KR20140015199A - Composition for improving condition of hair and scalp comprising natural extracts - Google Patents

Abstract

The present invention relates to a composition for improving the condition of hair and scalp containing natural extracts as active ingredients, a production method thereof, and a method of improving the condition of hair and scalp by injecting the natural extracts. The composition for improving the condition of hair and scalp of the present invention contains the natural extracts as active ingredients, does not cause side effects or toxicity, and can be cost-efficiently applied to hair and scalp by not requiring a medical treatment.

Description

Composition for improving hair and scalp condition comprising natural extract as an active ingredient and a method for manufacturing the same {COMPOSITION FOR IMPROVING CONDITION OF HAIR AND SCALP COMPRISING NATURAL EXTRACTS}

The present invention relates to a composition for improving the condition of hair and scalp comprising a natural extract as an active ingredient and a method for producing the same, and also to a method for improving the condition of hair and scalp by administering the natural extract of the present invention.

Male alopecia, the most common type of alopecia, is converted from testosterone, one of the male hormones, to dihydrotestosterone (DHT), an active male hormone by testosterone 5a-reductase. Activated dehydrotestosterone binds to androgen receptors to delay the hair follicle cell protein synthesis, shortening hair follicle growth and contracting hair follicles, causing hair loss. In addition, during androgenetic alopecia, sebum is excessively produced, and as a result, scalp may be caused by inflammation [Dennis A. Holt, et. al, J Med. Chem., 33, 943, (1990)].

A common treatment for hair loss is hair transplantation. That is, a method of implanting a plug of skin with hair from the scalp where the hair grows to the hair loss site. This method is time consuming, relatively painless, and expensive. Other non-drug therapies include ultraviolet radiation and exercise therapy.

Another treatment for hair loss is drug therapy. Merck of the United States inhibits the action of 5-alpha-reductase and inhibits the production of dihydrotestosterone (DHT), a commercially available hair regrowth made by finasteride for oral administration. Doing. However, there is a problem that side effects occur due to steroidal drugs.

In addition, equol, a major metabolite of diedzein, a type of phytoestrogen, binds directly to dihydrotertosterone (DHT) without affecting testosterone, di It has been reported that it blocks the pathway of hydrotertosterone (DHT).

Therefore, there is a need for developing a therapeutic agent derived from natural products that is economical and does not have various side effects or toxicity concerns.

In order to solve the problems of the prior art, it is an object of the present invention to provide a composition for improving hair and scalp state and a method for producing the same, including a natural extract as an active ingredient.

In addition, the present invention hair loss of one or more extracts selected from the group consisting of Root, Peony, Bokshin, Seok, Jenny, Senna Leaf, Rosewood, Excess, Taxa, Spectrum, Flax Seed, Brown, Turmeric, Gluluin, Mine, and Joiner It is an object of the present invention to provide a method for improving hair and scalp conditions, comprising administering to a subject having a.

In order to achieve the above object, a preferred embodiment of the present invention, Root, Peony, Bokshin, Seok, Jenny, Senna Leaf, Rosewood, Excess, Taxa, Spectator, Flax Seed, Brown, Turmeric, Gluluin, Mine and It provides a composition for improving hair and scalp conditions comprising at least one extract selected from the group consisting of joiner as an active ingredient.

In addition, another preferred embodiment of the present invention provides a hair composition comprising the composition.

In another preferred embodiment of the present invention, in the group consisting of Root, Peony, Bokshin, Seok, Jenny, Senna Leaf, Rosewood, Excess, Taxa, Crowd, Flax Seed, Brownish, Turmeric, Gluluin, Mine and Joiner Provided is a method for improving hair and scalp conditions, comprising administering at least one extract selected to a subject having hair loss.

In another preferred embodiment of the present invention, in the group consisting of Root, Peony, Bokshin, Seok, Jenny, Senna Leaf, Rosewood, Excess, Taxa, Crowd, Flax Seed, Brownish, Turmeric, Gluluin, Mine and Joiner Extracting at least one selected from water, ethanol, or a mixed solvent thereof;

Filtering the extract obtained through the extraction step under reduced pressure; And

It provides a method for producing a composition for improving the hair and scalp conditions comprising the step of concentrating the filtrate obtained through the filtration step under reduced pressure.

