WO2012108557A1 - 코디세핀 성분이 다량 함유된 기능성 쌀 및 그 생산방법 - Google Patents

코디세핀 성분이 다량 함유된 기능성 쌀 및 그 생산방법 Download PDF

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Publication number
WO2012108557A1
WO2012108557A1 PCT/KR2011/000843 KR2011000843W WO2012108557A1 WO 2012108557 A1 WO2012108557 A1 WO 2012108557A1 KR 2011000843 W KR2011000843 W KR 2011000843W WO 2012108557 A1 WO2012108557 A1 WO 2012108557A1
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WIPO (PCT)
Prior art keywords
cordycepin
rice
large amount
functional rice
mixing
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PCT/KR2011/000843
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English (en)
French (fr)
Korean (ko)
Inventor
진광식
Original Assignee
팜스코리아영농조합법인
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Priority to CN2011800151932A priority Critical patent/CN102821619B/zh
Publication of WO2012108557A1 publication Critical patent/WO2012108557A1/ko

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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L19/00—Products from fruits or vegetables; Preparation or treatment thereof
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L7/00—Cereal-derived products; Malt products; Preparation or treatment thereof
    • A23L7/10—Cereal-derived products
    • A23L7/104—Fermentation of farinaceous cereal or cereal material; Addition of enzymes or microorganisms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L7/00—Cereal-derived products; Malt products; Preparation or treatment thereof
    • A23L7/10—Cereal-derived products
    • A23L7/196—Products in which the original granular shape is maintained, e.g. parboiled rice

