Classifications

• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K8/00—Cosmetics or similar toiletry preparations
  • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
  • A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
  • A61K8/73—Polysaccharides
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
  • A61Q11/00—Preparations for care of the teeth, of the oral cavity or of dentures; Dentifrices, e.g. toothpastes; Mouth rinses
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K8/00—Cosmetics or similar toiletry preparations
  • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
  • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
  • A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
  • A61K8/34—Alcohols
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K8/00—Cosmetics or similar toiletry preparations
  • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
  • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
  • A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
  • A61K8/34—Alcohols
  • A61K8/347—Phenols
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K8/00—Cosmetics or similar toiletry preparations
  • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
  • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
  • A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
  • A61K8/39—Derivatives containing from 2 to 10 oxyalkylene groups
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K8/00—Cosmetics or similar toiletry preparations
  • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
  • A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
  • A61K8/73—Polysaccharides
  • A61K8/733—Alginic acid; Salts thereof
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K8/00—Cosmetics or similar toiletry preparations
  • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
  • A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
  • A61K8/84—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds obtained by reactions otherwise than those involving only carbon-carbon unsaturated bonds
  • A61K8/86—Polyethers

Definitions

• the present invention has a high bactericidal power against periodontopathic biofilms formed by periodontal pathogenic bacteria in the oral cavity and the effect of suppressing oral adhesion of oral bacteria to the tooth surface, and is successfully exhibited after long-term storage. It is related with the dentifrice composition which can suppress the production
• caries is an infection caused by bacteria such as Streptococcus mutans (Streptococcus mutans), and periodontal disease is mainly anaerobic gram-negative bacilli such as Porphyromonas gingivalis.
• oral bacteria such as Fusobacterium nucleatum are involved as a cause of bad breath. Therefore, as an effective means for preventing and improving periodontal disease, keep the number of pathogenic bacteria in periodontopathic biofilm at a low level, and further suppress the adhesion of periodontal bacteria in the oral cavity. It is said that it is useful to suppress the generation of periodontopathic biofilm.
• isopropylmethylphenol acts on periodontopathic biofilms generated in periodontal pockets for reasons such as inadequate oral cleaning, and suppresses tooth surface adhesion of oral bacteria. There was a problem that the effect of suppressing the generation of periodontopathic biofilm was low.
• propylene glycol alginate is mainly used as a binder of a dentifrice composition, and is used for the purpose of improving liquid separation and viscosity reduction of toothpaste (see Patent Documents 5 and 6). It is used for suppressing the adsorption of fat-soluble vitamins in a container containing a semi-solid preparation (see Patent Document 7), or used for masking tastes derived from essential oils (see Patent Document 8).
• a composition that has both stable retention of fluoride colloid and prevention of dental caries can be obtained by using water-soluble hydrofluoric acid salt and water-soluble calcium salt together with substances that promote their colloidalization. It is disclosed (see Patent Document 9).
• triclosan with an intramolecular mannuronic acid / guluronic acid ratio (M / G ratio) of 1.0 or less is used in combination with triclosan to increase oral retention and prevent plaque formation and prevent gingivitis.
• the composition for oral cavity which improved the effect is disclosed (refer patent document 10).
• the effect of using an alginate with an M / G ratio of 1.0 or less in combination with triclosan suppresses the formation of biofilm itself by improving the retention of triclosan in the oral cavity.
• the bactericidal effect against bacteria inside periodontopathic biofilm was not enough.
• the number of pathogenic bacteria in the periodontopathic biofilm which is an effective means for preventing and improving periodontal disease, is kept at a low level, and further, the generation of periodontopathic biofilm is suppressed. It has not yet been achieved to achieve both at a sufficient level.
• the present invention has been made to solve the above-mentioned problems, and the bactericidal power against periodontopathogenic biofilms formed by oral periodontal pathogenic bacteria and the effect of suppressing oral adhesion of oral bacteria on tooth surfaces are satisfied after long-term storage. It is an object of the present invention to provide a dentifrice composition that can be effectively exhibited and can effectively suppress the generation of periodontopathic biofilms.
