WO2016035221A1 - Extrait de pêche et son procédé de production - Google Patents

Extrait de pêche et son procédé de production Download PDF

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WO2016035221A1
WO2016035221A1 PCT/JP2014/080656 JP2014080656W WO2016035221A1 WO 2016035221 A1 WO2016035221 A1 WO 2016035221A1 JP 2014080656 W JP2014080656 W JP 2014080656W WO 2016035221 A1 WO2016035221 A1 WO 2016035221A1
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peach
extract
hexane
extraction
ethanol
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PCT/JP2014/080656
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English (en)
Japanese (ja)
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武蔵 岡安
耕平 黒田
淳子 山川
真起子 小林
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株式会社岡安商店
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Publication of WO2016035221A1 publication Critical patent/WO2016035221A1/fr

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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105—Plant extracts, their artificial duplicates or their derivatives
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L19/00—Products from fruits or vegetables; Preparation or treatment thereof
    • A23L19/09—Mashed or comminuted products, e.g. pulp, purée, sauce, or products made therefrom, e.g. snacks
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A61P17/16—Emollients or protectives, e.g. against radiation
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/08—Antiallergic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
    • A23L29/30—Foods or foodstuffs containing additives; Preparation or treatment thereof containing carbohydrate syrups; containing sugars; containing sugar alcohols, e.g. xylitol; containing starch hydrolysates, e.g. dextrin

