WO2016088968A1 - 피부상태 개선 활성을 갖는 펩타이드 및 이의 용도 - Google Patents
피부상태 개선 활성을 갖는 펩타이드 및 이의 용도 Download PDFInfo
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- WO2016088968A1 WO2016088968A1 PCT/KR2015/007554 KR2015007554W WO2016088968A1 WO 2016088968 A1 WO2016088968 A1 WO 2016088968A1 KR 2015007554 W KR2015007554 W KR 2015007554W WO 2016088968 A1 WO2016088968 A1 WO 2016088968A1
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/04—Peptides having up to 20 amino acids in a fully defined sequence; Derivatives thereof
- A61K38/08—Peptides having 5 to 11 amino acids
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
- C07K7/04—Linear peptides containing only normal peptide links
- C07K7/06—Linear peptides containing only normal peptide links having 5 to 11 amino acids
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
- A61P1/02—Stomatological preparations, e.g. drugs for caries, aphtae, periodontitis
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P11/00—Drugs for disorders of the respiratory system
- A61P11/06—Antiasthmatics
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P19/00—Drugs for skeletal disorders
- A61P19/02—Drugs for skeletal disorders for joint disorders, e.g. arthritis, arthrosis
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/08—Antiallergic agents
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/81—Protease inhibitors
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
Definitions
- the present invention relates to peptides having skin condition improving activity and uses thereof.
- MMPs Matrix metalloproteinases
- JS endopeptidase enzymes capable of breaking down large biomolecules such as collagen, proteoglycans and gelatin, and are largely classified into collagenase, gelatinase, stromelysin and membrane-type MMP.
- MMPs are all expressed in the form of proenzymes and then activated by truncation (Bond, JS, et al., Int. J. Biochem ., 75, 565-574 (1985); Chen, JM, Chen, WT , Cell , 48, 193-203 (1987); Harris, ED et al., Coll Rel Res , 4, 493-512 (1984)).
- Collagenase acts on triple helix interstitial collagen and gelatin, and three types of fibroblast collagenase, neutrophil collagenase and collagenase-3 are known, and collagen fibrils of type I, II and III ( Collagen fibrils) have been reported (Goldberg, GI, et al, J. Biol. Chem. , 261, 6600-6605 (1986); Fini, ME, et al., Biochemistry , 26, 6155-6165 ( 1987)). It is also known that these three collagenases have at least about 50% sequence identity with each other (Borkakoti, et al., Nature Struct. Biol. , 1, 106-110 (1994); EMBO, J. , 13, 1263). -1269 (1994).
- MMPs are divided into subpeptide sites, catalytic sites and C-terminal buoys. After all MMPs are produced and secreted in an inactive manner, 80 amino acids of the N-terminal whole peptide region are cut and activated by removing the cysteine of the region having this PRCGVPD sequence (Van Wart, HE et al. , Proc. Natl. Acad . Sci . USA, 87, 5578-5582 (1990)). Activated MMPs are known to inhibit activity by binding to a natural inhibitor, the Missue Inhibitor of Matrix Metalloproteinase, which is regulated by catalytic sites (Murphy, et al., J. Niol. Chem).
- MMPs have substrate specificities and are expressed in metabolism when normal cells need to degrade extracellular matrix or other collagen structures.
- Diseases mediated by MMP include atherosclerosis, inflammatory diseases of the central nervous system, Alzheimer's disease, skin aging, rheumatoid arthritis, osteoarthritis, corneal ulcers, bone diseases, proteinuria, abdominal aortic aneurysm, degenerative cartilage loss due to traumatic joint injury, Myelin deprivation diseases of the nervous system, cirrhosis, renal glomeruli, immature rupture of the embryonic membrane, inflammatory bowel disease, periodontal disease, aging-related macular degeneration, diabetic retinopathy, proliferative vitreoretinopathy, immature retinopathy, cone cornea, Sjogren syndrome , Myopia, ocular tumor, corneal graft rejection, angiogenesis, cancer infiltration and metastasis.
