WO2015062550A1 - 对样本进行加热的方法 - Google Patents
对样本进行加热的方法 Download PDFInfo
- Publication number
- WO2015062550A1 WO2015062550A1 PCT/CN2014/090129 CN2014090129W WO2015062550A1 WO 2015062550 A1 WO2015062550 A1 WO 2015062550A1 CN 2014090129 W CN2014090129 W CN 2014090129W WO 2015062550 A1 WO2015062550 A1 WO 2015062550A1
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- WIPO (PCT)
- Prior art keywords
- nucleic acid
- heating
- plate
- deep
- acid extractor
- Prior art date
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/06—Hydrolysis; Cell lysis; Extraction of intracellular or cell wall material
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00584—Control arrangements for automatic analysers
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/0099—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor comprising robots or similar manipulators
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N2035/00178—Special arrangements of analysers
- G01N2035/00306—Housings, cabinets, control panels (details)
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N2035/00465—Separating and mixing arrangements
- G01N2035/00534—Mixing by a special element, e.g. stirrer
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N2035/1027—General features of the devices
- G01N2035/1048—General features of the devices using the transfer device for another function
- G01N2035/1053—General features of the devices using the transfer device for another function for separating part of the liquid, e.g. filters, extraction phase
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/0098—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor involving analyte bound to insoluble magnetic carrier, e.g. using magnetic separation
Definitions
- the invention relates to a method of heating a sample, in particular to a heating method in a nucleic acid extractor.
- nucleic acid molecules are the main research object, and the separation and linearization of nucleic acids is the basic technology of nucleic acid research.
- Nucleic acid is the basic unit representing the genetic characteristics of living organisms.
- Nucleic acid detection is an advanced biological test at the molecular level. It is more sensitive, specific, and windowless than traditional morphological, cytological, and immunological tests. Significant advantages such as the period.
- Nucleic acid detection includes qualitative PCR, molecular hybridization, real-time fluorescent quantitative PCR and other technologies.
- the primary key of these nucleic acid detection technologies is to complete the extraction of nucleic acids from biological samples. Therefore, the efficient and accurate extraction of nucleic acid templates has become the premise of subsequent nucleic acid detection.
- domestic nucleic acid extraction mainly adopts the traditional manual preparation method, which is not only inefficient, high in cost, but also has poor repeatability and stability.
- the first step is to add the sample to the deep well plate;
- the second step is to place the deep well plate at a designated position in the nucleic acid extractor;
- the third step is to trigger the micro switch on the nucleic acid extractor;
- Step the main control device of the nucleic acid extractor starts; the fifth step, the DC motor starts; the sixth step, the support portion of the nucleic acid extractor moves up; the seventh step, the clamp arm of the nucleic acid extractor clamps the deep well plate;
- the heating plate is closely attached to the deep hole plate; in the
- the deep well plate automatically triggers a microswitch on the nucleic acid extractor when the deep well plate is placed in a designated position within the nucleic acid extractor.
- the micro switch includes a tilted spring piece and a push button switch.
- the bottom of the deep hole plate pushes the spring piece to compress the push button switch, thereby opening the micro switch. , start the motor to work.
- a jig for fixing a deep-hole plate which comprises a motor located below the bottom plate of the nucleic acid extractor, a support portion located inside the operation table of the nucleic acid extractor, a plurality of clamp arms and a micro switch,
- the motor comprises a cam; when the motor is started, the cam pushes the support portion to move up and down; the support portion further pushes the clamp arm to rotate, the clamp arm clamps or releases the outer wall of the deep hole plate; and the torsion spring is arranged on the clamp arm; When the console is not placed with a deep hole plate, the torsion spring keeps the clamp arm released.
- a step of cooling is further included, which comprises using a heat sink disposed under the console of the nucleic acid extractor.
- the heat sink includes a cross flow fan that is hidden under the console.
- the heating device comprises a plurality of rows of movable heating plates disposed on the operation table of the nucleic acid extractor, each row of heating plates including a bottom shape of the deep plate to be heated A plurality of heating hole walls are closely matched, and the heating plate is made of a heat conductive material, and a heating member is inserted inside the heating plate, and a heat insulating member is disposed on the outer periphery thereof.