Definitions

  • the present invention relates to a functional rice containing a large amount of cordycepin, an important functional component of Cordyceps sinensis, and a method of producing the same, and more particularly, using deep-sealed rice, vegetable carbon fiber, and LED lamps as a medium.
  • the present invention relates to a method of producing a plant by incubating Cordyceps sinensis directly in rice to increase the content of cordycepin, a functional ingredient.
  • Cordyceps sinensis refers to Cordycepes sinensis from the larvae of the bat moth, but today, mushrooms from spiders, fungi, etc. as well as insects are collectively called cordyceps, and chrysalis and snowhoppers are the species of lepidoptera. It can be said that they have a common point in that they can be based on pupa.
  • Cordyceps sinensis is difficult to collect the fruiting body in the natural state, the research on the spawn culture and cultivation method using silkworm pupa, and the mass cultivation method using artificial medium has been actively conducted in recent years.
  • Korean Patent Laid-Open Publication No. 98-033558 discloses "Cultivation method of mixing pupa and grains to form cordyceps fruiting body", Korean Patent Publication No. 97- No. 38332, "Method and Cultivation of Seedling of Chrysalis of Cordyceps sinensis Using Silkworm Chrysalis" Registration No.
  • the technology using deep seawater, vegetable carbon fiber and LED lamp to infiltrate bacteria deep into the rice and increase the content of cordycepin, which is an important functional component of Cordyceps sinensis, is used as a medium. None was reported.
  • Cordyceps sinensis is belonging to asymptomatic fungus, nucleus fungus, pulmonary fungus, Cordyceps sinensis, which is identified as an isomer of quinic acid, and contains a substance called “codeispin”. It has excellent effects on improving stamina, lowering blood sugar level and inhibiting cancer cell growth.
  • Cordycepin is a substance called D-mannitol that enters the blood and circulates, lowering head pressure, reducing hydrocephalus, and reducing eye pressure and diuresis.
  • the present invention aims to produce rice containing a large amount of cordycepin components of Cordyceps sinensis by easily cultivating Cordyceps fungi in rice.
  • the present invention relates to a method for producing a functional rice containing a large amount of cordycepin, which is a functional component of Cordyceps sinensis, in order to revive the conventional demand as described above. If the conventional technique is simply applying mycelium to rice, this method is to express the unique color and efficacy of natural mushrooms in rice as much as possible to increase its efficacy.
  • the fruiting body and mycelium of Cordyceps sinensis in a large amount of rice by using deep ocean water, vegetable carbon fiber, and LED lamp in a large amount of rice to increase the content of cordycepin To provide.
  • the present invention relates to a method for producing functional rice in which a mycelium containing cordycepin component, which is an important functional component of Cordyceps sinensis according to the present invention, is multiplied in rice, and has a functional ingredient that Cordyceps has in the food science efficacy of rice. It is a great invention that can be used as a material of medical food that can directly help people's health by forming a complex of.
  • 3 is cordycepin content table according to deep sea water treatment.
  • 5 is cordycepin content table according to the LED (LED) lamp.
  • the functional rice production method containing the components of Cordyceps sinensis according to the present invention includes a mixing step, sterilization and cooling step, container holding and culture inoculation step, dark and clear room culture step, drying, packaging and product step (Fig. One).
  • the foreign material is filtered out, the clean rice (2010, Yeoju) is polished with white rice, and the milled rice and deep sea water are mixed with 100: 20 ⁇ 100 (w / w), and 500g of the mixture is sterilized (1,000 milliliters).
  • the vegetable carbon fiber nonwoven fabric in the Erlenmeyer flask vessel should contain 2 centimeters by 2 centimeters in length.
  • Cordyceps sinensis is a type of mushroom. Unlike ordinary mushrooms that nourish soil or twigs, they grow on animal (insect) nutrients and are parasitic to dead insects in winter, but bloom in summer. It is widely distributed throughout the world, including Korea and China and Japan. There are about 800 species of Cordyceps invaded by insects and there are about 78 species in Korea. Representatives include chrysalis cordyceps, large cicada cordyceps, large caterpillar cordyceps, snow flower cordyceps and bee cordyceps.
  • Cordycepin (Cordycepin) is not only effective as an immune booster, as a natural antibiotic, but also has an excellent effect on strengthening sexual function, fatigue, stamina, lowering blood sugar level and inhibiting cancer cell development.
  • Cordyceps sinensis contains an important substance (nucleic acid substance) known as an isomer of quinic acid (nucleic acid substance), which is involved in the genetic information of the cell and activates the reduced immune function to prevent normal cells from turning into cancer cells. It is known that.
  • Deep seawater is a seawater that is more than 200m deep without solar light.
