WO2014017804A1

WO2014017804A1 - Composition comprising natural extracts as active ingredients for improving condition of hair and scalp, and method for preparing same

Info

Publication number: WO2014017804A1
Application number: PCT/KR2013/006585
Authority: WO
Inventors: 방준석; 김두영; 고찬영; 이재걸
Original assignee: 현대약품 주식회사
Priority date: 2012-07-24
Filing date: 2013-07-23
Publication date: 2014-01-30

Abstract

The present invention relates to a composition comprising natural extracts as active ingredients for improving the condition of the hair and scalp, to a method for preparing same, and to a method for improving the condition of the hair and scalp by applying natural extracts thereto. The composition for improving the condition of the hair and scalp according to the present invention comprises natural extracts as active ingredients, and therefore the composition of the present invention is free of side effects or toxicity, and can be applied in an economically advantageous and easy manner as the composition does not require a surgical procedure.

Description

[Specification】

[Name of invention]

Composition for improving hair and scalp condition comprising natural extract as an active ingredient and method for producing same

Technical Field

The present invention relates to a composition for improving the condition of hair and scalp comprising a natural extract as an active ingredient and a method for producing the same, and also to a method for improving the condition of hair and scalp by administering the natural extract of the present invention.

Background Art

The most common type of alopecia, male alopecia, is testosterone, one of the male hormones.

5 α-reductase) is converted to dihydrotestosterone (DHT), an active male hormone. The activated dehydrotestosterone binds to androgen receptors to delay the hair follicle cell protein synthesis, shortening the hair follicle growth period and contracting the hair follicles, causing hair loss. In addition, sebum is produced excessively during androgenetic alopecia, and as a result, scalp may be accompanied by inflammatory hair loss [Dennis A. Holt. et. al. J Med. Chem. , 33, 943. (1990).

A common treatment for hair loss is hair transplantation. In other words. It is a method of transplanting a plug of hairy skin from a scalp where hair grows to a bald area. This method is time consuming, relatively painless, and expensive. Other non-drug therapies include ultraviolet radiation and exercise therapy.

Another treatment for hair loss is drug therapy. Merck of the United States

5—A drug that inhibits the action of alpha-reductase to inhibit the production of dihydrotestosterone (DHT). Hair restorers prepared for oral administration of finasteride are commercially available. However, there is a problem that side effects occur due to steroidal drugs.

Dyezein is a type of phytoestrogen. Equol, the major metabolite of (daidzein), blocks the pathway of the male hormone dihydrotertosterone (DHT) by binding directly to dihydrotertosterone (DHT) without affecting testosterone. This has been reported. Therefore, there is a need to develop a therapeutic agent derived from natural products that is economical and does not have various side effects or toxicity concerns.

[Detailed Description of the Invention]

[Technical problem]

In order to solve the above problems, the present inventors have experimented with Root, Peony, Fuxin, Stone, Jenny, Senna Leaf, Rosewood, Excess, Taxa, Spectator, Flax Seed, Gallium, Turmeric, Gluluin, Mine, and Joiner extract. It was confirmed that promoting the growth of hair by inhibiting the efficacy of the 5-alpha-reductase enzyme. From the extract, it is interesting to find a composition for improving the condition of hair and scalp and a method for improving the condition of hair and scalp.

Technical Solution

It is an object of the present invention to provide a composition for improving hair and scalp conditions and a method for producing the same, including a natural extract as an active ingredient.

In addition, the present invention hair loss at least one extract selected from the group consisting of Root, Peony, Fuxin, Stone Jenny, Senna Leaf, Rosewood, Excess, Taxa, Spectator, Flax Seed, Brown, Turmeric, Gluluin, Mine and Joiner It is an object of the present invention to provide a method for improving hair and scalp conditions, comprising administering to a subject having a subject. [Favorable effect]

According to the present invention, a composition for improving hair and scalp condition comprising a natural extract having an excellent inhibitory effect on 5-alpha-reductase (testosterone 5 a -reductase) as an active ingredient, a method for preparing the same, and a natural extract for hair and scalp conditions A method for improving the is provided. The hair and scalp condition improvement composition contains a natural extract as an active ingredient, there is no fear of side effects or toxicities do not require the procedure can be economically and easily applied. [Brief Description of Drawings]

1 is ^. Experimental Examples of the Invention; The extracted ion chromatograms of i¾ testosterone obtained according to Example 1 ((a): Reference standard, (b): Microsomal fract ion).

