WO2014136776A1 - 蛍光標識用樹脂粒子 - Google Patents
蛍光標識用樹脂粒子 Download PDFInfo
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- WO2014136776A1 WO2014136776A1 PCT/JP2014/055469 JP2014055469W WO2014136776A1 WO 2014136776 A1 WO2014136776 A1 WO 2014136776A1 JP 2014055469 W JP2014055469 W JP 2014055469W WO 2014136776 A1 WO2014136776 A1 WO 2014136776A1
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- fluorescent
- resin
- fluorescent dye
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- dye
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Images
Classifications
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- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
- G01N33/533—Production of labelled immunochemicals with fluorescent label
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
- C08L101/00—Compositions of unspecified macromolecular compounds
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
- C09B67/00—Influencing the physical, e.g. the dyeing or printing properties of dyestuffs without chemical reactions, e.g. by treating with solvents grinding or grinding assistants, coating of pigments or dyes; Process features in the making of dyestuff preparations; Dyestuff preparations of a special physical nature, e.g. tablets, films
- C09B67/0001—Post-treatment of organic pigments or dyes
- C09B67/0004—Coated particulate pigments or dyes
- C09B67/0008—Coated particulate pigments or dyes with organic coatings
- C09B67/0009—Coated particulate pigments or dyes with organic coatings containing organic acid derivatives
- C09B67/001—Coated particulate pigments or dyes with organic coatings containing organic acid derivatives containing resinic acid derivatives
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
- C09B67/00—Influencing the physical, e.g. the dyeing or printing properties of dyestuffs without chemical reactions, e.g. by treating with solvents grinding or grinding assistants, coating of pigments or dyes; Process features in the making of dyestuff preparations; Dyestuff preparations of a special physical nature, e.g. tablets, films
- C09B67/0001—Post-treatment of organic pigments or dyes
- C09B67/0017—Influencing the physical properties by treatment with an acid, H2SO4
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- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
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- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
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- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
- G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
- G01N2021/6441—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks with two or more labels
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- G01N2800/7023—(Hyper)proliferation
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Definitions
- the present invention relates to a resin particle for fluorescent labeling that can be used in pathological diagnosis and the like.
- Pathological diagnosis is performed as one of medical diagnoses.
- the pathologist diagnoses the disease from a piece of tissue collected from the human body and tells the clinician whether treatment or surgery is necessary.
- the medical doctor determines the drug treatment policy, and the surgical doctor determines whether or not to perform the operation.
- tissue specimens obtained by organ excision and needle biopsy are sliced to a thickness of several microns to create tissue specimens, and they are widely observed with an optical microscope to obtain various findings. It has been broken.
- a specimen is prepared by dehydrating and collecting a paraffin block to fix the collected tissue, then slicing it to a thickness of several ⁇ m, and removing the paraffin.
- DAB staining is a dye staining method using peroxidase with diaminobenzidine (DAB) as a substrate as an enzyme label.
- staining with an enzyme label such as DAB staining has a problem that it is difficult to estimate the actual antibody expression level from the staining concentration, because the staining concentration greatly depends on environmental conditions such as temperature and time. For this reason, in immunological observation in pathological diagnosis, a fluorescent labeling method using a fluorescent label excellent in quantitative performance instead of enzyme labeling has recently been used in pathological diagnosis.
- Patent Document 1 discloses fluorescent particles containing one or more organic dyes and an organic fluorescent dye (FRET donor dye and acceptor dye) having an excitation wavelength longer than that of the organic dye.
- FRET donor dye and acceptor dye organic fluorescent dye
- Patent Document 2 describes fluorescent organic dye-encapsulated silica nanoparticles containing a fluorescent organic dye (Texas Red is used in the examples) used in a pathological diagnosis information generation method (system).
- Patent Document 3 discloses one or more hydrophilic organic dyes or organic fluorescent dyes (in the examples, 8-hydroxy-1,3,6-pyrenetrisulfonic acid sodium salt is used). Melamine-formaldehyde particles containing are described.
- the phosphor is likely to fade due to the excitation light collected by the lens. There is a problem that it is difficult.
- a significant decrease in emission intensity due to discoloration causes a decrease in the number of bright spots and deteriorates quantitativeness.
- the phosphors based on silica as described in Patent Documents 1 and 2 generally have low fluorescence intensity and low light resistance.
- fluorescent dye-containing particles based on melamine / formaldehyde (melamine resin) using organic fluorescent dyes specifically described in Patent Document 3 have room for improvement in fluorescence intensity and light resistance.
- 8-hydroxy-1,3,6-pyrenetrisulfonic acid sodium salt used in Examples of Patent Document 3 is not a fluorescent dye that satisfies the specific criteria of the present invention described later.
- the present inventors select a fluorescent dye that can solve the above-mentioned problems by selecting a fluorescent dye to be immobilized on the resin particle that satisfies a specific standard related to the concentration of the fluorescent dye aqueous solution and its emission intensity.
- the present invention was completed. That is, a fluorescent dye that does not easily cause concentration quenching is selected according to a specific standard, and typically, the resin synthesis reaction proceeds while mixing with the resin raw material, and the fluorescent dye is encapsulated in the formed resin particles.
- the resin particles for fluorescent labeling obtained by this method do not cause concentration quenching while fixing (encapsulating) a relatively large amount of fluorescent dye, so that they are difficult to fade even if the fluorescence is applied for a certain period of time during fluorescence observation. .
- [Claim 1] A fluorescent dye resin particle, wherein a fluorescent dye satisfying both of the following conditions (1) and (2) is immobilized on a resin particle.
- the peak of the emission intensity depending on the concentration of the fluorescent dye alone in the aqueous solution is in the range of 30 to 80 ⁇ M (M: molar concentration mol / liter).
- M molar concentration mol / liter
- the emission intensity at a concentration of 100 ⁇ M is 80% or more of the peak emission intensity.
- [Section 2] Item 2.
- the fluorescent labeling resin particle according to Item 1, wherein the Stokes shift in the aqueous solution of the fluorescent dye is 25 nm or more.
- Luminance Molar extinction coefficient ⁇ Quantum yield ⁇ 1/1000
- the resin particles for fluorescent labeling of the present invention are superior in light resistance and difficult to fade compared to conventional particles, it is possible to suppress a decrease in fluorescence intensity with the passage of observation (excitation light irradiation) time. Moreover, the resin particle for fluorescent labeling of the present invention satisfies a high level in terms of initial emission intensity. As a result, bright spot measurement can be performed while maintaining quantitativeness without being affected by the observation time. Such an effect is particularly useful for improving the accuracy of pathological diagnosis.
- FIG. 1 is a graph showing the relationship between the concentration of an aqueous solution and the emission intensity when the five typical fluorescent dyes used in Examples and Comparative Examples are not subjected to water solubilization treatment.
- the fluorescent labeling resin particle is a term that collectively refers to substances having a structure in which a plurality of fluorescent dye molecules are immobilized on the resin particle by a chemical or physical action, and the form thereof is not particularly limited.
- encapsulated type in which the fluorescent dye is mainly encapsulated in the resin particles, which is obtained by a production method in which a polymerization reaction of the resin proceeds while mixing a resin raw material such as melamine resin and the fluorescent dye.
- fluorescent labeling resin particles examples include fluorescent labeling resin particles.
- Such encapsulated fluorescent labeling resin particles can immobilize a large number of fluorescent dye molecules, but by selecting an appropriate fluorescent dye according to the present invention, concentration quenching can be suppressed and high fluorescence intensity and light resistance can be achieved. Can be achieved.
- resin particles for fluorescent labeling in which a fluorescent dye is encapsulated in resin particles for example, resin particles made of melamine resin) containing a thermosetting resin in which a dense cross-linked structure is formed are preferable.