  • deep ocean water is water deep in the sea below 200 meters deep, and has a different nature from that of the sea surface.
  • Water in the deep sea is not contaminated, but instead rich in nutrients and inorganic salts such as nitrogen, phosphorus and silicon, and the water temperature is maintained at about 2 °C, and its features include low temperature storage, cleanliness, minerality, and rich nutrition. Has characteristics. In recent years, it has been in the spotlight as its utilization and importance are increasing in various fields such as seafood, beverage, cosmetics, and medicine.
  • the natural vegetable carbon fiber using charcoal is carbonized by firing in a vacuum close to the shape of the fiber.
  • the nonwoven fabric of filter shape is 2mm thick and 4.5 times the pore area of ordinary charcoal. have. It has deodorizing effect, such as antibacterial effect, far infrared radiation, anion generation and electromagnetic wave blocking effect.
  • FIG. 3 The change in cordycepin content in rice cultured with Cordyceps mycelium according to deep seawater treatment is shown in FIG. 3, and the change in cordycepin content in rice cultured with Cordyceps mycelium according to the treatment with vegetable carbon fiber is shown in FIG. 4.
  • a glass triangle plastic flask (1,000 ml) containing vegetable carbon fiber mixed with deep sea water of rice (1) and sterilized in a autoclave pot is sterilized at 121 ° C. for 10-30 minutes, and then taken out by natural cooling method. Keep the temperature at 20 ⁇ 25 °C.
  • Cordyceps fungus (Cordicephs mitaris) is grown in a dark room at room temperature of 10 to 15 rpm using a sterile potato medium (PDA medium) in the contents of step (2) at room temperature. Incubate 20% (W / v) aseptically with 500g of the contents in one liquid culture.
  • PDA medium sterile potato medium
  • step (3) the contents of step (3) are incubated for 2 to 8 days in a dark room in a clean culture room where the temperature of 20 to 25 ° C. and humidity of 70% or more are continuously maintained, followed by the temperature of 20 to 25 ° C. in the next step. And bright cultivation of the incubation chamber to more than 1000 lux with 10% cultivation of the cultivation of more than 70% of the humidity. After 10 days of incubation, the upper part receiving the light has a unique red or yellow color of mushroom mycelia. Incubate the lower part upward to stop the culture when the mycelium is completely colored.
  • LED lamps have been studied in the 1990s by the US Aeronautics and Space Administration as a light source for photosynthesis for plant cultivation to feed crews in space stations and spacecraft.
  • LED is divided into Infrared Emitting Diode (IRD) and Visible Light Emitting Diode (VLED).
  • IFD Infrared Emitting Diode
  • VLED Visible Light Emitting Diode
  • the chip is assembled into a lamp (LED) and is used as a light source for various electronic products, signs and signal boards.
  • LED is a semiconductor, which is attracting attention as a light source of advanced lighting because of its great advantages in terms of processing speed, power consumption, and lifespan. Recent studies have shown that LED light is absorbed by the human skin to promote blood circulation, and is absorbed by cells to promote healing time by more than 50% and relieve muscle tension.
  • the cultured functional rice is poured out of the container and shaken appropriately so that the rice particles do not stick together.
  • the final product is dried at 50-70 ° C. for 15-30 hours using a hot air dryer (EPD-360R, Korea). Be sure to
  • the high-speed liquid chromatography (HPLC) measurement standard curve for measuring cordycepin content has a retention time of 26.398 (cordycepin injection content 6.25 ⁇ g / mL),
  • Cordycepine content measured according to the deep seawater treatment of the present invention was shown as FIG. According to the present invention, the method for measuring the cordycepin content of rice cultured with deep seawater-treated Cordyceps mycelia is as described above.
  • the content of cordycepin in functional rice cultured at 100: 20 to 100 (w / w) in the ratio of raw rice and deep sea water was 100, 20 to 100 (w / w), ranging from 3,21 to 20.72 (ug / g).
  • the depth mixing ratio was excellent between 100: 60 ⁇ 80 (w / w), and the best ratio was obtained when set to 60.
  • the present invention is shown in Figure 4 by measuring the cordycepin content according to the vegetable carbon fiber (nonwoven fabric) containing one of the preferred practice. At this time, the method of measuring the content of cordycepin was performed in the same manner as in the embodiment of the deep sea water treatment.
  • the content of cordycepin in the final cultured functional rice is included when it is included or does not include vegetable carbon fiber in the container. 20.30 (ug / g) was included and 10.21 (ug / g) was not included.
  • the present invention is shown in Figure 5 by measuring the content of cordycepin according to the brightness of the LED (LED) lamp of one of the preferred embodiments. At this time, the content of cordycepin was measured in the same manner as in the previous example.
  • the ratio of raw rice and deep sea water was 100: 60 (w / w), and the LED (LED) lamp brightness was treated at 1,000 to 5,000 lux, and the final cultured cordycepin content was 13.33 to 21.66 ( ug / g), LED lamps had excellent brightness between 3000 and 5000 lux, and 3,000 lux were the best.