ᅳ Figure 2 is the Extracted one ion of testosterone obtained according to Experimental Example 1 of the present invention. chromatograms ((a) :. Reference standard, ^' _. (b): Microsomal fract ion).

3 is' Extracted—ion chromatograms of i / _3- (lihydrotestosterone (<1 DHT) obtained according to Experimental Example 1 of the present invention ((a): Reference standard, (b): Microsomal fraction).

4 is Extracted ion chromatograms of dihydrotestosterone (DHT) obtained according to Experiment 1 of the present invention ((a): Reference standard, (, b) ^: Microsomal fract ion).

Figure 5 shows the growth of hair over time obtained according to Experimental Example 4 of the present invention.

[Best form for implementation of the invention]

In order to achieve the above object, a preferred embodiment of the present invention, Root, Peony, Bokshin, Seok, Jenny, Senna Leaf, Rosewood, Excess, Taxa, Vasculature, Flax Seed, Brownish, Turmeric, Gluluin, Mine and It provides a composition for improving hair and scalp conditions comprising at least one extract selected from the group consisting of joiner as an active ingredient.

In addition, another preferred embodiment of the present invention provides a composition for hair, comprising the composition.

In addition, another preferred embodiment of the present invention, Root, Peony, Fuxin, Stone, Jenny. Hair and scalp conditions comprising administering to the subject with hair loss at least one extract selected from the group consisting of senna leaf, rosewood, excess, taxa, spectators, flaxseed, brownish, turmeric, palluene, mein, and joiner Provide ways to improve. _. In addition, another preferred embodiment of the present invention, in the group consisting of Root, Peony, Fuxin, Stone, Jenny, Senna Leaf, Rosewood, Excess, Taxa, Spectator, Flax Seed, Gallium, Turmeric, Jagruin, Mine and Joiner Extracting at least one selected from water, ethane, or a mixed solvent thereof;

Filtering the extract obtained through the extraction step under reduced pressure; And

. It provides a method for producing a composition for improving the hair and scalp conditions comprising the step of concentrating the filtrate obtained through the filtration step under reduced pressure.

Hereinafter, the present invention will be described in more detail.

_ 5-alpha reductase by a switch (testosterone 5 a-reductase) is a namseongho ^ Te ^ monohydrate testosterone (testosterone) activity of dihydro testosterone (dihydrotestosterone, DHT) ^'type of androgen-driven and, thus de-activated Hydrotestosterone binds to androgen receptors, delays the synthesis of protein in the hair follicle cells, shortens the hair follicle's growth period and atrophies the hair follicles, causing hair loss. Accompanying hair loss may also appear [Dennis A. Holt, et. al, J Med. Chem. 33, 943, (1990)].

Therefore, by inhibiting the 5-alpha-reductase, hair loss can be prevented and hair growth can be promoted, and sebum secretion and dandruff can be prevented or reduced, and scalp inflammation can also be prevented or improved (Olsen EA, Hordinsky M). , Whiting D, et al "The importance of dual ^' 5a Ipha 一 reductase inhibition in the treatment of MPB: results of a randomized lacebo-control led study of dutaster icle versus finasteride"-/ Am Acad Derma tol. 55 (6), 2006, Leyden, James; Dun lap, Frank; Mi Her, Bruce; Winters, Peter; Lebwohl, Mark; Hecker, David; Kraus, Stephen; Baldwin, Hi l.ary et al. "Finasteride in the treatment of men with frontal male pattern hair loss ".Journal of the American Academy of Dermatology 40 (6 Pt 1): 930-7., 1999). ^'

The present inventors have hair, and the new natural product derived from preparation of the form no scalp improvement with regard to the unwanted side effects can be substituted for conventional medicaments frequent, side effect to toxic ⁱ with a superior effect to the hair and scalp improves As a result of research for the development, extracts of certain herbs and It confirmed its decomposition hwakmul the 5-alpha reductase (testosterone 5a-reductase> inhibitory activity is significantly excellent to thereby completing the present invention. ^"

As used herein, the term 'extract' refers to an extract obtained from various organs of each medicine, such as leaves, flowers, stems, fruits, seeds and roots, or outposts.

