WO2011115040A1 - 糖液の製造方法およびその装置 - Google Patents
糖液の製造方法およびその装置 Download PDFInfo
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- WO2011115040A1 WO2011115040A1 PCT/JP2011/055903 JP2011055903W WO2011115040A1 WO 2011115040 A1 WO2011115040 A1 WO 2011115040A1 JP 2011055903 W JP2011055903 W JP 2011055903W WO 2011115040 A1 WO2011115040 A1 WO 2011115040A1
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/18—Apparatus specially designed for the use of free, immobilized or carrier-bound enzymes
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M1/00—Apparatus for enzymology or microbiology
- C12M1/12—Apparatus for enzymology or microbiology with sterilisation, filtration or dialysis means
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/14—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus with filters, sieves or membranes
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/10—Separation or concentration of fermentation products
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
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- C—CHEMISTRY; METALLURGY
- C13—SUGAR INDUSTRY
- C13K—SACCHARIDES OBTAINED FROM NATURAL SOURCES OR BY HYDROLYSIS OF NATURALLY OCCURRING DISACCHARIDES, OLIGOSACCHARIDES OR POLYSACCHARIDES
- C13K1/00—Glucose; Glucose-containing syrups
- C13K1/02—Glucose; Glucose-containing syrups obtained by saccharification of cellulosic materials
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Definitions
- the present invention relates to a method and an apparatus for producing a sugar solution from cellulose.
- the fermentation production process of chemicals using sugar as a raw material is used for the production of various industrial raw materials.
- sugar derived from edible raw materials such as sugar cane, starch and sugar beet is used industrially as sugar for this fermentation raw material, but the price of edible raw materials will rise due to the increase in the world population in the future, or it will compete with edible foods.
- a process for producing sugar solution more efficiently than renewable non-edible resources, ie cellulose-containing biomass, or a process for efficiently converting the obtained sugar solution as a fermentation raw material into an industrial raw material The future is a future challenge.
- Non-patent Document 1 a method of producing a sugar solution by acid hydrolysis of cellulose and hemicellulose using concentrated sulfuric acid (Patent Document 1 or 2), hydrolysis of cellulose-containing biomass with dilute sulfuric acid A method for producing a sugar solution by further treatment with an enzyme such as cellulase after the treatment is disclosed (Non-patent Document 1). Further, as a method not using an acid, a method of hydrolyzing cellulose-containing biomass using subcritical water at about 250 ° C. to 500 ° C.
- Patent Document 3 a method of using cellulose-containing biomass as subcritical water
- Patent Document 4 a method for producing a sugar solution by further enzymatic treatment
- Patent Document 5 a method for producing a sugar solution
- Patent Document 6 a method for recovering and reusing the enzyme used for hydrolysis has been proposed.
- a continuous solid-liquid separation using a spin filter the obtained sugar solution is filtered through an ultrafiltration membrane, and the enzyme is recovered (Patent Document 6).
- a surfactant is added at the stage of enzyme saccharification.
- Patent Document 7 A method for suppressing enzyme adsorption and improving recovery efficiency (Patent Document 7), a method for recovering enzyme components by energizing the residue after enzymatic saccharification (Patent Document 8), and a new residue after enzymatic saccharification.
- Patent Document 9 A method of recovering and reusing an enzyme by re-introducing it into biomass
- an object of the present invention is to develop a process that exceeds the effect of reducing the amount of enzyme used in the conventional method.
- the present invention has the following configurations [1] to [11].
- [1] A method for producing a sugar solution by repeating a sugar solution production process including the following steps (1) to (3), wherein the recovered enzyme obtained in step (3) is used in the subsequent sugar solution production process
- a method for producing a sugar solution which is used in the step (1).
- (1) A step of adding a filamentous fungus-derived cellulase to cellulose and performing primary hydrolysis, (2) a step of adding an unused filamentous fungus-derived cellulase to the hydrolyzate of step (1) and performing secondary hydrolysis, and (3) solid-liquid separation of the hydrolyzate of step (2).
- the recovery of the cellulase derived from filamentous fungi in the step (3) is characterized in that the sugar solution is filtered through an ultrafiltration membrane and recovered from the non-permeation side.
- the manufacturing method of the sugar liquid in any one.
- a device for a method for producing a sugar solution including a step of hydrolyzing cellulose, a hydrolysis tank in which a recovered enzyme input pipe and an unused enzyme input pipe are connected, and an apparatus for solid-liquid separation of the hydrolyzate ,
- a sugar solution holding tank having a water supply pipe for cleaning the ultrafiltration membrane and / or removing the recovered enzyme held in the circulation pipe, and an ultrafiltration membrane device for separating the enzyme and the sugar solution,
- a device for a method for producing a sugar solution comprising a step of hydrolyzing cellulose, a cellulose / recovered enzyme mixing device and a cellulose / recovered enzyme mixture for performing primary hydrolysis by mixing recovered enzyme and cellulose Hydrolysis tank connected with supply pipe and unused enzyme inlet, apparatus for solid-liquid separation of hydrolyzate, cleaning of ultrafiltration membrane and / or water supply for removing recovered enzyme retained in circulation pipe
- An apparatus comprising, as components, a sugar liquid holding tank having piping and an ultrafiltration membrane device for separating an enzyme and a sugar liquid.
- a device comprising, in addition to the device configuration described in [9] or [10], a reverse osmosis membrane and / or a nanofiltration membrane device for further concentrating a sugar solution.
- FIG. 1 is a schematic diagram showing the procedure in the hydrolysis method of the present invention.
- FIG. 2 is a schematic diagram showing an apparatus used in the present invention.
- FIG. 3 is a schematic diagram showing an apparatus used in the present invention.
- FIG. 4 is a schematic diagram showing an apparatus used in the present invention.
- FIG. 5 is a schematic diagram showing an apparatus used in the present invention.
- FIG. 6 is a schematic diagram showing an apparatus used in the present invention.
- FIG. 7 is a schematic diagram showing an apparatus used in the present invention.
- FIG. 8 is a schematic diagram showing an apparatus used in the present invention.
- FIG. 9 is a schematic diagram showing an apparatus used in the present invention.
- FIG. 10 is a schematic diagram showing an apparatus used in the present invention.
- FIG. 11 is a diagram showing the results of SDS-PAGE of a water-insoluble Trichoderma-derived cellulase component.
- Cellulose is contained in large amounts in herbaceous biomass such as bagasse, switchgrass, napiergrass, Eliansus, corn stover, rice straw, and straw, or woody biomass such as trees and waste building materials.
- herbaceous biomass such as bagasse, switchgrass, napiergrass, Eliansus, corn stover, rice straw, and straw
- woody biomass such as trees and waste building materials.
- Cellulose-containing biomass can be preferably used as a raw material.
- the method for producing a sugar solution of the present invention When biomass-derived cellulose is used as the raw material for the sugar solution, the hydrolysis efficiency by the enzyme can be improved by pretreatment.
- the pretreatment method for cellulose-containing biomass include acid treatment, sulfuric acid treatment, dilute sulfuric acid treatment, alkali treatment, caustic soda treatment, hydrothermal treatment, subcritical water treatment, fine pulverization treatment, and steaming treatment. Alkali treatment, hydrothermal treatment or dilute sulfuric acid treatment is preferred.
- Alkali treatment includes a method of using an alkali such as sodium hydroxide, calcium hydroxide, or ammonia as the alkali, but ammonia can be particularly preferably used.
- ammonia treatment can be carried out by the methods described in JP2008-161125A and JP2008-535664A.
- the ammonia concentration to be used is added in the range of 0.1 to 15% by weight with respect to the biomass, and the treatment is performed at 4 to 200 ° C., preferably 90 to 150 ° C.
- Ammonia to be added may be in a liquid state or a gaseous state. Further, the form of addition may be pure ammonia or an aqueous ammonia solution.
- the number of processes is not particularly limited, and the process may be performed once or more. In particular, when the process is performed twice or more, the first process and the second and subsequent processes may be performed under different conditions. Since the treated product obtained by the ammonia treatment is further subjected to an enzymatic hydrolysis reaction, it is necessary to neutralize ammonia or remove ammonia. Neutralization may be performed on ammonia from which the solid content has been removed from the hydrolyzate by solid-liquid separation, or may be performed while the solid content is still contained.
- the acid reagent used for neutralization is not particularly limited. Ammonia can be removed by volatilizing ammonia into a gaseous state by keeping the ammonia-treated product in a reduced pressure state. The removed ammonia may be recovered and reused.
- hydrothermal treatment After adding water so that the cellulose-containing biomass becomes 0.1 to 50% by weight, it is treated at a temperature of 100 to 400 ° C. for 1 second to 60 minutes. By treating at such temperature conditions, hydrolysis of cellulose occurs.
- the number of processes is not particularly limited, and the process may be performed once or more. In particular, when the process is performed twice or more, the first process and the second and subsequent processes may be performed under different conditions.
- the concentration of sulfuric acid is preferably 0.1 to 15% by weight, and more preferably 0.5 to 5% by weight.
- the reaction temperature can be set in the range of 100 to 300 ° C., preferably 120 to 250 ° C.
- the reaction time can be set in the range of 1 second to 60 minutes.
- the number of processes is not particularly limited, and the process may be performed once or more. In particular, when the above process is performed twice or more, the first process and the second and subsequent processes may be performed under different conditions.
- the hydrolyzate obtained by the dilute sulfuric acid treatment contains an acid, and further needs to be neutralized in order to perform a hydrolysis reaction with cellulase or to be used as a fermentation raw material.