- Rheumatoid arthritis and osteoarthritis are caused by autoimmune abnormalities, but as the disease progresses, the extracellular matrix of articular cartilage is destroyed.
- stromelysin has been recognized as a major enzyme and has been shown to play an important role in converting procollagenase into active collagenase. Therefore, arthritis progression can be prevented by inhibiting MMP activity, which is reported to be derived from microorganisms infiltrating leukocytes or fibroblasts or externally.
- collagenase secreted from stimulation of the inflammatory mediator and collagenase secreted from bacteria break down collagen, which is a substrate of periodontal tissue, cause gum retraction, and gradually progress to cause periodontal disease.
- the activity of fibroblast collagenase and stromelysin isolated from inflamed gums was confirmed, and the level of enzyme was correlated with the degree of gingivitis observed (Overall, CM et al., J. Periodontal Res . 22, 81-88 (1987).
- MMPs are associated with the development of several central nervous systems (CNS). MMPs are thought to be involved in the accumulation of amyloid beta proteins in Alzheimer's disease by introducing inflammatory monocytes into the central nervous system and destroying myelin or blood-brain barriers (Yong, VW, et al., Trends Neurosci 21 (2), 75-80 (1998). MMPs have also been reported to have higher concentrations in Alzheimer's disease than normal brains (Leake A, Morris CM, & Whateley J. Neurosci).
- gelatinase B levels in cerebrospinal fluid are associated with multiple sclerosis and other neurological disorders (Miyazaki, K, et al., Nature 362,839-841 (1993)). It has also been reported to contribute to the degradation and accumulation of amyloid beta protein (Backstrom JR, et al., J neurosci 16 (24), 7910-9 (1996).
- MMP induces skin aging, anti-wrinkle treatment and prevention can be expected, and MMP also promotes angiogenesis and cancer infiltration and metastasis by basal membrane degradation. Therefore, MMP plays an important role in the invasion and metastasis of cancer through the decomposition of the basement membrane, and there is a need for the development of a medicament capable of inhibiting the disease caused by a wide variety of diseases.
- inhibitors need to be safely used for long-term use, and thus can be used as an ideal therapeutic agent. Therefore, there is a demand for the development of a low toxicity formulation as an inhibitor of MMP activity.
- MMP inhibitors are being actively researched for the effective treatment of various diseases mediated by MMP, and the development of MMP inhibitors is effectively used for the treatment of various diseases.
- peptides having amino acid sequences of SEQ ID NO: 1 and SEQ ID NO: 2 exhibit MMP2 inhibitory activity and collagen degradation inhibitory activity and are effective in improving skin condition.
- the present invention has been completed by identifying that it can be usefully used.
- Another object of the present invention to provide a composition for improving skin conditions.
- Another object of the present invention to provide a pharmaceutical composition for the prevention or treatment of inflammatory diseases.
- Still another object of the present invention is to provide a method for preventing or treating an inflammatory disease.
- the present invention provides a peptide having skin condition improving activity consisting of the amino acid sequence of SEQ ID NO: 1 or SEQ ID NO: 2 sequence.
- peptides having amino acid sequences of SEQ ID NO: 1 and SEQ ID NO: 2 exhibit MMP2 inhibitory activity and collagen degradation inhibitory activity and are effective in improving skin condition. It was found that it can be usefully used.
- the peptide of the present invention comprises the amino acid sequence of SEQ ID NO: 1 or SEQ ID NO: 2 sequence. Specifically, the peptide of the present invention consists essentially of the amino acid sequence of SEQ ID NO: 1 or SEQ ID NO: 2.
- peptide refers to a linear molecule formed by binding amino acid residues to each other by peptide bonds.
- the peptides of the present invention may be prepared by chemical synthesis methods known in the art, in particular solid-phase synthesis techniques (Merrifield, J. Amer. Chem. Soc. 85: 2149-54 (1963); Stewart, et al., Solid Phase Peptide Synthesis , 2nd. Ed., Pierce Chem. Co .: Rockford, 111 (1984)) or liquid phase synthesis technology (US Pat. No. 5,516,891).