Hereinafter, the present invention will be described in more detail.

Testosterone 5α-reductase is a testosterone, one of the male hormones, and dihydrotestosterone, the active male hormone.

testosterone (DHT), and this activated dehydrotestosterone binds to androgen receptors, which slows down the hair follicle growth and shortens hair follicle growth and causes hair loss. In addition, during androgenetic alopecia, sebum is excessively produced, and as a result, scalp may be caused by inflammation [Dennis A. Holt, et. al, J Med. Chem., 33, 943, (1990)].

Therefore, by inhibiting the 5-alpha-reductase, hair loss can be prevented and hair growth can be promoted, and sebum secretion and dandruff can be prevented or reduced, and scalp inflammation can also be prevented or improved (Olsen EA, Hordinsky M). , Whiting D, et al "The importance of dual 5alpha-reductase inhibition in the treatment of MPB: results of a randomized placebo-controlled study of dutasteride versus finasteride". J Am Acad Dermatol . 55 (6), 2006, Leyden, James; Dunlap, Frank; Miller, Bruce; Winters, Peter; Lebwohl, Mark; Hecker, David; Kraus, Stephen; Baldwin, Hilary et al. "Finasteride in the treatment of men with frontal male pattern hair loss". Journal of the American Academy of Dermatology 40 (6 Pt 1): 930-7., 1999).

The present inventors have developed a new type of natural product-derived formulation having no side effects or toxicity, and having an excellent effect on improving hair and scalp conditions, which can replace existing drugs frequently causing unwanted side effects with respect to hair and scalp conditions. As a result of the study, the extracts and specific fractions of certain medicinal herbs were confirmed to be remarkably superior to the 5-alpha-reductase (testosterone 5α-reductase) inhibitory effect was completed the present invention.

As used herein, the term "extract" refers to an extract obtained from various organs of each medicine, such as leaves, flowers, stems, fruits, seeds and roots, or outposts.

In addition, the term "improvement" herein includes not only the prevention or treatment of symptoms, but also the reduction of the range of symptoms, delay or alleviation of symptoms progression, alleviation or stabilization of symptoms, partial or complete recovery, and other beneficial results. It is used to mean, especially means for the improvement of hair and scalp conditions.

More specifically, the improvement of the hair and scalp condition may preferably be prevention or treatment of hair loss (promoting hair growth), and may also preferably reduce sebum secretion, prevent dandruff, or prevent scalp inflammation.

The hair and scalp condition improving composition according to an embodiment of the present invention is 16 kinds At least one extract selected from medicinal herbs is included as an active ingredient.

The medicinal herbs include Root, Peony, Bokshin, Seok, Jenny, Senna Leaf, Rosewood, Excess, Taxa, Spectator, Flax Seed, Brown, Turmeric, Gluluin, Mine, and Joiner. As follows.

First of all, Phragmites communis is the root stem of reeds, which is effective for febrile diseases, and due to waste heat, seawater, pulmonary tuberculosis, lung abscess, and stomach fever. It is a medicine that treats vomiting due to (胃熱), and Peony (e, Paeoniae Radix ) promotes digestive and motility activity, promotes digestion and absorption, and has analgesic, analgesic, sedative and gastric acid secretion inhibitory effects. A variety of varieties are available, including White Peony, Red Peony, Root Peony, and Crop Peony.

Blessed God is Poria with Pooch Mushroom cocos ) A white part that naturally contains pine cones. It is used as a medicine to calm the mind and to treat insomnia, forgetfulness, and so on. Pyrrosia lingua (Thunberg) Farwell (or similar plant) is used as a medicinal herb with darker, expectorant, and slower action on leaves and roots. Also, as a genitourinary disease, it has diuretic and antipyretic effects on symptoms such as gonorrhea, urethritis, cystitis and nephritis, and is used in combination with other drugs for urolithiasis. It is also effective in chronic bronchitis and has anti-inflammatory and diuretic effects in acute nephritis and pyelonephritis.

