WO2013129245A1 - 殺菌剤組成物 - Google Patents
殺菌剤組成物 Download PDFInfo
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- WO2013129245A1 WO2013129245A1 PCT/JP2013/054475 JP2013054475W WO2013129245A1 WO 2013129245 A1 WO2013129245 A1 WO 2013129245A1 JP 2013054475 W JP2013054475 W JP 2013054475W WO 2013129245 A1 WO2013129245 A1 WO 2013129245A1
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- bactericidal
- ultrafine bubbles
- sterilizing
- sterilizing component
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/16—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using chemical substances
- A61L2/18—Liquid substances or solutions comprising solids or dissolved gases
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/34—Shaped forms, e.g. sheets, not provided for in any other sub-group of this main group
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/36—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/12—Iodine, e.g. iodophors; Compounds thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
- A61P1/02—Stomatological preparations, e.g. drugs for caries, aphtae, periodontitis
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/02—Local antiseptics
Definitions
- the present invention relates to a novel bactericidal composition that can be sterilized in the presence of various organic contaminants in the environment or biofilm by using water containing ultrafine bubbles, and a method for producing the same. And a sterilization method using the same.
- Bactericides are widely used in all fields such as industry, cosmetics, food processing, pharmaceuticals, agriculture, and dairy.
- germicides used in the medical and food fields, for example, chlorine germicides, iodine germicides, peroxide germicides, aldehyde germicides, phenol germicides, biguanide germicides.
- Agent mercury fungicide, alcohol fungicide, quaternary ammonium salt fungicide, amphoteric surfactant fungicide and the like.
- many of these bactericides showed a significant decrease in bactericidal activity against soils caused by organic substances such as proteins and biofilms covered with polysaccharides and proteins.
- biofilms are attracting attention because they may cause serious problems in the living environment and industry. For example, in a living environment, the toilets, kitchens, bathrooms, and the like are slimmed, that is, they cause a bad odor and cause discomfort. Infectious diseases may occur due to bacteria in the biofilm formed in the circulating baths of hot spring facilities.
- an industrial problem there is a case where corrosion is caused by the formation of a biofilm on a sewer pipe or the bottom of a ship, and a biofilm on a factory production line may cause microbial contamination.
- biofilms formed on medical devices can be a source of infection, and can also cause disease due to biofilm formation in human tissues such as skin.
- dental plaque plaque
- biofilms formed on teeth so-called dental plaque (plaque)
- caries and periodontal disease.
- fresh food products such as vegetables, raw materials for processed foods, and biofilms formed on cooking utensils cause rot and food poisoning.
- the actual use concentration is defined as a concentration far higher than the sterilization concentration obtained in the experimental system.
- Non-patent Documents 1, 2, and 3 A combination of anionic surfactants has been proposed as a method for improving the permeability to biofilms.
- Patent Documents 1, 2, and 3 A combination of anionic surfactants has been proposed as a method for improving the permeability to biofilms.
- these known techniques have problems. For example, assuming that there is organic dirt in actual use, the concentration is set to a concentration much higher than the sterilization concentration obtained in the experimental system. It was not preferable from the viewpoint of living organisms and environmental safety.
- glycerin fatty acid esters that do not lose their bactericidal and antibacterial activity even when organic matter is contaminated have the disadvantage of not having antibacterial properties against gram-negative bacteria (Non-patent Document 1).
- Patent Documents 4, 5, and 6 ethylenediaminetetraacetic acid, which is a chelating agent having bactericidal activity against gram-negative bacteria, to compensate for the disadvantages of glycerin fatty acid esters.
- Patent Documents 4, 5, and 6 ethylenediaminetetraacetic acid, which is a chelating agent having bactericidal activity against gram-negative bacteria.
- the degree of freedom in blending such as the combination of blending being limited, such as reaction with hypochlorous acid or a salt thereof, reducing the effective chlorine concentration and reducing the bactericidal activity.
- an anionic surfactant has been proposed (Patent Documents 1, 2, and 3).
- There was a problem in the degree of freedom of blending such as the sterilizing power being reduced.
- An object of the present invention is to provide a bactericide composition exhibiting an excellent bactericidal effect even in the presence of organic substances and biofilms, a production method thereof, and a bactericidal method using the same.
- the present invention relates to a bactericidal composition
- a bactericidal composition comprising water containing ultrafine bubbles having a mode particle diameter of 500 nm or less and a bactericidal component.
- the “mode particle density”, which is the number per 1 mL of particles having the mode particle diameter of ultrafine bubbles, is 10,000 or more.
- the “total particle density”, which is the total number of fine bubble particles per mL is 1 million or more.
- the density of ultrafine bubbles having a particle diameter of 1000 nm or less, which is the number per 1 mL of ultrafine bubbles having a particle diameter of 1000 nm or less is preferably 1 million or more.
- the inside of the ultrafine bubbles may be one or more gases selected from air, oxygen, hydrogen, nitrogen, carbon dioxide, argon, neon, xenon, fluorinated gas, ozone, and inert gas. it can.
- the sterilizing component used in the present invention includes iodine sterilizing component, peroxide sterilizing component, aldehyde sterilizing component, phenolic sterilizing component, biguanide sterilizing component, mercury sterilizing component, alcohol sterilizing component, quaternary sterilizing component. It can be an ammonium salt-based bactericidal component, an amphoteric surfactant-based bactericidal component, or a naturally derived bactericidal component.
- the present invention provides a method for producing a bactericide composition, which comprises mixing water containing ultrafine bubbles having a mode particle diameter of 500 nm or less and a bactericidal component.
- this invention provides the manufacturing method of a disinfectant composition including generating the ultrafine bubble whose mode particle diameter is 500 nm or less in the water containing a disinfection component.
- the mode particle density of the ultrafine bubbles is 10,000 or more, and more preferably 100,000 or more.
- the total fine particle density and the density of ultrafine bubbles having a particle diameter of 1000 nm or less are 1 million or more.
- a biofilm refers to a higher-order structure formed by microorganisms, for example, a film formed by being bound by an extracellular polymer compound (EPS) such as a polysaccharide.
- EPS extracellular polymer compound
- biofilms are as described above, such as residential toilets, kitchens, bathrooms, hot tubs and other circulating tubs, various pipes such as sewer pipes, ship bottoms, factory production lines, dialysis tubes, etc.