- a plurality of mutually independent compression springs are disposed under the heating plate, and the compression springs abut against the heating plate.
- the heating component is selected from the group consisting of a resistor, a resistance wire, a capacitor, an IC integrated circuit, a triode, and a ceramic.
- the invention has the beneficial effects that: the invention adopts a movable heating device, and a plurality of springs are arranged under the heating plate.
- the invention adopts a movable heating device, and a plurality of springs are arranged under the heating plate.
- the bottom of the deep hole plate presses the heating plate downward, so the two A good fit is formed between them. Therefore, each well is uniformly heated, the temperature difference is small, and the speed and degree of cracking of the sample in each well are more uniform than in the prior art.
- Figure 1 is a schematic illustration of a nucleic acid extractor of the present invention
- Figure 2 is a schematic view of Figure 1 after removing the outer casing and the base;
- Figure 3 is a schematic view of another perspective of Figure 2;
- Figure 4 is a schematic view of the nucleic acid extracting apparatus of the present invention after removing the inner and outer casings;
- Figure 5 is a schematic view of another perspective of Figure 4.
- Figure 6 is a schematic view of Figure 5 after removing parts
- Figure 7 is a schematic view of Figure 6 after removing parts
- Figure 8 is a schematic view similar to Figure 4, showing the nucleic acid extractor of the present invention in an initial state
- FIG. 9 is similar to FIG. 8 and is a schematic view of the nucleic acid extracting device of the present invention after being inserted into a magnetic sleeve;
- Figure 10 is similar to Figure 9 and is a schematic view of the nucleic acid extracting apparatus of the present invention after placing a deep-well plate;
- Figure 11 is a view similar to Figure 10, showing the magnetic rod of the nucleic acid extractor of the present invention after entering the deep well plate;
- Figure 12 is a plan view showing the magnetic rod sleeve and the magnetic rod of the nucleic acid extracting apparatus of the present invention in a first position after entering the deep hole plate;
- Figure 13 is similar to Figure 12, but the first motor group drives the second and third motor groups to move to the second position;
- Figure 14 is a schematic view showing the magnetic rod sleeve and the magnetic rod of the nucleic acid extracting apparatus of the present invention after adsorbing magnetic beads and nucleic acids;
- Figure 15 is a plan view showing the movement of the magnetic rod sleeve and the magnetic rod of the nucleic acid extracting instrument of the present invention.
- Figure 16 is a plan view similar to Figure 13, but with both the magnetic rod sleeve and the magnetic rod entering the second position of the deep orifice plate;
- Figure 17 is a plan view of the console
- Figure 17A is a partial enlarged view of the marked portion of Figure 17;
- Figure 18 is a plan view showing another angle of the console
- Figure 18A is a partial enlarged view of the marked portion of Figure 18;
- Figure 19 is a plan view similar to Figure 18, the switch is in a triggered state
- Figure 19A is a partial enlarged view of the marked portion in Figure 19;
- Figure 20-23 is a schematic view of the operation of the fixture
- Figure 24 is an enlarged schematic view of the power control component
- Figure 24A is a partial enlarged view of Figure 24;
- Figure 25 is an enlarged schematic view of the first motor unit
- Figure 26 is an enlarged schematic view of a third motor unit
- Figure 27 is a schematic view of the nucleic acid extracting apparatus of the present invention after removing parts
- Figure 27A is a partial enlarged view of Figure 27;
- Figure 28 is a schematic view showing the bottom plate and the operation table of the nucleic acid extracting instrument of the present invention.
- Figure 28A is a partial enlarged view of Figure 28;
- Figure 29 is a schematic view of Figure 28 after removing the operation panel
- Figure 30 is a schematic view of the bottom plate viewed from the bottom;
- Figure 31 is a schematic view of the principle of the present invention.
- Figure 32 is a schematic illustration of the door of the nucleic acid extractor of the present invention.
- Figure 32A is a partial enlarged view of Figure 32;
- Figure 33 is a partially enlarged schematic view showing the door of the nucleic acid extracting apparatus of the present invention.