- the fluorescent dye is mainly adsorbed on the surface of the resin particles obtained by a manufacturing method of adding the fluorescent dye to the dispersion.
- the fluorescent labeling resin particles in the form (adsorption type) are also included.
- the fluorescent dye is confined in the particle by at least the molecular structure of the resin (for example, a three-dimensional network structure possessed by a thermosetting resin such as melamine resin). Furthermore, an electrostatic interaction (ionic bond) works between the functional group or part of the fluorescent dye and the functional group or part of the resin, or a covalent bond is formed between them. It is preferable that the fluorescent dye is more firmly fixed to the resin particles since the fluorescent dye can be prevented from being eluted out of the particles by the organic solvent (xylene) used in the tissue staining penetration process.
- the organic solvent xylene
- the above “electrostatic interaction” works when the fluorescent dye has a positively or negatively charged substituent or site, and the resin molecule has an oppositely charged substituent or site.
- having a positively or negatively charged substituent or moiety only needs to be in such a state when the fluorescent dye or resin is dissolved in neutral, acidic or basic water.
- the negatively charged substituent or moiety is typically a sulfo group (—SO 3 ⁇ ), but other examples include a phosphoryl group, a phenolic hydroxyl group, and a carboxyl group.
- the positively charged substituent or moiety is typically an amino group (—NH 3 + ), but in addition, an aromatic amino group, a cyclic amino group (pyridinyl group, triazolyl group, etc.), a hydrazinyl group, etc. Is mentioned.
- Substituents or sites as described above for the resin may be those introduced into the fluorescent dye and the resin raw material (monomer) prior to the production process of the resin particle for fluorescent labeling, for example, melamine resin, An amino group contained in a urea resin or the like may be formed in a process in which H + is attached to the amino group and protonated.
- substituents or sites for the fluorescent dye may be those previously possessed by a fluorescent dye molecule that is commercially available as a commercial product, or “water-soluble treatment” ( It may be introduced by acid treatment). Since the electrostatic interaction tends to become stronger as the number of charged substituents and the like possessed by the resin and the fluorescent dye (per molecule or per molecular weight of a predetermined unit) increases, the electrostatic interaction of the desired strength It is preferable to adjust their number so that the action works.
- examples of the “covalent bond” include an amide bond, an ester bond, an ether bond, and a CN bond.
- a fluorescent dye and a resin raw material (monomer) having a functional group or a site capable of forming a covalent bond as described above are selected, and a pre-reaction is performed in advance prior to the resin synthesis reaction. What is necessary is just to make the obtained derivatized resin raw material react in a superposition
- the unreacted product reacts with the resin raw material or the produced resin to form a covalent bond with the fluorescent dye added to the resin raw material in the polymerization process.
- a style may be used.
- the fluorescent labeling resin particles are of the adsorption type
- the functional group or site on the surface of the resin particle and the functional group or site of the fluorescent dye have There must be an electrostatic interaction between them, or a covalent bond must be formed between them.
- the same electrostatic interaction as in the case of the inclusion type described above can be used.
- the process for forming the covalent bond similar to the case of the inclusion type mentioned above may be provided after the synthesis of the resin particles.
- the particle diameter of the fluorescent labeling resin particles is not particularly limited as long as it is a particle diameter suitable for an application such as immunostaining of a tissue section, but is usually 10 to 500 nm, and preferably 50 to 200 nm. Further, the coefficient of variation indicating the variation in particle diameter is not particularly limited, but is usually 20% or less, and preferably 5 to 15%.
- the fluorescent labeling resin particles having such a particle diameter can be obtained, for example, by a manufacturing method as described later.
- the particle diameter of the fluorescent labeling resin particles is obtained by taking an electron micrograph using a scanning electron microscope (SEM), measuring the cross-sectional area of the fluorescent labeling resin particles, and measuring the measured value with the corresponding circle area. Can be measured as the diameter (area circle equivalent diameter).
- the average (average particle size) and coefficient of variation of the particle size of the fluorescent labeling resin particle population are measured as described above for a sufficient number (for example, 1000) of fluorescent labeling resin particles. After that, the average particle diameter is calculated as its arithmetic average, and the coefficient of variation is calculated by the formula: 100 ⁇ standard deviation of particle diameter / average particle diameter.
- the fluorescent labeling resin particles of the present invention have a sufficient initial emission intensity at the start of excitation light irradiation, and can maintain a constant emission intensity even after irradiation with excitation light for a predetermined time. It can be manufactured as an excellent one.
- the initial emission intensity and the retention rate of the emission intensity vary depending on various conditions and materials selected for the production of the fluorescent labeling resin particles, and the evaluation method, and an absolute evaluation standard cannot be established. .
- the fluorescent labeling resin particles of the present invention are preferably Has a retention of emission intensity of 60% or more, more preferably 75% or more.
- the fluorescent dye in the present invention has (1) the peak of the emission intensity depending on the concentration of the fluorescent dye alone in the aqueous solution is in the range of 30 to 80 ⁇ M, and (2) the emission intensity at the concentration of 100 ⁇ M is the peak. There is no particular limitation as long as both the conditions of 80% or more of the emission intensity are satisfied.
- Whether or not the condition (1) is satisfied indicates a peak of the emission intensity when a multi-stage dilution of an aqueous solution of a fluorescent dye alone is prepared and the emission intensity of each dilution is measured and compared under the same conditions. Whether the concentration of the diluted solution is within the range of 30 to 80 ⁇ M can be determined.
- Whether or not the condition (2) is satisfied is determined by preparing a diluted solution having a concentration of 100 ⁇ M at the time of measurement related to the above condition (1), and whether or not the emission intensity is 80% or more of the emission intensity of the peak. Can be determined.
- the condition (2) is preferably such that the emission intensity at a concentration of 100 ⁇ M is 85% or more of the peak emission intensity, more preferably 90% or more.
- the method for measuring the emission intensity is not particularly limited, and it may be measured under general measurement conditions using a general fluorometer.
- the wavelength of the excitation light to be irradiated and the emission wavelength to be measured are respectively set to the maximum excitation wavelength and the maximum emission wavelength for the fluorescent dye, or a wavelength in the vicinity thereof.
- the slit widths for excitation light and light emission may be adjusted within an appropriate range.
- the maximum excitation wavelength and the maximum emission wavelength can be measured by a general method. If the fluorescent dye is a commercial product, it may be regarded as the wavelength described in the catalog.
- the larger the Stokes shift of the fluorescent dye that is, the difference between the maximum excitation wavelength and the maximum emission wavelength, the smaller the overlap between the absorption spectrum and the emission spectrum (self-absorption).
- concentration quenching is suppressed, and resin particles for fluorescent labeling with high emission intensity can be easily obtained, and quantitative sensitivity is also improved.
- the magnitude of the Stokes shift of the fluorescent dye used is not particularly limited.
- a fluorescent dye having a Stokes shift of 25 nm or more It is preferable because it is easy to satisfy (1) and (2) and it is easy to produce fluorescent labeling resin particles having appropriate fluorescence intensity according to the application.
- Fluorescent dyes can easily satisfy the conditions (1) and (2) when subjected to water-solubilization treatment. That is, among fluorescent dyes that do not normally satisfy the conditions (1) and (2), the conditions (1) and (2) are satisfied by performing the water-solubilization treatment, and the conditions ( Similar to the fluorescent dyes that satisfy 1) and (2), there are those that become fluorescent dyes that can be used in the present invention.
- the fluorescent dye that satisfies the conditions (1) and (2) without being subjected to the water-solubilization treatment is also the one after the water-solubilization treatment (usually, the fact that the conditions (1) and (2) are naturally satisfied is maintained). It may be used.
- the water-solubilization treatment of fluorescent dyes improves the dispersity and emission intensity of dyes, especially in encapsulated fluorescent labeling resin particles. As a result, particles with higher emission intensity and higher light resistance can be obtained, and quantification in pathological observation Sensitivity can be improved.