- the above cellulose is hydrolyzed by a filamentous fungus-derived cellulase.
- the hydrolysis of cellulose refers to the production of monosaccharides or oligosaccharides by reducing the molecular weight of cellulose by the action of cellulase.
- the reaction conditions for hydrolysis are not limited as long as the reaction conditions are the same as those for cellulase.
- the reaction temperature is preferably in the range of 15 ° C to 100 ° C, more preferably 40 ° C to 60 ° C, and more preferably 50 ° C. Is more preferable.
- the pH of hydrolysis is preferably in the range of pH 3 to 9, more preferably pH 4 to 5.5, and even more preferably pH 5.
- acid or alkali can be added and adjusted so as to have a desired pH.
- hydrolysis it is preferable to carry out stirring and mixing in order to promote contact between the cellulose and the enzyme and to make the sugar concentration of the hydrolyzate uniform. It is preferable to add water so that the solid content concentration of cellulose is in the range of 1 to 25% by weight, and more preferably in the range of 8 to 20% by weight.
- Cellulases derived from filamentous fungi include Trichoderma, Aspergillus, Cellulomonas, Clostridium, Streptomyces, Humimola, and Humimola. ), Irpex, Mucor, Talaromyces, Phanerochaete, white rot fungus, brown rot fungus, and the like.
- filamentous fungus-derived cellulases it is preferable to use trichoderma-derived cellulases having high cellulose-degrading activity.
- Trichoderma-derived cellulase is an enzyme composition containing cellulase derived from Trichoderma microorganism as a main component.
- the microorganism of the genus Trichoderma is not particularly limited, but Trichoderma reesei (Trichoderma reesei) is preferable, and specifically, Trichoderma reesei QM9414 (Trichoderma reesei QM9414), Trichoderma reesei QM9123 (Trichoderma reesei QM9123) reeseiRut C-30), Trichoderma reesei ATCC 68589 (Trichoderma reesei ATCC 68589), Trichoderma reesei PC3-7 (Trichoderma reesei PC3-7), Trichoderma reesei CL-847 (TrichodermaCL-847) 7), Trichoderma reesei MCG77 (Trichoderma reesei MCG
- the cellulase derived from a filamentous fungus used in the present invention includes an enzyme composition having a plurality of enzyme components such as cellobiohydrase, endoglucanase, exoglucanase, ⁇ -glucosidase, xylanase, and xylosidase, and has an activity of hydrolyzing and saccharifying cellulose. It is a thing.
- the filamentous fungus-derived cellulase can efficiently hydrolyze cellulose due to the concerted effect or complementary effect of a plurality of enzyme components in cellulose degradation.
- Cellobiohydrase is a general term for cellulases characterized by hydrolysis from the terminal portion of cellulose.
- the enzyme group belonging to cellobiohydrase is represented by EC number: EC3.2.1.91. Are listed.
- Endoglucanase is a general term for cellulases characterized by hydrolysis from the central part of the cellulose molecular chain.
- Exoglucanase is a general term for cellulases characterized by hydrolysis from the end of a cellulose molecular chain, and is assigned to the exoglucanase as EC numbers: EC3.2.1.74 and EC3.2.1.58. Enzyme groups are described.
- ⁇ -glucosidase is a general term for cellulases characterized by acting on cellooligosaccharide or cellobiose, and an enzyme group belonging to ⁇ -glucosidase is described as EC number: EC 3.2.1.21.
- Xylanase is a general term for cellulases characterized by acting on hemicellulose or particularly xylan, and an enzyme group belonging to xylanase is described as EC number: EC3.2.1.8.
- Xylosidase is a general term for cellulases characterized by acting on xylo-oligosaccharides, and an enzyme group belonging to xylosidase is described as EC number: EC 3.2.1.37.
- Trichoderma-derived cellulase those containing a Trichoderma microorganism culture fluid-derived component are preferably used.
- the Trichoderma-derived culture solution-derived component includes all components other than cellulase contained in the obtained culture solution after culturing in a medium adjusted so that Trichoderma microorganisms produce cellulase. That is, enzyme components other than cellulase, Trichoderma microorganisms, medium components used for culture, and the like can be exemplified.
- medium components used for culture include monosaccharides such as glucose or xylose, corn steep liquor, yeast extract, cellulase production inducers such as cellulose, minerals, vitamin components, and the like. .
- Trichoderma microorganisms may be included as a component derived from the culture solution of Trichoderma microorganisms. This is because the amount of the recovered enzyme can be increased by including Trichoderma microorganisms as a component of the Trichoderma-derived cellulase of the present invention.
- the weight ratio of each enzyme component in the cellulase derived from Trichoderma is not particularly limited.
- the culture solution derived from Trichoderma reesei contains 50 to 95% by weight of cellobiohydrase, and the rest. Endoglucanase, ⁇ -glucosidase, exo-1,4- ⁇ -D-glucosamidase, xylanase, xylosidase, endo-1,4-mannosidase, 1,2- ⁇ -mannosidase, ⁇ -glucuronidase, chitosanase, chitinase, , 4- ⁇ -glucosidase, ⁇ -galactosidase, ⁇ -galactosidase, arabinofuranosidase, xylan esterase, swollenin, hydrophobin, and the like.
- Trichoderma microorganisms produce powerful cellulase components in the culture solution, while ⁇ -glucosidase is retained in the cell or on the cell surface, so the ⁇ -glucosidase activity in the culture solution is low.
- a different or the same type of ⁇ -glucosidase may be added.
- ⁇ -glucosidase derived from Aspergillus can be preferably used. Examples of ⁇ -glucosidase derived from the genus Aspergillus include Novozyme 188 commercially available from Novozyme.
- a method for adding a heterologous or homologous ⁇ -glucosidase is to introduce a gene into a Trichoderma microorganism, to culture the Trichoderma microorganism that has been genetically modified to be produced in the culture medium,
- the method of separating may be used.
- the present invention is characterized in that the hydrolysis of cellulose by the above-mentioned cellulase derived from a filamentous fungus is carried out by dividing it into primary hydrolysis and secondary hydrolysis.
- the hydrolysis of cellulose by the above-mentioned cellulase derived from a filamentous fungus is carried out by dividing it into primary hydrolysis and secondary hydrolysis.
- the primary hydrolysis in the present invention means that hydrolysis is performed by adding a cellulase derived from a filamentous fungus to cellulose that has not been enzymatically treated.
- the enzyme used for the primary hydrolysis may be an unused enzyme, which will be described later, or a recovered enzyme. However, since the sugar production efficiency can be improved by using the recovered enzyme, the recovered enzyme is preferably used.
- the recovered enzyme contains an enzyme component partially modified by the heat during hydrolysis. Adsorption to the adsorption points existing on the surface is particularly strong, and as a result, non-specific adsorption is performed on adsorbing surface sites such as lignin in cellulose.
- the recovered enzyme of the present invention is characterized in that the xylan decomposition activity is increased as the number of times of recovery is increased.
- the xylan decomposition activity contained in the recovered enzyme can be measured by using a reagent xylan such as birch wood xylan as a decomposition substrate.
- Cellulase components derived from filamentous fungi that are involved in xylan degradation activity include xylanase and xylosidase.
- xylanases there are genes of xyn1 (GH11), xyn2 (GH11), xyn3 (GH10), xyn4 (GH5), xyn5b (GH5), and xyn11 (GH11).
- xylosidase there are genes bxl1 / bxl3a (GH3), bxl3b (GH3), and bxl3c (GH3).
- the above-described genes encode xylanase and xylosidase, respectively, and are included as cellulase components derived from filamentous fungi.
- Xylanase 3 (molecular weight 38 kDa, xyn3), endo- ⁇ -1,4-xylanase (molecular weight 25 kDa, xyn1), ⁇ -xylosidase (molecular weight 88 kDa, bxl1 / bxl3a) are particularly exemplified as xylan-degrading enzymes that are enhanced as recovered enzymes. it can.
- the xylan components surrounding the cellulose are preferentially hydrolyzed, and the sugar productivity in the primary and secondary hydrolysis can be increased.
- the reaction time for primary hydrolysis is preferably in the range of 15 minutes to 6 hours. When the time is less than 15 minutes, the degree of improvement in the sugar production efficiency may be low. On the other hand, when the time is 6 hours or longer, the sugar production efficiency with respect to time may be low.
- the cellulose concentration, reaction temperature, and pH are not particularly limited, and the above hydrolysis conditions can be preferably applied.
- the amount of enzyme added in the primary hydrolysis is preferably 1/1000 to 1/50 of the weight of the cellulose pretreatment product.
- the weight of the cellulose pretreatment product can be calculated by measuring the weight of the solid content contained in the cellulose pretreatment product. The weight of such solid content is determined by separating the water-soluble compound by performing solid-liquid separation and water washing by a method such as centrifugation and membrane filtration. It can be calculated by drying until it becomes, and measuring its weight.
- the enzyme addition amount can be calculated by measuring the protein concentration in a solution containing an unused enzyme by the BCA method and multiplying by the solution amount of the unused enzyme to which this protein concentration is added.
- the primary hydrolyzate obtained by primary hydrolysis monosaccharide components generated by hydrolysis are accumulated.
- xylan decomposition activity tends to increase. That is, the primary hydrolyzate when the recovered enzyme is used for primary hydrolysis has a feature that a large amount of xylose is produced.
- the primary hydrolyzate obtained by the primary hydrolysis of the present invention can be subjected to the secondary hydrolysis described below after performing an operation to increase the concentration of the undecomposed solid matter as it is or as a solid-liquid separation. .