- Figure 33A is a partial enlarged view of Figure 33;
- Figure 34 is a schematic view showing the operation of the nucleic acid extracting apparatus of the present invention.
- Figure 35 is a schematic illustration of the nucleic acid extractor of the present invention clamping a deep well plate and heating the deep well plate.
- the nucleic acid extractor 1 of the present invention comprises a base 10 at the bottom, an outer casing 20 above the base, an instrument body 30 located inside the outer casing and fixed to the base 10.
- the susceptor 10 is made of a material with a relatively high specific gravity and low cost (such as cast iron, steel, or filled with concrete), so that the pedestal has a large mass, thereby effectively preventing the nucleic acid extractor from occurring during operation. Vibration and displacement.
- the outer casing 20 is preferably made of a stainless steel plate of 0.1-2.0 mm thick by cold rolling and cold cutting. In other solutions, the outer casing 20 can also be made of a plastic material and manufactured by an injection molding process to reduce costs.
- a plurality of ribs or ribs which are parallel or criss-crossing each other may be designed on the outer casing 20 to increase the strength of the outer casing.
- the outer casing 20 can also be subjected to painting, computer printing, computer engraving, etching or electroplating to make the outer casing have a better appearance.
- the outer casing 20 is provided with an instrument panel 22 and a door 24 which can be opened and closed a plurality of times.
- the instrument panel 22 can be designed with a display screen and a plurality of operation keys to facilitate the operation of the operator.
- the instrument panel 22 is designed with a touch liquid crystal display, so that the operator can directly touch the liquid crystal display to operate.
- the instrument body 30 includes a bottom plate 32 fixed to the base 10, an inner casing 34, a gantry 36, a console 38, a power pack 40, a main control unit 42, and a first motor unit 44, The second motor group 46 and the third motor group 48.
- the bottom plate 32 provides at least one plane for supporting the aforementioned components.
- the bottom plate 32 is a steel plate having a thickness of 1.0 to 10.0 mm, which should have moderate rigidity and can maintain at least one face in a planar shape, which is less prone to bending, twisting, and local lifting.
- the bottom plate 32 can also be made by a hard plastic injection molding process, or a hard wood or bamboo material can be produced by a woodworking process.
- the bottom plate 32 can also serve as the base 10 when the bottom plate 32 has sufficient thickness, rigidity, and mass.
- the inner casing 34, the gantry 36, the console 38, the power pack 40 and the first motor unit 44 are respectively fixed to the bottom plate 32.
- the main control unit 42 is placed on the back of the power pack 40 (shown in Figure 3). As shown in FIGS.
- the first motor unit 44 is fixed to the upper surface of the bottom plate 32, and its longitudinal direction or axis is parallel to the upper surface of the bottom plate; the second motor group 46 and the third motor group 48 are first. It is fixed to the motor bracket 50 and then mounted to the first motor unit 44 by the motor bracket 50.
- the second motor group 46 and the third motor group 48 are arranged adjacent to each other in parallel with their axes parallel to each other and perpendicular to the upper surface of the bottom plate 32, respectively.
- the gantry 36 is provided with a guide rail or a slide rail 43.
- the motor bracket 50 is provided with a slider 51 corresponding to the guide rail 43, and the slider 51 is defined to slide within the guide rail 43.
- the gantry 36 includes at least one beam 37 and two vertical beams 39 and 41 for supporting the girders 37.
- the guide rails 43 are designed on the beam 37, and the vertical beams 39 and 41 are respectively fixed to the bottom plate 32 or the upper surface of the base 10.
- the console 38 includes a flat, generally rectangular parallelepiped operation panel 52 adjacent the front side of the nucleic acid extractor 1.
- the operation panel 52 should have appropriate rigidity and strength and is not easily deformed.
- the operation panel 52 may be fabricated by a cold working process from a stainless steel plate or by an injection molding process of a hard plastic.
- the operation panel 52 includes a The platform for extracting nucleic acids is substantially completed on the platform, which is substantially parallel to the upper surface of the bottom plate 32.
- the platform includes two longitudinal sides that are substantially parallel to the front side of the nucleic acid extractor 1.