- Examples include medical devices such as mirrors and contact lenses, human skin, oral cavity, fresh food products such as vegetables, processed food ingredients, and biofilms formed on cooking utensils.
- a bactericidal composition exhibiting an excellent bactericidal effect even in the presence of organic substances and biofilms, and a bactericidal method using the bactericidal composition are obtained.
- the ultrafine bubbles used in the present invention have a mode particle size of 500 nm or less, preferably a mode particle size of 300 nm or less, more preferably a mode particle size of 150 nm or less, and most preferably a mode particle size.
- the mode particle density is preferably 10,000 or more, more preferably 50,000 or more, more preferably 500,000 or more, more preferably 5 million or more, more preferably 10 million or more, Preferably it is 50 million or more, more preferably 100 million or more, more preferably 500 million or more, and most preferably 700 million or more.
- the density of ultrafine bubbles having a particle diameter of 1000 nm or less and the total particle density are preferably 1 million or more, more preferably 4 million or more, more preferably 40 million or more, more preferably 100 million or more, More preferably 400 million or more, more preferably 1 billion or more, more preferably 3 billion or more, more preferably 5 billion or more, more preferably 7 billion or more, more preferably 10 billion or more, more preferably Can be 20 billion or more, most preferably 40 billion or more.
- the “total particle density” and the density of ultrafine bubbles having a particle diameter of 1000 nm or less agree. Since there are almost no bubbles of 1000 nm or more in the examples described later, both are used as terms of consent.
- the particle size of the ultrafine bubbles used in the present invention is so small that it cannot be accurately measured with a normal particle size distribution analyzer. Therefore, in this specification, the numerical value measured by the nano particle analysis system Nanosite series (made by NanoSight) is used. Nanoparticle analysis system Nanosite Series (manufactured by NanoSight) measures the speed of Brownian motion of nanoparticles and calculates the particle diameter from the speed. The mode particle diameter can be confirmed from the particle diameter distribution of the existing particles, and means the particle diameter when the number reaches the maximum value.
- the water used in the present invention is not limited to these, but tap water, purified water, ion exchange water, pure water, ultrapure water, deionized water, distilled water, buffer solution, clean water, natural water It can be selected from water, filtered water, high purity water, drinking water and electrolyzed water.
- a water-soluble solvent such as alcohol, glycol, glycerin, ether, ketone, ester or the like can also be added.
- the zeta potential on the surface of the ultrafine bubbles affects the stability of the bubbles.
- the surface of the ultrafine bubbles used in the present invention is charged, and the absolute value of the zeta potential thereof is 5 mV or more, preferably 7 mV or more, more preferably 10 mV or more, more preferably 20 mV or more, further preferably 25 mV or more, most preferably. 30 mV or more.
- the ultrafine bubbles used in the present invention are generated by any known means, for example, a static mixer type, a venturi type, a cavitation type, a vapor agglomeration type, an ultrasonic type, a swirl type, a pressure dissolution type, or a fine hole type. Can be made.
- a preferred method for generating bubbles is a gas-liquid mixed shearing method.
- An apparatus useful for generating ultrafine bubbles by the gas-liquid mixed shearing method is, for example, an apparatus disclosed in Japanese Patent No. 4118939.
- this device most of the gas-liquid mixed fluid introduced into the fluid swirl chamber is temporarily directed in the direction opposite to the direction in which the discharge port is located, unlike the conventional device described above. Proceed as a swirl flow. Then, the swirl flow is reversed by the first end wall member and proceeds from the first end wall member toward the second end wall member. At this time, the swirl rotation radius is changed to the first end wall member. Since the flow velocity is smaller than when traveling, the flow velocity becomes high. Therefore, the shearing force to the gas contained in the liquid is increased, and the miniaturization is promoted.
- composition of the present invention in which the sterilizing component is dissolved in water can be produced by treating the aqueous solution of the sterilizing component with an ultrafine bubble generator to generate ultrafine bubbles in the aqueous solution.
- the composition of the present invention can also be produced by dissolving a sterilizing component in water containing ultrafine bubbles.
- the water containing the ultrafine bubbles can have the mode particle size and density as described above.
- the sterilizing component when the sterilizing component is hydrophobic, the sterilizing component can be dispersed in water containing ultrafine bubbles.
- ultrafine bubbles may be generated in the dispersion liquid in which the sterilizing component is dispersed in water, or the sterilizing component may be added and dispersed in water containing the ultrafine bubbles.
- the expression of including a bactericidal component is used, including cases where the bactericidal component is dissolved in water and dispersed.
- the sterilizing components used in the present invention are chlorine sterilizing components, iodine sterilizing components, peroxide sterilizing components, aldehyde sterilizing components, phenolic sterilizing components, biguanide sterilizing components, mercury sterilizing components, alcohol sterilizing components. It can be a component, a quaternary ammonium salt-based bactericidal component, an amphoteric surfactant-based bactericidal component, or a naturally derived antibacterial component.
- chlorinated sterilizing components examples include sodium hypochlorite, chlorine, chlorinated isocyanuric acid and the like.
- iodine-based bactericidal components examples include iodine, povidone iodine, nonoxynoluol, phenoxyiod and the like.
- peroxide-based sterilizing components include hydrogen peroxide, potassium permanganate, ozone, and strongly acidic water.
- aldehyde-based sterilizing components examples include glutaraldehyde, phthalal, formaldehyde and the like.
- phenolic bactericidal ingredients include isopropylmethylphenol, thymol, eugenol, triclosan, cresol, phenol, chlorocresol, parachlorometacresol, parachlorometaxylenol, orthophenylphenol, paraoxybenzoic acid alkyl ester, resorcin, hexachlorophene , Salicylic acid or a salt thereof.
- biguanide-based bactericidal components examples include chlorhexidine, chlorhexidine gluconate, chlorhexidine hydrochloride and the like.
- mercury-based sterilizing components include mercurochrome, mercuric chloride, thimerosal, and the like.
- Examples of the alcohol-based sterilizing component include ethanol and isopropanol.
- quaternary ammonium salt-based bactericidal components include cetylpyridinium chloride, benzethonium chloride, benzalkonium chloride, and decalinium chloride.