In addition, the term "improvement" herein includes not only the prevention or treatment of symptoms, but also the reduction of the range of symptoms, the delay or alleviation of symptoms, the reduction or stabilization of symptoms, partial or complete recovery, and other beneficial results. It is used to mean, and in particular, to improve the condition of hair and scalp. More specifically, the improvement of the hair and scalp condition may preferably be prevention or treatment of hair loss (promoting hair growth), and may also be preferably ^' reducing sebum secretion, preventing dandruff, or preventing scalp inflammation.

Composition for improving hair and scalp condition _, water according to a preferred embodiment of the present invention comprises at least one extract selected from 16 kinds of medicinal herbs as an active ingredient.

In upper limb medicine is the nogeun, peony, duplex, seokwi, Jenny, senna leaves, jadanhyang, excess, Alismataceae, spectators, flaxseed, Pueraria, turmeric, parenthesis ruin, maan and Joiner, described in more detail with respect to the ^"medicine Is as follows.

First of all, Phragmites coiwmmis, a reed root stem, is effective for febrile diseases, and is caused by waste heat, pulmonary tuberculosis, and lung abscess. ), Is a medicine for treating vomiting due to stomach fever, and peony 藥， Paeon iae Radix, promotes digestion and absorption by promoting digestive tract motor activity, pain relief, cramping, sedation and stomach acid It has the effect of suppressing secretion, and various varieties such as Earl Peony, Red Peony, Pear Pear, and Peony can be used. Bukshin is a white part that naturally has a pine root in the middle of the ghost of a holey mushroom (¾r / a cocos), and it calms the mind and insomnias and forgetfulness. It is used as a medicinal herb. Seok-seok (石筆, Pyrrosia 7/7 ^ / a (Thunberg) Farwell or cognate plants) is used as a medicinal herb because it has antitussive, expectorant, and relaxing effects on the front and root. Also. Gonorrhea, urethritis, cystitis, nephritis, etc. as a genitourinary disease. Urethral stones are also used in combination with other drugs. It is recognized as effective for chronic bronchitis and has anti-inflammatory and diuretic effects in acute nephritis and pyelonephritis.

Jenny (養 ^ ， Adenophora re oti flora (Siebold & Zucc.) Miq.) Is a herb used for detoxification, boil treatment, expectoration, etc. Bunsa leaf (番 ^ 葉), 5e / 33 / ¾72ίΓ / Λ3) has a dead action, antibacterial, antibiotic and total poisoning action.

Rosewood is a rosewood (Pterocarpus santalinus / ye (soybean and Legumnosae) stem, and it has the effect of bleeding, hemostasis, detoxification, swelling, and pain). It is used for internal or external use in bleeding or carbuncle, and excess (Agro tsao-ko Crevst et Lemaire) is good for relieving feelings, warming the inside, vomiting and abdominal pain. It relieves only the window, and it has the effect of removing the wall, and is also used for abdominal pain, bloating due to soaking, vomiting due to stomach cancer, and shalsa.

Taxa (ササ潟， Al isma ^' cana 1 icu.latum) is used as a medicinal herb by drying tubers. It absorbs moisture from the body, lowers blood sugar and blood pressure, and is effective for diabetes. It removes cholesterol and improves blood flow. It is used for symptoms such as nephritis, cystitis, urethritis, diabetes, high blood pressure and fatty liver.

Dryopt er is crassirhizoma NAKAI is a plant belonging to the fern-like cotton family, and the root diameter of the spectators is known as filniaron and phloroglucinol. The pharmacological effect is known as a worm, a cold treatment, etc.

Linseed (亞産仁) belongs to the linaceae (iLjnum Lisitatissimuw Linne) ^ \ ripe seeds, used as a decoction for the treatment of diabetes and intestinal diseases and catarrhal diseases of the bronchus. Brown flower

Thunberginia is mainly used for buds that are not fully bloomed, and are used for headache, fever, thirst, laxity, bloating, and vomiting caused by excessive drinking.

Turmeric (畫黃， aircuma ionga) is a dried root of perennial herb belonging to Gingeraceae, releasing fish blood, smoothing the flow of Gi, and menstruation It does not come out even after a period of time to smoothly pass the pain is the medicine that is effective.

Also, Gulu-in (括樓仁) ^. Night Flies (7/5 · 7 Λ ^ kirilowii Maximowicz) or Yellow Sky TrichosantheskirilowiiMax. var, japonica Kita 画 ra) ^ \ Seeds are effective, such as fever, seawater, constipation, boil, milk secretion. In particular, the sputum with febrile ^bronchitis, when it is not easily spit out, and the beam a closed and gently hold down eliminates group, there is an effect geupseongga bronchiolitis, pleurisy, pneumonia, or the like.