- separation can be utilized as a sugar liquid.
- the secondary hydrolysis in the present invention means that hydrolysis is performed by further adding an unused enzyme to the hydrolyzate obtained by the primary hydrolysis described above. No special solid-liquid separation operation is required for the primary hydrolyzate. If necessary, water may be further added, but is not particularly limited.
- an unused enzyme is charged and used for secondary hydrolysis.
- the amount of enzyme introduced in primary hydrolysis (unused enzyme or recovered enzyme) is not sufficient for cellulose degradation.
- the purpose is that sugar production efficiency and enzyme recovery efficiency can be improved by introducing an unused enzyme in two steps of primary hydrolysis and secondary hydrolysis.
- the amount of sugar production after the second time decreases, which is not preferable. Therefore, in addition to the recovered enzyme, in the second hydrolysis, by introducing an unused enzyme, production equivalent to the first or previous sugar production can be performed. That is, in the method for producing the sugar solution, the production of a sugar concentration above a certain level can be repeated.
- the addition of the unused enzyme in the secondary hydrolysis may be performed in a plurality of times (divided input). For example, after completion of the primary hydrolysis, half of the amount of unused enzyme to be added in the secondary hydrolysis is added, and after hydrolysis for several hours, the remaining half is further added. In the present invention, even if the novel enzyme is divided and added several times in the secondary hydrolysis as described above, it is called secondary hydrolysis including these operations.
- the reaction time for secondary hydrolysis is preferably longer than that for primary hydrolysis. Further, the specific secondary hydrolysis reaction time is preferably in the range of 1 to 200 hours, more preferably in the range of 6 to 72 hours, and still more preferably in the range of 12 to 24 hours.
- the reaction time is a matter that should be adjusted depending on the amount of enzyme used, the reaction temperature, the target sugar concentration, etc., but considering the recovery and reuse of cellulase, if the reaction time exceeds 200 hours, Cellulase thermal inactivation may occur. On the other hand, if the reaction time is less than 1 hour, a hydrolyzate having a sufficient sugar concentration may not be obtained.
- the amount of enzyme added in the secondary hydrolysis is preferably 1/1000 to 1/50 of the weight of the cellulose pretreatment product.
- the weight of the cellulose pretreatment product can be calculated by the weight of the solid content of the cellulose pretreatment product before the primary hydrolysis.
- the relational expression of enzyme addition amount in primary hydrolysis> enzyme addition amount in secondary hydrolysis is satisfied.
- the amount added here can be calculated by multiplying the protein concentration of the unused enzyme or the recovered enzyme to be added by the amount of the enzyme solution to be added.
- the protein concentration can be measured by the above-mentioned known methods to calculate the protein concentration of the recovered enzyme and the unused enzyme.
- the protein concentration here is simply a protein concentration, and it is not limited whether it is a cellulase-derived component or other component. In the present invention, when the relational expression of the addition amount is satisfied, a larger amount of sugar production can be obtained, and the enzyme recovery efficiency can be further improved.
- the present invention also includes a step of solid-liquid separation of the secondary hydrolyzate, recovering the filamentous fungus-derived cellulase from the resulting sugar solution, and reusing the recovered filamentous fungus-derived cellulase for the primary hydrolysis. It is characterized by. Hereinafter, it demonstrates in order of a process.
- the solid-liquid separation of the secondary hydrolyzate is performed for the purpose of separating the sugar liquid obtained from the secondary hydrolysis and the hydrolysis residue.
- the sugar solution refers to a sugar solution obtained by hydrolysis of cellulose described above.
- sugars are classified according to the degree of polymerization of monosaccharides, such as monosaccharides such as glucose and xylose, oligosaccharides obtained by dehydration condensation of 2 to 9 monosaccharides, and polysaccharides obtained by dehydration condensation of 10 or more monosaccharides. Classified as a saccharide.
- the sugar solution obtained according to the present invention contains glucose or xylose as a main component, and also contains oligosaccharides such as cellobiose and monosaccharides such as arabinose and mannose, although in small amounts.
- oligosaccharides and polysaccharides dissolved in water HPLC can be used for quantification by comparison with a standard product.
- the solid-liquid separation method is not particularly limited, but solid-liquid separation can be performed by a centrifugal method such as a screw decanter, a filtration method such as a filter press, or a membrane separation method such as a microfiltration membrane.
- filamentous fungus-derived cellulase exists in the secondary hydrolyzate in either a dissolved state in a sugar solution or adsorbed to an undegraded solid residue. It can be recovered from the sugar solution side by separation.
- a method for recovering the cellulase derived from the filamentous fungus from the sugar solution a method in which the sugar solution is filtered through an ultrafiltration membrane and the cellulase is recovered from the non-permeating side can be mentioned as a preferred example.
- an ultrafiltration membrane to be used a membrane made of materials such as polyethersulfone (PES), polyvinylidene fluoride (PVDF), and regenerated cellulose can be used.
- regenerated cellulose is subject to decomposition by cellulase. It is preferable to use an ultrafiltration membrane made of a synthetic polymer such as PES or PVDF.
- the fractionation molecular weight of the ultrafiltration membrane is not particularly limited as long as the cellulase used can be efficiently recovered, but an ultrafiltration membrane having a fractionation molecular weight in the range of 1,000 to 50,000 is preferable.
- the amount of the recovered enzyme is not particularly limited because the amount of the recovered enzyme varies depending on the amount of the unused enzyme added to the secondary hydrolysis.
- a cellulase component derived from a water-insoluble filamentous fungus may be obtained as a collected enzyme component, particularly in the process of separating the collected enzyme using an ultrafiltration membrane.
- a water-insoluble filamentous fungus-derived cellulase component is an enzyme component produced in the process of hydrolysis, or in the process of enzyme recovery by an ultrafiltration membrane or the like.
- Such a water-insoluble filamentous fungus-derived cellulase component is preferably used as it is as a recovered enzyme component without being removed by solid-liquid separation, filtration or the like.
- the cellulase component derived from a water-insoluble filamentous fungus is mainly composed of cellobiohydrase.
- Water-insoluble means that it exists in the recovered enzyme solution in the form of precipitates, flocs, pellets, and fine particles, and the recovered enzyme is placed in a tube and centrifuged to give a cellulase component derived from water-insoluble filamentous fungi. As a precipitate.
- the cellulase component derived from water-insoluble filamentous fungi recovered as a precipitate can be confirmed by colors such as white, pale yellow, and brown. A part of the cellulase component derived from water-insoluble filamentous fungi can be separated and resuspended in water to partially dissolve it.
- the filamentous fungus-derived cellulase recovered from the secondary hydrolyzate (hereinafter referred to as recovered enzyme) is reused for primary hydrolysis.
- recovered enzyme The filamentous fungus-derived cellulase recovered from the secondary hydrolyzate
- the advantages of using the recovered enzyme in the primary hydrolysis are as described above. There is no particular limitation on the number of times the recovered enzyme is reused.
- the amount of the enzyme added when the enzyme recovered from the secondary hydrolyzate is reused for the primary hydrolysis is larger than the amount of the unused enzyme added in the secondary hydrolysis.
- the amount of enzyme added here is measured by the amount of protein as described above.
- the cellulase activity of the recovered enzyme enzyme activity relative to the amount of protein
- the amount of recovered enzyme to be reused for primary hydrolysis > the amount of secondary hydrolysis
- the sugar production efficiency with respect to the amount of the unused enzyme increases.
- the sugar solution obtained according to the present invention contains monosaccharides such as glucose, xylose, arabinose and manose derived from cellulose and hemicellulose (xylan and arabinan).
- the proportion of monosaccharides is not particularly limited, but the main monosaccharide components are glucose and xylose.
- the sugar solution of the present invention may contain an oligosaccharide such as cellobiose, although the amount is smaller than that of a monosaccharide.
- the concentration of monosaccharides contained in the sugar solution is not particularly limited, but is preferably 0.1 to 20% by weight, more preferably 5 to 20% by weight. When the concentration of the sugar solution is in the range of 5 to 20% by weight, the sugar solution can be used as a fermentation raw material for microorganisms without the need for special concentration.
- the sugar solution of the present invention can be concentrated with a nanofiltration membrane and / or a reverse osmosis membrane.
- a polymer material such as cellulose acetate polymer, polyamide, polyester, polyimide, vinyl polymer, polysulfone can be used. It is not limited to the film
- the nanofiltration membrane used in the present invention is preferably a spiral membrane element.
- preferable nanofiltration membrane elements include, for example, GE Osmonics GEsepa, which is a cellulose acetate-based nanofiltration membrane element, Alfa Laval nanofiltration membrane element NF99 or NF99HF having polyamide as a functional layer, and crosslinked piperazine Nano-filtration membrane element NF-45, NF-90, NF-200, NF-270 or NF-400 made by FILM TEC with polyamide as a functional layer, or nanofiltration made by Toray Industries, Inc., which is mainly composed of crosslinked piperazine polyamide
- the company's nanofiltration membrane element SU-210, SU-220, SU-600 or SU-610, including the membrane UTC60 may be mentioned, more preferably NF99 or NF99HF, NF-45, NF-90, NF-200 or NF -400, yes SU-210, SU-220, a SU-600 or SU
- the reverse osmosis membrane used in the present invention is preferably a spiral membrane element as in the case of the nanofiltration membrane.
- preferable reverse osmosis membrane elements include, for example, low pressure type SU-710, SU-720, SU-720F, SU-710L, SU-720L, SU-, which are polyamide-based reverse osmosis membrane modules manufactured by Toray Industries, Inc.