- amphoteric surfactant-based bactericidal components include N-alkyldiaminoethylglycine such as N-lauryldiaminoethylglycine and N-myristyldiethylglycine, N-alkyl-N-carboxymethylammonium betaine, 2-alkyl-1hydroxy
- N-alkyldiaminoethylglycine such as N-lauryldiaminoethylglycine and N-myristyldiethylglycine
- N-alkyl-N-carboxymethylammonium betaine N-alkyl-1hydroxy
- 2-alkyl-1hydroxy examples include ethyl imidazoline betaine sodium.
- Antibacterial ingredients derived from natural products include hinokitiol, anethole, anise oil, borneol, camphor, carvone, cassia oil, akaza oil, cineole, citral, citronellal, eugenol, pinene, geraniol, lemon oil, riolol, menthol, orange oil, Plant-based drugs such as safrole and thymol, animal-based drugs such as calcined shell powder obtained by baking chitin, chitosan, scallop and oyster shells made from shellfish shells, and microbial systems such as polylysine Enzymatic drugs such as drugs and lysozyme are listed.
- antibacterial peptides produced by organisms to protect themselves against external microorganisms can also be used, for example, histatin (Defatin), lactoferrin, lactoferrin which is a degradation product of lactoferrin. Examples include thin (Lactofercin), magainin, cecropin, and melittin. Since these are originally produced by living organisms themselves, they have very little side effects or inhibitions on living organisms.
- the sterilization effect of the antibacterial peptide on the skin surface can be enhanced only by washing the body with water containing ultrafine bubbles, and a sufficient sterilization effect can be expected without using a bactericidal agent.
- antibacterial plant extracts can be used as natural antibacterial ingredients.
- specific examples include grapefruit seed extract, red crustaceae, etc., iridaceae, etc., hypericaceae, hypericum perforatum, etc., orchidaceae, gypsophila ginseng, etc., asteraceae, echinacea, chamomile , Burdock, Solidago, Prunus quercus, etc., Ranunculaceae, etc., Honeysuckle, Honeysuckle, etc., Camphoraceae, etc., Mulberry hops, etc.
- Preferred bactericidal components used in the present invention include iodine-based bactericidal components such as povidone iodine, biguanide-based bactericidal components such as chlorhexidine gluconate, quaternary ammonium salt-based bactericidal components such as benzalkonium chloride, and plants such as grapefruit seed extract. An extract is mentioned.
- the amount of the sterilizing component used varies depending on the type and application of the sterilizing component.
- the preferred amount can be appropriately determined by experiment, but generally it can be used in the range of 10 to 0.00001% by weight of the fungicide composition.
- the sterilizing agent composition of the present invention can be blended with any appropriate component depending on the dosage form within a range that does not interfere with the effects of the present invention.
- Thickeners, stabilizers, pH adjusters, preservatives, sweeteners, fragrances, surfactants, active ingredients, colorants, chelating agents, ultraviolet absorbers, bleaching agents, antifoaming agents, enzymes and the like can be contained.
- it can be expected to further improve the bactericidal effect by blending an auxiliary agent that enhances the bactericidal effect.
- povidone iodine is used as a sterilizing component, a component that improves the stability at a low concentration can be blended (Japanese Patent Laid-Open No. 1993-43891).
- sugar alcohols such as butylene glycol, ethylene glycol, xylit, maltite, and lactit, and polyhydric alcohols can be used.
- thickeners carboxymethylcellulose sodium, hydroxyethylcellulose, hydroxypropylcellulose, hydroxypropylmethylcellulose, hydroxymethylethylcellulose, methylcellulose and other cellulosic binders, xanthan gum, carrageenan, guar gum, sodium alginate, cationized cellulose, montmorillonite, gelatin And sodium polyacrylate.
- pH adjusters examples include phthalic acid, phosphoric acid, citric acid, succinic acid, acetic acid, fumaric acid, malic acid and carbonic acid and their potassium, sodium and ammonium salts, ribonucleic acid and its salts, sodium hydroxide, and the like. Can be mentioned.
- preservative examples include benzoates such as sodium benzoate, alkyldiaminoethylglycine hydrochloride, potassium sorbate and the like.
- Sweetening agents include saccharin sodium, aspartame, stevioside, stevia extract, paramethoxycinnamic aldehyde, neohesperidyl dihydrochalcone, perillartin and the like.
- fragrances include eucalyptus oil, winter green oil, cassia oil, clove oil, thyme oil, sage oil, basil oil, cardamom oil, coriander oil, spearmint oil, orange oil, lemon oil, mandarin oil, lime oil, grapefruit Oil, coconut oil, sweetie oil, lavender oil, rosemary oil, laurel oil, camomile oil, caraway oil, marjoram oil, celery oil, bay oil, origanum oil, pine needle oil, neroli oil, lemongrass oil, rose oil , Jasmine oil, Patchouli oil, Iris concrete, Rose absolute, Orange flower absolute, Vanilla absolute, Mango absolute, Patchouli absolute, Ginger oleoresin, Pepper oleoresin, Capsicum oleoresin, Pepper Natural fragrances such as extracts, and fragrances processed by these natural fragrances (front reservoir cut, rear reservoir cut, fractional distillation, liquid-liquid extraction, essence, powder fragrance, etc.), limonene, and pinene , Butano
- surfactant examples include nonionic surfactants, anionic surfactants, cationic surfactants, and amphoteric surfactants.
- anionic surfactant examples include sodium lauryl sulfate and sodium myristyl sulfate.
- Acyl sarcosine salts such as sodium alkyl sulfate, sodium lauroyl sarcosine, sodium myristoyl sarcosine, sodium dodecylbenzenesulfonate, sodium monoglyceride monohydrogen sulfate, sodium lauryl sulfoacetate, N-acyl glutamate such as sodium N-palmitoyl glutamate N-methyl-N-acyl taurine sodium, sodium N-methyl-N-acylalanine, sodium ⁇ -olefin sulfonate, and the like.
- the amphoteric surfactant lauryl dimethylaminoacetic acid betaine, N-coconut oil fatty acid acyl-N-carboxymethyl-N-hydroxyermidinium betaine, and the like can be blended.
- Examples of the colorant include blue No. 1, green No. 3, yellow No. 4, red No. 105, and the like.