• the present inventors have used (A) propylene glycol alginate and (B) isopropylmethylphenol in combination, and (C) the alkyl group has 14 to 20 carbon atoms. 18, at least one nonion selected from polyoxyethylene alkyl ethers having an average addition mole number of ethylene oxide of 2 to less than 20 and polyoxyethylene hydrogenated castor oil having an average addition mole number of ethylene oxide of 5 to 30
• polyoxyethylene alkyl ethers having an average addition mole number of ethylene oxide of 2 to less than 20
• polyoxyethylene hydrogenated castor oil having an average addition mole number of ethylene oxide of 5 to 30
• it has excellent anti-adhesion effect on the tooth surface of oral bacteria, especially periodontal disease-causing bacteria. Is effectively demonstrated after long-term storage, and thus dentifrice can effectively suppress the generation of periodontopathic biofilm It found that composition is obtained, and have completed the present invention.
• a dentifrice composition containing a poorly water-soluble disinfectant such as isopropylmethylphenol has a bactericidal effect on oral biofilms, but the effect is still not sufficient and there is room for improvement. Even after brushing, bacteria remain in the oral cavity, and the bacteria may adhere to and proliferate again in the oral cavity, which may adversely affect the periodontal tissue.
• the present inventors examined non-bactericidal plaque control means, and added alginic acid propylene glycol ester to the tooth surface of periodontal disease-causing bacteria, particularly Porphyromonas gingivalis. It has been found that there is a previously unknown effect of suppressing adhesion and it is useful for plaque control.
• the tooth surface of oral bacteria It is excellent in both anti-adhesion effect and bactericidal effect on periodontopathic biofilm, and even if the preparation is stored for a long time, these excellent characteristics are exhibited, and it is excellent in periodontopathic biofilm suppression effect I found it.
• propylene glycol alginate has an effect of suppressing the adhesion of the tooth surface of periodontal disease-causing bacteria and enhances the biofilm bactericidal effect.
• the mechanism of action is not clear, but by using alginate propylene glycol ester in combination with isopropylmethylphenol, these two components act synergistically and become the initial stage of generation of periodontopathic biofilm.
• the preparation is coated on the tooth surface and isopropylmethylphenol is sufficiently retained, so that it remains in the interdental and gingival margins where cleaning is not possible. Therefore, it is presumed that an excellent bactericidal effect is exhibited even for a periodontopathic biofilm having a high drug barrier performance.
• a lipophilic nonionic surfactant it is possible to improve the above-mentioned effects and improve the extrudability of the preparation at low temperatures.
• the present invention provides the following dentifrice composition.
• a dentifrice composition comprising at least one nonionic surfactant selected from polyoxyethylene hydrogenated castor oil having an average addition mole number of ethylene alkyl ether and ethylene oxide of 5 to 30.
• the dentifrice composition according to [I] wherein the blending ratio of the component (A) to the component (B) is 0.4 to 20 as a mass ratio of (A) / (B).
• the dentifrice composition of the present invention is such that the bactericidal effect on periodontopathic biofilms and the effect of suppressing initial adhesion of periodontopathic bacteria to the tooth surface are effectively exhibited after long-term storage, such as periodontal disease It is useful for the prevention or improvement of oral diseases. Furthermore, according to the present invention, it is possible to provide a preparation having the above-mentioned excellent effects and excellent extrudability at a low temperature.
• the dentifrice composition of the present invention is suitably prepared as a dentifrice such as a toothpaste, a liquid dentifrice, and a toothpaste, particularly a toothpaste, and comprises (A) propylene glycol ester alginate, (B) isopropylmethylphenol, (C) A polyoxyethylene alkyl ether having an alkyl group with 14 to 18 carbon atoms and an average addition mole number of ethylene oxide of 2 to less than 20, and a polyoxyethylene hydrogenated castor oil with an average addition mole number of ethylene oxide of 5 to 30 Contains at least one nonionic surfactant selected.
• Propylene glycol alginate is blended as a periodontal disease causative agent inhibitor.
• Propylene glycol alginate is an ester made by introducing a propylene glycol group into the carboxyl group in order to increase the acid resistance and salt resistance of the natural polysaccharide alginic acid that is unique to brown algae represented by kombu and wakame.
• Commercially available products such as Kemifa Duck Lloyd, Kimika Kimiroid, and kelp acid can be used.
• Propylene glycol ester of alginic acid is obtained by esterifying the carboxyl group of alginic acid with propylene oxide.
• various degrees of polymerization that is, various molecular weights, various intramolecular ⁇ -D-mannuronic acid / ⁇ -L-guluronic acid Quantitative ratios (M / G ratio, molar ratio, the same applies hereinafter) and various esterification degrees can be used.