- the Stokes shift of the fluorescent dye may spread to 25 nm or more by performing the water-solubilization treatment.
- a fluorescent dye having a Stokes shift of less than 25 nm may be used with a Stokes shift of 25 nm or more due to the water-solubilization treatment.
- the water-solubilization treatment is not particularly limited as long as it is a technique that can solubilize the fluorescent dye, that is, improve the solubility in water.
- Specific examples of the water solubilization treatment include a method in which a fluorescent dye is treated with an acid (concentrated sulfuric acid, concentrated hydrochloric acid, acetic acid, formic acid, etc.) or an aldehyde (formaldehyde, acetaldehyde, etc.), and these are reacted with the fluorescent dye.
- acid treatment which is generally excellent in effect is preferred.
- the compound used for the water-solubilization treatment for example, the preferred acid used for the acid treatment varies depending on the fluorescent dye to be treated.
- concentrated hydrochloric acid is suitable for HiLyte Fluor, and when concentrated sulfuric acid is used, it is quenched.
- Concentrated sulfuric acid is suitable for Dylight 594, and when concentrated hydrochloric acid is used, it will fade.
- those skilled in the art can select an appropriate one from candidate compounds (acids) without undue trial and error.
- the measured value of the luminescence intensity can be a relative numerical value expressed in arbitrary units au (arbitrary unit) as long as the measurement conditions of the comparison objects match.
- the measured value of the luminescence intensity can be a relative numerical value expressed in arbitrary units au (arbitrary unit) as long as the measurement conditions of the comparison objects match.
- luminance represented by the following formula.
- Luminance Molar extinction coefficient ( ⁇ ) ⁇ Quantum yield ( ⁇ ) ⁇ 1/1000
- the brightness represented by the above formula of the fluorescent dye used in the present invention is not particularly limited as long as it can produce fluorescent labeling resin particles having appropriate fluorescence intensity according to the application.
- a fluorescent dye having a luminance at the peak of emission intensity as described above of 100,000 or more is preferable because it is easy to produce such fluorescent labeling resin particles.
- Fluorescent dyes that can be generally obtained or produced include, for example, rhodamine dye molecules, squarylium dye molecules, cyanine dye molecules, aromatic hydrocarbon dye molecules, oxazine dye molecules, carbopyronine dye molecules, and pyromesene dyes.
- Dye molecules Alexa Fluor (registered trademark, manufactured by Invitrogen) dye molecule, BODIPY (registered trademark, manufactured by Invitrogen) dye molecule, Cy (registered trademark, manufactured by GE Healthcare) dye molecule, DY dye Molecules (registered trademark, manufactured by DYOMICICS), HiLyte (registered trademark, manufactured by Anaspec) dye molecule, DyLight (registered trademark, manufactured by Thermo Scientific) dye molecule, ATTO (registered trademark, manufactured by ATTO-TEC) ) Dye molecules, MFP (registered trademark, manufactured by Mobitec) It can be classified into molecular iodine.
- the generic names of such dye molecules are named based on the main structure (skeleton) in the compound or the registered trademark, and the range of fluorescent dyes belonging to each is appropriate for those skilled in the art without undue trial and error. Can be grasped.
- rhodamine dye molecules include 5-carboxy-rhodamine, 6-carboxy-rhodamine, 5,6-dicarboxy-rhodamine, rhodamine 6G, tetramethylrhodamine, X-rhodamine (ROX), 5-carboxy-X. -Rhodamine (5-ROX), Texas Red (Texas Red), Spectrum Red, LD700 PERCHLORATE, CAL Fluor Red 610, CAL Fluor Red 615, and the like.
- squarylium dye molecule examples include SRfluor 680-Carboxylate, 1,3-Bis [4- (dimethylamino) -2-hydroxyphenyl] -2,4-dihydroxycyclobutenedidium dihydroxide, bis, 1,3-Bis, dimethylyl)) phenyl] -2,4-dihydroxycyclobutenedidium dihydroxide, bis, 2- (4- (Dithylamino) -2-hydroxy-4-hydroxy, 4- (dithyrimino) -2-hydryloxy-2-hydroxy-3-2-hydroxy-3 oxocyclobut-1-eno ate, 2- (4- (Dibutylamino) -2-hydroxyphenyl) -4- (4- (dibutylimino) -2-hydroxycyclohexa-2,5-diylenedene) -3-oxycyclobut-1-enolate, 2- (8-Hydroxy) -1,1,7,7-tetramethyl
- cyanine dye molecules include 1-Butyl-2- [5- (1-butyl-1,3-dihydro-3,3-dimethyl-2H-indol-2-ylidene) -penta-1,3. -Dienyl] -3,3-dimethyl-3eiti-indolium hexafluorophosphate, 1-Butyl-2- [5- (1-butyl-3,3-dimethyl-1,3-dihydro-indol-2-ylidene) -3- chloro-penta-1,3-dienyl] -3,3-dimethyl-3H-indolium hexafluorophosphate, 3-Ethyl-2- [5- (3-ethyl-3H-benzothiazol-2-ylide e) -penta-1,3-dienyl] -benzothiazol-3-ium iodide, and the like.
- aromatic hydrocarbon dye molecule examples include N, N-Bis- (2,6-diisopropylphenyl) -1,6,7,12- (4-tert-butylphenoxy) -perylene-3,4,9 , 10-tetracarbonbonide diimide, N, N′-Bis (2,6-diisopropenylphenyl) -1,6,7,12-tetraphenylperylene-3, 4: 9,10-tetracarboximide, N, N′-Bis (2,6 -Diisopropylphenyl) perylene-3,4,9,10-bis (dicarbide), 16, N, N'-Bis (2,6-dimethylphenyl) perylene-3,4,9,10-tetracar boxylic diimide, 4,4 '-[(8,16-Dihydro-8,16-dioxodibenzo [a, j] perylene-2,10-diyl) di
- oxazine dye molecule examples include Cresyl violet, Oxazine 170, EVOblue 30, Nile Blue, and the like.
- carbopyronine dye molecules include CARBOPIRONIN 149.
- dipyrromethene (pyromesene) dye molecule examples include PYRROMETHENE650.
- Alexa Fluor dye molecules mainly having a rhodamine skeleton
- Alexa Fluor 555 Alexa Fluor 568, Alexa Fluor 594, Alexa Fluor 610, Alexa Fluor 633, Alexa Fluor 635, Alexa Fluor 635, Alexa Fluor 635 Alexa Fluor 680, Alexa Fluor 700, Alexa Fluor 750, etc. (manufactured by Invitrogen).
- BODIPY dye molecules mainly having a dipyrromethene skeleton
- BODIPY FL BODIPY TMR
- BODIPY TR BODIPY TR
- BODIPY 530/550 BODIPY 558/568
- BODIPY 564/570 BODIPY 589
- BODIPY 581/591 BODIPY 630/650
- BODIPY 650/665 manufactured by Invitrogen
- Cy-based dye molecule examples include Cy3.5, Cy5, Cy5.5 (manufactured by GE Healthcare).
- DY dye molecule (mainly having a rhodamine skeleton) include DY-590, DY-610, DY-615, DY-630, DY-631, DY-632, DY-633, DY-634 ( As mentioned above, manufactured by DYOMICS).
- HiLyte dye molecules include HiLyte 594, HiLyteFluor® TR (manufactured by Anaspec).
- DyLight-based dye molecule mainly having a rhodamine skeleton
- DyLight 594 mainly having a rhodamine skeleton
- DyLight 633 manufactured by Thermo Scientific
- ATTO dye molecules mainly having a rhodamine skeleton
- ATTO 590 ATTO 590
- ATTO 610 ATTO 610
- ATTO 620 ATTO 633
- ATTO 655 ATTO 655
- ATTO-TEC ATTO-TEC
- MFP dye molecules include MFP590 and MFP631 (manufactured by Mobitec).