Jenny ( Adenophora remotiflora (Siebold & Zucc.) Miq .) decrypts the heat down to the roots and the wall (there is an effect sakyineun痰) is a medicine used for detoxification, boils treatment, geodam, senna leaves (leaf beonsa (番瀉葉), Senna alexandrina ) acts as a hypothalamic, antimicrobial, antibiotic and toxicant.

Rosewood incense ( Pterocarpus santalinus Linne (soybean and Leguminosae ) is the main stem, and it has the effect of cold blood, hemostasis, detoxification, small bowel, and pain, sores and wounds. Used for underwear or external use in 腫 瘡毒), excess (草 果, Amomum tsao - ko Crevst meat Lemaire ) relieves chills, warms the inside, relieves vomiting, and relieves only the abdominal window. In addition, it has the effect of removing the phlegm, and is also applied to abdominal pain, bloating due to soaking, vomiting due to stomach cancer, diarrhea.

Alixama canaliculatum is a medicinal herb that dries the roots and absorbs moisture from the body, lowers blood sugar and blood pressure, and is effective for diabetes, and removes waste and cholesterol from the blood. Efficacy in making it good for nephritis, cystitis, urethritis, diabetes, high blood pressure, fatty liver and other symptoms.

The crowd (貫衆, Dryopteris crassirhizoma NAKAI ) is a plant belonging to the ferns of the horses, the roots of the crowd are known as filmaron, phloroglucinol, etc., and the pharmacological effect is known as a worm, a cold treatment.

Flax (亞麻仁) is Flax (Linum belonging to ahmagwa (Linaceae) ripe seeds of usitatissimum Linne ), used as a coolant in the treatment of diabetes and intestinal diseases and catarrhal diseases of the bronchus, Pueraria thunberginia ) mainly uses buds that have not fully bloomed and are used for headache, fever, thirst, anorexia, bloating, and vomiting due to heavy drinking.

Curcuma longa is a dried perennial herb that belongs to Gingeraceae, releasing fish blood, smoothing the flow of Gi, and allowing the menstrual period to pass smoothly. It is an effective medicine to stop.

In addition, the Zhu Ruin is also known as the Trichosanthes kirilowii Maximowicz ) or Yellow- crowned Star ( Trichosanthes) kirilowii Max . there is . japonica Kitamura ) Seeds, and has the effect of fever, seaweed, constipation, boils, milk secretion. Especially when sputum does not spit well due to febrile bronchitis, the lungs are softened, the GI lowers the wall, and it is effective in acute bronchitis, pleurisy and pneumonia.

Mine is Cannabis sativa ) Seeds are rich in fat and shine, it helps to dry dry, soften the intestines, and improve blood circulation. It is also used as a pain reliever for hard acid and pain.

Joiner, Caesalpinia sappan L. is a plant belonging to Leguminosae and is called Firewood, Fire, Red-eye, Red, etc., and is not native to Korea. It is distributed in provinces, Guangdong province, Guangxi province, Guizhou province, Yunnan province, Hainan Island and Taiwan. The inner part of the stem and coarse branches of the lumberjack is called the lumberjack (蘇木), and it is known that the action on the heart and blood vessels, central inhibitory action, antibacterial action, and blood sugar lowering action.

The composition for improving hair and scalp condition according to an embodiment of the present invention comprises hair and scalp by including one or more extracts of the 16 medicinal herbs excellent in 5-alpha-reductase inhibitory activity as an active ingredient. The condition can be significantly improved. Preferably, it may include extracts of root roots, peony, fuxin, laxatives, mine and joiner.

On the other hand, the extract of the root root, peony, fuxin, browning and joiner of the extract may preferably be included in powder form, and the extracts of Seok, Jenny, Senna Leaf, Rosewood, excess, Taxa, spectators, linseed, and turmeric are preferred. Preferably it may be included in the form of extract, the extract of the parenthein may be included in the oil form, the extract of the mine may also be included in a dilute liquid form.

The hair and scalp condition improving composition may be applied as a pharmaceutical composition, a functional food composition, or a hair composition further including a suitable carrier, excipient and diluent, etc. according to methods commonly used in each field.

When provided as a pharmaceutical composition, the composition may be administered orally or parenterally according to a conventional method, for example, in the case of oral administration may be used in the form of powders, granules, tablets, suspensions, emulsions, syrups and the like.