- the peptides of the present invention can induce modifications at the N-terminus or C-terminus to select some sites of the amino acid sequence and increase their activity. Through such modifications, the peptides of the present invention may have a high half-life which increases the half-life when administered in vivo.
- the C-terminus of the peptide of the present invention is modified with a hydroxy group (-OH), an amino group (-NH 2 ), azide (-NHNH 2 ), etc.
- the N-terminus of the peptide is an acetyl group, a fluorenyl methyl
- a protecting group selected from the group consisting of a methoxy carbonyl group, formyl group, palmitoyl group, myristyl group, stearyl group and polyethylene glycol (PEG) may be bonded.
- Modification of the above-mentioned amino acid serves to greatly improve the stability of the peptide of the present invention.
- stability herein means not only in vivo stability but also storage stability (eg, room temperature storage stability).
- the above protecting groups serve to protect the peptides of the present invention from the attack of protein cleavage enzymes in vivo.
- the peptide of the present invention not only has a function of directly inhibiting the activity of matrix metalloproteinase-2 (MMP2), but also has a function of inhibiting collagen degradation by MMP2.
- MMP2 matrix metalloproteinase-2
- the peptide of the present invention has an excellent inhibitory effect on melanosome transfer involved in skin whitening.
- a cosmetic composition for improving skin conditions comprising the peptide of the present invention as an active ingredient.
- composition of the present invention includes the above-described peptide of the present invention as an active ingredient, the common content between the two is omitted in order to avoid excessive complexity of the present specification.
- the improvement of the skin condition in the present invention is wrinkle improvement, skin elasticity improvement, skin aging prevention, skin moisturization improvement, wound removal, acne improvement, skin regeneration or whitening.
- the peptide of the present invention has a much lower molecular weight than other proteins, the skin penetration rate is very good. Therefore, when the composition of the present invention is applied to the skin topically, the skin condition can be effectively improved.
- the composition of the present invention comprises (a) a cosmetically effective amount of the above-described peptide of the present invention; And (b) a cosmetically acceptable carrier.
- cosmetic effective amount means an amount sufficient to achieve the skin improving efficacy of the composition of the present invention described above.
- Cosmetic compositions of the present invention may be prepared in any formulation conventionally prepared in the art, for example, solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing , Oils, powder foundations, emulsion foundations, wax foundations and sprays, and the like, but are not limited thereto. More specifically, it may be prepared in the form of a flexible lotion, nutrition lotion, nutrition cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray or powder.
- the formulation of the present invention is a paste, cream or gel, animal oils, vegetable oils, waxes, paraffins, starches, trachants, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicas, talc or zinc oxide may be used as carrier components.
- animal oils, vegetable oils, waxes, paraffins, starches, trachants, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicas, talc or zinc oxide may be used as carrier components.
- animal oils vegetable oils, waxes, paraffins, starches, trachants, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicas, talc or zinc oxide
- cellulose derivatives polyethylene glycols
- silicones bentonites
- silicas talc or zinc oxide
- lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used, in particular in the case of a spray, additionally chlorofluorohydrocarbon, propane Propellant such as butane or dimethyl ether.
- a solvent, solubilizer or emulsifier is used as the carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 Fatty acid esters of, 3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan.
- liquid carrier diluents such as water, ethanol or propylene glycol
- suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystals Soluble cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.
- the carrier component is an aliphatic alcohol sulfate, an aliphatic alcohol ether sulfate, a sulfosuccinic acid monoester, an isethionate, an imidazolinium derivative, a methyltaurate, a sarcosinate, a fatty acid amide.
- Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.
- the components included in the cosmetic composition of the present invention include components conventionally used in cosmetic compositions, in addition to the peptide and carrier components as active ingredients, and include, for example, conventional agents such as antioxidants, stabilizers, solubilizers, vitamins, pigments and flavorings. Adjuvants may be included.
- the present invention is a pharmaceutical composition for the prevention or treatment of MMP-activity-related diseases comprising the peptide of the present invention, the MMP-activity-related diseases are arthritis, diabetic retinopathy, It provides a composition characterized in that it is hypertrophic scars, psoriasis, ulcers of mucous membranes and epithelial tissue, inflammation caused by autoimmunity, lupus, autoimmune neuropathy, muscle cell destruction, glaucoma or excessive angiogenesis associated with the degradation of the basement membrane.