- the dye examples include C-Phycocyanin, Phycocyanin, APC (Allophycocyanin), APC-XL, and NorthernLights 637 (manufactured by R & D Systems).
- the present invention among the above-mentioned known fluorescent dye molecules (compounds) or water-soluble processed products thereof, those satisfying the above-mentioned conditions (1) and (2), preferably the above-mentioned Stokes shift and / or Alternatively, it is possible to select and use one that satisfies the conditions regarding luminance. Such a selection can be made by a person skilled in the art without undue trial and error according to the condition determination method as described above.
- the desired emission wavelength of the fluorescent dye can be selected according to the application.
- the emission wavelength of the fluorescent dye is preferably from red to near infrared so as not to cover the emission wavelength of eosin that emits fluorescence.
- a fluorescent dye having an excitation maximum wavelength in the range of 555 to 620 nm and an emission maximum wavelength in the range of 580 to 770 nm is preferable.
- fluorescent dyes that can be used in the present invention are as follows, but the present invention is not limited to the embodiment using these fluorescent dyes.
- fluorescent dyes examples include 5-carboxy-X-rhodamine (5-ROX), Spectrum Red, Alexa Fluor 594, ATTO 590, DY-590, CAL Fluor Red 610. , DyLight 594.
- 5-carboxy-X-rhodamine 5-ROX
- Spectrum Red Alexa Fluor 594
- ATTO 590 ATTO 590
- DY-590 DY-590
- DyLight 594 examples include 5-carboxy-X-rhodamine (5-ROX), Spectrum Red, Alexa Fluor 594, ATTO 590, DY-590, CAL Fluor Red 610.
- fluorescent dyes examples include BODIPY 576/589, PYRROMETHENE 650, Cresyl violet, DY-610, and Texas Red.
- the resin constituting the fluorescent labeling resin particles of the present invention may be a thermosetting resin or a thermoplastic resin.
- a thermosetting resin such as a melamine resin that can fix the fluorescent dye inside a dense cross-linked structure is used.
- a resin containing (consisting of) is preferred.
- thermosetting resin is selected from the group consisting of melamine, urea, guanamines (including benzoguanamine, acetoguanamine, etc.), phenols (including phenol, cresol, xylenol, etc.), xylene, and derivatives thereof.
- the thing containing the structural unit formed from at least 1 type of monomer is mentioned. Any of these monomers may be used alone or in combination of two or more. If desired, a comonomer other than one or two or more of the above compounds may be used in combination.
- thermosetting resins include melamine / formaldehyde resins, urea / formaldehyde resins, benzoguanamine / formaldehyde resins, phenol / formaldehyde resins, and metaxylene / formaldehyde resins.
- thermosetting resins As a raw material for these thermosetting resins, not only the monomer itself as described above but also a prepolymer obtained by reacting a monomer with a compound such as formaldehyde or other crosslinking agent in advance may be used.
- a prepolymer obtained by reacting a monomer with a compound such as formaldehyde or other crosslinking agent in advance
- methylol melamine prepared by condensing melamine and formaldehyde under alkaline conditions is generally used as a prepolymer, and the compound is further alkyl etherified (in water).
- methylation for improving the stability of the resin butylated for improving the solubility in an organic solvent, etc.
- thermosetting resin may be one in which at least a part of hydrogen contained in the structural unit is replaced with a charged substituent or a substituent capable of forming a covalent bond.
- a thermosetting resin can be synthesized by using, as a raw material, a monomer in which at least one hydrogen is replaced by the above substituent (derivatized) by a known method.
- melamine resin, urea resin, benzoguanamine resin, etc. usually have cations generated from amino groups or sites derived therefrom, and phenol resins, xylene resins, etc. usually generate anions from hydroxyl groups or sites derived therefrom.
- thermosetting resin can be synthesized according to a known method.
- a melamine / formaldehyde resin can be synthesized by heating and polycondensing methylolmelamine prepared in advance as described above, with a reaction accelerator such as acid added as necessary.
- thermoplastic resin for example, at least one monofunctional monomer selected from the group consisting of styrene, (meth) acrylic acid and its alkyl ester, acrylonitrile, and derivatives thereof (involved in the polymerization reaction in one molecule).
- thermosetting resins include polystyrene, styrene resins composed of styrene and other monomers, polymethyl methacrylate, (meth) acrylic acid and alkyl esters thereof, and other acrylic resins composed of other monomers, poly Examples thereof include acrylonitrile, AS resin (acrylonitrile-styrene copolymer), ASA resin (acrylonitrile-styrene-methyl acrylate copolymer), acrylonitrile resin composed of acrylonitrile and other monomers.
- the thermoplastic resin is a structural unit formed from a polyfunctional monomer such as divinylbenzene (a group that participates in a polymerization reaction in one molecule, a monomer having two or more vinyl groups in the above example), that is, a crosslink.
- a site may be included.
- a cross-linked product of polymethyl methacrylate is exemplified.
- thermoplastic resin may be one in which at least a part of hydrogen contained in the structural unit is replaced with a substituent having a charge or a covalent bond.
- a thermoplastic resin can be synthesized by using, as a raw material, a monomer in which at least one hydrogen is replaced by the above substituent, such as 4-aminostyrene.
- the thermoplastic resin may contain a structural unit having a functional group for surface-modifying the obtained fluorescent labeling resin particles.
- a monomer such as glycidyl methacrylate having an epoxy group as a raw material
- fluorescent labeling resin particles having an epoxy group oriented on the surface can be prepared.
- This epoxy group can be converted to an amino group by reacting with excess ammonia water.
- Various amino molecules can be introduced into the amino group thus formed according to a known method (via a molecule that serves as a linker, if necessary).
- the fluorescent labeling resin particles of the present invention can be produced according to a known polymerization step (1) for various resins after using a fluorescent dye that satisfies specific conditions.
- the fluorescent labeling resin particles obtained by such a method may be further linked with a biologically relevant binding substance in the modification step (2).
- the encapsulated fluorescent labeling resin particles using a thermosetting resin can be basically produced according to an emulsion polymerization method, but are produced by the following polymerization step using a surfactant and a polymerization reaction accelerator. It is preferable.
- most of the fluorescent dye is desirably immobilized in a state where substantially all of the fluorescent dye is encapsulated in the resin particles. It is not excluded that the fluorescent dye is immobilized on the surface of the resin particle in a bound or attached state.
- the fluorescent dye is immobilized on the resin particles in the state where the fluorescent dye is encapsulated.
- a derivatization step for covalently bonding the resin raw material and the fluorescent dye in advance or for introducing a positively charged substituent into the resin raw material (that Even if such a step is not used, fluorescent labeling resin particles having excellent light emission intensity and light resistance can be obtained), but it is not excluded to use such a step in combination as desired.
- the polymerization step proceeds the polymerization reaction of the resin by heating a reaction mixture containing a fluorescent dye, a resin raw material (monomer or oligomer or prepolymer), preferably a surfactant and a polymerization reaction accelerator. This is a step of generating resin particles containing a fluorescent dye.
- the order of addition of each component contained in the reaction mixture is not particularly limited. Typically, an order is used in which a surfactant is added to an aqueous solution of a fluorescent dye, a resin raw material is added, and finally a polymerization reaction accelerator is added. Alternatively, the order may be such that the resin raw material is added to the surfactant aqueous solution, and then the polymerization reaction accelerator is added to advance the resin particle synthesis reaction while the fluorescent dye aqueous solution is added. It should be noted that the concentration of the aqueous solution of the specific fluorescent dye according to the present invention used in such a polymerization process can be adjusted within a relatively high range (for example, 250 to 450 ⁇ M) than the concentration of the aqueous solution of the conventional fluorescent dye. it can.