Mine (麻仁) is a hemp ((ab / ss ^ i / ra and seed), rich in fat and glossy, soothing dry matter, helping to communicate smoothly to the intestine and smoothing blood circulation. It is also used as a pain reliever for hard acid, pain, and so on.

Joinery ('蘇. 木, ^' Caesalpinia sappan L.) is a plant belonging to the species L egum i no s ae and is called firewood, fire, red-eye, red, etc. It is not native to Korea, and it is distributed in Zhonggu, Fujian, Guangdong, Guangxi, Guizhou, Yunnan, Hainan Island, and Taiwan. The inner part of the trunk and coarse branches of the lumberjack is called the lumberjack (蘇木), and it is known to have an effect on the heart and blood vessels, central inhibitory action, antibacterial action, and hypoglycemic effect. .

According to one embodiment of the present invention, the composition for improving hair and head mass is

5—Testosterone 5a—reductase Including one or more extracts of the above 16 medicinal herbs with excellent inhibitory activity as an active ingredient, hair and scalp scouring: the appearance can be remarkably improved. Preferably, it may include extracts of root roots, peony, fuxin, stone, main and joiner.

On the other hand, the extract of the nogeun, peony, duplex, Pueraria and extract the joiner may be preferably included in the form of a powder ^", seokwi, Jenny, senna leaves, jadanhyang, excess, Alismataceae, spectators, linseed, and turmeric extract Preferably, the extract may be included in the form of extract, the extract of gyruluin may be included in the oil form, and the extract of the mine may be included in the dilute liquid form.

The composition for improving the state of hair and scalp is typically Depending on the method used, it may be applied as a pharmaceutical composition, a functional food composition, or a hair composition, further including a suitable carrier, excipient, diluent, and the like.

When provided in a pharmaceutical composition, the additive composition is orally prepared according to conventional methods. Or it may be administered parenterally, for example, the oral administration may be used in formulations such as powders, granules, tablets, suspension, emulsion eu, ^"syrup.

Preferred dosages may be amounts suitable for the treatment or prophylaxis of the subject and / or disease, including the age, sex, general health and weight of the subject, type and severity of the disease, type of formulation, composition It may be adjusted according to various factors, including the type and content of other ingredients, the ratio of the composition, the route of administration and duration, etc., and may be appropriately selected by those skilled in the art. The subject may be a mammal, including a human.

When the composition is provided in the form of a hair composition, preferably the composition for improving the condition of the hair and scalp. The water may be added in an amount of 0.0001-99.9% by weight, more preferably 0.1 50% by weight, based on the total weight of the hair composition. Preferably, it may be provided in a formulation selected from the group consisting of hair toner, hair lotion, hair cream, hair spray, hair mousse, hair gel, hair soap, hair shampoo, hair rinse, hair pack, and hair treatment.

In another preferred embodiment of the present invention, the method comprising administering at least one extract of one of the 16 medicines excellent in 5-osterone 5a-reductase inhibitory activity to an individual having hair loss, and A method for improving scalp condition is provided.

The method comprising the "administration" is not intended to oral or parenteral administration can be made by, for parenteral administration, but when to select a skin external use, hypodermic injection, transdermal administration and topical ^{administration,} such as, limited. Preferably, it is used for external skin, and the method of apply | coating to skin can be used.

. For topical administration, it can be administered using semisolid preparations such as ointments, creams or gels. For transdermal administration, semisolid products (aqueous alcoholic gels, etc.) as well as matrix or reservoir patches ) May also be used, but is not limited thereto.

According to another preferred embodiment of the present invention the hair and scalp Provided is a method for preparing a composition for improving condition.

The manufacturing method is preferably at least one selected from the group consisting of root root, peony, fuxin, stone, jenny, senna leaf, rosewood, excess, taxa, spectators, linseed, brownish, turmeric, walnut, main and joiner. Extracting with water, ethanol, or a mixed solvent thereof; Filtering the extract obtained through the extraction step under reduced pressure; And concentration under reduced pressure of the filtrate obtained through the filtration step.

Each of the medicinal herbs may be subjected to a crushing process preferably prior to the extraction step in order to increase the extraction efficiency, in particular, having the form of fruit or seed among the medicinal herbs after cutting using a tool such as scissors It can be used by grinding. The grinding method is not particularly limited. For example, it can be made using various tools such as a blender. ^I Preferably, the. The extraction step may be performed by extracting each medicine separately or by mixing all the medicines in a mixed state. When extracting and using each medicine separately, the extraction solvents may be the same or different from each other.