- the peptide of the present invention can be usefully used for treating diseases related to this by exhibiting various physiological activities such as inhibiting activity of MMP2, inhibiting collagen degradation, and inhibiting melanosomal migration.
- the composition of the present invention comprises (a) a pharmaceutically effective amount of the above-described peptide of the present invention; And (b) a pharmaceutically acceptable carrier.
- the term “pharmaceutically effective amount” means an amount sufficient to achieve the efficacy or activity of the peptides described above.
- Pharmaceutically acceptable carriers included in the pharmaceutical compositions of the present invention are those commonly used in the preparation, such as lactose, dextrose, sucrose, sorbitol, mannitol, starch, acacia rubber, calcium phosphate, alginate, gelatin, Calcium silicate, microcrystalline cellulose, polyvinylpyrrolidone, cellulose, water, syrup, methyl cellulose, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil, and the like It doesn't happen.
- the pharmaceutical composition of the present invention may further include a lubricant, a humectant, a sweetener, a flavoring agent, an emulsifier, a suspending agent, a preservative, and the like.
- a lubricant e.g., talc, kaolin, kaolin, kaolin, kaolin, kaolin, kaolin, kaolin, kaolin, kaolin, a kaolin, sorbitol, sorbitol, sorbitol, sorbitol, sorbitol, sorbitol, sorbitol, sorbitol, sorbitol, sorbitol, sorbitol, sorbitol, sorbitol, mannitol, mannitol, mannitol, mannitol, mannitol, mannitol, mannitol, mannitol, mannitol, mann
- composition of the present invention may be administered orally or parenterally, preferably parenterally, and in the case of parenteral administration, may be administered by intramuscular injection, intravenous injection, subcutaneous injection, intraperitoneal injection, topical administration, transdermal administration, or the like. Can be.
- Suitable dosages of the pharmaceutical compositions of the present invention may vary depending on factors such as the formulation method, mode of administration, age, weight, sex, morbidity, condition of food, time of administration, route of administration, rate of excretion and response to response of the patient. Can be. On the other hand, the preferred dosage of the pharmaceutical composition of the present invention is 0.0001-1000 ⁇ g per day.
- compositions of the present invention may be prepared in unit dose form by formulating with a pharmaceutically acceptable carrier and / or excipient according to methods which can be easily carried out by those skilled in the art. Or may be prepared by incorporating into a multi-dose container.
- the formulation may be in the form of a solution, suspension or emulsion in an oil or an aqueous medium, or may be in the form of extracts, powders, granules, tablets or capsules, and may further include a dispersant or stabilizer.
- the present invention provides a pharmaceutical composition for the prevention or treatment of inflammatory diseases comprising the peptide of the present invention as an active ingredient.
- the inflammatory disease to which the composition of the present invention can be applied is periodontitis, asthma, eczema, psoriasis, allergy, rheumatoid arthritis, psoriatic arthritis, atopic dermatitis, acne, atopic rhinitis Hay fever), allergic dermatitis (eczema), chronic sinusitis or seborrheic dermatitis.
- the peptide of the present invention reduces the inflammatory response by inhibiting the expression of the pro-inflammatory cytokines Protease-activated receptor 2 (PAR2) and IL-1 ⁇ , which are involved in the inflammatory response, thereby preventing and treating inflammatory diseases related thereto. It can be usefully used.
- the present invention provides a method for improving skin condition comprising administering a peptide of the present invention to a subject.
- the present invention provides a method for preventing or treating an inflammatory disease comprising administering a peptide of the present invention to a subject.
- the present invention provides a peptide having skin condition improving activity.
- the peptide of the present invention exhibits very good efficacy in improving skin condition by inhibiting MMP2 activity, and the composition comprising the peptide of the present invention has excellent physiological activity such as inhibiting collagen degradation and inhibiting melanosomal migration. It can be used for wrinkle improvement, skin regeneration, skin elasticity improvement, skin aging inhibition, wound regeneration, acne improvement, skin regeneration or skin whitening.