- Polymerization reaction conditions can be appropriately set in consideration of the type of resin and the composition of the raw material mixture.
- the reaction temperature is usually 70 to 200 ° C.
- the reaction time is usually 20 to 120 minutes.
- the reaction temperature is a temperature at which the performance of the fluorescent dye does not deteriorate (within a heat resistant temperature range).
- the heating may be performed in a plurality of stages. For example, the reaction may be performed at a relatively low temperature for a certain time, and then the temperature may be raised and the reaction may be performed at a relatively low temperature for a certain time.
- Surfactant A known emulsifier for emulsion polymerization can be used as the surfactant.
- Surfactants include anionic (anionic), nonionic (nonionic), and cationic (cationic) types. When synthesizing a (cationic) thermosetting resin having a positively charged substituent or moiety, it is preferable to use an anionic or nonionic surfactant. Conversely, when synthesizing a (anionic) thermosetting resin having a negatively charged substituent or moiety, it is preferable to use a cationic or nonionic surfactant.
- anionic surfactant examples include sodium dodecylbenzenesulfonate (product name “Neopelex” series, Kao Corporation).
- nonionic surfactants include polyoxyethylene alkyl ether-based (product name “Emulgen” series, Kao Corporation) compounds, polyvinylpyrrolidone (PVP), and polyvinyl alcohol (PVA).
- PVP polyvinylpyrrolidone
- PVA polyvinyl alcohol
- cationic surfactant examples include dodecyltrimethylammonium bromide.
- the particle diameter of the resin particles can be adjusted, and the coefficient of variation of the particle diameter is small, that is, it is possible to produce fluorescent labeling resin particles having a uniform particle size. it can.
- the amount of the surfactant added is, for example, 10 to 60% by weight based on the resin raw material, or 0.1 to 3.0% by weight based on the entire raw material mixture.
- the polymerization reaction accelerator accelerates the polycondensation reaction of a thermosetting resin such as a melamine resin, and charges the functional group such as an amino group contained in the resin or fluorescent dye by adding a proton (H + ). , Has a function of facilitating electrostatic interaction.
- a polymerization reaction accelerator include acids such as formic acid, acetic acid, sulfuric acid, paratoluenesulfonic acid, and dodecylbenzenesulfonic acid.
- the fluorescent dye is a compound having a carboxyl group or a sulfo group, the fluorescent dye can also donate a proton in the same manner as the above acid.
- the modification step performed as necessary is a step for connecting a biologically relevant binding substance or the like to the surface of the fluorescent labeling resin particle according to the use of the fluorescent labeling resin particle.
- a fluorescent label one reactive functional group present on the surface
- a biologically relevant binding substance the other reactive functional group present in the molecule
- bonded directly it can also couple
- Such a reaction can be performed by adding necessary reagents and allowing a predetermined time to elapse.
- an amino group is introduced by reacting a fluorescent labeling resin particle having a hydroxyl group on the surface with a silane coupling agent (for example, aminopropyltrimethoxysilane), while a thiol group introducing reagent (for example, N is added to streptavidin).
- a silane coupling agent for example, aminopropyltrimethoxysilane
- a thiol group introducing reagent for example, N is added to streptavidin.
- -Succimidyl S acetylthioacetic acid is reacted to introduce a thiol group
- a PEG (polyethylene glycol) based linker molecule having a maleimide group that is reactive with both an amino group and a thiol group at both ends is reacted.
- a method of linking fluorescent labeling resin particles and streptavidin is reacted.
- an epoxy group derived from the monomer appears on the surface of the resin particle for fluorescent labeling.
- the epoxy group can be converted into an amino group, and a desired biologically relevant binding substance or the like can be further linked to the amino group.
- thermosetting resin particles containing no fluorescent dye are prepared in the same manner as in the polymerization step (1) of the encapsulated fluorescent labeling resin particles described above, except that the fluorescent dye is not used as a raw material. Subsequently, the obtained resin particles (its dispersion liquid) and a fluorescent dye (its aqueous solution) are mixed, and the fluorescent dye is adsorbed on the surface of the resin particles.
- the thermosetting resin and the fluorescent dye of the resin particles preferably have substituents or sites having opposite charges and are adsorbed by electrostatic interaction.
- thermoplastic resin inclusion type
- the thermoplastic resin can be synthesized according to a known method such as radical polymerization or ionic polymerization (anionic polymerization, etc.).
- Encapsulated fluorescent labeling resin particles using a thermoplastic resin can also be produced according to these methods, but it is preferred to produce them by, for example, a polymerization step according to a soap-free emulsion polymerization method.
- the polymerization step (1) typically involves heating a reaction mixture containing a fluorescent dye, a resin raw material, and a polymerization initiator (benzoyl peroxide, azobisisobutyronitrile, etc.) to form a resin.
- a polymerization initiator benzoyl peroxide, azobisisobutyronitrile, etc.
- the polymerization initiator and polymerization reaction conditions can be appropriately set in consideration of the type of resin.
- the reaction temperature is usually 20 to 150 ° C.
- the reaction time is usually 10 to 240 minutes.
- thermoplastic resin particles not containing a fluorescent dye are prepared in the same manner as in the polymerization step (1) of the encapsulated fluorescent labeling resin particles described above, except that the fluorescent dye is not used as a raw material. Subsequently, the obtained resin particles (its dispersion liquid) and a fluorescent dye (its aqueous solution) are mixed, and the fluorescent dye is adsorbed on the surface of the resin particles.
- the thermoplastic resin and the fluorescent dye of the resin particle preferably have substituents or sites having opposite charges and are adsorbed by electrostatic interaction.
- ⁇ Use of resin particles for fluorescent labeling (Fluorescent labeling agent)
- the use of the resin particle for fluorescent labeling of the present invention is not particularly limited, but typically, the detection target substance contained in a sample (tissue section) is labeled so that fluorescence observation can be performed in immunostaining.
- the substance to be detected is not particularly limited, but in pathological diagnosis, an antigen is generally selected according to the purpose.
- an antigen is generally selected according to the purpose.
- HER2 can be used as a detection target substance in pathological diagnosis related to breast cancer.
- an antibody that specifically binds to the detection target substance
- the complex composed of the resin particles for fluorescent labeling and the primary antibody can be directly bound to the detection target substance and fluorescently labeled (primary antibody method).
- an antibody that binds to an antibody (primary antibody) that specifically binds to the substance to be detected
- the primary antibody is an antibody (IgG) produced from a rabbit
- the secondary antibody is an anti-rabbit IgG antibody.
- the detection target substance can be indirectly fluorescently labeled by binding a complex composed of the fluorescent dye-immobilized resin particles and the secondary antibody to the primary antibody bound to the detection target substance (secondary antibody). Law).
- the secondary antibody is complexed with a substance that can bind to the substance complexed with the fluorescent labeling resin particles.
- a complex of resin particles for fluorescent labeling and avidin or streptavidin is used, a complex of a secondary antibody and biotin is used in combination.
- a secondary antibody and biotin complex is bound to the primary antibody bound to the detection target substance, and a complex composed of a fluorescent dye-immobilized resin particle and avidin or streptavidin is further bound to the complex.
- the substance to be detected can be indirectly fluorescently labeled (biotin-avidin method or sandwich method).
- a complex of fluorescent labeling resin particles and biotin can be used in combination with a complex of a secondary antibody and avidin or streptavidin.
- the primary antibody may be selected according to the selected substance to be detected, which specifically binds to it.
- an anti-HER2 monoclonal antibody can be used as the primary antibody.
- Such a primary antibody can be produced by a general technique using mice, rabbits, cows, goats, sheep, dogs, chickens and the like as immunized animals.