- the apparatus of the present invention is an apparatus mechanism for carrying out the above-described method for producing a sugar solution as follows: 1. Hydrolysis tank connected with recovered enzyme input pipe and unused enzyme input pipe; 2. an apparatus for solid-liquid separation of the hydrolyzate; 3. A sugar solution holding tank having a water supply pipe for washing the ultrafiltration membrane and / or removing the recovered enzyme held in the circulation pipe. It is an apparatus configuration in which an ultrafiltration membrane apparatus for separating an enzyme and a sugar solution is functionally connected. That is, the method for producing a sugar liquid according to the present invention is characterized by performing primary hydrolysis using a recovered enzyme. To do this, 1. A hydrolysis tank to which a recovered enzyme input pipe and an unused saccharifying enzyme input pipe were connected was installed.
- FIG. 2 is an example of an apparatus for carrying out the method of the present invention. That is, the apparatus of FIG. 2 can independently input the recovered enzyme into the hydrolysis tank, or can further input the recovered enzyme input pipe 4 that can control the input, and the unused enzyme independently into the hydrolysis tank.
- the apparatus includes a sugar solution holding tank 12 having a water supply pipe 11 for removing the retained recovered enzyme, and an ultrafiltration membrane device 14 for separating the enzyme and the sugar solution.
- the hydrolysis tank 1 was further provided with a heat retaining equipment 2 for maintaining the hydrolysis temperature, a stirring blade 3 for stirring and mixing lignocellulose, and a cellulose inlet 7.
- the recovered enzyme input pipe 4 and the unused enzyme input pipe 6 are connected to the recovered enzyme holding tank 5 and the unused enzyme holding tank 8 through valves, respectively.
- the valve is preferably electronically controlled separately by a pinch valve.
- the press filtration device 9 that separates hydrolysis from the hydrolysis tank 1 described above is connected via a valve and an air pump, and the hydrolyzate is fed into the press filtration device 9.
- the press filtration device 9 is connected to a compressor 10 for supplying filtration pressure.
- the sugar liquid obtained by press filtration is held in the sugar liquid holding tank 12.
- the sugar liquid holding tank 12 is connected to the ultrafiltration membrane device 14 via a circulation pump 13.
- the recovered enzyme that has passed through the membrane side (non-permeate side) of the ultrafiltration membrane returns to the sugar solution holding tank 12 via the circulation pipe 15.
- the sugar solution from which the enzyme has been removed is recovered as a filtrate on the secondary side (permeation side).
- the recovered enzyme recovered in the sugar liquid holding tank 12 is sent to the recovered enzyme holding tank 5 through the recovered enzyme pipe 16 and the pump. Water is supplied from the water supply pipe 11 to the sugar solution holding tank 12, and this water is held on the surface of the ultrafiltration membrane or in the circulation pipe 15 by circulating the ultrafiltration membrane device 14 and the circulation pipe 15 with the circulation pump 13.
- the recovered enzyme component thus collected can be further recovered as a solution, which is efficient. Moreover, the water-insoluble filamentous fungus-derived cellulase component adhering to the ultrafiltration membrane surface or the like can also be recovered. Furthermore, the circulation of water can also clean the surface of the ultrafiltration membrane provided in the ultrafiltration membrane device 14 and is useful for suppressing membrane fouling. Further, the water held in the sugar solution holding tank 12 by this operation is sent to the collected enzyme holding tank 5 through the collected enzyme pipe 16. Therefore, the water supplied from the water supply pipe 11 is used for the hydrolysis of lignocellulose in the hydrolysis tank 1.
- FIG. 3 is another example of an apparatus for carrying out the method of the present invention. That is, in the apparatus shown in FIG. 3, the cellulose / recovered enzyme mixing device 18, the cellulose / recovered enzyme mixture input pipe 17 and the unused enzyme input pipe 6 for mixing the recovered enzyme and cellulose and performing primary hydrolysis are independent.
- a hydrolyzed tank 1 a press filtration device 9 for solid-liquid separation of the hydrolyzate, and a water supply pipe 11 for cleaning the ultrafiltration membrane and / or removing the recovered enzyme retained in the circulation pipe 15.
- the apparatus includes a sugar liquid holding tank 12 and an ultrafiltration membrane device 14 for separating an enzyme and a sugar liquid as components.
- the difference from the apparatus of FIG. 2 is a cellulose / recovery enzyme mixing apparatus 18 and its supply port 17.
- the cellulose / recovered enzyme mixing device 18 is a device for mixing cellulose and recovered enzyme, and the recovered enzyme and cellulose are stirred and mixed by an internal screw.
- the primary hydrolysis of the step (1) is performed.
- the cellulose / recovery enzyme mixing device 18 may be kept at a temperature suitable for primary hydrolysis.
- the recovered enzyme may be kept warm and mixed with cellulose in the cellulose / recovery enzyme mixing device 18 for primary hydrolysis.
- the power of stirring and mixing in the hydrolysis tank 1 can be reduced by mixing the recovered enzyme and cellulose in advance.
- the time required for uniform dispersion of cellulose in the hydrolysis tank 1 can be shortened, resulting in hydrolysis time. Can be further shortened.
- the primary hydrolyzate obtained from the cellulose / recovered enzyme mixing device 18 is introduced into the hydrolyzing device 1 from the cellulose / recovered enzyme mixture supply pipe 17. Thereafter, the unused filamentous fungus-derived cellulase-containing enzyme in step (2) is added from the unused enzyme charging pipe 6 to perform secondary hydrolysis. Subsequent solid-liquid separation and enzyme recovery operation are the same as those in the apparatus of FIG.
- FIG. 4 shows another example of an apparatus for carrying out the method of the present invention.
- the apparatus of FIG. 4 is an apparatus in the case of installing a solid-liquid separator 19 including a filter press with respect to the apparatus of FIG. Further, the recovered enzyme holding tank 5, the unused enzyme holding tank 8, and the stirring blade 3 shown in FIG. 2 are not shown in FIG. 4 because they may be installed as necessary.
- the solid matter separated by the solid-liquid separator 19 is removed from the solid matter discharge pipe 20.
- the solid-liquid separation device 19 may be a filter press as shown in FIGS. 2 and 3, and other solid-liquid separation devices include a continuous centrifuge, a screw decanter, a DeLaval centrifuge, a screw press, a belt filter, A drum filter can be exemplified.
- the basic features of the apparatus are that the recovered enzyme input pipe 4 and the unused enzyme input pipe 6 are independently connected to the hydrolysis tank, and the addition of the recovered enzyme and the addition of the unused enzyme can be controlled independently, and the water supply pipe 11 Is connected to the sugar solution holding tank 12 so that water supplied from the water supply pipe 11 circulates through the ultrafiltration membrane device 14 and can be supplied to the hydrolysis tank 1 through the recovery enzyme pipe 16. This is common to the apparatus shown in FIGS.
- FIG. 5 is another example of an apparatus for carrying out the method of the present invention.
- the apparatus of FIG. 5 is basically the same as the apparatus of FIG. 4 described above, but is an apparatus in which the non-permeated liquid of the ultrafiltration membrane 14 is connected to the recovered enzyme holding tank 5.
- this is a device in which a spiral element is used as an ultrafiltration membrane, and these are connected in series or in a tree connection.
- the water supply pipe 11 is connected to the sugar solution holding tank 12, and the water supplied from the water supply pipe 11 is limited by switching the pipe with the three-way valve 21. After circulating the outer filtration membrane device 14 and switching the three-way valve 21, the water is supplied to the recovered enzyme holding tank 5.
- the recovered enzyme holding tank 5 is connected to a recovered enzyme pipe 16 through which the supply can be supplied to the hydrolysis tank 1.
- the recovered enzyme input pipe 4 and the unused enzyme input pipe 6 are independently connected to the hydrolysis tank, and the addition of the recovered enzyme and the addition of the unused enzyme can be controlled independently, as in the apparatus shown in FIGS. .
- FIG. 6 is another example of an apparatus for performing the method of the present invention.
- FIG. 6 shows a device in which a microfiltration membrane device 22 is further installed in the subsequent stage of the solid-liquid separation device 19.
- a liquid containing no solids can be obtained almost completely by processing with the microfiltration membrane device 22. Thereby, the membrane fouling of the back
- FIG. 7 is a detailed drawing of the microfiltration membrane device 22 of FIG. 6 and shows a device configuration for carrying out cross flow filtration. This is a device that holds the filtrate separated by the solid-liquid separation device 19 in a solid-liquid separation filtrate tank 23 and crossflow-filters the microfiltration membrane 25 via a pump 24.
- the microfiltration membrane 25 may be a flat membrane or a hollow fiber membrane.
- the hollow fiber membrane may be either an internal pressure type or an external pressure type.
- FIG. 8 is a detailed drawing of the microfiltration membrane device 22 of FIG. 6, and is a diagram showing a device configuration for performing dead-end filtration in the microfiltration membrane device 22.
- the filtrate separated by the solid-liquid separator 19 is held in a solid-liquid separation filtrate holding tank 23 and filtered through a microfiltration membrane 25.
- a pressure air supply device 26 for performing bubble cleaning on the membrane surface may be provided, or a reverse cleaning pump 27 for back cleaning may be provided.
- the backwashing may be a filtrate collected in the sugar solution holding tank 12 or may be a general membrane washing water and a chemical solution.
- the microfiltration membrane 25 may be a flat membrane or a hollow fiber membrane.