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Mycology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Mushroom Cultivation (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
PCT/KR2011/000843 2011-02-08 2011-02-09 코디세핀 성분이 다량 함유된 기능성 쌀 및 그 생산방법 WO2012108557A1 (ko)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN2011800151932A CN102821619B (zh) 2011-02-08 2011-02-09 含有大量虫草素成分的功能性米及其生产方法

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Application Number Priority Date Filing Date Title
KR1020110011085A KR101160069B1 (ko) 2011-02-08 2011-02-08 코디세핀 성분이 다량 함유된 기능성 쌀 및 그 생산방법
KR10-2011-0011085 2011-02-08

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111826410A (zh) * 2020-08-10 2020-10-27 辽东学院 一种利用固体培养基获得高产虫草素的方法

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104286838B (zh) * 2014-07-29 2016-08-24 湖北省农业科学院农产品加工与核农技术研究所 一种富含虫草素的虫草重组米及其制备方法
CN104381010B (zh) * 2014-10-08 2017-09-15 生展生物科技股份有限公司 冬虫夏草子实体的培养方法及其组合物和应用
KR102063578B1 (ko) * 2018-08-07 2020-02-11 이상복 가바와 코디세핀 성분이 향상된 동충하초 균사체 발아 곡물 제조방법
KR20230057517A (ko) 2021-10-21 2023-05-02 이용화 유효성분이 함유된 기능성 쌀 및 그 제조방법
KR20230124116A (ko) 2022-02-17 2023-08-25 김홍일 코디세핀이 함유된 기능성 쌀의 제조 방법
KR20230171186A (ko) 2022-06-13 2023-12-20 주식회사 인쎌텍 생명과학 코디세핀 쌀 추출물을 함유하는 화장료 조성물

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20030082036A (ko) * 2002-04-16 2003-10-22 이재성 약용 버섯균을 배양한 기능성 쌀 및 그 제조방법
KR20040071826A (ko) * 2003-02-07 2004-08-16 홍종덕 코디세핀이 함유된 기능성 쌀 및 그 제조방법
KR100462497B1 (ko) * 2002-02-22 2004-12-17 주식회사 덕승바이오 약용버섯류(동충하초, 상황버섯)의 균사체를 배양한기능성 쌀의 생산방법
KR20080104673A (ko) * 2007-05-29 2008-12-03 김규종 심층수 함유 균배양쌀
KR20090076425A (ko) * 2008-01-09 2009-07-13 서희동 해양 심층수를 이용하여 기능미를 제조하는 방법
KR20090091260A (ko) * 2008-02-24 2009-08-27 서희동 해양 심층수로부터 생산된 미네랄 수를 이용하여 기능미를제조하는 방법

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20020085517A (ko) * 2001-05-09 2002-11-16 오종명 곡물을 이용한 식용버섯종균 제조방법
CN101575569A (zh) * 2008-05-05 2009-11-11 邬咲博 培养冬虫夏草的方法
CN101575570A (zh) * 2008-05-05 2009-11-11 邬咲博 作用于北冬虫夏草培养气体的方法
KR20100119529A (ko) * 2010-09-27 2010-11-09 서희동 해양 심층수로부터 생산된 미네랄 수를 이용하여 기능미를 제조하는 방법

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100462497B1 (ko) * 2002-02-22 2004-12-17 주식회사 덕승바이오 약용버섯류(동충하초, 상황버섯)의 균사체를 배양한기능성 쌀의 생산방법
KR20030082036A (ko) * 2002-04-16 2003-10-22 이재성 약용 버섯균을 배양한 기능성 쌀 및 그 제조방법
KR20040071826A (ko) * 2003-02-07 2004-08-16 홍종덕 코디세핀이 함유된 기능성 쌀 및 그 제조방법
KR20080104673A (ko) * 2007-05-29 2008-12-03 김규종 심층수 함유 균배양쌀
KR20090076425A (ko) * 2008-01-09 2009-07-13 서희동 해양 심층수를 이용하여 기능미를 제조하는 방법
KR20090091260A (ko) * 2008-02-24 2009-08-27 서희동 해양 심층수로부터 생산된 미네랄 수를 이용하여 기능미를제조하는 방법

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111826410A (zh) * 2020-08-10 2020-10-27 辽东学院 一种利用固体培养基获得高产虫草素的方法

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