- the secondary antibody may be selected in accordance with the selected primary antibody.
- the primary antibody is a rabbit anti-HER2 monoclonal antibody
- an anti-rabbit IgG antibody can be used as the secondary antibody.
- Such secondary antibodies can also be produced by general techniques.
- nucleic acid molecule having a base sequence complementary to the nucleic acid molecule as a biologically relevant binding substance corresponding to the detection target substance as a nucleic acid molecule.
- the complex in which the biologically relevant binding substance is linked to the fluorescent labeling resin particles may be prepared by any known technique. For example, amidation by reaction of amine and carboxylic acid, sulfidation by reaction of maleimide and thiol, imination by reaction of aldehyde and amine, amination by reaction of epoxy and amine can be used.
- the functional group involved in such a reaction may be one existing in advance on the surface of the resin particle (functional group derived from the raw material monomer of the resin), or a known functional group existing on the surface of the resin particle. It may be a functional group converted according to a technique or a functional group introduced by surface modification or the like. An appropriate linker molecule may be used as necessary.
- a tissue immunostaining kit using the fluorescent labeling resin particles of the present invention includes at least the fluorescent labeling resin particle of the present invention, a complex in which a biorelevant binding substance is linked to the fluorescent labeling resin particle, or the fluorescent labeling resin particle for preparing the complex, the biorelevant binding Includes chemical substances and reagents.
- the kit further includes a primary antibody, a secondary antibody, another biologically relevant binding substance (for example, biotin) used in combination with the biologically relevant binding substance (for example, streptavidin), and a desired complex as necessary. Reagents for forming and other reagents used for immunohistochemical staining may be included.
- the fluorescent labeling agent using the fluorescent labeling resin particles of the present invention is, for example, a biological substance detection method using immunostaining and morphological observation staining in combination in order to enhance information, and more specifically, (1) a fluorescent label A step of immunostaining a tissue section using (2) a step of observing and staining a tissue section using a staining agent for morphological observation, and (3) a step of fluorescently observing the tissue section after staining. It can be used in the method for detecting a biological material containing. Note that either the immunostaining step (1) or the morphological observation staining step (2) may be performed first.
- the tissue section is subjected to deparaffinization and antigen activation according to conventional methods.
- the detection target substance can be appropriately labeled according to the form of the fluorescent labeling agent (complex of fluorescent labeling resin particles and biologically relevant binding substance) as described above. What is necessary is just to add a required substance to a tissue slice sequentially, and to make it react.
- the fluorescent labeling agent complex of fluorescent labeling resin particles and biologically relevant binding substance
- the morphological observation staining step (2) can be performed according to a conventional method.
- staining using eosin in which cytoplasm, stroma, various fibers, erythrocytes, and keratinocytes are stained from red to dark red
- eosin in which cytoplasm, stroma, various fibers, erythrocytes, and keratinocytes are stained from red to dark red
- hematoxylin in which cell nuclei, lime, cartilage tissue, bacteria, and mucus are stained blue-blue to light blue
- the method of performing these two stainings simultaneously is hematoxylin, Known as eosin staining (HE staining)).
- a dehydration treatment immersed in ethanol a penetration treatment immersed in an organic solvent such as xylene, and an encapsulation treatment using an encapsulant are performed.
- the tissue section that has been subjected to immunostaining and morphological observation staining by the above-described step is irradiated with excitation light having an appropriate wavelength according to the fluorescent dye used, whereby the fluorescent labeling is performed. Observe the fluorescence emitted by the body.
- a predetermined biomolecule such as an antigen present in the tissue section can be detected, and a molecular target drug (for example, an antibody drug “Herceptin” (trademark) which is a humanized anti-HER2 monoclonal antibody) It can be used as information for determining the suitability of application.
- a molecular target drug for example, an antibody drug “Herceptin” (trademark) which is a humanized anti-HER2 monoclonal antibody
- irradiation of excitation light it is only necessary to use the same irradiation means as in general fluorescence observation, for example, using a filter that selectively transmits a predetermined wavelength as necessary from a laser light source provided in a fluorescence microscope, What is necessary is just to irradiate the dye
- the fluorescence observation may be performed from a lens barrel of a fluorescence microscope, or may be performed by separately displaying an image taken by a camera installed on the fluorescence microscope on a display means (a monitor or the like).
- both the immunostaining and the morphological observation staining are applied to the same tissue section, but when observing an image by the morphological observation staining, the fluorescent dye for immunostaining is excited. It is not necessary to irradiate the excitation light, and the observation may be performed under the same observation conditions as the optical microscope.
- Fluoramine-encapsulated melamine resin Particulate surfactant was added to 22 mL of particle water so that it might become 0.5 vol%.
- An untreated product or a water-solubilized product of a predetermined fluorescent dye was added to the solution so as to have a predetermined concentration as a charged amount, and the mixture was heated to 70 ° C. while stirring on a hot stirrer.
- a predetermined amount of a predetermined melamine resin raw material (such as MX-035) was added, and a predetermined amount of a predetermined polymerization reaction accelerator having a concentration of 10 vol% was further added.
- a predetermined amount of a predetermined melamine resin raw material such as MX-035
- a predetermined amount of a predetermined polymerization reaction accelerator having a concentration of 10 vol% was further added.
- the temperature was raised to 90 ° C. and the reaction was performed by heating and stirring for 20 minutes, whereby fluorescent dye-encapsulated melamine resin particles were generated.
- the dispersion was centrifuged (20000 G for 15 minutes) to collect particles, washed with ultrapure water and purified.
- Fluorescent dye-encapsulated urea resin particles A predetermined surfactant was added to 22 mL of water in an amount of 0.5 vol%. An untreated product or a water-solubilized product of a predetermined fluorescent dye was added to the solution so as to have a predetermined concentration as a charged amount, and the mixture was heated to 70 ° C. while stirring on a hot stirrer. Subsequently, a predetermined amount of urea was added, and further a predetermined amount of formalin having a concentration of 10 vol% was added. After heating and stirring at 70 ° C.
- the temperature was raised to 90 ° C., and the reaction was performed by heating and stirring for 20 minutes, whereby fluorescent dye-encapsulated urea resin particles were generated.
- the dispersion was centrifuged (20000 G for 15 minutes) to collect particles, washed with ultrapure water and purified.
- an aqueous solution of 0.1 nM concentration of fluorescent labeling resin particles was prepared, and its emission intensity was measured with a fluorescence spectrophotometer F-7000 manufactured by Hitachi (measurement conditions: Photovoltaic voltage 300 V, excitation light slit 20 nm, emission slit 5 nm). ).
- this aqueous solution was diluted to 0.01 nM, and 5 ⁇ L was spread on APS coated glass.
- xanthane encapsulant Enteran New manufactured by Merck & Co., Ltd. was added dropwise and covered with a cover glass.
- the APS-coated glass was placed on the stage of a fluorescence microscope and excited at the maximum excitation wavelength of ⁇ 5 nm of the fluorescent dye, and a fluorescence image was acquired (initial emission intensity). Thereafter, after continuing to irradiate the excitation light for 10 minutes, a fluorescence image after 10 minutes was obtained. The relative value of the light emission intensity after 10 minutes to the initial light emission intensity was calculated.