• particularly preferred propylene glycol alginate has an M / G ratio of more than 1.0, particularly preferably more than 1.0 and 2 or less.
• the esterification degree of the carboxyl group is preferably 40% or more, particularly preferably 70% or more, and particularly preferably 70 to 95%.
• the viscosity at 20 ° C. (hereinafter the same) of a 1% (mass%, the same applies hereinafter) aqueous solution measured by a B-type viscometer described later is 10 to 1,300 mPa ⁇ s, particularly 10 to 200 mPa ⁇ s. It is preferable that it is the range of these. Those having a viscosity of less than 10 mPa ⁇ s are not commercially available, and if it exceeds 1,300 mPa ⁇ s, there is a possibility that sufficient antifouling effect of periodontal disease-causing bacteria and enhancement of biofilm bactericidal effect may not be obtained. .
• alginate propylene glycol ester Kimika's Kimiloid BF, LLV, NLSK, LV, MV, HV, kelp acid 501, 503, 542, Food Chemifa's Duck Lloyd LF, PF, etc. are commercially available. Goods can be used.
• the propylene glycol alginate can be used alone or in combination of two or more depending on the type of product. Specifically, the following commercially available products can be used.
• Alginic acid propylene glycol ester manufactured by Kimika Co., Ltd., trade name: Kimiloid BF, M / G ratio: 1.3, degree of esterification: 80%, viscosity of 1% aqueous solution (20 ° C.): 20 mPa ⁇ s (rotor no. 1, 60 rpm)
• Propylene glycol ester alginate manufactured by Kimika Co., Ltd., trade name: Kimiloid LV, M / G ratio: 1.3, degree of esterification: 80%, viscosity of 1% aqueous solution (20 ° C.): 90 mPa ⁇ s (rotor no.
• the viscosity is a value measured by a BL type viscometer, and specifically, a measured value by the following method.
• Viscosity measurement method Digi Lloyd, Food Chemifa Corporation 4 g of propylene glycol alginate is collected and placed in a 600 mL beaker, and 396 g of purified water is added little by little while stirring with a stir bar. First, dissolve well with a small amount of water. Thereafter, the mixture is allowed to swell for 1 hour, and after 1 hour, the mixture is stirred for 1 minute at 12,000 rpm with a high-speed stirrer (homomixer). This solution is put into a 300 mL tall beaker and allowed to stand in a 20 ° C. water bath.
• Viscosity measurement method Korean, Kimlicum, Kimika Co., Ltd.
• a 300 mL tall beaker 297 g of purified water is taken and 3.0 g of alginic acid propylene glycol ester is added and completely dissolved while stirring with a stirrer or three-one motor.
• the viscosity after 1 minute is measured correctly using BL type
• Viscometer Tokyo Keiki BL type viscometer measurement conditions ⁇ When 1% aqueous solution viscosity is 10 to 80 mPa ⁇ s: Rotor No. 1.
• the blending amount of propylene glycol alginate is preferably 0.05 to 1%, particularly 0.1 to 1% of the whole composition. If it is less than 0.05%, the sufficient effect of suppressing the adhesion of periodontal disease-causing bacteria may not be exhibited, and if it exceeds 1%, there may be a problem in extrudability at low temperatures or the surface of the composition may become rough.
• Isopropylmethylphenol is 1-hydroxy-4-isopropyl-3-methylphenol, and a product sold by Osaka Kasei Co., Ltd. under the trade name Biosol can be used.
• the blending amount is preferably 0.01 to 0.2%, particularly 0.03 to 0.2% of the whole composition. If it is less than 0.01%, a sufficient bactericidal effect may not be exhibited. If it exceeds 0.2%, the taste may be deteriorated, or the surface of the composition after storage may become rough.
• the mass ratio ((A) / (B)) of the component (A) to the component (B) is preferably 0.4 or more and 20 or less, particularly 1 to 10.
• Component (C) is a nonionic surfactant, a polyoxygen having an alkyl group with 14 to 18 carbon atoms and an average added mole number of ethylene oxide (hereinafter abbreviated as EO) of 2 or more and less than 20.
• Ethylene (hereinafter abbreviated as POE) alkyl ether and EO are one or more selected from POE hydrogenated castor oil having an average number of added moles of 5 to 30, and the POE alkyl ether and POE hydrogenated castor oil You may use together.