Definitions

  • the present invention relates to a peach extract having active oxygen scavenging ability and containing glycosphingolipid (ceramide component), and a method for producing the same.
  • the present inventors also focused on useful trace components contained in by-products produced in the production process of edible rice bran oil made from vegetable oil and fat, especially rice bran, and as a result, a method for extracting and producing rice ceramide And has been providing naturally derived rice ceramide (sphingoglycolipid).
  • the rice ceramide provided by the present inventors is a ceramide derived from a natural material, and its safety is high and its purity is extremely high, so far, food, cosmetics, quasi drugs, and those It has been widely used as a material for processed products.
  • Ceramide is a kind of sphingolipid and is a generic name for a group of compounds in which sphingosine, which is a long-chain amino alcohol, and an fatty acid are amide-bonded.
  • This ceramide is known to exist in high concentrations in the cell membrane, and in particular, it exists as a main component of the skin keratinocyte lipid, and has a barrier function as a moisturizing function that prevents evaporation of moisture from inside the skin. I'm in charge.
  • ceramide in keratinocyte lipids decreases with aging and moisturizing ability declines, causing dry skin, wrinkles, or rough skin.
  • ceramide is insufficient as a cause of not only skin aging but also skin roughness and rough skin caused by atopic dermatitis. That is, dry skin in atopic dermatitis is originally characterized by low ceramide, and as the barrier function of the skin decreases, moisture tends to be lost from the inside of the skin to the outside. It has been reported that chemicals and chemicals can easily enter the skin, resulting in allergic reactions and various symptoms such as itching and inflammation.
  • Patent Document 1 various studies on plant-derived ceramide components such as various fruits or vegetables have been studied.
  • peach is said to have a high content of ceramide, and a technique for extracting ceramide from peach has been proposed (Patent Document 2).
  • Patent Document 2 Regarding the peach content, not only ceramide extraction but also the physiological activity of the peach fruit itself has been studied, and the polyphenol contained in the peach fruit and the active oxygen scavenging function of the peach itself have also been reported.
  • the extraction of ceramide from peach described in Patent Document 2 described above is the preparation of a glucosylceramide fraction by an extraction method using a chloroform / methanol mixture as an extraction solvent, taking into account the toxicity of chloroform and methanol used as the extraction solvent. In this case, it is not preferable from the viewpoint of safety. Therefore, examination of the extraction method of the ceramide from the peach which can ensure more safety is requested
  • Examples of techniques for extracting and producing ceramide components from plant components such as various fruits and vegetables that have been proposed so far include, for example, alcohol-based solvents (for example, grapes, cherries, wheat, rice, soybeans, etc.) 2) selected from water, ethanol, acetone, hexane, and a method of extracting the rice bran with ethanol after degreasing and pretreating the camellia rice cake with hexane (Patent Document 1) Extraction method using a mixed solvent of at least seeds (Patent Document 4), after hydrolyzing plant materials such as wheat, soybeans, corn, rice bran, etc. with an alkaline ethanol solvent, extraction method using a mixed solvent of hexane, acetone, and water (Patent Document 5 and Patent Document 6) are known.
  • alcohol-based solvents for example, grapes, cherries, wheat, rice, soybeans, etc.
  • Patent Document 4 Extraction method using a mixed solvent of at least seeds
  • Patent Document 5 and Patent Document 6 extraction method using a
  • the present inventors also tried the extraction methods described in the above-mentioned patent documents in order to extract the ceramide component from peach, but were not satisfied. Therefore, in view of the present situation, as a result of examining a method for efficiently and safely extracting a ceramide component from peach, the peach raw material was extracted with ethanol or an ethanol / hexane mixture, and the obtained extract was further diluted with hexane / water. It has been found that a peach extract having an active oxygen scavenging ability and containing a glycosphingolipid (ceramide component) can be efficiently obtained by partition extraction, and the present invention has been completed.
  • the present invention provides a method for efficiently and safely extracting a peach extract having active oxygen scavenging ability obtained from peaches, and a method for extracting active peaches obtained by the production method, and a glycosphingolipid ( It is an object to provide a peach extract containing a ceramide component).
  • a peach processed peach, mature peach, or a processed peach residue containing fiber components obtained by removing seeds and fruit juice from the extracted peach or mature peach, ethanol, or ethanol /
  • the present invention is a peach extract whose active oxygen scavenging ability is DPPH (diphenylpicrylhydrazyl) activity.
  • DPPH diphenylpicrylhydrazyl
  • the present invention is a peach extract having a DPPH activity of 5% or more and a ceramide content of 0.5% or more.
  • the present invention is a method for producing a peach extract having active oxygen scavenging ability and containing a sphingolipid (ceramide component), specifically, a fruit peach, a mature peach, or Peach processing residue containing fiber components obtained by removing seeds and fruit juice from plucked peach or mature peach is extracted with ethanol or ethanol / hexane mixture, and the resulting extract is further partitioned and extracted with hexane / water And a method for producing a peach extract containing a sphingoglycolipid (ceramide component).
  • ceramide component specifically, a fruit peach, a mature peach, or Peach processing residue containing fiber components obtained by removing seeds and fruit juice from plucked peach or mature peach is extracted with ethanol or ethanol / hexane mixture, and the resulting extract is further partitioned and extracted with hexane / water