Abstract
Description
[項1]
下記(1)および(2)の両方の条件を満たす蛍光色素が樹脂粒子に固定化されていることを特徴とする、蛍光標識用樹脂粒子。
(1)水溶液中の蛍光色素単独の濃度に依存する発光強度のピークが、30~80μM(M:モル濃度 モル/リットル)の範囲内にあること。
(2)濃度100μMにおける発光強度が前記ピークの発光強度の80%以上であること。
[項2]
前記蛍光色素の水溶液中でのストークスシフトが25nm以上である、項1に記載の蛍光標識用樹脂粒子。
[項3]
前記蛍光色素が水溶化処理されたものである、項1または2に記載の蛍光標識用樹脂粒子。
[項4]
前記水溶化処理が酸処理である、項3に記載の蛍光標識用樹脂粒子。
[項5]
前記蛍光色素の水溶液中でのピーク発光強度が、下記式で表される輝度として100,000以上である、項1~4のいずれか一項に記載の蛍光標識用樹脂粒子。
輝度=モル吸光係数×量子収率×1/1000
[項6]
前記蛍光色素が熱硬化性樹脂を含有する樹脂粒子に内包されている、項1~5のいずれか一項に記載の蛍光標識用樹脂粒子。
[項7]
粒子表面に生体関連結合性物質が連結されている、項1~6のいずれか一項に記載の蛍光標識用樹脂粒子。
蛍光標識用樹脂粒子は、複数の蛍光色素分子が化学的または物理的な作用により樹脂粒子に固定化された構造を有する物質を総称する用語であり、その形態は特に限定されるものではない。
本発明における蛍光色素は、(1)水溶液中の蛍光色素単独の濃度に依存する発光強度のピークが、30~80μMの範囲内にあること、および(2)濃度100μMにおける発光強度が前記ピークの発光強度の80%以上であること、の両方の条件を満たすものであれば、特に限定されるものではない。
本発明で用いる蛍光色素の上記式で表れる輝度は、用途に応じた適切な蛍光強度を有する蛍光標識用樹脂粒子を作製できるものであればよく、特に限定されるものではない。たとえば、前述したような発光強度のピークにおける輝度が100,000以上の蛍光色素は、そのような蛍光標識用樹脂粒子を作製しやすいため好ましい。
本発明の蛍光標識用樹脂粒子を構成する樹脂は、熱硬化性樹脂であっても、熱可塑性樹脂であってもよい。たとえば、キシレンのような有機溶媒を用いる透徹工程において蛍光色素が溶出しにくいという観点からは、緻密な架橋構造の内部に蛍光色素を固定化することができる、メラミン樹脂等の熱硬化性樹脂を含有する(のみからなる)樹脂が好ましい。
本発明の蛍光標識用樹脂粒子は、蛍光色素として特定の条件を満たすものを用いた上で、各種の樹脂について公知の重合工程(1)に準じて製造することができる。また、そのような方法により得られた蛍光標識用樹脂粒子は、さらに修飾工程(2)により、生体関連結合性物質を連結させてもよい。
熱硬化性樹脂を用いた内包型の蛍光標識用樹脂粒子は、基本的に乳化重合法に従って製造することができるが、界面活性剤および重合反応促進剤を用いる次のような重合工程により製造することが好ましい。なお、このような製造方法により得られる内包型の蛍光標識用樹脂粒子において、蛍光色素の大部分が、望ましくは実質的に全てが樹脂粒子に内包された状態で固定化されるが、一部の蛍光色素が樹脂粒子の表面に結合ないし付着した状態で固定化されることが排除されるものではない。また、蛍光色素が内包された状態において、どのような化学的または物理的な作用で蛍光色素が樹脂粒子に固定化されているかは限定されるものではない。本発明では、重合工程に先立って、樹脂原料と蛍光色素とをあらかじめ共有結合させたり、樹脂原料に積極的に荷電した置換基を導入したりするための誘導体化工程を設ける必要はない(そのような工程を用いなくても、発光強度や耐光性に優れた蛍光標識用樹脂粒子が得られる)が、所望によりそのような工程を併用することも排除されるものではない。
重合工程は、蛍光色素、樹脂原料(モノマーまたはオリゴマーないしプレポリマー)、好ましくはさらに界面活性剤および重合反応促進剤を含有する反応混合物を加熱して樹脂の重合反応を進行させ、蛍光色素を内包する樹脂粒子を生成させる工程である。
界面活性剤としては、公知の乳化重合用乳化剤を用いることができる。界面活性剤には、アニオン系(陰イオン系)、ノニオン系(非イオン系)、カチオン系(陽イオン系)のものがある。正に荷電した置換基ないし部位を有する(カチオン系の)熱硬化性樹脂を合成する場合は、アニオン系またはノニオン系の界面活性剤を用いることが好ましい。逆に負に荷電した置換基ないし部位を有する(アニオン系の)熱硬化性樹脂を合成する場合は、カチオン系またはノニオン系の界面活性剤を用いることが好ましい。
重合反応促進剤は、メラミン樹脂等の熱硬化性樹脂の重縮合反応を促進するとともに、当該樹脂または蛍光色素に含まれるアミノ基のような官能基にプロトン(H+)を付与して荷電させ、静電的相互作用を起こしやすくする機能を有する。熱硬化性樹脂の反応は加温のみでも進行するが、重合反応促進剤を加えるとより低温で進行するので、反応や性能を制御できる範囲で添加することができる。このような重合反応促進剤としては、たとえば、ギ酸、酢酸、硫酸、パラトルエンスルホン酸、ドデシルベンゼンスルホン酸などの酸が挙げられる。なお、蛍光色素がカルボキシル基やスルホ基を有する化合物である場合、当該蛍光色素も上記の酸と同様にプロトンを供与することもできる。
必要に応じて行われる修飾工程は、蛍光標識用樹脂粒子の用途に応じて、蛍光標識用樹脂粒子の表面に生体関連結合性物質などを連結するための工程である。
熱硬化性樹脂を用いた吸着型の蛍光標識用樹脂粒子の製造方法は次のようなものとなる。まず、蛍光色素を原料として配合しないこと以外は上述した内包型の蛍光標識用樹脂粒子の重合工程(1)と同様にして、蛍光色素を含有しない熱硬化性樹脂粒子を調製する。続いて、得られた樹脂粒子(その分散液)と蛍光色素(その水溶液)とを混合し、当該樹脂粒子の表面に蛍光色素を吸着させる。この場合、樹脂粒子の熱硬化性樹脂および蛍光色素は、互いに反対の電荷を有する置換基ないし部位を有しており、静電的相互作用により吸着するものであることが好適である。
熱可塑性樹脂は、ラジカル重合、イオン重合(アニオン重合、他)など、公知の手法に従って合成することができる。熱可塑性樹脂を用いた内包型の蛍光標識用樹脂粒子もそれらの手法に準じて製造することができるが、たとえば、ソープフリー乳化重合法に従った重合工程により製造することが好ましい。
熱可塑性樹脂を用いた吸着型の蛍光標識用樹脂粒子の製造方法は次のようなものとなる。まず、蛍光色素を原料として配合しないこと以外は上述した内包型の蛍光標識用樹脂粒子の重合工程(1)と同様にして、蛍光色素を含有しない熱可塑性樹脂粒子を調製する。続いて、得られた樹脂粒子(その分散液)と蛍光色素(その水溶液)とを混合し、当該樹脂粒子の表面に蛍光色素を吸着させる。この場合、樹脂粒子の熱可塑性樹脂および蛍光色素は、互いに反対の電荷を有する置換基ないし部位を有しており、静電的相互作用により吸着するものであることが好適である。
(蛍光標識剤)
本発明の蛍光標識用樹脂粒子の用途は特に限定されるものではないが、典型的には、試料(組織切片)に含まれる検出対象物質を標識し、免疫染色において蛍光観察できるようにするための、蛍光標識剤としての用途が挙げられる。すなわち、上述したような本発明の蛍光標識用樹脂粒子は、免疫染色の実施形態に応じた生体関連結合性物質を連結させて、複合体(コンジュゲート)として使用することが好適である。
本発明の蛍光標識用樹脂粒子を用いた蛍光標識剤は、たとえば、情報性を高めるために免疫染色と形態観察染色を併用する生体物質検出方法、より具体的には、(1)蛍光標識体を用いて組織切片を免疫染色する工程と、(2)形態観察用の染色剤を用いて組織切片を形態観察染色する工程と、(3)染色後の組織切片を蛍光観察する工程、とを含む生体物質検出方法において使用することができる。なお、(1)の免疫染色工程および(2)の形態観察染色工程はどちらを先におこなってもよい。
・エポスター(登録商標)(株式会社日本触媒)