- the first motor unit 44 is mounted at the rear of the console 38 (i.e., at a position further away from the front of the nucleic acid extractor than the console 38, see Figs. 6 and 7).
- the longitudinal direction or the axial direction of the first motor group 44 is substantially parallel to the longitudinal direction side of the console 38.
- Power pack 40 includes an elementary component (not labeled) that converts a 220 volt or 110 volt universal voltage into a voltage suitable for driving various electronic components within the nucleic acid extractor of the present invention and at least one set of power management components 54. To save space, the power management component 54 is secured above the power pack 40 (as shown in Figures 4 and 5). Referring to FIG. 24 and FIG. 24A, the power management component 54 includes at least one printed circuit board 56, a plurality of electronic components such as capacitors and resistors, and at least one power conditioner 58 and heat sink 60. The heat sink 60 abuts the power conditioner 58.
- the power conditioner 58 includes heat-generating electronic components for consuming the instantaneous overload voltage or overload current that may be generated during operation of the nucleic acid extractor into thermal energy.
- the heat sink 60 is used to conduct the heat generated by the power conditioner 58 in time to cool the power conditioner 58 to avoid burning the power conditioner 58 due to excessive temperature.
- the motor bracket 50 includes a first panel 62 and a second panel 64 that are substantially perpendicular to each other, wherein the second panel 62 is fixed with a second motor group 46 and a third motor group 48, and the second panel 64 is Fixed to the first motor group 44.
- the motor bracket 50 can be made by a cold working process of a steel plate or by an injection molding process of a hard plastic. Motor The bracket 50 is generally in the shape of a capital letter "L" as a whole. To increase the strength of the motor bracket 50, and to save material and reduce weight, at least one weir or rib 66 is provided at the angle between the first panel 62 and the second panel 64 at a substantially 90 degree angle. As shown in FIGS.
- the first motor unit 44 includes a motor 68 that is fixed to the upper surface of the bottom plate 32 and that does not have a relative displacement with respect to the bottom plate 32, and is coaxially connected to the shaft of the motor 68 by the connector 70.
- the shaft of the motor 68 drives the ball screw 72 to rotate along its axis through the connector.
- the axis rotation of the ball screw 72 drives the slider 74 to reciprocate along the axis of the ball screw.
- the slider 74 drives the motor bracket 50 when the slider 74 reciprocates along the axis of the ball screw 72.
- the second and third motor units 46, 48 reciprocate between positions "A" and "B” in a plane parallel to the bottom plate 32 and the longitudinal direction of the console 38 (as shown in Fig. 25).
- the nucleic acid extractor of the present invention includes a magnetic rod holder 78 fixedly coupled to the slider of the second motor unit 46 and a magnetic rod holder 80 fixedly coupled to the slider of the third motor unit 48.
- the magnetic bar holder 78 is provided with a plurality of rows of magnetic bars 82 downwardly, each row having a plurality of magnetic bars (preferably 4 x 8 magnetic bars).
- the magnetic rod holder 80 is provided with a number of channels 84 corresponding to the number of magnetic rod rows (e.g., four). Each of the channels 84 can be inserted into a magnetic rod sleeve.
- the nucleic acid extractor 1 of the present invention illustrates the operation of the nucleic acid extractor 1 of the present invention.
- the nucleic acid extractor 1 of the present invention is sequentially operated in accordance with the following steps.
- the first step loading - put the deep well plate (also called 96-well plate) 88 after the sample is added to the console 38
- the magnetic rod sleeve 86 is then secured to the channel 84 of the magnet holder 80.
- the second step, cracking starts the third motor unit 48, causing the magnetic rod sleeve 80 to drive the magnetic rod sleeve 86 down until the magnetic rod sleeve 86 enters the hole of the designated row of the deep hole plate 88, and then the third motor unit 48 drives the magnetic rod sleeve 80 and the magnetic rod sleeve 86 together for a predetermined time at a frequency selected from (2-100) HZ (preferably 2, 4, 8, 16, 21, 30, 50, 80, 100 Hz), magnetic
- the end of the rod sleeve 86 remote from the rod sleeve 80 is twisted in the hole of the deep orifice plate 88 with a mixture of the sample, the lysate and the magnetic beads (which have been previously placed in the holes of the deep well plate) so that the sample is in the sample.