  • the volume ratio of the extract used for extracting the peach processing residue and the hexane and water used for the partition extraction of the extract is 1: 0.5: 0.5 to 1: 2: 2 is a peach extract, and a production method thereof.
  • the present invention is a powdery product obtained by mixing the above-mentioned extract with cyclodextrin, or a food, cosmetic, quasi-drug, or processed product thereof in the form of an aqueous solution.
  • a peach extract having active oxygen scavenging ability can be obtained more efficiently than a peach raw material by a relatively simple method with excellent safety.
  • the method for producing a peach extract provided by the present invention is not limited to the point that an extract of ethanol or an ethanol / hexane mixture, ie, a so-called primary extract, is further partitioned and extracted with hexane / water. This is a manufacturing method that has not been studied and is extremely specific.
  • the peach extract thus produced contains ceramide (sphingoglycolipid), polyphenol and glycoside, has active oxygen scavenging ability, high safety, food, cosmetics or pharmaceuticals. It is extremely useful as a raw material for quasi-drugs and processed products.
  • the present invention extracts a peach processed residue containing a fiber component obtained by removing seeds and fruit juice from a fruit peach, mature peach, or fruit peach or mature peach, which is a peach raw material, with ethanol or an ethanol / hexane mixture.
  • a peach extract characterized by having an active oxygen scavenging ability obtained by further partitioning and extracting the extract with hexane / water and containing a glycosphingolipid (ceramide component).
  • the fruit peach used as a raw material is a fruit peach (young peach) by pruning in the peach production process, and the mature peach is a peach suitable for being shipped to the market as a peach.
  • a peach processing residue is a peach processing residue containing the fiber component which remove
  • the peach harvest is about 150,000 tons annually nationwide, and the fruit peach (young peach) produced during the harvesting process is said to be about 15,000 tons, which is about 10% of the peach harvest.
  • a small part of this fruit peach is processed for food such as pickles, but most of it is used as fertilizer as it is without being processed. There was no means for effective use.
  • peach fruit juice peach juice
  • about 1500 tons of peach processing residue is produced as a by-product per year, but it is discarded without any effective use.
  • a peach extract is produced by a specific combination of extraction solvents from fruit peaches that have been discarded without being effectively used, or peach processing residues, and by-products are effectively used. It is very specific in terms.
  • peaches containing the flesh and fruit skins from which the seeds have been removed from these fruit peaches (young peaches) or mature peaches are required. If there is, it can be used as a raw material for extraction after pre-freezing, finely pulverizing with a mixer or the like, and dried by hot air drying, reduced pressure drying, or reduced pressure drying.
  • the primary extraction solvent used in the production of a peach extract from these peach raw materials provided by the present invention is characterized in that ethanol alone or an ethanol / hexane mixture is used.
  • the peach extraction solvent disclosed in Patent Document 2 is a mixed solvent having a 1: 1 volume ratio of chloroform / methanol, and the obtained glucosylceramide fraction (extract) contains chloroform or methanol having high toxicity. Residuals are feared, and it is not necessarily preferable.
  • ethanol and hexane used in food processing and fat processing are used, and the safety of the obtained extract is extremely high.
  • the amount of extraction solvent used for the peach raw material that is the raw material for extraction, but it is better to use it in a conversion of about 0.1 to 1 L with respect to 100 g of the peach fine powder or peach processing residue as the raw material.
  • the mixing ratio is 1 to 5 volumes, preferably 1 to 3 volumes of ethanol with respect to 1 volume of hexane.
  • the extraction temperature and time in this case are not particularly limited, but it is preferable to carry out the extraction at a temperature of about room temperature to 80 ° C. for about 0.1 to 24 hours.
  • the extraction process which is a method for producing the peach extract of the present invention
  • primary extraction is performed with ethanol alone or a mixture of ethanol / hexane.
  • hydrous ethanol can also be used.
  • a so-called primary extract extracted with ethanol alone or a mixed solution of ethanol / hexane is further subjected to partition extraction with hexane / water, so that A peach extract having an active oxygen scavenging ability and containing a glycosphingolipid (ceramide component) is obtained.
  • the volume ratio of the extraction liquid in the primary extraction and the hexane and water used for the partition extraction of the extraction liquid is 1: 0.5: 0.5 to 1: 2: 2.
  • the volume ratio of hexane and water in the hexane / water partition extraction with respect to the primary extract is 0.5 hexane. It is preferable that the amount be appropriately selected within the range of 0.5 to 2 volumes of water.
  • the degree of separation in partition extraction and the content of the ceramide component can be increased.
  • the emulsification is eliminated by treatment such as addition of salt, heating / cooling, or filtration using an inert carrier such as celite. It is known that it can be.
  • the adoption of these methods is also studied, and the emulsification is eliminated by treatment such as salting out, heating / cooling, or filtration with an inert carrier such as celite at a mixing ratio exhibiting an emulsified state. In some cases, it was possible.
  • the peach processing residue containing the fiber component obtained by removing seeds and juice from the young peach, mature peach, or young peach or mature peach to be extracted is used.
  • the pulverized and dried product can be degreased with hexane in advance, and this degreasing treatment reduces the lipid content in the peach extract, and as a result, the ceramide content ratio can be improved.