The preferred dosage amount may be a content suitable for the treatment or prevention of the subject and / or disease, including the age, sex, general health and weight of the subject, the kind and severity of the disease, the type of formulation, The type and content of the other components, the proportion of the composition, the route and the duration of administration, and the like, and may be appropriately selected by those skilled in the art. The subject may be a mammal, including a human.

When the composition is provided in the form of a hair composition, preferably the composition for improving the hair and scalp condition may comprise 0.0001 to 99.9% by weight, more preferably 0.1 to 50% by weight based on the total weight of the hair composition. , Preferably hair toner, hair lotion, hair cream, hair spray, hair mousse, hair gel, hair soap, hair shampoo, hair rinse, hair pack, and hair treatment.

In another preferred embodiment of the present invention, the method comprising administering at least one extract of one of the 16 medicines excellent in 5-osterone 5α-reductase inhibitory activity to a subject with hair loss, and A method for improving scalp condition is provided.

The step of 'administering' may be made orally or parenterally, and the parenteral administration may be selected from external skin, subcutaneous injection, transdermal and topical administration, but is not limited thereto. Preferably, it is used for external use of the skin, and the method of apply | coating to skin can be used.

For topical administration, it can be administered using semisolid preparations such as ointments, creams or gels. For transdermal administration, semisolid products (aqueous alcoholic gels, etc.) can be used as well as matrix or reservoir patches. ) May also be used, but is not limited thereto.

According to another preferred embodiment of the present invention there is provided a method for producing the composition for improving the hair and scalp conditions.

The production method is preferably at least one selected from the group consisting of root root, peony, fuxin, stone, jenny, senna leaf, rosewood, excess, taxa, spectator, linseed, brownish, turmeric, walnut, mine and joiner Extracting with water, ethanol, or a mixed solvent thereof; Filtering the extract obtained through the extraction step under reduced pressure; And concentration under reduced pressure of the filtrate obtained through the filtration step.

Each of the medicinal herbs may be subjected to a crushing process preferably prior to the extraction step in order to increase the extraction efficiency, in particular, having the form of fruit or seed among the medicinal herbs after cutting using a tool such as scissors It can be used by grinding. The grinding method is not particularly limited and may be made using various tools such as a mixer.

Preferably, the extracting step may be performed by extracting each medicine separately or by mixing all the medicines in a mixed state. When extracting and using each medicine separately, the extraction solvents may be the same or different from each other. .

In addition, the extraction step may be made of a method of leaching after mixing each medicine or a mixture thereof with the extraction solvent.

The leaching may be preferably performed at a temperature in the range of 30 to 40 ° C., more preferably at a temperature of 33 to 38 ° C., preferably 1 to 3 days, and more preferably 45 to 50 hours.

The extraction solvent is not particularly limited, but may be preferably extracted using water, ethanol or a mixed solvent thereof, more preferably ethanol, and most preferably 60 to 80% (v / v) ethanol. have.

Also preferably the extraction solvent may be used in 400 to 800ml per 100g of medicine.

The vacuum filtration step is not particularly limited and may be performed using methods well known in the art.

The filtrate obtained through the filtration step may preferably be further subjected to a concentration under reduced pressure. The concentration under reduced pressure is not particularly limited and may be performed using a method well known in the art, and may also optionally include a step of freeze drying after the step.

According to the present invention, a composition for improving hair and scalp condition comprising a natural extract having an excellent inhibitory effect on 5-alpha-reductase (testosterone 5α-reductase) as an active ingredient, a method for preparing the same, and a natural extract is administered to the hair and scalp condition. A method of improving is provided. The hair and scalp condition improving composition contains a natural extract as an active ingredient, there is no fear of side effects or toxicity, and does not require a procedure can be economically and easily applied.

1 is obtained in accordance with Experiment 1 of the present invention Extracted-ion chromatograms of d ₃ -testosterone ((a): Reference standard, (b): Microsomal fraction).
2 is Extracted-ion chromatograms of testosterone obtained according to Experimental Example 1 of the present invention ((a): Reference standard, (b): Microsomal fraction).
Figure 3 is a-Extracted ion chromatograms of d ₃ -dihydrotestosterone (d ₃ -DHT) obtained according to Experimental Example 1 of the present invention ((a): Reference standard, (b): Microsomal fraction).
4 is Extracted-ion chromatograms of dihydrotestosterone (DHT) obtained according to Experimental Example 1 of the present invention ((a): Reference standard, (b): Microsomal fraction).
Figure 5 shows the growth of hair over time obtained according to Experimental Example 4 of the present invention.