- the hollow fiber membrane may be either an internal pressure type or an external pressure type.
- FIG. 9 is another example of an apparatus for performing the method of the present invention.
- the apparatus for producing a sugar liquid of the present invention may be an apparatus further comprising a reverse osmosis membrane and / or a nanofiltration membrane for concentrating the sugar liquid.
- FIG. 9 shows a device in which a nanofiltration membrane or reverse osmosis membrane device 30 is connected to the device of FIG.
- a sugar liquid concentration tank 28 is further connected to the filtrate side of the ultrafiltration membrane device 14, and is filtered through a reverse osmosis membrane and / or a nanofiltration membrane 30 via a high-pressure pump 29. Since the sugar solution is blocked by the reverse osmosis membrane and / or the nanofiltration membrane, it is concentrated in the sugar solution concentration tank 28. On the other hand, excess water can be removed as filtrate.
- the reverse osmosis membrane and / or nanofiltration membrane device 30 can be installed by connecting to the filtrate side of the ultrafiltration membrane device 14 in any of the devices shown in FIGS.
- FIG. 10 is another example of an apparatus for performing the method of the present invention.
- the recovered enzyme input pipe 4 and the unused enzyme input pipe 6 are preferably independently connected to the hydrolysis tank 1, but the above-mentioned pipe 4 and pipe 6 can control the input of the respective enzyme components. If present, it may be connected to the hydrolysis tank 1 as one pipe (unused enzyme or recovered enzyme input pipe) after being joined and connected by the three-way valve 31 or the like.
- the water supplied from the water supply pipe 11 may be hot water.
- the temperature of the hot water is preferably 60 ° C. or lower in consideration of enzyme deactivation. Further, by using warm water supplied from the water supply pipe, when the inside of the ultrafiltration membrane device 14 is circulated, a high cleaning effect of the ultrafiltration membrane can be obtained.
- the cleaning effect is preferably such that the temperature of the hot water is 30 ° C. to 60 ° C.
- Trichoderma-derived cellulase 1/100 amount of ⁇ -glucosidase (Novozyme 188) as a protein weight ratio was added to the culture solution adjusted under the above-mentioned conditions, and this was used as a Trichoderma-derived cellulase in the following examples.
- the enzyme activity of Trichoderma-derived cellulase was measured by the following procedure. 1) Crystalline cellulose decomposition activity To the enzyme solution (adjusted under predetermined conditions), Avicel (Merck, Inc., required confirmation) was added to 1 g / L, and sodium acetate buffer (pH 5.0) to 100 mM, The reaction was carried out at 50 ° C. for 24 hours. The reaction solution was adjusted with a 1 mL tube, and the reaction was carried out while rotating and mixing under the above conditions. After the reaction, the tube was centrifuged, and the glucose concentration of the supernatant component was measured. The glucose concentration was measured according to the method described in Reference Example 3. For the Avicel degradation activity, the generated glucose concentration (g / L) was used as the activity value as it was.
- Cellobiose decomposition activity Cellobiose (Wako Pure Chemical Industries) 500 mg / L and sodium acetate buffer solution (pH 5.0) were added to the enzyme solution so as to have a concentration of 100 mM, and reacted at 50 ° C. for 0.5 hour.
- the reaction solution was adjusted with a 1 mL tube, and the reaction was carried out while rotating and mixing under the above conditions. After the reaction, the tube was centrifuged, and the glucose concentration of the supernatant component was measured. The glucose concentration was measured according to the method described in Reference Example 3.
- the cellobiose decomposition activity was determined by directly using the generated glucose concentration (g / L) as an activity value.
- xylan degradation activity To the enzyme solution, 10 g / L of xylan (Birch wood xylan, Wako Pure Chemical Industries) and sodium acetate buffer (pH 5.0) were added to a concentration of 100 mM and reacted at 50 ° C for 4 hours. . The reaction solution was adjusted with a 1 mL tube, and the reaction was carried out while rotating and mixing under the above conditions. After the reaction, the tube was centrifuged, and the xylose concentration of the supernatant component was measured. The xylose concentration was measured according to the method described in Reference Example 3. For the xylose decomposition activity, the generated xylose concentration (g / L) was directly used as the activity value.
- Comparative Example 1 a sugar solution was produced from cellulose without performing primary hydrolysis and secondary hydrolysis.
- Step 1 Hydrolysis
- the solution was mixed and added. Distilled water was further added so that the weight of the added solution was 10 g.
- This composition was transferred to a branched reaction vessel (Tokyo Rika Co., Ltd., ⁇ 30 NS14 / 23), and incubated at 50 ° C. for 19 hours and stirred for hydrolysis (Tokyo Rika Co., Ltd .: small mechanical stirrer CPS-1000, conversion).
- Step 2 Solid-liquid separation and enzyme recovery from sugar solution (recovered enzyme)
- the hydrolyzate of step 1 was subjected to solid-liquid separation by centrifugation (4500 G, 10 minutes), and separated into a sugar solution and a residue.
- the glucose and xylose concentrations in the sugar solution were measured by the method described in Reference Example 3 and calculated as the product sugar.
- the sugar solution was subjected to membrane filtration (Millipore's Steriflip-GP material: PES), and the obtained supernatant was subjected to ultrafiltration membrane with a molecular weight cut off of 10,000 (Sartorius Stedim biotech VIVASPIN 20, material: PES). Utilized and centrifuged at 4500 G until the membrane fraction was 1 mL. Distilled water (10 mL) was added to the membrane fraction and centrifuged again at 4500 G until the membrane fraction reached 1 mL. Thereafter, the enzyme was recovered from the membrane fraction and used as the recovered enzyme. The recovered enzyme was reused in the hydrolysis in Step 1 described above.
- membrane filtration Micropore's Steriflip-GP material: PES
- cellulase was recovered and reused by circulating steps 1 and 2.
- the steps 1 and 2 were set as a set, and collection and reuse were performed a total of 6 times.
- the 0th reaction without recovery and reuse was performed according to the following procedure.
- Step 0: 0th hydrolysis 0.3 mL of an unused enzyme (protein concentration: 50 mg / mL) (protein amount: 15 mg) was added to cellulose pretreated materials 1 to 4 (1 g each) (the recovery enzyme was not added since it was the 0th time). Distilled water was further added so that the weight of the added solution was 10 g.
- This composition was transferred to a branched reaction vessel (Tokyo Rika Co., Ltd., ⁇ 30 NS14 / 23), and incubated at 50 ° C. for 19 hours and stirred for hydrolysis (Tokyo Rika Co., Ltd .: small mechanical stirrer CPS-1000, conversion). Adapter, addition port with three-way cock, thermal insulation device MG-2200). The obtained hydrolyzate was separated by the method described in Step 2 above to obtain a recovered enzyme. At this time, the glucose and xylose concentrations of the sugar solution were measured.
- Table 1 shows that the glucose concentration (Glc, g / L) and the xylose concentration (Xly, g) of the sugar solution obtained by each reaction when Steps 0 and 2 were performed once and Steps 1 and 2 were performed in order six times in total. / L) is summarized in Table 1.
- Glucose (Glc) and xylose (Xyl) decreased with the number of recovery and reuse. It was also found that the sugar production efficiency gradually decreased with the number of reuses (N).
- Example 1 Hereinafter, as an example, a sugar solution was produced by performing primary hydrolysis and secondary hydrolysis of cellulose.
- Step 1 Primary hydrolysis
- Distilled water was added to the cellulose pretreated materials 1 to 4 (1 g each), the recovered enzyme recovered in the procedure of Step 3 described later was added, and distilled water was further added so that the total weight became 10 g.
- This composition was transferred to a branching test tube (Tokyo Rika Co., Ltd., ⁇ 30 NS14 / 23), this composition was transferred to a branching reaction vessel (Tokyo Rika Co., Ltd., ⁇ 30 NS14 / 23), and kept at 50 ° C. for 1 hour.
- the mixture was stirred and hydrolyzed (manufactured by Tokyo Rika Co., Ltd .: small mechanical stirrer CPS-1000, conversion adapter, addition port with three-way cock, heat retaining device MG-2200).
- Step 2 Secondary hydrolysis
- Step 3 Solid-liquid separation and enzyme recovery from sugar liquid (recovered enzyme)
- the secondary hydrolyzate of Step 3 was subjected to solid-liquid separation by centrifugation (4500 G, 10 minutes), and separated into a sugar solution and a residue.
- the glucose and xylose concentrations in the sugar solution were measured by the method described in Reference Example 3 and calculated as the N-th produced sugar.
- the sugar solution was subjected to membrane filtration (Sterriflip-GP manufactured by Millipore, material: PES), and the obtained supernatant was an ultrafiltration membrane having a molecular weight cut off of 10,000 (VIVASPIN 20, manufactured by Sartorius Stedim Biotech, material: PES). And centrifuged at 4500 G until the membrane fraction reached 1 mL.
- Distilled water (10 mL) was added to the membrane fraction and centrifuged again at 4500 G until the membrane fraction reached 1 mL. Thereafter, the enzyme was recovered from the membrane fraction and used as the recovered enzyme. The recovered enzyme was reused in the hydrolysis in Step 1 described above.
- cellulase was recovered and reused by circulating steps 1 to 3.
- the steps 1 to 3 were set as a set, and collection and reuse were performed 6 times in total.
- the 0th reaction without recovery and reuse was performed according to the following procedure.