- the nucleic acid is separated from the sample (ie, the nucleic acid is cleaved) and adsorbed onto the magnetic beads.
- the third step, adsorption - the second motor unit 46 starts and drives the magnetic rod holder 78 down until the magnetic rod 82 enters the designated position of the magnetic rod sleeve 86, and is allowed to stand for a predetermined time, so that all the magnetic beads are separated from the sample.
- the nucleic acid is adsorbed together on the outer surface of the magnetic rod sleeve 86, and then the magnetic rod holder 78 and the magnetic rod sleeve 86 are moved upward together to cause the magnetic rod sleeve 86 and the magnetic rod 82 to enter the washing holes of the designated row of the deep well plate 88 together. Wash to remove impurities.
- the elution-magnetic bar holder 78 and the magnetic rod sleeve 86 are brought into the elution hole from the washing hole, and the nucleic acid is released from the magnetic beads by the eluent.
- the magnetic beads are recovered - the magnetic beads adsorbed on the outer surface of the magnetic rod sleeve 86 are transferred from the elution holes into the magnetic bead recovery holes, and the magnetic rods 82 are separated from the magnetic rod sleeve 86.
- the magnetic detachment - the magnetic rod sleeve 86 vibrates in the magnetic bead recovery hole, so that the magnetic beads are all detached from the outer surface of the magnetic rod sleeve 86.
- Step 7 clean up - remove the magnetic rod sleeve 86, remove the deep well plate, and return the instrument to the starting position. Among them, in the cleavage and elution steps, it is necessary to heat the sample mixture in the deep well plate to a preset temperature range.
- the first, second and third motor groups 44, 46, 48 have substantially the same structure, and both adopt precise stepping stepping motors and ball screws.
- the repeatability of the stepper motor used ie, the displacement error produced by each cycle
- the first motor unit 44 functions to drive the second and third motor groups 46, 48 to move between the first position and the second position in accordance with a predetermined program.
- the second motor unit 46 functions to drive the magnetic bar frame 78 up and down.
- the function of the third motor unit 48 is to drive the magnetic rod sleeve 80 up and down and to vibrate.
- the present invention provides an encoder 90 on the third motor unit 48.
- the encoder 90 includes components for monitoring the operation of the stepper motor and components for issuing correction commands to the stepper motor.
- the encoder issues a correction command to correct the output of the stepper motor according to each pulse.
- the stroke thus avoids the occurrence of motor out-of-step in advance, and improves the accuracy of the vibration of the third motor unit 48 driving the magnetic rod sleeve 80.
- the technical solution adopted by the present invention is to fix a light sensing arm 92 on the outer side of the slider 74 of each motor group, and to provide an optocoupler sensor 93 in the initial position and the maximum stroke position of the sliding 74. .
- the optocoupler sensor 93 monitors the position of the slider 74 by sensing the position of the light sensing arm 92.
- the first motor group 44 can accurately control the second and third motor groups (that is, The magnetic rod holder 80 and the magnetic rod holder 78) correspond to the position of the deep hole plate 88 for cracking, washing, eluting and recovering the magnetic beads; the second and third motor units can accurately control the magnetic rod holder 80 and the magnetic rod holder The lowest and highest position of 78.
- each row of heater plates 94 includes a plurality of heating orifice walls 96 that closely conform to the shape of the bottom of the deep orifice plate 88.
- the heating plate 94 is preferably made of a metal material, and has a heating member (not shown) such as a resistance wire inserted therein, and a heat insulating member (not labeled) is provided on the outer periphery thereof.
- a plurality of mutually independent compression springs 98 are disposed under the heating plate 94 to urge all of the heating hole walls 96 of the heating plate 94 to more evenly adhere to the bottom of the deep hole plate 88, thereby making the heat of the respective holes more uniform. This will greatly improve the cleavage and elution efficiency of the samples in each well on the deep well plate 88, thereby greatly reducing the error in nucleic acid extraction efficiency in each well.