  • the extraction operation can be performed under moderate alkaline conditions, but the extraction operation under such alkaline conditions is not essential.
  • the extract has a high polyphenol content when extracted using ethanol alone as the extraction solvent. Therefore, the extraction conditions can be appropriately selected according to the intended use of the components contained in the extract, and in that respect, the method for producing a peach extract of the present invention is extremely specific. It can be said that there is.
  • the extract obtained by the above extraction treatment is concentrated under reduced pressure, and further dried under reduced pressure, whereby the peach extract targeted by the present invention can be obtained as a solid.
  • the peach extract thus produced contains ceramide (glycosphingolipid), polyphenol and glycoside, and has active oxygen scavenging ability due to the inclusion of the polyphenol and other antioxidant active substances.
  • the content of ceramide is high, and so-called “peach ceramide” can be efficiently applied to foods, cosmetics, quasi drugs, and processed products thereof.
  • the active oxygen scavenging ability is 5% or more as DPPH (diphenylpicrylhydrazyl) activity, and the ceramide content is 0.5% or more.
  • the peach extract is, for example, in the form of a powder mixed with cyclodextrin, or a solution dissolved in an aqueous solution, food, cosmetics, quasi drugs, and processed products thereof. Applies to
  • Test Example 1 Measurement of ceramide content
  • the ceramide content (purity) of the peach extract in the following examples was measured as follows. 1. As a measurement sample, a peach extract was dissolved in a chloroform / methanol (2: 1 v / v) mixed solution so as to be 20 mg / mL to obtain a sample solution. 2. For the standard product, use a matreya (USA) standard substance (purified soybean-derived product) and dissolve in a chloroform / methanol (2: 1 v / v) mixture so that the concentration is 2 mg / mL. Prepared as a standard solution. 3.
  • Test Example 2 Measurement of DPPH radical scavenging activity DPPH (diphenylpicrylhydrazyl) radical scavenging activity in the following Examples was measured as follows. 1. Each measurement sample was adjusted to 2 mg / mL with ethanol. 2. DPPH was adjusted to 0.4 mM with ethanol. 3. The measurement sample solution was prepared as follows. (1) Sample 2 mg / mL sample solution 2 mL mixed with DPPH solution 1 mL and ethanol 1 mL (2) Sample blank 2 mg / mL sample solution 2 mL mixed with ethanol 2 mL (3) Control 3 mL ethanol and DPPH solution 1 mL mixed (4) Control blank ethanol 4mL 4). After the measurement sample solution was prepared, the reaction was allowed to occur for 20 minutes while protected from light. After the reaction, the absorbance (OD) at 571 nm was measured with a spectrophotometer.
  • DPPH diphenylpicrylhydrazyl
  • Test Example 3 Content measurement of total polyphenols The content of total polyphenols in the following examples was measured as follows. 1. The measurement sample was adjusted to 10 mg / mL with 80% ethanol, and further diluted 10-fold with distilled water to 1.0 mg / mL. 2. A standard gallic acid was prepared as follows. 28ppm, 14ppm, 7ppm, 33.5ppm, 1.75ppm 3. A 10% aqueous sodium carbonate solution was prepared. 4). A 5-fold diluted phenol reagent was prepared with purified water. 5. 2 mL of 5-fold diluted phenol reagent and 2 mL of 10% aqueous sodium carbonate solution were added to 2 mL of each measurement sample, and the mixture was stirred and reacted for 1 hour. 6). The reaction solution was filtered and the absorbance was measured at 760 nm. 7). A calibration curve was prepared with gallic acid to determine the total polyphenol (total PP) content (mg / g).
  • Reference Example 1 Preparation of extract from peach processing residue by extraction with ethanol alone To 10 g of dried peach processing residue, 20 mL of 95% ethanol was added and extracted with stirring at 42 ° C for 30 minutes. The extract was filtered under reduced pressure to obtain an extract, while the extraction residue was again extracted in the same manner to obtain an extract. Both extracts were combined, concentrated under reduced pressure using an evaporator, and the residue was dried under reduced pressure at 60 ° C. to obtain 1.17 g of an orange solid.
  • Reference Example 2 Production of extract from peach processing residue by extraction with hexane alone 60 mL of hexane was added to 60 g of dried peach processing residue, followed by extraction (defatting) at 60 ° C. for 2 hours with stirring operation. The extract was filtered under reduced pressure, and 60 mL of hexane was used for eviction. The obtained filtrate was concentrated under reduced pressure, and the residue was dried under reduced pressure to obtain 0.3 g of a yellow starch candy.
  • Example 1 Production of extract from fruit peach (young peach) ⁇ Extraction solvent: ethanol alone, hexane / water partition extraction with alkali treatment> Frozen fruit peach (young peach) was thawed for 15 minutes in a microwave oven, finely pulverized with a mixer, and dried. To 50 g of this powder, 300 mL of 95% ethanol (containing 9.6 g of sodium hydroxide) and 37.5 mL of purified water were added and extracted at 42 ° C. for 30 minutes with stirring. The extract was filtered under reduced pressure, 300 mL of hexane and 150 mL of purified water were added to the filtrate, and the mixture was extracted by partitioning. The hexane layer was collected, the solvent was concentrated under reduced pressure using an evaporator, and the residue was dried under reduced pressure at 60 ° C. to obtain 160.7 mg of a green solid.
  • extraction solvent ethanol alone, hexane / water partition extraction with alkali treatment>
  • Example 2 Production of extract from fruit peach (young peach) ⁇ Extraction solvent: ethanol alone, hexane / water partition extraction, no alkali treatment> Frozen fruit peach (young peach) was thawed for 15 minutes in a microwave oven, finely pulverized with a mixer, and dried. 50 g of this powder was added with 300 mL of 95% ethanol and 37.5 mL of purified water, and extracted at 42 ° C. for 30 minutes with stirring. The extract was filtered under reduced pressure, 300 mL of hexane and 150 mL of purified water were added to the filtrate, and the mixture was extracted by partitioning. The hexane layer was collected, the solvent was concentrated under reduced pressure with an evaporator, and the residue was dried under reduced pressure at 60 ° C. to obtain 486.0 mg of a green solid.