• the carbon number of the alkyl group of the POE alkyl ether is 14 to 18, preferably 16 to 18. When the number of carbon atoms of the alkyl group is smaller than 14 or larger than 18, sufficient bactericidal effect is not exhibited in the composition after storage.
• Specific examples include POE cetyl ether, POE myristyl ether, POE stearyl ether, and POE stearyl ether is particularly preferable.
• the average number of moles of EO added to the POE alkyl ether is in the range of 2 to 20 moles, preferably 5 to 10 moles. If the average added mole number is less than 2 moles or 20 moles or more, the composition after storage may not exhibit a sufficient bactericidal effect.
• POE alkyl ether trade names of Emulsion Japan, Inc. 102, 103, 105, 107, 110, 112, 115, 117, 503, 505, 506, 508, 510, 512, 515, 602, 603 , 605, 606, 608, 611, 615, trade names of Nikko Chemicals Co., Ltd. NIKKOL BC-2, BC-5.5, BC-7, BC-10TX, BC-15TX, BS-2, BS-4, Commercial products such as BO-2, BO-7, BO-10, and BO-15 can be used.
• the average added mole number of EO of the POE hydrogenated castor oil is 5 to 30, preferably 5 to 20. When the average added mole number of EO is less than 5 or exceeds 30, a sufficient bactericidal effect is not exhibited in the composition after storage.
• Examples of such POE hydrogenated castor oil include EMALEX HC-5, HC-7, HC-10, HC-20, HC-30 from Nippon Emulsion Co., Ltd., NIKKOL HCO-5, HCO- from Nikko Chemicals Co., Ltd. 10, commercially available products such as HCO-20 and HCO-30 can be used.
• the blending amount of the nonionic surfactant (C) is preferably 0.3 to 5%, particularly 0.5 to 2% of the whole composition. If the blending amount is less than 0.3%, a sufficient bactericidal effect may not be exhibited in the composition after storage, or the extrudability at a low temperature may not be improved. If it exceeds 5%, there may be a problem in extrudability at a low temperature, a nasty taste, or a problem in terms of storage stability.
• the dentifrice composition of the present invention in addition to the above-described constituents, other known components that are usually blended in the dentifrice composition as optional components, such as abrasives, thickeners, binders, (C) component
• abrasives thickeners
• binders binders
• Other surfactants moisturizers, sweeteners, colorants, preservatives, pH adjusters, medicinal components other than the components (A) and (B), and the like can be appropriately blended within a range not impairing the effects of the present invention. .
• abrasive silica dibasic calcium phosphate dihydrate, dibasic calcium phosphate anhydrate, calcium pyrophosphate, aluminum hydroxide, alumina, titanium dioxide, polymethyl methacrylate, insoluble calcium metaphosphate, light calcium carbonate, heavy Calcium carbonate, magnesium carbonate, tribasic magnesium phosphate, zeolite, zirconium silicate, tricalcium phosphate, hydroxyapatite, fluoroapatite, calcium deficient apatite, tricalcium phosphate, tetracalcium phosphate, eighth calcium phosphate and the like.
• the blending amount of the abrasive is preferably 10 to 50%, particularly 15 to 40% of the whole composition.
• an anionic surfactant As the surfactant, an anionic surfactant, a nonionic surfactant other than the component (C), a cationic surfactant, and an amphoteric surfactant can be blended.
• anionic surfactants include sodium alkyl sulfates such as sodium lauryl sulfate and sodium myristyl sulfate, sodium N-acyl sarcosinate such as sodium N-lauroyl sarcosinate, sodium N-myristoyl sarcosinate, sodium dodecylbenzenesulfonate, hydrogen Added coconut fatty acid monoglyceride sodium monosulfate, sodium lauryl sulfoacetate, N-acyl glutamate such as sodium N-palmitoyl glutamate, N-methyl-N-acyl taurine sodium, N-methyl-N-acylalanine sodium, ⁇ -olefin sulfone Examples include sodium acid.
• Nonionic surfactants include sugar alcohol fatty acid esters such as sorbitan fatty acid ester, polyoxyethylene sorbitan fatty acid ester, sucrose fatty acid ester, glycerin fatty acid ester, polyglycerin fatty acid ester, polyoxyethylene glycerin fatty acid ester, polyethylene glycol fatty acid Examples include polyhydric alcohol fatty acid esters such as esters, polyoxyethylene polyoxypropylene copolymers, ether-type nonionic surfactants such as polyoxyethylene alkylphenyl ether, and fatty acid alkanolamides such as lauric acid diethanolamide. .