- the console 38 includes at least one set of clamps for automatically grasping and loosening the deep orifice plate 88 placed on the console 38.
- the jig includes a motor 100 located below the bottom plate 32 (i.e., the back of the console 38) (the number of motors corresponds to the number of deep-hole plates), a support portion 104 located inside the console 38, a plurality of clamp arms 106, and micro Switch 108.
- the clamp arm 106 includes a shaft 105. The up and down movement of the support portion pushes the clamp arm to rotate about the shaft, and the clamp arm 107 clamps or loosens the deep orifice plate.
- Motor 100 includes a cam 102.
- the cam 102 pushes the support portion 104 up and down.
- the support portion 104 further urges the clamp arm 106 to rotate clockwise or counterclockwise in accordance with the principle of leverage, and the clamp arm 106 clamps or releases the outer wall of the deep orifice plate 88.
- a torsion spring (not numbered) is disposed on the clamp arm 106.
- the torsion spring keeps the clamp arm in a released state to reduce the placement of the deep hole plate 88 to the console 38. Obstruction.
- the microswitch 108 is located near one end of the console 38.
- the microswitch 108 includes a spring leaf 110 and a push button switch 112.
- Fig. 18A is a state in which the micro switch is in a closed state
- Fig. 19A is a state in which the micro switch is in an on state.
- the support portion 104 further urges the clamp arm 106 to rotate in accordance with the principle of the lever, so that the clamp arm 106 grips the outer wall of the deep orifice plate 88, thereby precisely fixing the position of the deep orifice plate 88 on the console 38.
- the operator issues an instruction to release the deep well plate 88 through the instrument panel 22.
- the motor 100 drives the cam 102 to operate, and the support portion 104 moves downward under the action of the spring and its own gravity.
- the clamp arm 106 Under the action of the torsion spring, the clamp arm 106 is reversed, returning to the state in which the deep hole plate 88 is released, and no clamping force is applied to the deep hole plate. Thus, the operator can easily remove the deep well plate 88 from the console 38.
- the console 38 is provided at its end adjacent the instrument panel 22 with a cross flow fan 114 for dissipating heat outside the instrument.
- the cross flow fan 114 is hidden below the operation panel 52 of the console 38.
- the air outlet of the cross flow fan 114 is located at the bottom of the nucleic acid extractor of the present invention.
- the instrument body 30 of the nucleic acid extractor of the present invention is substantially enclosed by the base 10 and the outer casing 20, and an air outlet is provided for the cross flow fan 114 only at the bottom of the base 10.
- the nucleic acid extractor of the invention is further provided with an inner casing 34 and an operation Panel 52.
- the inner casing 34 and the operating panel 52 substantially confine the deep orifice plate 88 to a relatively enclosed space.
- the door 24 of the nucleic acid extractor 1 of the present invention includes a door handle 116 and a door shaft 118.
- the outer casing 20 is provided with a permanent magnet magnet block (not shown) at a position corresponding to the door handle 116.
- the permanent magnet magnet block sucks the door handle 116, thereby keeping the door 24 closed.
- the door shaft 118 includes a door inner shaft 120 fixed to the base 10 and a door bushing 122 fixed to the bottom two ends of the door.
- the two ends of the door center shaft 120 are respectively incorporated into the door bushing 122, and a door shaft torsion spring 124 is provided at the joint of the door center shaft 120 and the door bushing 122.
- the door shaft torsion spring 124 maintains a certain damping coefficient for the combination between the door center shaft 120 and the door bushing 122, thereby increasing the hand feeling when the door 24 is opened and closed, which increases the operator's pleasure.
- Figure 34 illustrates the working principle and steps of the nucleic acid extractor of the present invention.
- Step 1 Add a sample (such as whole blood) to the deep well plate.
- Step 2 Heat and lyse the sample under the on-board vibration of the magnetic rod sleeve.
- the third step the magnetic rod enters the magnetic rod sleeve and adsorbs the magnetic beads and the nucleic acid.
- the fourth step washing off the impurities under the action of the washing liquid.
- Step 5 The nucleic acid is eluted from the magnetic rod sleeve under the action of the eluent.