  • Example 3 Production of extract from peach processing residue ⁇ After degreasing process Extraction solvent: Ethanol alone, hexane / water partition extraction>
  • the squeezed rice cake (peach processed residue) after producing peach juice was dried, and 800 mL of hexane was added to 200 g of the peach processed residue, followed by stirring at 70 ° C. for 4 hours for degreasing treatment.
  • the peach processing residue after the degreasing treatment was recovered by filtration under reduced pressure. 800 mL of 95% ethanol was added to the resulting defatted peach processed residue, extracted at 70 ° C. for 60 minutes, and filtered under reduced pressure to obtain an extract.
  • the extraction residue was treated again in the same manner to obtain an extract.
  • Example 4 Production of extract from peach processing residue ⁇ Extraction solvent: ethanol alone, hexane / water partition extraction with alkali treatment> To 100 g of the dried peach processing residue, 200 mL of 95% ethanol (containing 6.4 g of sodium hydroxide) and 25 mL of purified water were added, and the mixture was extracted with stirring at 42 ° C. for 30 minutes. The extract was filtered under reduced pressure to obtain an extract, and the extraction residue was similarly treated again to obtain an extract. Both extracts were combined, 400 mL of hexane and 200 mL of purified water were added, and the mixture was stirred and distributed for 1 hour. The hexane layer was collected, the solvent was concentrated under reduced pressure with an evaporator, and the residue was dried under reduced pressure at 60 ° C. to obtain 131.3 mg of an orange solid.
  • extraction solvent ethanol alone, hexane / water partition extraction with alkali treatment>
  • Example 5 Preparation of extract from peach processing residue ⁇ Extraction solvent: ethanol alone, hexane / water partition extraction, no alkali treatment> To 100 g of the undried peach processing residue, 200 mL of 95% ethanol and 25 mL of purified water were added, and extracted with stirring at 42 ° C. for 30 minutes. The extract was filtered under reduced pressure to obtain an extract, and the extraction residue was similarly treated again to obtain an extract. Both extracts were combined, 400 mL of hexane and 200 mL of purified water were added, and the mixture was stirred and distributed for 1 hour. The hexane layer was collected, the solvent was concentrated under reduced pressure using an evaporator, and the residue was dried under reduced pressure at 60 ° C. to obtain 172.1 mg of an orange solid.
  • extraction solvent ethanol alone, hexane / water partition extraction, no alkali treatment>
  • Example 6 Preparation of extract from peach processing residue ⁇ Extraction solvent: Ethanol / hexane mixture hexane / water partition extraction> To 100 g of the undried peach processing residue, 200 mL of 95% ethanol, 100 mL of hexane and 25 mL of purified water were added, and the mixture was extracted with stirring at 42 ° C. for 30 minutes. The extract was filtered under reduced pressure to obtain an extract, and 100 mL of hexane and 100 mL of purified water were added to the filtrate, and the mixture was stirred at 42 ° C. for 1 hour and partitioned and extracted with hexane / water. The hexane layer was collected, the solvent was concentrated under reduced pressure using an evaporator, and the residue was dried under reduced pressure at 60 ° C. to obtain 177.8 mg of an orange solid.
  • extraction solvent Ethanol / hexane mixture hexane / water partition extraction>
  • Example 7 Preparation of extract from peach processing residue ⁇ Extraction solvent: ethanol alone, hexane / water partition extraction> To 50 g of the dried peach processing residue, 200 mL of 95% ethanol was added, and extracted with stirring at 42 ° C. for 30 minutes. The extract was filtered under reduced pressure, and 50 mL of 95% ethanol was used to drive out the extract. The residue was extracted again in the same manner, filtered under reduced pressure, and expelled. 150 mL of hexane was added to the combined filtrate (150 mL) and stirred for 30 minutes, and then 150 mL of distilled water was added and stirred for 30 minutes to perform partition extraction with hexane / water. The hexane layer was collected and concentrated under reduced pressure, and the residue was dried under reduced pressure to obtain 3.85 g of an orange-colored starch candy as an extract.
  • extraction solvent ethanol alone, hexane / water partition extraction>
  • Example 8 Examination of partition extraction conditions of hexane / water for peach raw material extract Extraction of hexane / water to a primary extract obtained by degreasing a dried peach processed residue with hexane and extracting with 95% ethanol The ratio of the solvent capacity was examined at the solvent ratio (primary extract: hexane: water) described in Table 1 below. The primary extract was added to the test tube, then hexane was added and shaken vigorously for 10 seconds, then water was added, shaken vigorously again for 10 seconds, and then allowed to stand to examine the state of separation of the solution.
  • a peach extract having an active oxygen scavenging ability could be efficiently obtained by using a specific extraction method from a peach raw material according to the present invention.
  • the obtained extract has not only active oxygen scavenging ability but also contains peach ceramide, and is highly safe in that ethanol and hexane are used as the extraction solvent.
  • peach ceramide contains peach ceramide, and is highly safe in that ethanol and hexane are used as the extraction solvent.
  • a peach extract containing ceramide (glycosphingolipid), glycoside, and polyphenol and having active oxygen scavenging ability can be produced from peach by a safe extraction method.
  • the peach extract obtained by the present invention can obtain a peach extract in which the content of the target component is changed by various combinations of extraction solvents used for the extraction, for example, the content of peach ceramide
  • the raw material is a peach processing residue generated from processing processes such as fruit peach (young peach) or peach juice produced in the peach production process, It is extremely specific in that by-products can be used effectively.

