production process diagram of an oil-soluble placenta extract according to this example.
  • the manufacturing method of the oil-soluble placenta extract according to the present embodiment includes a placenta selection step 10, a shredding step 20, a heating step 30, a freeze-drying step 40, a powdering step 50, an extraction step 60, and a removal step 70. And a vacuum drying step 80 and an oil dissolution step 90.
  • a placenta as a raw material for the oil-soluble placenta extract is selected.
  • the placenta the placenta of mammals such as pigs, horses, and sheep can be used. Among them, since the placenta of pigs is particularly excellent in safety, the placenta of pigs is selected in this embodiment.
  • the skin tissue is removed from the placenta selected in the placenta selection step 10, and the villous tissue is shredded into a minced shape.
  • the placenta shredded in the shredding step 20 is heated.
  • high-pressure steam having a temperature of 110 ° C. or higher and 135 ° C. or lower is used in a high-pressure environment.
  • an apparatus that heats while applying pressure such as an autoclave, can be used.
  • the heat treatment in the heating step 30 improves the extraction efficiency of the phosphorus-containing component in the extraction step 60.
  • the oil-soluble placenta extract is an extract from a natural product, but due to its nature, a sterilization process such as heating and filtration cannot be set in a subsequent process like the water-soluble placenta extract.
  • the heating temperature in the heating step 30 is 110 ° C. or higher and 135 ° C. or lower, and the heating time is 15 minutes or longer and 1 hour or shorter, which also serves as an appropriate sterilization treatment for the placenta.
  • the heating temperature is 120 ° C. or higher and 122 ° C. or lower, and the heating time is 20 minutes or longer and 30 minutes or shorter.
  • the placenta heated in the heating step 30 is freeze-dried. Freeze drying lowers the water content of the placenta. By lowering the water content of the placenta before the extraction step 60, separation work other than the ether fraction in the removal step 70 becomes easy.
  • the placenta freeze-dried in the freeze-drying step 40 is pulverized or the like.
  • the contact efficiency with ether can be increased.
  • extraction is performed by adding ether to the placenta powdered in the powdering step 50.
  • ether diethyl ether is preferably used from the viewpoint of extractability of the phosphorus-containing component.
  • each of the case where 5 times the amount of diethyl ether is added to the lyophilized placenta and the case where the amount of 10 times the amount of diethyl ether is added to the lyophilized placenta are as follows. Extraction was performed.
  • the extraction time is set to 2 hours or less when heating.
  • the lower limit of the extraction time is preferably 30 minutes or more, and more preferably 1 hour or more.
  • the extraction temperature when heating is preferably 40 ° C. or more and 60 ° C. or less. More preferably, the temperature is set to be at least 55 ° C.
  • the portions other than the diethyl ether fraction are removed from the extract extracted in the extraction step 60. Since the placenta was freeze-dried in the freeze-drying step 40 to reduce the water content of the placenta in advance, other than the diethyl ether fraction can be removed by a simple method such as filtration.
  • vacuum drying process 80 vacuum drying is performed on the filtrate obtained by the filtration in the removal process 70. Thereby, diethyl ether is removed, and a paste-like dried product containing a phosphorus-containing component is obtained.
  • FIG. 2 (a) shows the extraction temperature when the heating temperature in the heating step is 120 ° C., the heating time is 20 minutes to 30 minutes, and 5 times the weight of diethyl ether is added to the freeze-dried placenta in the extraction step. It is a figure which shows the amount of phosphorus containing components for every extraction time.
  • FIG. 2B shows the extraction temperature when the heating temperature in the heating step is 120 ° C., the heating time is 20 minutes to 30 minutes, and 10 times the weight of diethyl ether is added to the freeze-dried placenta in the extraction step. It is a figure which shows the amount of phosphorus containing components for every extraction time. In FIG. 2, the highest value is shown as “100” for the phosphorus-containing component amount of the paste-like dried product obtained in the reduced-pressure drying step.
  • condition (condition 5) of adding 10 times the weight of diethyl ether to the freeze-dried placenta and extracting for 16 hours is the most efficient, 55 ° C.
  • condition (condition 3) in which 5 times the weight of diethyl ether is added to the freeze-dried placenta and extracted for 2 hours is the most efficient.
  • Extraction under normal temperature conditions can also be performed under warming conditions depending on the manufacturing environment such as the manufacturing equipment because the amount of phosphorus-containing components is almost the same as under warming conditions by extending the extraction time. It is possible to select the extraction under normal temperature conditions.
  • the heating step 30 is omitted and the lyophilization step 40 is performed, and ether is added to the placenta pulverized through the powdering step 50 to extract phosphorus-containing components.
  • the extraction conditions were Condition 5 (10 times the weight, extraction temperature: normal temperature (25 ° C.), extraction time: 16 hours).
  • the amount of phosphorus-containing component is determined by performing the heat treatment in the heating step 30 in the heating step 30 at a heating temperature of 120 ° C. and a heating time of 20 minutes to 30 minutes, and performing the freeze drying step 40 and the powdering step 50.
  • the pasty dried product obtained in the vacuum drying step 80 is dissolved in oil.
  • the oil is preferably olive oil or sesame oil, but other oils can also be used. After the paste-like dried product is dissolved in olive oil, if there is a precipitate, it is appropriately removed by filtration or the like to complete an oil-soluble placenta extract.
  • the ratio of the dried product dissolved in the oil is 0.1% or more and 5.0% or less.
  • the amount of the phosphorus-containing component contained in the oil-soluble placenta extract can be set to 0.1 to 0.6%.
  • the amount of the phosphorus-containing component contained in the oil-soluble placenta extract should be 0.2 to 0.5%.
  • an ICP emission spectroscopic analyzer manufactured by Agilent Technologies was used, and the sample was dry ashed and extracted with dilute hydrochloric acid.
  • the oil-soluble placenta extract produced by the production method of the present invention can be incorporated into oily cosmetics such as foundations, lipsticks, oils, cleansings, and quasi drugs.