- Step 0: 0th hydrolysis 0.3 mL of an unused enzyme (protein concentration: 50 mg / mL) (protein amount: 15 mg) was added to cellulose pretreated materials 1 to 4 (1 g each) (the recovery enzyme was not added since it was the 0th time). Distilled water was further added so that the weight of the added solution was 10 g.
- This composition was transferred to a branched reaction vessel (Tokyo Rika Co., Ltd., ⁇ 30 NS14 / 23), and incubated at 50 ° C. for 19 hours and stirred for hydrolysis (Tokyo Rika Co., Ltd .: small mechanical stirrer CPS-1000, conversion). Adapter, addition port with three-way cock, thermal insulation device MG-2200). The obtained hydrolyzate was separated by the method described in Step 3 above to obtain a recovered enzyme. At this time, the glucose and xylose concentrations of the sugar solution were measured.
- Table 2 shows that the glucose concentration (Glc) (g / L) and xylose concentration (Xly) of the sugar solution obtained by each reaction when Steps 0 and 3 were performed once and Steps 1 to 3 were performed in total six times in order.
- Table 2 summarizes (g / L). Although glucose (Glc) and xylose (Xyl) decreased with the number of times of recovery and reuse, it was confirmed that the amount of sugar produced gradually increased as compared with Comparative Example 1 (Table 1).
- Example 2 Measurement of added amount of recovered enzyme in primary hydrolysis
- the protein concentration of recovered enzyme added to primary hydrolysis was measured using a BCA measurement kit (BCA Protein Assay Reagent Kit, Pierce). The absorbance was measured at 562 nm using bovine albumin (2 mg / mL) as a sample, and the colorimetric determination was performed.
- Table 3 summarizes the relationship between the added amount of the recovered enzyme and the added amount of the unused enzyme with respect to the cellulose pre-treated product 2 that was recovered and reused and was recovered N times.
- Example 1 Compared with the amount of glucose produced in Table 2, recovery, reuse, and recovery for the fourth and subsequent times, that is, the amount of enzyme added in primary hydrolysis> the amount of enzyme added in secondary hydrolysis, and further reused in primary hydrolysis It was confirmed in this example that the amount of glucose produced was further improved when the relationship of the amount of recovered enzyme to be added> the amount of unused enzyme added in secondary hydrolysis was established.
- Example 3 Enzyme activity of recovered enzyme With respect to cellulose pretreated product 3, recovered enzyme activity (Comparative Example 1: When recovered enzyme and new enzyme were simultaneously added, Example 1: recovered enzyme was added to perform primary hydrolysis. Then, the new enzyme was added).
- the enzyme activity was measured in accordance with Reference Example 3 and divided into three types of decomposition activities: 1) crystalline cellulose decomposition activity, 2) cellobiose decomposition activity, and 3) xylan decomposition activity. Regarding each degradation activity, the enzyme activity in the unused enzyme (10 mg) was shown as 100 (%), and the enzyme activity in the recovered enzyme was shown as a relative value (%).
- Table 4 (Example 1) and Table 5 (Comparative Example 1) show the recovered enzyme activities for the second and fourth recovery times.
- the crystalline cellulose decomposition activity, cellobiose decomposition activity, and xylan decomposition activity tend to increase, but in particular, the xylan decomposition activity has increased.
- the xylan decomposition activity is particularly concerned with xylanase and xylosidase derived from Trichoderma, and it is considered that the recovery efficiency thereof increased with the number of times.
- Aggregated trichoderma-derived cellulase component contained in the recovered enzyme It was found that after the fourth recovery, a water-insoluble component was generated in the recovered enzyme component recovered as the non-permeate of the ultrafiltration membrane. .
- the water-insoluble Trichoderma-derived cellulase component was analyzed by the following procedure.
- the cellulose pretreatment product 3 was subjected to primary hydrolysis and secondary hydrolysis according to the procedure of Example 1, and the recovered enzyme component with the fourth recovery count was analyzed.
- the recovered enzyme 100 ⁇ L was placed in a 1.5 mL centrifuge tube and centrifuged at 15000 rpm for 5 minutes. After centrifugation, the supernatant was removed and a pellet was obtained at the bottom of the tube. 100 ⁇ L of pure was added, and the pellet was washed, and then a sample preparation buffer (Ez Apply, ATTO) was added to perform SDS-PAGE (e-PAGE, 15% gel concentration, ATTO). Staining was performed with Coomassie Brilliant Blue (BioSafecoomastain, BioRAD). In order to measure the molecular weight, a molecular weight marker (PrecisionPlus Protein Standard, Kaleidoscope, BioRAD) was used.
- Example 5 Effect of water-insoluble Trichoderma-derived cellulase component as a recovered enzyme component
- Step 3 of Example 1 cellulose pretreated product 3
- the recovered enzyme was centrifuged at 15000 rpm for 5 minutes. Only the obtained supernatant solution was used as a recovered enzyme, and the amount of sugar produced when it was reused was compared with the result of Example 1. That is, in Example 5, the recovered enzyme is reused when the water-insoluble Trichoderma-derived cellulase component contained in the recovered enzyme is removed.
- a sugar solution can be efficiently produced from cellulose, and the obtained sugar solution can be used as a sugar raw material for various fermentation products.