- composition comprising the peptide of the present invention can be used as a pharmaceutical composition for the prevention or treatment of MMP-activity-related diseases and inflammatory diseases.
- Figure 2 is a result of evaluating the effect of promoting the proliferation of human primary dermal fibroblasts (Human primary dermal fibroblast cells) by the peptide of the present invention.
- Figure 3 shows the results of confirming the change in signaling by the peptide of the present invention in human primary dermal fibroblasts.
- 6 and 7 are the results of evaluating the collagen degradation inhibitory effect by the peptide of the present invention.
- Figure 9 is the result of evaluating the anti-inflammatory effect by CG-Noverin in the dental pulp stem cells (dental pulp stem cells).
- chloro trityl chloride resin CTL resin, Nova biochem Cat No. 01-64-0021
- MC methylene chloride
- DMF dimethylformamide
- 10 ml of dichloromethane solution was added to the reactor, 200 mmole of Fmoc-Gly-OH (Bachem, Swiss) and 400 mmole of diisopropyl ethylamine (DIEA) were added thereto, stirred to dissolve well, and reacted with stirring for 1 hour.
- Cys-Gly-CTL resin was prepared by deprotection twice in the same manner as above with the deprotection solution. After sufficient washing with DMF and MC, once again Kaiser test was performed, and the following amino acid attachment experiment was performed as above. Fmoc-Ile, Fmoc-Trp, Fmoc-Lys (Boc), Fmoc-Glu (OtBu), Fmoc-Ser (tBu), Fmoc-Ser (tBu) and Fmoc-Glu (OtBu) based on the selected amino acid sequence The chain reaction was carried out. The Fmoc-protector was reacted twice with a deprotection solution twice for 10 minutes and then washed well.
- Acetylation of acetic anhydride, DIEA and HoBt was performed for 1 hour, and then the prepared peptidyl resin was washed three times with DMF, MC, and methanol, and dried slowly by flowing nitrogen air, followed by vacuum under P 2 O 5 . After drying completely under reduced pressure, 30 ml of fugitive solution (Trifluroacetic acid 81.5%, distilled water 5%, Thianisole 5%, Phenol 5%, EDT 2.5%, and TIS 1%) 30 ml After putting in and shaking occasionally at room temperature to maintain a reaction for 2 hours. The resin was filtered off and the resin was washed with a small amount of TFA solution and then combined with the mother liquor.
- Peptide molecular weight of the first sequence is 1041.2 (theoretical value: 1041.25) and the peptide molecular weight of the second sequence is 1072.2 (theoretical value: 1072.27) when measured using a molecular weight measuring instrument.
- NIH3T3 epidermal growth factor
- CG-Renoin or CG-Noverin peptides were concentrated (1-10 ⁇ g / ml) After treatment and incubation in an incubator for 30 minutes at 37 °C UVA (8 J) irradiated and incubated for 24 hours.
- Cell lysis buffer was treated to ensure lysate and protein quantification.
- Western blot was performed using anti-pERK (Santa Cruz Biotechnology, USA) and p38 (Santa Cruz Biotechnology, USA) antibodies to confirm the expression of cytoactive factors.
- Example 3 CG- Renuin Or CG- To Noverin by rhMMP2 control
- rhMMP2 and peptides were mixed by concentration (0.1 ⁇ g / ml, 1 ⁇ g / ml) and incubated at room temperature for 1 hour, followed by gelatin zymography to examine their expression.
- gelatin (2 mg / ml) was used as a substrate for protein electrophoresis (SDS-PAGE). After electrophoresis, the gel was immersed in 2.5% Triton X-100 for 30 minutes, and again at 37 ° C. for 24 hours in a buffer composed of 50 mM Tris-HCl, 0.2 M NaCl, 5 mM CaCl 2 , 1% Triton X-100. Incubated at.