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Abstract

L'objet de cette invention est de pourvoir à un extrait de pêche obtenu à partir de pêches, ledit extrait de pêche ayant une capacité d'absorption d'oxygène actif, et de pourvoir à un procédé très sûr pour le produire. L'invention concerne un extrait de pêche ayant une capacité d'absorption d'oxygène actif qui est obtenu par : extraction d'un matériau de pêche de départ à l'aide d'éthanol ou d'un mélange éthanol/hexane, ledit matériau de pêche de départ comprenant des pêches cueillies (jeunes pêches), des pêches mûries ou un résidu qui est obtenu suite à un traitement des pêches et contenant les composants fibreux qui subsistent après l'élimination des graines et du jus des pêches cueillies ou des pêches mûries, et extraction par division de l'extrait primaire à l'aide d'un mélange hexane/eau. L'extrait de pêche contient des céramides (sphingoglycolipides) et des polyphénols. Un procédé de production d'extrait de pêche est en outre décrit. L'extrait de pêche obtenu dans la présente invention peut être utilisé à titre d'aliments, de produits cosmétiques, de quasi-médicaments et autres produits issus de leur transformation.
PCT/JP2014/080656 2014-09-03 2014-11-19 Extrait de pêche et son procédé de production WO2016035221A1 (fr)

Applications Claiming Priority (2)

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CN115299601A (zh) * 2022-06-20 2022-11-08 宁波大学 一种包埋桃皮渣多酚的高内相乳液保健品及其制备方法
CN115873672A (zh) * 2022-07-29 2023-03-31 江南大学 一种细菌生物膜清除剂及其制备方法

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CN113788864A (zh) * 2021-10-23 2021-12-14 绵阳膳意生物科技有限公司 一种神经酰胺和葡糖苷（脂）酰鞘氨醇的联产制备方法
CN115299601A (zh) * 2022-06-20 2022-11-08 宁波大学 一种包埋桃皮渣多酚的高内相乳液保健品及其制备方法
CN115299601B (zh) * 2022-06-20 2023-11-17 宁波大学 一种包埋桃皮渣多酚的高内相乳液保健品及其制备方法
CN115873672A (zh) * 2022-07-29 2023-03-31 江南大学 一种细菌生物膜清除剂及其制备方法

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