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Abstract
Description
[1]以下の工程(1)~(3)を含む糖液製造プロセスを繰り返して糖液を製造する方法であって、工程(3)で得られた回収酵素を次回以降の糖液製造プロセスの工程(1)に使用することを特徴とする、糖液の製造方法。
(1)セルロースに糸状菌由来セルラーゼを添加し一次加水分解する工程、
(2)工程(1)の加水分解物に未使用の糸状菌由来セルラーゼを添加して二次加水分解する工程、および
(3)工程(2)の加水分解物を固液分離し、得られた糖液から回収酵素を得る工程。
[2]糖液製造プロセスでの前記工程(1)における糸状菌由来セルラーゼとして、糸状菌由来セルラーゼによるセルロース加水分解物からの回収酵素成分を使用することを特徴とする、[1]記載の糖液の製造方法。
[3]前記工程(1)または(2)における糸状菌由来セルラーゼがトリコデルマ属微生物の培養液由来成分を含むことを特徴とする、[1]または[2]に記載の糖液の製造方法。
[4]前記回収酵素がキシラナーゼおよび/またはキシロシダーゼを含むことを特徴とする、[1]から[3]いずれかに記載の糖液の製造方法。
[5]前記回収酵素が水不溶性の糸状菌由来セルラーゼを含むことを特徴とする、[1]から[4]いずれかに記載の糖液の製造方法。
[6]前記セルロースが、セルロース含有バイオマスをアルカリ処理、水熱処理または希硫酸処理して得られた処理物であることを特徴とする、[1]から[5]いずれかに記載の糖液の製造方法。
[7]一次加水分解および二次加水分解における酵素添加量が、工程(1)での回収酵素の添加量>工程(2)での未使用酵素量の添加量、の関係式を満たすことを特徴とする、[1]から[6]のいずれかに記載の糖液の製造方法。
[8]前記工程(3)における糸状菌由来セルラーゼの回収が、糖液を限外濾過膜に通じてろ過し、非透過側から回収することを特徴とする、[1]から[7]のいずれかに記載の糖液の製造方法。
[9]セルロースを加水分解する工程を含む糖液の製造方法のための装置であって、回収酵素投入配管および未使用酵素投入配管が連結した加水分解槽、加水分解物を固液分離する装置、限外濾過膜の洗浄および/または循環配管内に保持された回収酵素を除去するための水供給配管を有する糖液保持タンク、ならびに酵素と糖液を分離するための限外濾過膜装置、を構成として含む装置。
[10]セルロースを加水分解する工程を含む糖液の製造方法のための装置であって、回収酵素とセルロースを混合し一次加水分解を行うためのセルロース/回収酵素混合装置、セルロース/回収酵素混合物供給配管と未使用酵素投入口が連結した加水分解槽、加水分解物を固液分離する装置、限外濾過膜の洗浄および/または循環配管内に保持された回収酵素を除去するための水供給配管を有する糖液保持タンク、および酵素と糖液を分離するための限外濾過膜装置、を構成として含む装置。
[11][9]または[10]に記載の装置構成に加え、さらに糖液を濃縮する逆浸透膜および/またはナノろ過膜装置、を構成として含む装置。
トリコデルマ培養液由来酵素組成物は以下の方法で調整した。
コーンスティップリカー5%(w/vol)、グルコース2%(w/vol)、酒石酸アンモニウム0.37%(w/vol)、硫酸アンモニウム0.14(w/vol)、リン酸二水素カリウム0.2%(w/vol)、塩化カルシウム二水和物0.03%(w/vol)、硫酸マグネシウム七水和物0.03%(w/vol)、塩化亜鉛0.02%(w/vol)、塩化鉄(III)六水和物0.01%(w/vol)、硫酸銅(II)五水和物0.004%(w/vol)、塩化マンガン四水和物0.0008%(w/vol)、ホウ酸0.0006%(w/vol)、七モリブデン酸六アンモニウム四水和物0.0026%(w/vol)となるよう蒸留水に添加し、100mLを500mLバッフル付き三角フラスコに張り込み、121℃で15分間オートクレーブ滅菌した。放冷後、これとは別にそれぞれ121℃で15分間オートクレーブ滅菌したPE-MとTween80をそれぞれ0.01%(w/vol)添加した。この前培養培地にトリコデルマ・リーセイATCC68589を1×105個/mLになるように植菌し、28℃、72時間、180rpmで振とう培養し、前培養とした(振とう装置:TAITEC社製 BIO-SHAKER BR-40LF)。
コーンスティップリカー5%(w/vol)、グルコース2%(w/vol)、セルロース(アビセル)10%(w/vol)、酒石酸アンモニウム0.37%(w/vol)、硫酸アンモニウム0.14%(w/vol)、リン酸二水素カリウム 0.2%(w/vol)、塩化カルシウム二水和物0.03%(w/vol)、硫酸マグネシウム七水和物0.03%(w/vol)、塩化亜鉛0.02%(w/vol)、塩化鉄(III)六水和物0.01%(w/vol)、硫酸銅(II)五水和物0.004%(w/vol)、塩化マンガン四水和物0.0008%(w/vol)、ホウ酸0.0006%(w/vol)、七モリブデン酸六アンモニウム四水和物0.0026%(w/vol)となるよう蒸留水に添加し、2.5Lを5L容撹拌ジャー(ABLE社製 DPC-2A)容器に張り込み、121℃で15分間オートクレーブ滅菌した。放冷後、これとは別にそれぞれ121℃で15分間オートクレーブ滅菌したPE-MとTween80をそれぞれ0.1%添加し、あらかじめ前記の方法にて液体培地で前培養したトリコデルマ・リーセイATCC68589を250mL接種した。その後、28℃、87時間、300rpm、通気量1vvmにて培養を行い、遠心分離後、上清を膜濾過(ミリポア社製 ステリカップ-GV 材質:PVDF)した。この前述条件で調整した培養液に対し、βグルコシダーゼ(Novozyme188)をタンパク質重量比として、1/100量添加し、これをトリコデルマ由来セルラーゼとして、以下実施例に使用した。
[セルロース前処理物1の調整]
市販のアビセル(メルク社製)を、特段の処理を行うことなくセルロース前処理物1として以下の実施例に使用した。
セルロース含有バイオマスとして、稲藁を使用した。セルロース含有バイオマスを硫酸1%水溶液に浸し、150℃で30分オートクレーブ処理(日東高圧製)した。処理後、固液分離を行い、硫酸水溶液(以下、希硫酸処理液)と硫酸処理セルロースに分離した。次に硫酸処理セルロースと固形分濃度が10重量%となるように希硫酸処理液と攪拌混合した後、水酸化ナトリウムによって、pHを5付近に調整した。これをセルロース前処理物2として以下の実施例に使用した。
セルロースとして、稲藁を使用した。前記セルロース含有バイオマスを小型反応器(耐圧硝子工業製、TVS-N2 30ml)に投入し、液体窒素で冷却した。この反応器にアンモニアガスを流入し、試料を完全に液体アンモニアに浸漬させた。リアクターの蓋を閉め、室温で15分ほど放置した。次いで、150℃のオイルバス中にて1時間処理した。処理後、反応器をオイルバスから取り出し、ドラフト中で直ちにアンモニアガスをリーク後、さらに真空ポンプで反応器内を10Paまで真空引きし乾燥させた。これをセルロース前処理物3として以下実施例に使用した。
セルロース含有バイオマスとして、稲藁を使用した。セルロース含有バイオマスを水に浸し、撹拌しながら180℃で20分間オートクレーブ処理(日東高圧株式会社製)した。その際の圧力は10MPaであった。処理後は溶液成分(以下、水熱処理液)と処理バイオマス成分に遠心分離(3000G)を用いて固液分離した。これをセルロース前処理物4として以下の実施例に使用した。
糖水溶液に含まれるグルコースおよびキシロース濃度は、下記に示すHPLC条件で、標品との比較により定量した。
移動相:ミリQ:アセトニトリル=25:75(流速0.6mL/min)
反応液:なし
検出方法:RI(示差屈折率)
温度:30℃。
トリコデルマ由来セルラーゼの酵素活性は以下手順で測定した。
1)結晶セルロース分解活性
酵素液(所定条件で調整)に対し、アビセル(メルク社製・・要確認)を1g/L、酢酸ナトリウム緩衝液(pH 5.0)を100mMとなるよう添加し、50℃で24時間反応させた。反応液は1mLチューブで調整し、前記条件にて回転混和しながら反応を行った。反応後、チューブを遠心分離し、その上清成分のグルコース濃度を測定した。グルコース濃度は、参考例3に記載の方法に準じて測定した。アビセル分解活性は、生成したグルコース濃度(g/L)をそのまま活性値とした。
酵素液に対し、セロビオース(和光純薬)500mg/L、酢酸ナトリウム緩衝液(pH 5.0)を100mMとなるよう添加し、50℃で0.5時間反応させた。反応液は1mLチューブで調整し、前記条件にて回転混和しながら反応を行った。反応後、チューブを遠心分離し、その上清成分のグルコース濃度を測定した。グルコース濃度は、参考例3に記載の方法に準じて測定した。セロビオース分解活性は、生成したグルコース濃度(g/L)をそのまま活性値とした。
3)キシラン分解活性
酵素液に対し、キシラン(Birch wood xylan、和光純薬)10g/L、酢酸ナトリウム緩衝液(pH 5.0)を100mMとなるよう添加し、50℃で4時間反応させた。反応液は1mLチューブで調整し、前記条件にて回転混和しながら反応を行った。反応後、チューブを遠心分離し、その上清成分のキシロース濃度を測定した。キシロース濃度は、参考例3に記載の方法に準じて測定した。キシロース分解活性は、生成したキシロース濃度(g/L)をそのまま活性値とした。
以下比較例として、一次加水分解および二次加水分解を実施せずにセルロースから糖液を製造した。
セルロース前処理物1~4(各1g)に、参考例1記載の未使用酵素(タンパク質濃度50mg/mL)を0.2mL(タンパク質量10mg)、さらに後述、工程2の手順で回収した回収酵素溶液を混合し添加した。添加した溶液の重量が10gとなるようさらに蒸留水を添加した。本組成物を枝付反応容器に移し(東京理化社製 φ30 NS14/23)、50℃にて19時間保温および攪拌し加水分解を行った(東京理化社製:小型メカニカルスターラー CPS-1000、変換アダプター、三方コック付添加口、保温装置 MG-2200)。
工程1の加水分解物を遠心分離(4500G、10分)にて固液分離し、糖液と残さに分離した。糖液のグルコースおよびキシロース濃度は、参考例3記載の方法で測定し、生成糖として算出した。
セルロース前処理物1~4(各1g)に、未使用酵素(タンパク質濃度50mg/mL)を0.3mL(タンパク質量15mg)添加した(0回目なので回収酵素は添加しない)。添加した溶液の重量が10gとなるようさらに蒸留水を添加した。本組成物を枝付反応容器に移し(東京理化社製 φ30 NS14/23)、50℃にて19時間保温および攪拌し加水分解を行った(東京理化社製:小型メカニカルスターラー CPS-1000、変換アダプター、三方コック付添加口、保温装置 MG-2200)。得られた加水分解物は前述、工程2記載の方法で分離し、回収酵素を得た。またこのときの糖液のグルコースおよびキシロース濃度の測定を行った。
以下実施例として、セルロースの一次加水分解および二次加水分解を行うことにより糖液を製造した。
セルロース前処理物1~4(各1g)に蒸留水を加え、後述、工程3の手順で回収した回収酵素を添加し、総重量が10gとなるようさらに蒸留水を添加した。本組成物を枝付試験管に移し(東京理化社製 φ30 NS14/23)、本組成物を枝付反応容器に移し(東京理化社製 φ30 NS14/23)、50℃にて1時間保温および攪拌し加水分解を行った(東京理化社製:小型メカニカルスターラー CPS-1000、変換アダプター、三方コック付添加口、保温装置 MG-2200)。
工程1の一次加水分解物に、参考例1記載の未使用酵素(タンパク質濃度50mg/mL)を0.2mL(タンパク質量10mg)を添加し、50℃にて18時間反応した。
工程3の二次加水分解物を遠心分離(4500G、10分)にて固液分離し、糖液と残さに分離した。糖液のグルコースおよびキシロース濃度は、参考例3記載の方法で測定し、N回目の生成糖として算出した。さらに糖液を膜濾過(ミリポア社製 ステリフリップ-GP、材質:PES)し、得られた上清は、分画分子量10000の限外濾過膜(Sartorius stedim biotech社製 VIVASPIN 20、材質:PES)を利用し、膜画分が1mLになるまで4500Gにて遠心した。蒸留水10mLを膜画分に添加し、再度膜画分が1mLになるまで4500Gにて遠心した。この後、膜画分から酵素を回収し、これを回収酵素とした。回収酵素は前述、工程1の加水分解に再利用した。