- the gel was stained with Coo-massie Brilliant Blue R250 (Sigma) and destained with a solution composed of 5% methanol, 7.5% acetic acid and distilled water. In addition, the band appeared by gelatin hydrolysis was observed.
- the active MMP-2 was found to be 66 kDa and pro-MMP-2 was found to be 72 kDa.
- Fibroblasts were seeded in 24-well plates at a cell density of 3 ⁇ 10 4 cells / well. Incubate 24 hours in serum-free medium the next day and induce the expression and activation of MMP2 with TNF- ⁇ and then intensify CG-Renuin or CG-Noverin by concentration (10 ng / ml, 100 ng / ml, 1000 ng / ml), respectively. After treatment, the cells were incubated for 24 hours. The supernatant obtained by centrifugation at 14,000 xg for 10 minutes was subjected to gelatin zymography and then subjected to protein electrophoresis (SDS-PAGE) using gelatin (2 mg / ml) as a substrate.
- SDS-PAGE protein electrophoresis
- the gel was immersed in 2.5% Triton X-100 for 30 minutes, and again at 37 ° C. for 24 hours in a buffer composed of 50 mM Tris-HCl, 0.2 M NaCl, 5 mM CaCl 2 , 1% Triton X-100. Incubated at. After incubation, the gel was stained with Coo-massie Brilliant Blue R250 (Sigma) and destained with a solution composed of 5% methanol, 7.5% acetic acid and distilled water. In addition, the band appeared by gelatin hydrolysis was observed. Active MMP-2 was found to be 66 kDa and pro-MMP-2 was found to be 72 kDa.
- Example 5 Inhibition of Collagen Degradation by CG-Renuin or CG-Noverin
- HDF Human dermal fibroblast
- HDF Human dermal fibroblast (HDF) cells (3x10 4 cells) were seeded in 24-well plates and replaced with 5% serum medium the next day and treated with IGF-1 (100 ng / ml) (Sigma / USA) for 44 hours. After treatment with MMP2 (20 ng / ml), CG-Noverin, CG-Renuin and incubated for 4 hours. The supernatant obtained by centrifugation at 14,000 xg for 10 minutes was analyzed using pro-collagen type I kit.
- the HaCaT keratinocytes were used for the pagocytosis assay.
- As a material for pagocytosis bioparticles with fluorescent materials were used.
- 96-well tissue culture plates were allowed to be 3 x 10 3 cells per well for 24 hours, followed by 6 hours in serum-free medium. Thereafter, 1 ⁇ g / ml of trypsin and 1 ⁇ g / ml of peptide were treated for 48 hours to induce pagocytosis.
- the live particles were pagocytosis into keratinocytes.
- the peptides of the present invention were treated with a concentration of 1 ⁇ g / ml for 4 hours and then incubated for 4 hours.
- mRNA was extracted from the cultured cells treated with the trigger and peptides, and reverse transcriptase polymerase chain reaction was performed using primers of PAR-2 and IL-1 ⁇ . The nucleotide sequence of each primer used is shown in Table 2.
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Abstract
Description
펩타이드 | 아미노산 서열 | 분석값(질량분석기) | |
분석치 | 이론치 | ||
서열 1 | CTKIYDPVC | 1041.2 | 1042.25 |
서열 2 | CPRHFNPVC | 1072.2 | 1072.27 |
IL-1β | 정방향 | 5’-CCGTGGACCTTCCAGGATCA-3’ |
역방향 | 5’-GATCCACACTCTCCAGCTGC-3’ | |
PAR2 | 정방향 | 5’-GGGTTTGCCAAGTAACGGC-3’ |
역방향 | 5’-GGGAACCAGATGACAGAGAGG-3’ |
Claims (11)
- 서열목록 제1서열 또는 서열목록 제2서열의 아미노산 서열로 이루어진 피부 상태 개선 활성을 갖는 펩타이드.
- 제 1 항에 있어서, 상기 펩타이드는 MMP2(matrix metalloproteinase-2)의 활성을 억제하는 것을 특징으로 하는 펩타이드.
- 제 1 항에 있어서, 상기 펩타이드는 콜라겐 분해를 억제하는 것을 특징으로 하는 펩타이드.