Also preferably, the extraction step may be carried out by mixing the respective medicines or mixtures thereof with the extraction solvent and leaching them.

The leaching is preferably in the temperature range of 30 to 40 ^° C, more preferably at a temperature of 33 to 38 ^° C., preferably 1 to 3 days, more preferably 45 to 50 hours.

The extraction solvent is not particularly limited, but may preferably be extracted using water, ethanol or a mixed solvent thereof, more preferably. Ethane may be used most preferably 60 to 80% (v / v) of ethanol.

Also preferably the extraction solvent may be used in 400 to 800ml per 100g of medicine. _. .

The vacuum filtration step is not particularly limited and may be accomplished using any method known in the art. _^

Obtained through the filtration step. The filtrate may preferably be further subjected to concentration under reduced pressure. The vacuum concentration step is not particularly limited It may be made using a method well known in the art, and may optionally include a step of freeze drying after the step.

EMBODIMENT OF THE INVENTION Hereafter, an Example demonstrates this invention in detail. The following examples are not limited by the following examples this may make the present invention to illustrate the ^invention, Example 1: Preparation of Extract

Ungeun, Peony, Bok-shin, Seok-be, Jenny, Senna Leaf, Rosewood, Excess, Taxi, Crowd, Flax Seed, Brown Flower, Turmeric. Gluluin, mein, and joiner were prepared and crushed, respectively. Fruits or seeds were cut with scissors and crushed.

1000 ml of bovine (Duran) was mixed with 100 g of the ground medicinal herbs and 600 ml of 70% (v / v) ethanol (EtOH), followed by leaching at 35 ^° C. for 48 hours in a constant temperature water bath.

The obtained leachate was filtered under reduced pressure using a filter paper (Watman No. 21 filter), and the recovered filtrate was concentrated with a reduced pressure concentrator to obtain a crude drug extract as shown in Table 1 below. collection). The prepared extract was dispensed into 50nil leucine, sealed with Parafilm, and stored for 8 hours.

TABLE 1

Experimental Example 1. Quantitative analysis of testosterone and DHT using GC-MS

Chromatograms for quantitative analysis of testosterone and dihydrotertosterone (DHT) in the liver microsomal fraction extracted after the reaction are shown in FIGS. 1 to 4. Experimental Example 2. 5-alpha-reductase inhibitory efficacy evaluation measurement

In order to evaluate the 5-alpha-reductase inhibitory effect of the extract obtained in Example 1, first, the microsomal fraction extraction conditions were established using finasteride (finasteridfeKMerCk) as a control drug. Specifically, the finasteride homogenizer (Homogenizer), tissue homogenize kit. Each fraction was obtained by TissueLyzer ^'to obtain a fraction, which was compared with the target to obtain the results of Table 2 below.

TABLE 2

As shown in Table 2, the efficacy of 5-alpha-reductase was confirmed in all three conditions for extracting the microsomal fraction, but when TissueLyzer was used, the value of 5-alpha-reductase was relatively low. It was found that the activity of 5-alpha-reductase was suppressed.

Repetition of the fraction by Homogenizer was repeated twice to confirm the reproducibility of the 5-alpha reductase inhibitory efficacy evaluation as shown in Table 3 below.

TABLE 3

Experimental Example 3. Evaluation of 5-alpha Reductase Inhibitory Effect of Natural Extracts

In order to evaluate the 5-alpha-reductase inhibitory effect of the natural extract obtained in Example 1, each natural extract and testosterone (testosterone) were dissolved in DMS0 and MeOH, respectively.

First, in order to determine the effects on the two organic solvents, 100 Mg / mL testosterone solution was diluted in DMS0 and MeOH at a concentration of 10 pg / mL, respectively, and the results are shown in Table 4 below.

TABLE 4

As shown in Table 4, it was possible to confirm the efficacy of 5-alpha-reductase in both solvents diluted with testosterone, and it was confirmed that there was no effect by the organic solvent in view of the similar result. .

16 natural extracts obtained in accordance with Example 1 and the lean eye meat extract, red soybean extract, and sanzoin extract obtained by extracting in the same manner as in Example 1 as a control and utilizing the efficacy analysis method of the constructed 5-alpha reductase Inhibitory effect of the 5-alpha reductase was repeated twice using the results shown in Table 5 below.