- auxiliary agent that enhances the bactericidal effect include anionic surfactants and nonionic surfactants that enhance penetration into biofilms, amphoteric surfactants, cationic surfactants, and sugar alcohols.
- examples of the surfactant include sodium lauryl sulfate, and examples of the sugar alcohol include erythritol, xylitol, and sorbitol.
- cationic surfactants include alkyltrimethylammonium salts such as stearyltrimethylammonium chloride and lauryltrimethylammonium chloride, alkylpyridinium salts such as cetylpyridinium chloride, dialkyldimethylammonium salts such as distearyldimethylammonium chloride, and poly (N, N '-Dimethyl-3,5-methylenepiperidinium), alkyl quaternary ammonium salt, alkyldimethylbenzylammonium salt, alkylisoquinolinium salt; dialkyl morpholinium salt, POE-alkylamine, alkylamine salt, polyamine fatty acid Derivatives; POE-amine fatty acid derivatives; polyamine fatty acid derivatives amyl alcohol fatty acid derivatives, benzalkonium chloride, and benzethonium chloride.
- alkyltrimethylammonium salts such as stearyltrimethylammonium chloride and lau
- nonionic surfactants include polyoxyethylene alkyl ether, polyoxyethylene polyoxypropylene alkyl ether, polyoxyethylene alkylphenyl ether, fatty acid polyglycerol ester, fatty acid sucrose ester, fatty acid alkanolamide, alkylamine oxide, Examples thereof include alkylamidoamine oxide.
- chelating agents include sodium tripolyphosphate, sodium metasilicate, sodium carbonate, sodium hydroxide, potassium hydroxide and other alkaline builders, ethylenediaminetetraacetate (EDTA), N-hydroxyethyl-ethylenediaminetriacetate (HEDTA), and triethanolamine. Can be mentioned.
- UV absorbers include benzophenone (2-hydroxybenzophenone, 2,4-dihydroxybenzophenone, etc.), salicylate ( Phenyl salicylate, 2,4-di-t-butylphenyl-3,5-di-t-butyl-4-hydroxybenzoate), benzotriazole-based [(2′-hydroxyphenyl) benzotriazole, (2 ′ -Hydroxy-5′-methylphenyl) benzotriazole, etc.], acrylic [ethyl-2-cyano-3,3-diphenyl acrylate, methyl-2-carbomethoxy-3- (paramethoxybenzyl) acrylate, etc.], etc. It is done.
- antifoaming agent examples include silicone-based (dimethylpolysiloxane, etc.), mineral oil (spindle oil, kerosene, etc.), metal soap having 12 to 22 carbon atoms (calcium stearate, etc.) and the like.
- the enzyme examples include protease, lipase, amylase, cellulase, and oxidase.
- Each said component is only an illustration to the last, and arbitrary well-known chemical
- medical agents can be used unless the effect of this invention is prevented.
- the compounding quantity of each component is also arbitrary and can be used in the range which does not inhibit the effect of this invention.
- Ultrafine bubbles were generated using "BUVITAS” manufactured by Kyowa Kikai Co., Ltd., which is an ultrafine bubble generator using a gas-liquid mixed shear method, using purified water from the Japanese Pharmacopoeia. .
- the particle size of the generated ultrafine bubbles was measured using a nanoparticle analysis system Nanosite Series (manufactured by NanoSight).
- the test water was diluted with purified water and measured, and the value multiplied by the dilution factor was shown as the measurement result. The measurement results are shown in FIG.
- the horizontal axis indicates the particle size in nm
- the vertical axis indicates the number of nanobubble particles per mL (10 8 particles / mL).
- FIG. 2 the measurement result of the fine bubble about Japanese Pharmacopoeia purified water is shown.
- the mode particle diameter of the water containing the generated ultrafine bubbles was 77 nm
- the particle density at the mode particle diameter was 7.44 ⁇ 10 8 particles / mL
- the total particle density was 4.11 ⁇ 10 10 particles / mL.
- the particle density was very low and no normal distribution was seen, so the measurement result was judged to be noise.
- water containing ultrafine bubbles was prepared in the same manner as described above, and as a comparative example of the blank, water using Japanese Pharmacopoeia purified water was used instead of water containing ultrafine bubbles. .
- Example 1 The bactericidal effect test for Pseudomonas aeruginosa in the organic matter was carried out according to “AOAC Official Method 964.02 Testing Disfectant Sagainst; Pseudomonas aeruginosa”. 1) Preparation of test solution Povidone iodine was prepared as described above, and ultrafine bubbles were diluted with water containing oxygen or purified water, and adjusted so that povidone iodine was 100 mg / L.
- test bacterial solution Cryopreserved strain (Pseudomonas areruginosa NBRC13275) was cultured in Tryptic Soy Agar (Difco, hereinafter referred to as “TSA medium”) at 36 ⁇ 1 ° C. for 18 to 24 hours. This culture was transplanted to Tryptic Soy Broth (Difco, hereinafter referred to as “TSB medium”) and cultured at 36 ⁇ 1 ° C. for 18 to 24 hours. The cultured bacterial solution was prepared to about 10 6 CFU / mL with TSB medium and used as a test bacterial solution.
- test bacteria adherent carrier Place a sterilized biological test cup (stainless steel penicillin cup 441-01, Mutual Riken Glass Co., Ltd., hereinafter referred to as “carrier”) in a 100 mL beaker, and test the amount by which the carrier is completely immersed Bacterial liquid (about 30 to 40 mL) was poured and allowed to stand. After 10 to 15 minutes, the carrier was taken out in a petri dish with sterilized filter paper, and allowed to stand at 36 ⁇ 1 ° C. for 40 ⁇ 2 minutes, so that the organic matter derived from the bacteria and the medium was attached to the carrier by drying. 4) Sterilization test 10 ml of each test solution previously maintained at 25 ⁇ 2 ° C.
- carrier sterilized biological test cup
- Example 2 The bactericidal effect test for Staphylococcus aureus in organic soils is based on the sterilization activity test for residential synthetic detergents and soaps (method determined by the Detergent and Soap Fair Trade Council), and Staphylococcus aureus subsp. Aureus NBRC12732 ) was examined for bactericidal efficacy. 1) Preparation of test solution Water containing nitrogen in the bubbles as ultrafine bubbles was prepared as described above. Grapefruit seed extract was used as a natural antibacterial agent, diluted with water containing ultrafine bubbles or purified water, and adjusted to 0.5%.