MX030w:ポリメタクリル酸メチル系架橋物(エマルション濃度7.5~10%、平均粒径0.03~0.05μm)
・ニカラック(登録商標)(株式会社三和ケミカル)
MX-035:メチル化メラミン樹脂(重量平均重合度1.5)
MS-11:メチル化メラミン樹脂(重量平均重合度1.8)
MX-410:メチル/nーブチル化メラミン樹脂(重量平均重合度1.8)
MW-022:メチル化メラミン樹脂(重量平均重合度1.8)
MX-730:メチル化メラミン樹脂(重量平均重合度2.4)
・エマルゲン(登録商標)(花王株式会社)
エマルゲン 150:ポリオキシエチレンラウリルエーテル、有効成分量:100%
エマルゲン 430:ポリオキシエチレンオレイルエーテル、有効成分量:100%
・ネオペレックス(登録商標)(花王株式会社)
ネオペレックス G-15:ドデシルベンゼンスルホン酸ナトリウム、有効成分量:16%
・その他
PVP30000:ポリビニルピロリドン(重合度約30000)
PVA2000:ポリビニルアルコール(重合度約2000)
PVA500:ポリビニルアルコール(重合度約500)
[蛍光色素の水溶化処理]
蛍光色素を100mg程度用意し、濃硫酸、濃塩酸、酢酸、ギ酸、ホルムアルデヒドまたはアセトアルデヒドを100mL添加して、水溶化処理を行った。その後、飽和NaCl溶液を添加し、水溶化処理された蛍光色素を塩析させ、0.1μmの濾紙を用いた濾過により回収した。
下記表に示す蛍光色素(水溶化処理物および無処理物を含む)について、それぞれの分子量から算出した必要量が溶解した400μMの水溶液(ミリQ希釈)を調製し、さらにこの水溶液を希釈して200,100,90,85,50,75,50,65,60,55,50,45,40,30,20μMの水溶液を調製した。各濃度の蛍光色素水溶液の発光強度を、Hitachi社製蛍光光度計F-7000で測定した(測定条件:ホトマル電圧300V、励起光スリット20nm、発光スリット5nm)。代表的な5種類の蛍光色素の無処理物および水溶化処理物についての結果を図1に示す。この測定結果より、(1)発光強度がピークとなるときの濃度、および(2)そのピーク発光強度に対する濃度100μM時の発光強度の比率(発光強度比)を求めた。これらの結果を下記表に示す。本発明で規定する(1)の条件(発光強度のピークが30~80μMの範囲内にある)および(2)の条件(濃度100μMにおける発光強度が前記ピークの発光強度の80%以上である)の両方を満たす蛍光色素を実施例、そうでない蛍光色素を比較例として取り扱うことにした。
各実施例および比較例の蛍光標識用樹脂粒子を、下記表に示す所定の条件に従って作製した。具体的には次の通りである。
水22mLに所定の蛍光色素の無処理物または水溶化処理物を、仕込み量として所定の濃度になるように添加し、ホットスターラー上で撹拌しながら50℃に加熱した。続いて、所定の架橋PMMA原料(MX030w)を所定量添加し、さらに10vol%の濃度の所定の重合反応促進剤等を所定量添加した。50℃で20分間加熱撹拌して反応させることで、蛍光色素内包架橋PMMA粒子が生成した。この分散液を遠心分離にかけて(20000Gで15分間)粒子を回収し、超純水で洗浄して精製した。
水22mLに所定の界面活性剤が0.5vol%となるように添加した。その溶液に所定の蛍光色素の無処理物または水溶化処理物を、仕込み量として所定の濃度になるように添加し、ホットスターラー上で撹拌しながら70℃に加熱した。続いて、所定のメラミン樹脂原料(MX-035等)を所定量添加し、さらに10vol%の濃度の所定の重合反応促進剤を所定量添加した。70℃で50分間加熱撹拌した後、90℃に昇温して20分間加熱撹拌して反応させることで、蛍光色素内包メラミン樹脂粒子が生成した。この分散液を遠心分離にかけて(20000Gで15分間)粒子を回収し、超純水で洗浄して精製した。
水22mLに所定の界面活性剤が0.5vol%となるように添加した。その溶液に所定の蛍光色素の無処理物または水溶化処理物を、仕込み量として所定の濃度になるように添加し、ホットスターラー上で撹拌しながら70℃に加熱した。続いて、尿素を所定量添加し、さらに10vol%の濃度のホルマリンを所定量添加した。70℃で50分間加熱撹拌した後、90℃に昇温して20分間加熱撹拌して反応させることで、蛍光色素内包尿素樹脂粒子が生成した。この分散液を遠心分離にかけて(20000Gで15分間)粒子を回収し、超純水で洗浄して精製した。
実施例および比較例で作製した蛍光標識用樹脂粒子について、カールツァイス社製蛍光顕微鏡Axio Imager Z2の観察条件下(励起波長:最大励起波長-5nm、40倍、10分)における耐光性を評価した。
Claims (7)
- 下記(1)および(2)の両方の条件を満たす蛍光色素が樹脂粒子に固定化されていることを特徴とする、蛍光標識用樹脂粒子。
(1)水溶液中の蛍光色素単独の濃度に依存する発光強度のピークが、30~80μMの範囲内にあること。
(2)濃度100μMにおける発光強度が前記ピークの発光強度の80%以上であること。 - 前記蛍光色素の水溶液中でのストークスシフトが25nm以上である、請求項1に記載の蛍光標識用樹脂粒子。
- 前記蛍光色素が水溶化処理されたものである、請求項1または2に記載の蛍光標識用樹脂粒子。
- 前記水溶化処理が酸処理である、請求項3に記載の蛍光標識用樹脂粒子。
- 前記蛍光色素の水溶液中でのピーク発光強度が、下記式で表される輝度として100,000以上である、請求項1~4のいずれか一項に記載の蛍光標識用樹脂粒子。
輝度=モル吸光係数×量子収率×1/1000 - 前記蛍光色素が熱硬化性樹脂を含有する樹脂粒子に内包された状態で固定化されている、請求項1~5のいずれか一項に記載の蛍光標識用樹脂粒子。
- 粒子表面に生体関連結合性物質が連結されている、請求項1~6のいずれか一項に記載の蛍光標識用樹脂粒子。
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WO2016203952A1 (ja) * | 2015-06-16 | 2016-12-22 | コニカミノルタ株式会社 | 病理標本、病理標本の作製方法、および蛍光画像の取得方法 |
WO2017109828A1 (ja) * | 2015-12-21 | 2017-06-29 | コニカミノルタ株式会社 | 色素含有熱硬化性樹脂粒子の製造方法 |
WO2018155694A1 (ja) * | 2017-02-27 | 2018-08-30 | 富士フイルム株式会社 | 病理診断用染色剤、細胞核染色方法、病理標本の製造方法、及び色素 |
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JP6881564B2 (ja) * | 2017-02-27 | 2021-06-02 | コニカミノルタ株式会社 | 粒子表面状態の評価方法および評価システム |
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Also Published As
Publication number | Publication date |
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US20160011179A1 (en) | 2016-01-14 |
JP6296050B2 (ja) | 2018-03-20 |
JP2018119971A (ja) | 2018-08-02 |
JP6536706B2 (ja) | 2019-07-03 |
EP2966435A1 (en) | 2016-01-13 |
JPWO2014136776A1 (ja) | 2017-02-16 |
EP2966435A4 (en) | 2016-11-09 |
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