• Examples of the cationic surfactant include alkyl ammonium and alkyl benzyl ammonium salts.
• Examples of amphoteric surfactants include betaine acetate, imidazolinium betaine, and lecithin.
• the blending amount of these surfactants is desirably in the range where the total blending amount including the nonionic surfactant of the component (C) is 0.3 to 10%, particularly 0.3 to 5%.
• sugar alcohols such as sorbit, glycerin, ethylene glycol, propylene glycol, 1,3-butylene glycol, polyethylene glycol, polypropylene glycol, xylitol, maltitol, erythritol, and polyhydric alcohols may be blended.
• the blending amount of the thickener is usually 10 to 50%, particularly 20 to 40%.
• binder sodium polyacrylate, carboxyvinyl polymer, carrageenan, sodium carboxymethyl cellulose, sodium alginate, xanthan gum, hydroxyethyl cellulose and the like can be blended.
• the amount of binder is usually 0.5-2%.
• medicinal components other than the components (A) and (B), for example, fluorides such as sodium fluoride, potassium fluoride, stannous fluoride, strontium fluoride, sodium monofluorophosphate, chlorhexidine, chloride
• fluorides such as sodium fluoride, potassium fluoride, stannous fluoride, strontium fluoride, sodium monofluorophosphate, chlorhexidine
• Antibacterial substances such as cetylpyridinium, benzalkonium chloride, benzethonium chloride, triclosan, chlorhexidine salts, sorbate, hinokitiol, azulenesulfonate, proteases such as protease, glucanase, lysozyme, tranexamic acid, epsilon aminocaproic acid, allantoin
• Anti-inflammatory substances such as glycyrrhizic acid, glycyrrhetinic acid, inorganic salts such
• saccharin sodium As sweeteners, saccharin sodium, stevioside, neohesperidyl dihydrochalcone, perilartin, p-methoxycinnamic aldehyde, aspartame and the like can be blended.
• fragrances for example, eucalyptus oil, winter green oil, cassia oil, clove oil, thyme oil, sage oil, basil oil, cardamom oil, coriander oil, peppermint oil, spearmint oil, peppermint oil, orange oil, lemon oil, mandarin oil , Lime oil, grapefruit oil, coconut oil, sweetie oil, lavender oil, rosemary oil, laurel oil, camomil oil, caraway oil, marjoram oil, celery oil, bay oil, origanum oil, pine needle oil, neroli oil, lemon Glass oil, Rose oil, Jasmine oil, Patchouli oil, Iris concrete, Rose absolute, Orange flower absolute, Vanilla absolute, Mango absolute, Patchouli absolute, Ginger oleoresin, Pepper oleoresin, Capsica Natural fragrances such as oleoresin and pepper extract, and fragrances obtained by processing these natural fragrances (front reservoir cut, rear reservoir cut, fractionated liquid extraction, essence, powder fragrance, etc.), and limonene,
• Red No. 2 Red No. 3, Red No. 225, Red No. 226, Yellow No. 4, Yellow No. 5, Yellow No. 205, Blue No. 1, Blue No. 2, Blue No. 201, Blue No. 204, Green No. 3, mica titanium, titanium oxide and the like.
• Examples of the preservative include paraoxybenzoic acid ester and sodium benzoate.
• the dentifrice composition of the present invention is preferably adjusted to pH 5 to 10, particularly 6 to 9.
• the material of the container for storing the dentifrice composition of the present invention is not particularly limited, and a container usually used for a dentifrice composition can be used. Specifically, plastic containers such as polyethylene, polypropylene, polyethylene terephthalate, and nylon can be used.
• Dentifrice compositions having the compositions shown in Tables 1 and 2 were prepared by the following production method.
• Production method (1) A phase in which (A) component: propylene glycol alginate and water-soluble components (excluding binder and propylene glycol) were mixed and dissolved at room temperature in purified water was prepared. (2) Ingredient (B) in propylene glycol: After dissolving isopropylmethylphenol, phase B was prepared in which a binder was dispersed at room temperature. (3) B phase was added and mixed in A phase under stirring, and C phase was prepared.
• component C nonionic surfactant, further components other than water-soluble components such as abrasives, and other components such as other surfactants are added, and a 1.5 L kneader is added.
• a 1.5 L kneader was added.