セルロース前処理物1~4(各1g)に、未使用酵素(タンパク質濃度50mg/mL)を0.3mL(タンパク質量15mg)添加した(0回目なので回収酵素は添加しない)。添加した溶液の重量が10gとなるようさらに蒸留水を添加した。本組成物を枝付反応容器に移し(東京理化社製 φ30 NS14/23)、50℃にて19時間保温および攪拌し加水分解を行った(東京理化社製:小型メカニカルスターラー CPS-1000、変換アダプター、三方コック付添加口、保温装置 MG-2200)。得られた加水分解物は前述、工程3記載の方法で分離し、回収酵素を得た。またこのときの糖液のグルコースおよびキシロース濃度の測定を行った。
実施例1において、一次加水分解に添加される回収酵素のタンパク質濃度をBCA測定キット(BCA Protein Assay Regent Kit、ピアス社)を使用して行い、牛アルブミン(2mg/mL)を標品として、562nmの吸光度を測定し、比色定量を行うことにより測定した。セルロース前処理物2に関して、回収再利用を行い回収回数N回目の回収酵素の添加量と、未使用酵素の添加量の関係を表3にまとめた。実施例1表2のグルコース生成量と比較すると、回収再利用回収4回目以降、すなわち、一次加水分解での酵素添加量>二次加水分解での酵素添加量、さらには一次加水分解に再利用する回収酵素添加量>二次加水分解での未使用酵素添加量の関係性が成り立つとき、さらにグルコース生成量がさらに向上することが本実施例で確認できた。
セルロース前処理物3に関して、回収酵素活性(比較例1:回収酵素と新規酵素を同時投入した場合、実施例1:回収酵素を添加し、一次加水分解を行った後、新規酵素を投入した場合)を測定した。酵素活性は、参考例3に準じて、1)結晶セルロース分解活性、2)セロビオース分解活性、3)キシラン分解活性、の3種の分解活性に分けて測定した。各分解活性に関しては、未使用酵素(10mg)における酵素活性を100(%)として、回収酵素における酵素活性を相対値(%)として示した。回収回数2回目と4回目の回収酵素活性に関して、表4(実施例1)、表5(比較例1)に示す。
(実施例4)回収酵素に含まれる凝集したトリコデルマ由来セルラーゼ成分
回収回数4回目以降において、限外濾過膜の非透過液として回収される回収酵素成分に水不溶性の成分が生成することが判明した。この水不溶性のトリコデルマ由来セルラーゼ成分を以下の手順で分析を行った。
(実施例5)水不溶性のトリコデルマ由来セルラーゼ成分の回収酵素成分としての効果
実施例1(セルロース前処理物3)の工程3において、膜画分から酵素を回収し、回収酵素を得たのち、さらに回収酵素を15000rpmで5分遠心した。得られた上済み液のみを回収酵素として、再利用した場合の糖生成量を実施例1の結果と比較した。すなわち、実施例5においては、回収酵素に含まれる水不溶性のトリコデルマ由来セルラーゼ成分を除去した場合における回収酵素の再利用である。
2 保温設備
3 攪拌翼
4 回収酵素投入配管
5 回収酵素保持タンク
6 未使用酵素投入配管
7 セルロース投入口
8 未使用酵素保持タンク
9 プレスろ過装置
10 コンプレッサー
11 水供給配管
12 糖液保持タンク
13 循環ポンプ
14 限外濾過膜装置
15 循環配管
16 回収酵素配管
17 セルロース/回収酵素混合物供給配管
18 セルロース/回収酵素混合装置
19 固液分離装置
20 固形物排出配管
21 三方バルブ
22 精密ろ過膜装置
23 固液分離ろ液タンク
24 ポンプ
25 精密ろ過膜
26 圧空供給装置
27 逆洗浄ポンプ
28 糖液濃縮タンク
29 高圧ポンプ
30 逆浸透膜および/またはナノろ過膜装置
31 三方バルブ
Claims (11)
- 以下の工程(1)~(3)を含む糖液製造プロセスを繰り返して糖液を製造する方法であって、工程(3)で得られた回収酵素を次回以降の糖液製造プロセスの工程(1)に使用することを特徴とする、糖液の製造方法。
(1)セルロースに糸状菌由来セルラーゼを添加し一次加水分解する工程、
(2)工程(1)の加水分解物に未使用の糸状菌由来セルラーゼを添加して二次加水分解する工程、および
(3)工程(2)の加水分解物を固液分離し、得られた糖液から回収酵素を得る工程。 - 糖液製造プロセスでの前記工程(1)における糸状菌由来セルラーゼとして、糸状菌由来セルラーゼによるセルロース加水分解物からの回収酵素成分を使用することを特徴とする、請求項1記載の糖液の製造方法。
- 前記工程(1)または(2)における糸状菌由来セルラーゼがトリコデルマ属微生物の培養液由来成分を含むことを特徴とする、請求項1または2に記載の糖液の製造方法。
- 前記回収酵素がキシラナーゼおよび/またはキシロシダーゼを含むことを特徴とする、請求項1から3いずれかに記載の糖液の製造方法。
- 前記回収酵素が水不溶性の糸状菌由来セルラーゼを含むことを特徴とする、請求項1から4いずれかに記載の糖液の製造方法。
- 前記セルロースが、セルロース含有バイオマスをアルカリ処理、水熱処理または希硫酸処理して得られた処理物であることを特徴とする、請求項1から5いずれかに記載の糖液の製造方法。
- 一次加水分解および二次加水分解における酵素添加量が、工程(1)での回収酵素の添加量>工程(2)での未使用酵素量の添加量、の関係式を満たすことを特徴とする、請求項1から6のいずれかに記載の糖液の製造方法。
- 前記工程(3)における糸状菌由来セルラーゼの回収が、糖液を限外濾過膜に通じてろ過し、非透過側から回収することを特徴とする、請求項1から7のいずれかに記載の糖液の製造方法。
- セルロースを加水分解する工程を含む糖液の製造方法のための装置であって、回収酵素投入配管および未使用酵素投入配管が連結した加水分解槽、加水分解物を固液分離する装置、限外濾過膜の洗浄および/または循環配管内に保持された回収酵素を除去するための水供給配管を有する糖液保持タンク、ならびに酵素と糖液を分離するための限外濾過膜装置、を構成として含む装置。
- セルロースを加水分解する工程を含む糖液の製造方法のための装置であって、回収酵素とセルロースを混合し一次加水分解を行うためのセルロース/回収酵素混合装置、セルロース/回収酵素混合物供給配管と未使用酵素投入配管が連結した加水分解槽、加水分解物を固液分離する装置、限外濾過膜の洗浄および/または循環配管内に保持された回収酵素を除去するための水供給配管を有する糖液保持タンク、および酵素と糖液を分離するための限外濾過膜装置、を構成として含む装置。
- 請求項9または10に記載の装置構成に加え、さらに糖液を濃縮する逆浸透膜および/またはナノろ過膜装置、を構成として含む装置。
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ES11756225.6T ES2689865T3 (es) | 2010-03-15 | 2011-03-14 | Procedimiento para la producción de una solución que contiene azúcar |
EP11756225.6A EP2548966B1 (en) | 2010-03-15 | 2011-03-14 | Manufacturing method for sugar solution |
BR112012022881A BR112012022881B8 (pt) | 2010-03-15 | 2011-03-14 | método para produzir um líquido de açúcar |
CN201180013901.9A CN102791874B (zh) | 2010-03-15 | 2011-03-14 | 糖液的制造方法及其装置 |
US13/634,961 US9624516B2 (en) | 2010-03-15 | 2011-03-14 | Manufacturing method for sugar solution and device for same |
KR1020127025072A KR101778115B1 (ko) | 2010-03-15 | 2011-03-14 | 당액의 제조방법 및 그 장치 |
JP2011513804A JP5970813B2 (ja) | 2010-03-15 | 2011-03-14 | 糖液の製造方法およびその装置 |
CA2792095A CA2792095C (en) | 2010-03-15 | 2011-03-14 | Manufacturing method for sugar solution and device for same |
DK11756225.6T DK2548966T3 (en) | 2010-03-15 | 2011-03-14 | Process for preparing a sugar solution |
RU2012143737/10A RU2564571C2 (ru) | 2010-03-15 | 2011-03-14 | Способ производства раствора сахара и устройство для его производства |
US15/454,242 US10266860B2 (en) | 2010-03-15 | 2017-03-09 | Apparatus that produces sugar solutions from cellulose |
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US13/634,961 A-371-Of-International US9624516B2 (en) | 2010-03-15 | 2011-03-14 | Manufacturing method for sugar solution and device for same |
US15/454,242 Division US10266860B2 (en) | 2010-03-15 | 2017-03-09 | Apparatus that produces sugar solutions from cellulose |
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CN (1) | CN102791874B (ja) |
AU (1) | AU2011228213B2 (ja) |
BR (1) | BR112012022881B8 (ja) |
CA (1) | CA2792095C (ja) |
DK (1) | DK2548966T3 (ja) |
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MY (1) | MY160038A (ja) |
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Also Published As
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BR112012022881B8 (pt) | 2019-06-04 |
BR112012022881B1 (pt) | 2019-05-07 |
CN102791874A (zh) | 2012-11-21 |
ES2689865T3 (es) | 2018-11-16 |
DK2548966T3 (en) | 2018-10-22 |
EP2548966A1 (en) | 2013-01-23 |
KR101778115B1 (ko) | 2017-09-13 |
CA2792095C (en) | 2018-04-24 |
RU2012143737A (ru) | 2014-04-20 |
US20130203117A1 (en) | 2013-08-08 |
RU2564571C2 (ru) | 2015-10-10 |
JP5970813B2 (ja) | 2016-08-17 |
CN102791874B (zh) | 2014-11-26 |
CA2792095A1 (en) | 2011-09-22 |
KR20130038818A (ko) | 2013-04-18 |
EP2548966A4 (en) | 2015-12-16 |
TR201815283T4 (tr) | 2018-11-21 |
AU2011228213B2 (en) | 2014-10-09 |
BR112012022881A2 (pt) | 2015-10-06 |
US10266860B2 (en) | 2019-04-23 |
JPWO2011115040A1 (ja) | 2013-06-27 |
MY160038A (en) | 2017-02-15 |
US20170175065A1 (en) | 2017-06-22 |
US9624516B2 (en) | 2017-04-18 |
EP2548966B1 (en) | 2018-07-18 |
AU2011228213A1 (en) | 2012-10-25 |
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