TABLE 5

As shown in Table 5 above. Extracts of 16 kinds of medicinal herbs obtained according to Example 1 of the present invention did not show any efficacy of 5-alpha-reductase, and thus it was confirmed that the inhibitory effect of 5-alpha-reductase was excellent, while the control eye extract, red soybean extract, And Sanjoin extract was confirmed that there is no inhibitory effect of 5-alpha reductase regardless of the concentration used. Especially seen All 16 natural extracts of the invention were found to be superior to the inhibitory effect of 5-alpha-reductase, as in the conventionally known positive control (goose ginseng). . Experimental Example 4. The present invention compositions using a mouse the hair growth promoting effect Measurement - In order to examine the effect of for the present ^invention, in accordance with hair loss prevention and hair growth promoting compositions, using the C57BL / 6N Mauro ^ Promoting hair growth composition of the present The effect was confirmed. As the composition for preventing hair loss and promoting hair growth, a mine extract and a joiner extract were used. C57BL / 6N and then the mouse is completely epilating the hairs in the second catagen (catagen) aged 7 primary mouse entering a ^"group, to the composition and control substance of the present invention, the mouse 10 rats 150 ^ by continuously once a day 5 weeks Applied. Comparing the degree of hair growth with the naked eye over time ^' conclusion was drawn (Fig. 5). Negative control group used 80% cetanol which had no hair growth promoting effect, and positive control group used 3% minoxidil. Table 6 below is a table comparing the growth of hair over time through visual observation.

TABLE 6

In Table 6,-means that the hair is almost no growth, + means that the hair grows over time, the more the number of +, the more hair is grown. It can be seen that the hair grows in the main extract and the joiner extract, which means that the composition according to the present invention promotes hair growth. ^· Experimental Example 5. The hair growth area measurements

Measuring the ratio of hair growth to hair removal area of the hair growth measurement photograph obtained through Experimental Example 4. For this purpose, i-Solution Lite, an image analysis program, was used. First, the total area of hair removal was measured, and the hair growth rate was measured, and then the hair growth rate was measured as a percentage (%) of the total area. Table 7 shows the growth rate of the hair to the hair removal area over time.

TABLE 7 _.

The hair growth rate was higher in the order of positive control (3% minoxidil), HD-23 (mine extract), HD-124 (pine extract) showed a noticeable increase in hair growth after 5 weeks.

Claims

[Range of request]

[Claim 1]

Root, Peony, Fuxin, Stone, Jenny, Senna Leaf, Rosewood, Excess, Taxi, Spectator ^' ,

. Linseed, Pueraria, turmeric, parenthesis ruin, the hair and ^scalp, improving composition comprising one or five or more extracts selected from the group consisting of ME and joiner as an active ingredient.

^'[Claim ^2]

^According to claim 1 _. , The composition comprising a root extract, peony extract, Fuxin extract, stone extract, mine extract and joiner _ᅵ extract.

[Claim 3]

The composition of claim 1, wherein the composition is for the prevention or treatment of hair loss. 5 [claim 4]

The composition of claim 1, wherein the additive composition is for reducing sebum secretion, preventing dandruff, or preventing scalp inflammation.

[Claim 5]

0 Hair composition comprising the composition according to any one of claims 1 to 4. ^'

[Claim 6]

6. The composition of claim 5, wherein the hair composition comprises a hair toner, a hair lotion, a hair cream. The composition is provided in the formulation selected from the group consisting of hairspray, hair mousse, hazel, hair soap, hair shampoo, hair rinse, hair pack, and hair treatment.

[Claim 7]

One species selected from the group delayed by Root, Peony, Fuxin, Stone, Jenny, Senna Leaf, Rosewood, Excess, Taxi, Spectator, 0 Flax Seed, Brown Flower, Turmeric, Gluluin, Mine, and Joiner. More than water ， ethanol. Or extracting them with a mixed solvent;

. A method for improving the hair and scalp condition according to any one of claims 1 to 4, comprising the step of concentrating the filtrate obtained through an additional filtration step under reduced pressure. ^'

【Claim 8】 _. . ^' -In paragraph 7,

The extracting step is to extract euneun, peony, fuxin, stone, main and joiner, individually or in the form of a mixture, mixed with ethane. Manufacturing method. ^'

[Claim 9]

The method of claim 8,

The extraction is done for 1 to 3 days in a temperature of 30 to 40 ^° C. Manufacturing method