- test bacterial solution In a sterilized Erlenmeyer flask, put about 5 mL of 1/2 nutrient medium and sterilized glass beads that have been adjusted to the test temperature (25 ⁇ 1 ° C), and add the appropriate amount of sterilized glass beads. One platinum loop was added to the cells. Stir for 3 minutes with a test tube stirrer. Next, about 1 mL is transferred to a sterilized test tube, an appropriate amount of 1/2 nutrient medium is added, and the mixture is stirred with a test tube stirrer, so that the viable cell count is 2.5 ⁇ 10 8 to 12.5 ⁇ 10 8 cfu / ML.
- test bacteria adherent carrier and sterilization test After allowing to stand at the test temperature for 1 hour, 1.0 mL of a model soil substance (bovine serum albumin aqueous solution, 30 g / L) was added, mixed, and allowed to stand for 2 minutes, and used as the test bacterial solution. 3) Preparation of test bacteria adherent carrier and sterilization test Weigh 0.01 mL of the test bacteria solution again stirred onto a stainless steel disc prepared as described in the disinfecting activity test of the synthetic detergent for soap and soap. And uniformly applied to the surface of the test piece. The petri dish was covered and allowed to stand at 25 ⁇ 1 ° C. until the test bacterial solution was apparently dry. Thereafter, 0.1 mL of the test solution was weighed and uniformly applied to the surface.
- a model soil substance bovine serum albumin aqueous solution, 30 g / L
- the petri dish was covered and left at 25 ⁇ 1 ° C. for 1 minute. Thereafter, inactivation was performed as instructed, and the number of viable bacteria was measured. 4) Results The results are shown in Table 2. The described results were repeated 5 times (n5), and n3 without the minimum and maximum values was displayed as the average value (logarithmic value) of the number of bacteria in the test results.
- the natural antibacterial agent adjusted with water was 4.4, whereas the natural antibacterial agent adjusted with water containing ultrafine bubbles was 2.5, and a clear difference was observed in the bactericidal efficacy between the two.
- Example 3 Bactericidal effect on cariogenic bacteria-forming biofilm 1) Preparation of test solution Povidone iodine was diluted with water containing oxygen or purified water by the ultrafine bubbles prepared as described above, and povidone iodine was 1.0 mg / mL and 10 mg. It adjusted so that it might become / mL. 2) Preparation of test bacterial solution Streptococcus mutans (ATCC 25175) was added to Tryptic soy Broth 0.5% Pre-cultured in a yeast extract-containing medium (hereinafter “TSBY medium”) at 37 ° C. until the OD660 reached 0.6 to 0.8 (10 8 cells / mL) to prepare a test bacterial solution.
- TBSY medium yeast extract-containing medium
- a biofilm was formed by inoculating a test tube with 50 ⁇ L of a test bacterial solution in 4950 ⁇ L of TSBY medium (+ 1% sucrose) and culturing at 37 ° C for 18 hours.
- Sterilization test The culture solution was removed from the test tube on which the biofilm was formed with an aspirator, and the biofilm was washed by adding 5 mL of PBS. After removing PBS, 5 mL of the test solution was treated and reacted while shaking with a shaker (37 ° C., 20 minutes). After 20 minutes, 5 mL of 0.4% w / v Na thiosulfate solution was added to the test tube to inactivate the disinfectant.
- the biofilm formed on the wall surface of the test tube was peeled off using an ultrasonic oscillator, and the biofilm was dispersed by vortex to obtain a microbe count sample solution, and the remaining microbe count in the biofilm was measured.
- the sample solution for measuring the number of bacteria is diluted with a buffer solution, cultured on MS agar medium (37 ° C, 36 to 48 hours), then the number of colonies is counted, and the remaining number in the biofilm The number of bacteria was measured. 6) Results The results are shown in Table 3. The results described were repeated 5 times (n5).
- povidone iodine prepared with purified water had 616.6 cfu and 9.2 cfu in the biofilm, whereas povidone iodine prepared with water containing ultrafine bubbles was 149. 2cfu and 0cfu were found, and a difference was observed in the bactericidal efficacy between the two.
- Example 4 The experiment was performed in the same manner as in Example 1. However, povidone iodine was diluted with water containing oxygen or purified water so that povidone iodine was adjusted to 100 mg / L. In addition, the mode particle density of water containing ultrafine bubbles was adjusted to be on the order of 10 4 , 10 5 , 10 6 , and 10 8 per mL, respectively.
- Example 5 The experiment was performed in the same manner as in Example 1. However, povidone iodine was adjusted so that the ultrafine bubbles produced as described above were diluted with air, water containing C 3 F 8 , or purified water, and povidone iodine was 100 mg / L. The results are shown in Table 5.