• Other ingredients such as abrasive silica, xanthan gum, sodium polyacrylate, sodium alginate, sorbit liquid, propylene glycol, sodium saccharin, sodium fluoride, anhydrous silicic acid, titanium oxide, sodium lauryl sulfate, and water are quasi-drugs. A material conforming to the raw material standard 2006 was used.
• the dentifrice composition thus obtained was made into a laminated tube (LDPE55 / PET12 / LDPE20 / white LDPE60 / EMAA20 / AL10 / EMAA30 / LDPE20 / LLDPE30, thickness 257 ⁇ m (Dainippon), whose innermost layer is made of linear low density polyethylene. (Printed) was filled with 50 g.
• the abbreviations and names in the layer structure of the laminated tube used are as follows, and the numbers following the abbreviations indicate the thickness ( ⁇ m) of each layer.
• LDPE Low density polyethylene white LDPE: White low density polyethylene
• LLDPE Linear low density polyethylene AL: Aluminum PET: Polyethylene terephthalate EMAA: Ethylene / methacrylic acid copolymer resin
• Periodontal disease-causing bacteria are Porphyromonas gingivalis ATCC33277 purchased from American Type Culture Collection (ATCC), and hemin and menadione Incubate to a steady state under anaerobic conditions (80 vol% nitrogen, 10 vol% carbon dioxide, 10 vol% hydrogen) at 37 ° C. with a Todd Hewitt broth culture solution (THBHM * 1 ) containing 0.15% absorbance at 550 nm. A solution suspended in PBS (manufactured by Wako Pure Chemical Industries, Ltd.) was used for the test.
• PBS manufactured by Wako Pure Chemical Industries, Ltd.
• the adherent carrier was immersed in human unstimulated saliva filtered with a 0.45 ⁇ m filter for 1 hour (37 ° C.) using a hydroxyapatite (HA) plate (manufactured by Asahi Optical Co., Ltd.) having a diameter of 7 mm and a thickness of 3.5 mm.
• HA hydroxyapatite
• the HA plate was washed three times with 1 mL of PBS, and then the adhered bacteria were dispersed by sonication (200 ⁇ A, 10 seconds) in 4 mL of PBS, and 10-fold serial dilution was performed using PBS. 50 ⁇ L of this was smeared on a blood agar plate * 2 containing 10% sheep defibrillated blood and cultured under anaerobic conditions for about 2 weeks.
• the number of Porphyromonas gingivalis adhering to the HA plate was determined from the number of grown colonies, and the number of adhering bacteria was calculated as cfu (colony forming unit) / HA plate.
• Adhesion inhibition rate (%) ((number of adherent bacteria in control ⁇ number of adherent bacteria in test composition) / (number of adherent bacteria in control)) ⁇ 100
• Adhesion inhibition effect is 80% or more ⁇ : Adhesion inhibition effect is 60% or more and less than 80% ⁇ : Adhesion inhibition effect is 40% or more and less than 60% ⁇ : Adhesion inhibition effect 0% or more and less than 40%
• THBHM Mass in 1 liter Todd Hewitt broth (Becton and Dickinson): 30 g / L Hemin (Sigma Aldrich): 5mg / L Menadion (Wako Pure Chemical Industries, Ltd.): 1mg / L Distilled water: Residue (The volume was made up so that the total amount was 1 L, and autoclaved at 121 ° C. for 20 minutes.)
• Bactericidal rate of periodontal disease bacteria is 100 or more and less than 1,000 ⁇ : Bactericidal rate of periodontal disease bacteria is 10 or more and less than 100 ⁇ : Bactericidal rate of periodontal disease bacteria is 1 or more and less than 10 ⁇ : Bactericidal rate of periodontal disease bacteria is less than 1
• Toothpaste alginate propylene glycol ester 0.15% (Product name: Kelp acid 503) Isopropyl methylphenol 0.05% Triclosan 0.02% Polyoxyethylene (6) stearyl ether 0.5% Polyoxyethylene (30) hydrogenated castor oil 1.0% 70% sorbite solution 40% Propylene glycol 3% Sodium carboxymethylcellulose 1.1% Carboxyvinyl polymer 0.2% Saccharin sodium 0.2% Sodium fluoride 0.21% Sodium lauryl sulfate 1.3% Potassium hydroxide 0.15% Silicic anhydride 22% Fragrance 1.2% Purified water balance Total 100%

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• 2011
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