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • General Health & Medical Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Dermatology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Epidemiology (AREA)
  • Reproductive Health (AREA)
  • General Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pregnancy & Childbirth (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
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  • Virology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Toxicology (AREA)
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  • Cosmetics (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

L'invention concerne un procédé de production d'un extrait de placenta oléosoluble comprenant : une étape de hachage (20) consistant à hacher un placenta ; une étape de chauffage (30) consistant à chauffer le placenta haché après l'étape de hachage (20) ; une étape de lyophilisation (40) consistant à lyophiliser le placenta après l'étape de chauffage (30) ; une étape de réduction en poudre (50) consistant à réduire en poudre le placenta lyophilisé après l'étape de lyophilisation (40) ; une étape d'extraction (60) consistant à ajouter de l'éther au placenta réduit en poudre en vue d'effectuer une extraction après l'étape de réduction en poudre (50) ; une étape d'élimination (70) consistant à éliminer une fraction autre qu'une fraction éther d'une solution d'extrait après l'étape d'extraction (60) ; une étape de séchage à pression réduite (80) consistant à sécher la solution d'extrait à une pression réduite en vue de produire un matériau séché comportant un constituant comportant du phosphore après l'étape d'élimination (70) ; et une étape de dissolution dans de l'huile (90) consistant à dissoudre le matériau séché dans une huile après l'étape de séchage à pression réduite (80). Il devient possible de proposer un procédé de production d'un extrait de placenta oléosoluble qui comporte un constituant comportant du phosphore et qui peut être mélangé dans un produit cosmétique ou quasi-médicament à base d'huile.
PCT/JP2017/007835 2016-12-01 2017-02-28 Procédé de production d'un extrait de placenta oléosoluble WO2018100756A1 (fr)

Priority Applications (2)

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KR1020177020363A KR101963064B1 (ko) 2016-12-01 2017-02-28 유용성 플라센탈 엑기스의 제조 방법
CN201780000668.8A CN108472241B (zh) 2016-12-01 2017-02-28 油溶性胎盘提取物的制造方法

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JP2016-233850 2016-12-01
JP2016233850A JP6100965B1 (ja) 2016-12-01 2016-12-01 油溶性プラセンタエキスの製造方法

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CN (1) CN108472241B (fr)
TW (1) TWI648053B (fr)
WO (1) WO2018100756A1 (fr)

Citations (1)

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Publication number Priority date Publication date Assignee Title
JP2006149290A (ja) * 2004-11-30 2006-06-15 Masateru Egashira プラセンタ粉末配合食品

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JP2002145788A (ja) 2000-11-10 2002-05-22 Pigeon Corp 妊娠線形成抑制剤
CN100564518C (zh) * 2004-07-20 2009-12-02 成都军区昆明总医院 胎盘羊膜细胞提取物及其在间充质干细胞诱导分化中的应用
JP2008050292A (ja) 2006-08-24 2008-03-06 Croda Japan Kk 化粧料組成物
WO2011102684A2 (fr) * 2010-02-22 2011-08-25 영남대학교 산학협력단 Composition contenant des extraits de placenta
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KR101963064B1 (ko) 2019-03-27
KR20180080126A (ko) 2018-07-11
JP2018090515A (ja) 2018-06-14
CN108472241B (zh) 2021-02-09
TW201821090A (zh) 2018-06-16
JP6100965B1 (ja) 2017-03-22
TWI648053B (zh) 2019-01-21
CN108472241A (zh) 2018-08-31

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