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Abstract
Description
しかしこれら殺菌剤の多くは、タンパク質などの有機物による汚れや、多糖やタンパク質などに覆われたバイオフィルムに対しては著しい殺菌力の低下を示していた。そのため実験系の殺菌評価と現場使用系の殺菌評価とのずれを生み、殺菌の増殖を許し、感染の原因となっていた。特にバイオフィルムは、生活環境や産業上で深刻な問題を引き起こす場合があり、注目されている。例えば、住環境ではトイレや台所、浴室等のぬめりやつまり、悪臭の原因となり不快感を与える。温泉施設等の循環式浴槽内で形成されたバイオフィルム中の細菌により感染症が発生する場合もある。一方産業上の問題としては、下水道管や船底にバイオフィルムが形成されることにより腐食を引き起こす事があり、また工場の製造ライン上のバイオフィルムが微生物汚染の原因となることもある。医療関連では、透析等のチューブや内視鏡、コンタクトレンズ等の医療器具に形成されたバイオフィルムが感染源となったり、また、皮膚等の人体組織でのバイオフィルム形成により疾病を引き起こしたりする。ヒト口腔内においては歯に形成するバイオフィルム、いわゆるデンタルプラーク(歯垢)がう蝕や歯周病の原因となることがよく知られている。食品関連では、野菜等の生鮮食料品や加工食品原料及び調理器具に形成されたバイオフィルムが腐敗や食中毒の原因となる。これらに対処するために、現状では実験系で得られた殺菌濃度より遥かに高い濃度を実使用濃度に規定している。
前記超微細気泡の内部は空気、酸素、水素、窒素、炭酸ガス、アルゴン、ネオン、キセノン、フッ素化気体、オゾンおよび不活性化ガスから選択される1種または2種以上の気体であることができる。
また本発明で使用される殺菌成分は、ヨウ素系殺菌成分、過酸化物系殺菌成分、アルデヒド系殺菌成分、フェノール系殺菌成分、ビグアナイド系殺菌成分、水銀系殺菌成分、アルコール系殺菌成分、四級アンモニウム塩系殺菌成分、両性界面活性剤系殺菌成分、天然由来殺菌成分であることができる。
さらに本発明は、最頻粒子径が500nm以下である超微細気泡を含む水と殺菌成分を混合することを含む、殺菌剤組成物の製造方法を提供する。また本発明は、殺菌成分を含む水中に、最頻粒子径が500nm以下である超微細気泡を発生させることを含む、殺菌剤組成物の製造方法を提供する。好ましい態様では、超微細気泡の最頻粒子密度は1万個以上であり、10万個以上であることがさらに好ましい。また、異なる好ましい態様においては、総微粒子密度および粒子径1000nm以下の超微細気泡密度は100万個以上である。
さらに本発明は、本発明にかかる殺菌剤組成物を使用する殺菌方法を提供し、特には、本発明にかかる殺菌剤組成物を、バイオフィムと接触させることを含む殺菌方法を提供する。
なお本明細書においてバイオフィルムとは、微生物が形成する高次構造体をいい、たとえば多糖類などの細胞外高分子化合物(EPS)により結合されて生成されるフィルムをいう。バイオフィルムの例としては先に述べたような、住居のトイレや台所、浴室、温泉施設等の循環式浴槽、下水道管等の各種配管、船底、工場の製造ライン、透析等のチューブ、内視鏡、コンタクトレンズ等の医療器具、人体の皮膚、口腔内、野菜等の生鮮食料品や加工食品原料及び調理器具に形成されたバイオフィルムがあげられる。
また水溶性溶媒、たとえばアルコール、グリコール、グリセリン、エーテル、ケトン、エステルなどを加えることもできる。
天然物由来の抗菌成分としては、ヒノキチオール、アネトール、アニスオイル、ボルネオール、樟脳、カルボン、カッシアオイル、アカザオイル、シネオール、シトラール、シトロネラール、オイゲノール、ピネン、ゲラニオール、レモンオイル、リオロール、メントール、オレンジオイル、サフロール、チモール等の植物系薬剤や、甲殻類の殻を原料としたキチン、キトサン、ホタテやカキの貝殻を焼成処理することによって得られる焼成貝殻粉末などの動物系薬剤や、ポリリジンなどの微生物系薬剤、リゾチームなどの酵素系薬剤が挙げられる。また、生物が外界の微生物に対して自らを防御するために産生する抗菌性ペプチドも使用でき、例えば、ヒスタチン(Histatin)、ディフェンシン(Defensin)、ラクトフェリン(Lactoferrin)、ラクトフェリンの分解産物であるラクトフェリシン(Lactoferrcin)、マガイニン(Magainin)、セクロピン(Cecropin)、メリチチン(Melititin)などがある。これらは、本来生物自らが産生しているものであるため、生体に対しての副作用あるいは阻害作用は極めて小さい。また、超微細気泡を含む水で体を洗い流すだけで、皮膚表面の抗菌性ペプチドの殺菌効果を高め、殺菌剤を用いることなく充分な殺菌効果が期待できることが想像される。
殺菌効果を高める助剤としては、例えば、バイオフィルムへの浸透を高めるアニオン性界面活性剤やノニオン性界面活性剤、両性界面活性剤、カチオン性界面活性剤、及び糖アルコールなどが挙げられ、アニオン性界面活性剤の例として、ラウリル硫酸ナトリウム、糖アルコールの例として、エリスリトール、キシリトール、ソルビトールなどが挙げられる。
カチオン界面活性剤としては、塩化ステアリルトリメチルアンモニウム、塩化ラウリルトリメチルアンモニウム等のアルキルトリメチルアンモニウム塩、塩化セチルピリジニウム等のアルキルピリジニウム塩、塩化ジステアリルジメチルアンモニウム等のジアルキルジメチルアンモニウム塩、塩化ポリ(N,N’-ジメチル-3,5-メチレンピペリジニウム)、アルキル四級アンモニウム塩、アルキルジメチルベンジルアンモニウム塩、アルキルイソキノリニウム塩;ジアルキルモリホニウム塩、POE-アルキルアミン、アルキルアミン塩、ポリアミン脂肪酸誘導体;POE-アミン脂肪酸誘導体;ポリアミン脂肪酸誘導体アミルアルコール脂肪酸誘導体、塩化ベンザルコニウム、塩化ベンゼトニウムが挙げられる。
ノニオン性界面活性剤の例としては、ポリオキシエチレンアルキルエーテル、ポリオキシエチレンポリオキシプロピレンアルキルエーテル、ポリオキシエチレンアルキルフェニルエーテル、脂肪酸ポリグリセリンエステル、脂肪酸ショ糖エステル、脂肪酸アルカノールアミド、アルキルアミンオキサイド、アルキルアミドアミンオキサイド等を挙げることができる。
フェニルサリチレート、2,4-ジ-t- ブチルフェニル-3,5-ジ-t-ブチル-4-ヒドロキシベンゾエートなど)、ベンゾトリアゾール系[(2’-ヒドロキシフェニル)ベンゾトリアゾール、(2’-ヒドロキシ-5’-メチルフェニル)ベンゾトリアゾールなど]、アクリル系[エチル-2-シアノ-3,3-ジフェニルアクリレート、メチル-2-カルボメトキシ-3-(パラメトキシベンジル)アクリレートなど]などが挙げられる。