Hereinafter, the present invention will be described in more detail with reference to Examples. However, these examples are only for illustrating the present invention, and the scope of the present invention is not limited by these examples.

Example  1. Preparation of Extract

Root, Peony, Bokshin, Seokwi, Jenny, Senna Leaf, Rosewood, Excess, Taxa, Spectator, Flax Seed, Brownish, Turmeric, Gluluin, Mine and Joiner were prepared and crushed. .

100 g of the pulverized medicinal herb and 600 ml of 70% (v / v) ethanol (EtOH) were added to a 1000 ml bottle (Duran), followed by mixing and leaching at 35 ° C. for 48 hours in a constant temperature water bath.

The obtained leachate was filtered under reduced pressure using a filter paper (Watman No. 21 filter), and the recovered filtrate was concentrated with a reduced pressure concentrator to obtain a crude drug extract as shown in Table 1 below. collection). The prepared extracts were dispensed into 50ml tubes, sealed with Parafilm, and refrigerated.

A good name Gain (g) Yield (%) Recovery form Labor 0.22 0.22 powder Peony 2.43 2.43 powder Double 0.26 0.26 powder Above 0.93 0.93 Extract Jenny 0.16 0.16 Extract Senna leaves 1.24 1.24 Extract Self-direction 1.21 1.21 Extract Excess 1.32 1.32 Extract Tarsa 0.93 0.93 Extract crowd One One Extract Flax 0.52 0.52 Extract Gall 3.65 3.65 powder curcuma 15 15 Extract Gluluin 1.86 1.86 oil Joino 2.78 2.78 powder Mine 8.83 8.83 Dilute liquid

Experimental Example  One. GC - MS Testosterone using testosterone )and DHT Quantitative analysis of

Chromatograms for quantitative analysis of testosterone and dihydrotertosterone (DHT) in the liver microsomal fraction extracted after the reaction are shown in FIGS. 1 to 4.

Experimental Example  2. 5- Alpha Reductase  Inhibitory efficacy assessment

In order to evaluate the inhibitory effect of 5-alpha-reductase of the extract obtained in Example 1, first, microsomal fraction extraction conditions were established using finasteride (Merck) as a control drug.

Specifically, the finasteride was fractionated by a homogenizer, a tissue homogenize kit, and a TissueLyzer, respectively, to obtain a fraction. The results of Table 2 were obtained by comparing the results.

Finasteride (mM) Concentration (ng / mL) Efficacy of 5-alpha Reductase (DHT / T) Testosterone DHT Homogenizer 0 54.16 5.70 0.105 0.1 189.01 - - Tissue 0 85.54 10.57 0.124 homogenize kit 0.1 176.62 - - TissueLyzer 0 134.63 4.05 0.030 0.1 182.49 - -

As shown in Table 2, the efficacy of 5-alpha-reductase was confirmed in all three conditions for extracting the microsomal fraction, but when TissueLyzer was used, the value of 5-alpha-reductase was relatively low. It was found that the activity of 5-alpha-reductase was suppressed.

Repetition of the fraction by Homogenizer was repeated twice to confirm the reproducibility of the 5-alpha reductase inhibitory efficacy evaluation as shown in Table 3 below.

No. Finasteride (mM) Concentration (ng / mL) Efficacy of 5-alpha Reductase (DHT / T) Testosterone DHT One 0 63.09 5.70 0.090 One 0.1 142.72 - - 2 0 21.03 2.34 0.111 2 0.1 127.24 - -

Experimental Example  3. 5- of natural extract Alpha Reductase  Inhibitory efficacy assessment

In order to evaluate the 5-alpha-reductase inhibitory effect of the natural extract obtained in Example 1, each natural extract and testosterone (testosterone) were dissolved in DMSO and MeOH, respectively.