- Step 6 Recycle the magnetic beads.
- Step 7 The magnetic rod is removed from the magnetic rod sleeve, and under the mechanical vibration of the magnetic rod sleeve, the magnetic beads are detached from the magnetic rod sleeve and fall into the magnetic bead recovery hole.
- Figure 35 is a diagram showing the step of fixing the deep well plate of the nucleic acid extractor of the present invention and heating the sample.
- Step 1 Place the deep well plate.
- Step 2 Trigger the micro switch.
- the third step CPU signal response.
- the fourth step the motor starts.
- Step 5 The support is moved up.
- First Six steps clamping arm clamping.
- Step 7 The heating plate is closely attached to the deep hole plate.
- a part A of the present patent application (including the patent application specification and the claims) is fixed to another part B or a similar description means that a part A is directly or indirectly fixed to another part B, including One component A and the other component B are fixedly coupled to each other in contact with each other, and a component A and another component B are not in contact with each other, but are fixedly connected by a third component.
- a part A is directly or indirectly fixed to another part B, including One component A and the other component B are fixedly coupled to each other in contact with each other, and a component A and another component B are not in contact with each other, but are fixedly connected by a third component.
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Abstract
Description
Claims (10)
- 一种对样本进行加热的方法,其特征在于利用核酸提取仪按照设定的步骤进行加热,其中所述核酸提取仪包括:基座、与基座相连的外机壳、位于外机壳的内部并且被安装到基座的仪器主体,仪器主体包括电源组、主控装置和直流电机,所述设定的步骤包括:第一步,把样本加入到深孔板;第二步,把深孔板放置到所述核酸提取仪内的指定位置;第三步,触发核酸提取仪上的微动开关;第四步,启动核酸提取仪的主控装置;第五步,启动直流电机;第六步,核酸提取仪的支撑部上移;第七步,核酸提取仪的夹臂夹紧深孔板;第八步,加热板与深孔板紧密贴合;第九步,加热装置启动加热模式。
- 如权利要求1所述的加热方法,其特征在于:在把深孔板放置到所述核酸提取仪内指定位置时,深孔板自动触发核酸提取仪上的微动开关。
- 如权利要求2所述的加热方法,其特征在于:所述微动开关包括一个倾斜的弹簧片和一个按钮开关,当深孔板被放置到操作台的预定位置时,深孔板的底部推动弹簧片压缩按钮开关,从而触发微动开关,启动电机工作。
- 如权利要求1所述的加热方法,其特征在于:采用了一种用于固定深孔板的夹具,该夹具包括位于核酸提取仪之底板下方的电机、位于核酸提取仪之操作台内部的支撑部、若干个夹臂和微动开关,其中,电机包括一个凸轮;在电机启动时,凸轮推动支撑部上、下移动;支撑部进一步推动夹臂转动,夹臂夹紧或者释放深孔板的外壁;在夹臂上设置有扭簧,当操作台没有放置深孔板时,扭簧使夹臂保持松开状 态。
- 如权利要求1-4之任一者所述的加热方法,其特征在于:在加热温度和时间达到预先设置的要求后还包括冷却步骤,所述冷却步骤包括采用安置在核酸提取仪之操作台下方的散热装置进行散热。
- 如权利要求5所述的加热方法,其特征在于:所述散热装置包括被隐藏在操作台下方的横流风机。
- 如权利要求1-4之任一者所述的加热方法,其特征在于:所述加热装置包括设置在核酸提取仪之操作台上的若干排活动的加热板,每一排加热板包括与被加热的深孔板之底部形状密切吻合的若干个加热孔壁,加热板采用导热材料,其内部贯穿有加热部件,其外周设置有隔热部件。
- 如权利要求7所述的加热方法,其特征在于:在加热板的下面设置有若干个相互独立的压缩弹簧,压缩弹簧抵靠在加热板的下方。
- 如权利要求8所述的加热方法,其特征在于:加热板的所有加热孔壁贴附深孔板的底部。
- 如权利要求9所述的加热方法,其特征在于:所述加热部件选自于电阻、电阻丝、电容、IC集成电路、三极管、陶瓷。
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