上記の各成分はあくまでも例示に過ぎず、本発明の効果を妨げない限り、任意の公知の薬剤を使用することができる。また各成分の配合量も任意であり、本発明の効果を妨げない範囲で使用することができる。
気液混合せん断方式による超微細気泡発生装置である株式会社協和機設製の「BUVITAS」により、日本薬局方精製水を使用して、超微細気泡を発生させた。生成した超微細気泡の粒径をナノ粒子解析システム ナノサイトシリーズ(NanoSight社製)により測定した。なお、装置の検出上限を超えた場合は、検水を精製水で希釈し測定を行い、希釈倍率を乗じた値を測定結果として示した。測定結果を図1に示す。図の横軸はnm単位での粒子径を、縦軸は1mL当たりのナノバブル粒子数(108個/mL)を示す。また図2に、日本薬局方精製水についての微細気泡の測定結果を示す。
生成した超微細気泡を含む水の最頻粒子径は77nm、最頻粒子径における粒子密度は7.44×108個/mL、総粒子密度は4.11×1010個/mLであった。
日本薬局方精製水については、粒子密度が非常に少なく、正規分布が見られないことから測定結果はノイズであると判断された。
以下の実施例においては、超微細気泡を含む水は上記と同様に調整され、ブランクの比較例としては、超微細気泡を含む水の代わりに、日本薬局方精製水を使用したものを用いた。
有機物中の緑膿菌に対する殺菌効果
試験は、「AOAC Official Method 964.02 Testing Disinfectantsagainst;Pseudomonas aeruginosa」に準拠し実施した。
1) 試験液の調整
ポビドンヨードを上記のようにして作成された、超微細気泡が酸素を含む水または精製水で希釈し、ポビドンヨードが100mg/Lになるように調整した。
2) 試験菌液の調製
凍結保存した菌株(Pseudomonas areruginosa NBRC13275)をTryptic Soy Agar (Difco,以下「TSA培地」)で36±1℃、18~24時間培養した。この培養菌をTryptic Soy Broth(Difco,以下「TSB培地」)に移植して、36±1℃、18~24時間培養した。培養後の菌液をTSB培地で約106CFU/mLに調製し、これを試験菌液とした。
3) 試験菌付着キャリアの調製
100mLビーカーに滅菌済みの生物検査用カップ(ステンレス製ペニシリンカップ441-01,相互理化学硝子製作所,以下「キャリア」と記載)を入れ、キャリアが完全に浸かる量の試験菌液(30~40mL程度)を注ぎ、静置した。10~15分後、滅菌ろ紙を敷いたシャーレにキャリアを取出し、36±1℃、40±2分間静置して、菌および培地由来の有機物をキャリアへ乾燥付着させた。
4) 殺菌試験
あらかじめ25±2℃に保持した各試験液を50mL容量の遠心管に10mLずつ分取し、ここにカギ型白金線を用いて、試験菌を付着させたキャリアを1個ずつ入れて、25±2℃で20分間作用させた。作用後、不活性化剤SCDLP培地(栄研化学)10mLの入った遠心管にキャリアを1個ずつ移し、キャリアに付着している試験液の殺菌成分を不活性化した。これを、20±2℃で、5分間超音波洗浄機で処理して、キャリアから試験菌を洗い出し、さらにボルテックスミキサーで1分間撹拌したものを、菌数測定用試料液として残存菌数を測定した。また、試験液の代わりに滅菌生理食塩液を用いて同様に操作したものを対照とした。
5) 残存菌数の測定
菌数測定用試料液を原液として、滅菌生理食塩液で10倍段階希釈列を作製し、試料液または希釈液の各1mLを無菌的にシャーレに移し、TSA培地20mLと混合後、固化させて36±1℃で48時間培養した。培養後、培地上に発育した集落を数えて、キャリアあたりの残存試験菌数を求めた(定量下限値10CFU/キャリア)。
6) 結果
結果を表1に示した。記載した結果は、繰返し5回(n5)実施し、最小値と最大値を省いたn3を、試験結果の菌数の平均値(対数値)で表示した。
対照の残存菌数は4.9であった。また、精製水で調整したポビドンヨードは3.9であったのに対し、超微細気泡が酸素を含む水で調整したポビドンヨードは1.4となり両者の殺菌効力に明らかな差が認められた。
有機物汚れ中の黄色ブドウ球菌に対する殺菌効果
試験は、住宅用合成洗剤及び石けんの除菌活性試験(洗剤・石けん公正取引協議会が定める方法)に準拠し、黄色ブドウ球菌(Staphylococcus aureus subsp. aureus NBRC12732)に対する殺菌効力を検討した。
1) 試験液の調整
超微細気泡として窒素を気泡内に含有する水を上記のようにして作成した。
天然抗菌剤としてグレープフルーツ種子エキスを使用し、超微細気泡を含む水または精製水で希釈し、0.5%になるように調整した。
2) 試験菌液の調製
殺菌済みの三角フラスコ内に、試験温度(25±1℃)になじませた1/2ニュートリエント培地約5mLと殺菌済みガラスビーズ適量を入れ、前培養した試験菌の菌体を1白金耳添加した。試験管撹拌機で3分間撹拌した。次いで殺菌済みの試験管に約1mLを移し、適当量の1/2ニュートリエント培地を加え、試験管撹拌機で撹拌し、生菌数を2.5×108から12.5×108cfu/mLに調製した。試験温度で1時間静置した後、モデル汚れ物質(牛血清アルブミン水溶液、30g/L)1.0mLを加え、混合し、2分間静置したものを試験菌液として使用した。
3) 試験菌付着キャリアの調製と殺菌試験
住宅用合成洗剤及び石けんの除菌活性試験の記載の通りに調製されたステンレス鋼製円板上に、再度撹拌した試験菌液を0.01mL量り取り、試験片表面に均一に塗布した。シャーレの蓋をして試験菌液が外見上乾くまで25±1℃で静置した。その後、試験液0.1mLを量り取り、表面に均一に塗布した。シャーレの蓋をして25±1℃で1分間静置した。その後指示通りに不活性化を行い、生菌数を測定した。
4)結果
結果を表2に示した。記載した結果は、繰返し5回(n5)実施し、最小値と最大値を省いたn3を、試験結果の菌数の平均値(対数値)で表示した。
水で調整した天然抗菌剤は4.4であったのに対し、超微細気泡を含む水で調整した天然抗菌剤は2.5となり両者の殺菌効力に明らかな差が認められた。
う蝕原菌形成バイオフィルムに対する殺菌効果
1) 試験液の調整
上記のようにして作成した超微細気泡が酸素を含む水または精製水でポビドンヨードを希釈し、ポビドンヨードが1.0mg/mL、および10mg/mLになるように調整した。
2) 試験菌液の調製
Streptococcus mutans(ATCC25175)をTryptic soy Broth 0.5%
Yeast extract含有培地(以下「TSBY培地」)で、37℃でOD660が0.6~0.8(108個/mL)になるまで前培養し試験菌液とした。
3) バイオフィルムの形成
試験管に試験菌液50μLをTSBY培地(+1%スクロース)4950μLに植菌し、37℃で18時間培養することでバイオフィルムを形成させた。