First, in order to determine the effects on two organic solvents, 100 μg / mL testosterone solution was diluted and diluted at a concentration of 10 μg / mL in DMSO and MeOH, respectively, and the results are shown in Table 4 below.

Concentration (ng / mL) Efficacy of 5-alpha Reductase (DHT / T) Testosterone DHT Testosterone 46.17 2.01 0.044 (in DMSO) 42.78 2.92 0.068 Testosterone 45.63 2.24 0.049 (in MeOH) 42.16 1.70 0.040

As shown in Table 4, it was possible to confirm the efficacy of 5-alpha-reductase in both solvents diluted with testosterone, and in view of the similar result, it was confirmed that there was no effect by the organic solvent.

Utilizing the method for analyzing the efficacy of the constructed 5-alpha reductase, 16 kinds of natural extracts obtained in accordance with Example 1 and the extracts of longeye meat extract, red soybean extract, and sanjoin extract obtained by the same method as Example 1 as a control. Inhibitory effect of the 5-alpha reductase was repeated twice using the results shown in Table 5 below.

Name density Concentration (ng / mL) 5-Alpha Reductase Efficacy
(DHT / T) (μg / mL) Testosterone DHT
HD-021
(Labour) 50 174.21 - - 50 173.79 - - 100 175.74 - - 100 171.18 - -
HD-023
(Mine) 50 33.32 - - 50 27.52 - - 100 29.73 - - 100 30.84 - -
HD-024
(Repeat) 50 172.37 - - 50 168.26 - - 100 169.73 - - 100 177.61 - -
HD-041
(Above) 50 153.00 - - 50 144.94 - - 100 152.86 - - 100 157.36 - -
HD-045
(Jenny) 50 151.83 - - 50 145.61 - - 100 141.67 - - 100 136.10 - -
HD-056
(Senna leaf) 50 148.24 - - 50 155.03 - - 100 159.21 - - 100 161.12 - -
HD-062
(Scented) 50 144.97 - - 50 138.11 - - 100 146.75 - - 100 148.82 - -
HD-069
(Excess) 50 139.29 - - 50 147.74 - - 100 151.29 - - 100 154.45 - -
HD-074
(crowd) 50 148.67 - - 50 164.99 - - 100 159.40 - - 100 152.05 - -
HD-082
(Amine) 50 162.85 - - 50 163.87 - - 100 158.74 - - 100 164.19 - -
HD-088
(Galvanized) 50 167.09 - - 50 167.87 - - 100 162.77 - - 100 151.48 - -
HD-089
(curcuma) 50 156.56 - - 50 166.73 - - 100 158.36 - - 100 157.24 - -
HD-095
(Blind-in) 50 149.58 - - 50 143.89 - - 100 156.40 - - 100 157.28 - -
HD-118
(Peony) 50 147.78 - - 50 157.82 - - 100 159.49 - - 100 144.05 - -
HD-120
(Taxi) 50 122.55 - - 50 160.64 - - 100 129.35 - - 100 146.52 - -
HD-124
(Joint) 50 0.29 - - 50 0.47 - - 100 0.70 - - 100 0.78 - -
HD-058
(Dragon meat) 50 62.39 1.46 0.02 50 56.56 1.64 0.03 100 72.05 2.49 0.04 100 74.51 1.45 0.02
HD-064
(Red bean) 50 71.90 0.77 0.01 50 65.44 1.08 0.02 100 86.58 0.91 0.01 100 75.28 1.35 0.02
HD-078
(Sanjoin) 50 166.84 0.90 0.01 50 162.14 1.02 0.01 100 161.52 1.01 0.01 100 162.74 0.95 0.01
P / C
(Ginseng)
50 352.74 - - 50 344.03 - - 100 335.35 - - 100 211.92 - -

As shown in Table 5, the extract of 16 kinds of medicinal herbs obtained according to Example 1 of the present invention did not show any efficacy of 5-alpha-reductase at all, whereas it was confirmed that the inhibitory effect of 5-alpha-reductase was excellent. It was confirmed that there was no inhibitory effect of 5-alpha-reduced enzyme in irrigation extracts, yongan meat extract, red soybean extract, and sanjoin extract. In particular, all 16 natural extracts of the present invention were found to be superior to the inhibitory effect of 5-alpha-reductase as in the conventionally known positive control (positive control) ginseng.