- 제 1 항에 있어서, 상기 펩타이드는 멜라노좀 이동(melanosome transfer)을 억제하는 것을 특징으로 하는 펩타이드.
- 제 1 항에 있어서, 상기 피부 상태 개선은 주름개선, 피부재생, 피부탄력 개선, 피부노화 억제, 상처 재생, 여드름 개선, 피부 재생 또는 피부 미백인 것을 특징으로 펩타이드.
- 제 1 항 내지 제 5 항 중 어느 한 항의 펩타이드를 유효성분으로 포함하는 피부 상태 개선용 조성물.
- 제 1 항 내지 제 5 항 중 어느 한 항의 펩타이드를 유효성분으로 포함하는 MMP-활성 관련 질환의 예방 또는 치료용 약제학적 조성물로서, 상기 매트릭스 MMP-활성 관련 질환은 관절염, 당뇨병성 망막증, 비대성 반흔, 건선, 점막 및 상피 조직의 궤양, 자가면역에 의한 염증, 기저막의 분해와 관련된 질병인 낭창, 자가 면역성 신경장애, 근세포 파괴, 녹내장 또는 과다한 혈관신생인 것을 특징으로 하는 조성물.
- 제 1 항 내지 제 5 항 중 어느 한 항의 펩타이드를 유효성분으로 포함하는 염증 질환의 예방 또는 치료용 약제학적 조성물.
- 제 8 항에 있어서, 상기 염증 질환은 치주염, 천식, 습진, 건선, 알러지, 류마티스 관절염, 건선 관절염(psoriatic arthritis), 아토피성 피부염, 여드름, 아토피성 비염(건초열), 알레르기성 피부염(습진), 만성 부비동염 또는 지루성 피부염(seborrheic dermatitis)인 것을 특징으로 하는 조성물.
- 제 1 항 내지 제 5 항 중 어느 한 항의 펩타이드를 대상(subject)에 투여하는 단계를 포함하는 피부 상태 개선 방법.
- 제 1 항 내지 제 5 항 중 어느 한 항의 펩타이드를 대상(subject)에 투여하는 단계를 포함하는 염증 질환의 예방 또는 치료 방법.
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US15/532,232 US10047125B2 (en) | 2014-12-05 | 2015-07-21 | Peptide having activity to improve skin condition and use thereof |
EP15864719.8A EP3228626B1 (en) | 2014-12-05 | 2015-07-21 | Peptide having activity to improve skin condition and use thereof |
ES15864719T ES2847623T3 (es) | 2014-12-05 | 2015-07-21 | Péptido que tiene actividad para mejorar el estado de la piel y uso del mismo |
CN201580066042.8A CN107001420B (zh) | 2014-12-05 | 2015-07-21 | 具有改善皮肤状态的活性的肽及其用途 |
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KR102265430B1 (ko) * | 2019-08-20 | 2021-06-15 | 주식회사 케어젠 | 피부 상태 개선 활성을 갖는 펩타이드 및 이의 용도 |
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KR102694595B1 (ko) * | 2023-02-21 | 2024-08-13 | 대봉엘에스 주식회사 | 피부 상태 개선 활성을 나타내는 신규 펩타이드를 포함하는 화장료 조성물 |
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CN107001420B (zh) | 2021-07-02 |
EP3228626B1 (en) | 2020-11-04 |
EP3228626A4 (en) | 2017-12-06 |
JP6567147B2 (ja) | 2019-08-28 |
JP2018197251A (ja) | 2018-12-13 |
JP2018505850A (ja) | 2018-03-01 |
US20170267721A1 (en) | 2017-09-21 |
US10487113B2 (en) | 2019-11-26 |
CN107001420A (zh) | 2017-08-01 |
EP3228626A1 (en) | 2017-10-11 |
US20190040100A1 (en) | 2019-02-07 |
ES2847623T3 (es) | 2021-08-03 |
JP6464266B2 (ja) | 2019-02-06 |
US10047125B2 (en) | 2018-08-14 |
KR101744959B1 (ko) | 2017-06-12 |
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