4) 殺菌試験
バイオフィルムを形成させた試験管からアスピレーターで培養液を除去し、PBS5mLを添加することでバイオフィルムを洗浄した。PBSを除去後、試験液5mLを処理し、振蕩機で揺らしながら反応させた(37℃、20分)。20分後、試験管に0.4%w/vチオ硫酸Na溶液5mLを加え殺菌剤を不活化した。超音波発振機を用いて試験管壁面に形成されたバイオフィルムを剥離し、ボルテックスにてバイオフィルムを分散させ菌数測定用試料液とし、バイオフィルム中の残存菌数を測定した。
5) バイオフィルム中残存菌数の測定
菌数測定用試料液を緩衝液で希釈し、MS寒天培地で培養(37℃、36~48時間)後、コロニー数を計数し、バイオフィルム中の残存菌数を測定した。
6)結果
結果を表3に示した。記載した結果は、繰返し5回(n5)実施した。
精製水で調整した1.0mg/mLおよび10mg/mLポビドンヨードはバイオフィルム中の菌数が616.6cfuおよび9.2cfuであったのに対し、超微細気泡を含む水で調整したポビドンヨードは149.2cfuおよび0cfuとなり両者の殺菌効力に差が認められた。
実施例1と同じ方法により、実験を行った。
ただし、ポビドンヨードを上記のようにして作成された超微細気泡が酸素を含む水または精製水で希釈し、ポビドンヨードが100mg/Lになるように調整した。また、超微細気泡を含む水の最頻粒子密度を、それぞれ1mLあたり104、105、106、108のオーダーになるように調整した。
実施例1と同じ方法により、実験を行った。
ただし、ポビドンヨードを上記のようにして作成された超微細気泡が大気またはC3F8を含む水、または精製水で希釈し、ポビドンヨードが100mg/Lになるように調整した。
結果を表5に示す。
Claims (9)
- 最頻粒子径が500nm以下である超微細気泡を含む水と殺菌成分を含む殺菌剤組成物。
- 超微細気泡の最頻粒子密度が1万個以上である、請求項1に記載の殺菌剤組成物。
- 粒子径1000nm以下の超微細気泡密度が100万個以上である、請求項1または請求項2に記載の殺菌剤組成物。
- 前記超微細気泡が空気、酸素、水素、窒素、炭酸ガス、アルゴン、ネオン、キセノン、フッ素化気体、オゾンおよび不活性化ガスから選択される1種または2種以上の気体でなる、請求項1から3のいずれか1項記載の殺菌剤組成物。
- 前記の殺菌成分が、塩素系殺菌成分、ヨウ素系殺菌成分、過酸化物系殺菌成分、アルデヒド系殺菌成分、フェノール系殺菌成分、ビグアナイド系殺菌成分、水銀系殺菌成分、アルコール系殺菌成分、四級アンモニウム塩系殺菌成分、両性界面活性剤系殺菌成分、天然由来抗菌剤である、請求項1から4のいずれか1項記載の殺菌剤組成物。
- 最頻粒子径が500nm以下である超微細気泡を含む水と殺菌成分を混合することを含む、殺菌剤組成物の製造方法。
- 殺菌成分を含む水中に、最頻粒子径が500nm以下である超微細気泡を発生させることを含む、殺菌剤組成物の製造方法。
- 請求項1から5のいずれか1項記載の殺菌剤組成物を用いることを特徴とする殺菌方法。
- 請求項1から5のいずれか1項記載の殺菌剤組成物を、バイオフィムと接触させることを含む殺菌方法。
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EP13755549.6A EP2820951A4 (en) | 2012-02-29 | 2013-02-22 | BACTERICIDE COMPOSITION |
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SG11201404577XA SG11201404577XA (en) | 2012-02-29 | 2013-02-22 | Bactericidal agent composition |
AU2013227556A AU2013227556B2 (en) | 2012-02-29 | 2013-02-22 | Bactericidal agent composition |
CN201380010672.4A CN104135856A (zh) | 2012-02-29 | 2013-02-22 | 杀菌剂组合物 |
CA2864079A CA2864079A1 (en) | 2012-02-29 | 2013-02-22 | Bactericidal agent composition |
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CA (1) | CA2864079A1 (ja) |
MY (1) | MY166868A (ja) |
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WO2015071995A1 (ja) * | 2013-11-14 | 2015-05-21 | 古米 保 | 超微細気泡含有微酸性次亜塩素酸水溶液とその製造方法及び使用方法 |
CN105377770A (zh) * | 2013-11-14 | 2016-03-02 | 株式会社石桥 | 含有超微细气泡的微酸性次亚氯酸水溶液和其制作方法及使用方法 |
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WO2023063361A1 (ja) * | 2021-10-15 | 2023-04-20 | 合同会社琉球ワンダー | 除菌液 |
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AU2013227556B2 (en) | 2016-05-19 |
SG11201404577XA (en) | 2014-10-30 |
TWI578909B (zh) | 2017-04-21 |
AU2013227556A1 (en) | 2014-09-11 |
CA2864079A1 (en) | 2013-09-06 |
EP2820951A4 (en) | 2015-11-11 |
MY166868A (en) | 2018-07-24 |
CN104135856A (zh) | 2014-11-05 |
US20150258232A1 (en) | 2015-09-17 |
TW201402008A (zh) | 2014-01-16 |
JP2013180956A (ja) | 2013-09-12 |
US9220799B2 (en) | 2015-12-29 |
EP2820951A1 (en) | 2015-01-07 |
US20150010604A1 (en) | 2015-01-08 |
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