Experimental Example  4. Measurement of mouse hair growth promoting effect using the composition of the present invention

In order to determine the effect of the composition for preventing hair loss and promoting hair growth according to the present invention, the hair growth promoting effect of the composition was confirmed using C57BL / 6N mice. As the composition for preventing hair loss and promoting hair growth, a mine extract and a joiner extract were used. After completely depilating the hair of the 7-week-old mouse when the C57BL / 6N mouse enters the second catagen phase, 10 μl of the composition and the control material of the present invention were applied once a day for 5 weeks in a row at 150 μl. It was. The conclusion was drawn by visually comparing the degree of hair growth promotion over time (FIG. 5). Negative control group used 80% ethanol without hair growth promoting effect and 3% minoxidil was used as a positive control group. Table 6 below is a table comparing the growth of hair over time through visual observation.

Elapsed days

matter 1 week 3 weeks 5 weeks  Negative control group - - -  Positive control group - +++ ++++ HD-23
Mine Extract - +++ ++++ HD-124
(Joint Extract) - ++ ++++

In Table 6,-means that the hair is almost grown, + means that the hair grows over time, the more the number of +, the more hair is grown. It can be seen that the hair grows in the main extract and the joiner extract, which means that the composition according to the present invention promotes hair growth.

Experimental Example  5. Hair growth area measurement

In order to measure the ratio of the hair growth area to the hair removal area, the hair growth measurement photograph obtained through Experimental Example 4 was used as an image analysis program i-Solution Lite. First, the total area of hair removal was measured, the area of hair growth was measured, and then the hair growth rate was measured as a percentage of the total area. Table 7 shows the growth rate of the hair to the hair removal area over time.

     group
week Negative control group Positive control group HD -23 HD -124 Week 1 0.00 ± 0.00 0.00 ± 0.00 0.00 ± 0.00 0.00 ± 0.00 Week 3 0.00 ± 0.00 95.46 ± 23.42 71.45 ± 1.25 18.62 ± 4.85 Week 5 0.00 ± 0.00 98.72 ± 25.31 95.13 ± 26.41 87.21 ± 22.54

The hair growth rate was higher in the order of positive control (3% minoxidil), HD-23 (mine extract), and HD-124 (pine extract) showed a noticeable increase in hair growth rate after 5 weeks.

Claims (9)

Hair that contains one or more extracts selected from the group consisting of Root, Peony, Bokshin, Seok-wi, Jenny, Senna Leaf, Rosewood, Excess, Taxa, Crowd, Flax Seed, Brownish, Turmeric, Gluluin, Mine, and Joiner as active ingredients And a composition for improving scalp condition.
The composition of claim 1, wherein the composition comprises a root extract, a peony extract, a fuxin extract, a laxative extract, a mine extract and a joiner extract.
The composition of claim 1, wherein the composition is for preventing or treating hair loss.
The composition of claim 1, wherein the composition is for reducing sebum secretion, preventing dandruff, or preventing scalp inflammation.
A composition for hair comprising the composition according to any one of claims 1 to 4.
The formulation of claim 5, wherein the composition for hair is selected from the group consisting of hair toner, hair lotion, hair cream, hair spray, hair mousse, hair gel, hair soap, hair shampoo, hair rinse, hair pack, and hair treatment. It is provided as a composition.
At least one member selected from the group consisting of Root, Peony, Bokshin, Seok, Jenny, Senna Leaf, Rosewood, Excess, Taxa, Crowd, Flax Seed, Brownish, Turmeric, Gluluin, Mine, and Joiner Extracting with a mixed solvent;
Filtering the extract obtained through the extraction step under reduced pressure; And
The method for preparing a composition for improving hair and scalp condition according to any one of claims 1 to 4, comprising the step of concentrating the filtrate obtained through the filtration step under reduced pressure.
The method of claim 7, wherein
The extraction step is to extract the roots, peony, fuxin, stone, mine and joiner, respectively or in the form of a mixture, by mixing with ethanol.
9. The method of claim 8,
The extraction is made for 1 to 3 days at a temperature of 30 to 40 ℃, manufacturing method.

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