WO2010027233A2 - Thermococcus supp.로부터 분리된 신규한 수소화효소, 이를 암호화하는 유전자 및 그 유전자를 갖는 미생물을 이용하여 수소를 생산하는 방법 - Google Patents

Thermococcus supp.로부터 분리된 신규한 수소화효소, 이를 암호화하는 유전자 및 그 유전자를 갖는 미생물을 이용하여 수소를 생산하는 방법 Download PDF

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WO2010027233A2
WO2010027233A2 PCT/KR2009/005060 KR2009005060W WO2010027233A2 WO 2010027233 A2 WO2010027233 A2 WO 2010027233A2 KR 2009005060 W KR2009005060 W KR 2009005060W WO 2010027233 A2 WO2010027233 A2 WO 2010027233A2
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WO
WIPO (PCT)
Prior art keywords
gene
thermococcus
same
microorganisms
hydrogen
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PCT/KR2009/005060
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English (en)
French (fr)
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WO2010027233A3 (ko
Inventor
이정현
강성균
이현숙
김상진
권개경
차선신
전정호
조요나
김윤재
배승섭
임재규
정인순
노태옥
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한국해양연구원
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Priority claimed from KR1020080087806A external-priority patent/KR20090060124A/ko
Priority to JP2011525990A priority Critical patent/JP5735425B2/ja
Priority to KR1020137034429A priority patent/KR20140022081A/ko
Priority to KR1020137034431A priority patent/KR101736484B1/ko
Priority to KR1020167020699A priority patent/KR20160108384A/ko
Priority to US12/746,090 priority patent/US8597926B2/en
Application filed by 한국해양연구원 filed Critical 한국해양연구원
Priority to KR1020167023473A priority patent/KR20160114654A/ko
Priority to EP09811732A priority patent/EP2333054A4/en
Priority to KR1020137034430A priority patent/KR101736482B1/ko
Priority to KR1020107013071A priority patent/KR101833975B1/ko
Publication of WO2010027233A2 publication Critical patent/WO2010027233A2/ko
Publication of WO2010027233A3 publication Critical patent/WO2010027233A3/ko
Priority to US14/093,150 priority patent/US9976124B2/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0067—Oxidoreductases (1.) acting on hydrogen as donor (1.12)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P3/00—Preparation of elements or inorganic compounds except carbon dioxide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y112/00—Oxidoreductases acting on hydrogen as donor (1.12)
    • C12Y112/01—Oxidoreductases acting on hydrogen as donor (1.12) with NAD+ or NADP+ as acceptor (1.12.1)
    • C12Y112/01004—Hydrogenase (NAD+, ferredoxin)(1.12.1.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/10—Process efficiency
    • Y02P20/129—Energy recovery, e.g. by cogeneration, H2recovery or pressure recovery turbines
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

Abstract

본 발명은 Thermococcus 속에 속하는 고호열성 신균주로부터 분리한 신규한 수소화효소(hydrogenase), 이를 암호화하는 유전자 및 이들을 이용하여 수소를 생 산하는 방법에 관한 것이다. 본 발명의 수소생산방법에 따르면 상기 균주를 특정 배양조건으로 배양하는 것만으로 많은 양의 수소를 생산할 수 있으므로, 기존의 수 소생산방법에 비하여 경제적이고 효율적이며, 고온에서도 수소를 생산할 수 있는 장점이 있다.
PCT/KR2009/005060 2008-09-05 2009-09-07 Thermococcus supp.로부터 분리된 신규한 수소화효소, 이를 암호화하는 유전자 및 그 유전자를 갖는 미생물을 이용하여 수소를 생산하는 방법 WO2010027233A2 (ko)

Priority Applications (10)

Application Number Priority Date Filing Date Title
KR1020107013071A KR101833975B1 (ko) 2008-09-05 2009-09-07 Ｔｈｅｒｍｏｃｃｕｓ ｓｐｐ．을 이용하여 수소를 생산하는 방법
KR1020137034429A KR20140022081A (ko) 2008-09-05 2009-09-07 개미산을 이용해 수소를 생산해 낼 수 있는 Thermococcus spp. 로부터 분리된 신규한 서열번호 2의 수소화효소, 이를 암호화하는 유전자 및 그 유전자를 갖는 미생물을 이용하여 수소를 생산하는 방법
KR1020137034431A KR101736484B1 (ko) 2008-09-05 2009-09-07 일산화탄소를 이용해 수소를 생산해 낼 수 있는 Thermococcus spp. 로부터 분리된 신규한 수소화효소, 이를 암호화하는 유전자 및 그 유전자를 갖는 미생물을 이용하여 수소를 생산하는 방법
KR1020167020699A KR20160108384A (ko) 2008-09-05 2009-09-07 Ｔｈｅｒｍｏｃｃｕｓ ｓｐｐ．로부터 분리된 신규한 수소화효소 및 이를 이용한 수소를 생산하는 방법
US12/746,090 US8597926B2 (en) 2008-09-05 2009-09-07 Hydrogenases isolated from Thermococcus SPP., genes encoding the same, and methods for producing hydrogen using microorganisms having the genes
JP2011525990A JP5735425B2 (ja) 2008-09-05 2009-09-07 Ｔｈｅｒｍｏｃｏｃｃｕｓ属から分離された新規な水素化酵素、これを暗号化する遺伝子及びその遺伝子を有する微生物を用いて水素を産生する方法
KR1020167023473A KR20160114654A (ko) 2008-09-05 2009-09-07 Ｔｈｅｒｍｏｃｃｕｓ ｓｐｐ．로부터 분리된 신규한 수소화효소 및 이를 이용한 수소를 생산하는 방법
EP09811732A EP2333054A4 (en) 2008-09-05 2009-09-07 NEW, THERMOCOCCUS SPP ISOLATED HYDROGENASE, THESE CODING GENES, AND METHOD FOR PRODUCING HYDROGEN THROUGH MICROORGANISMS WITH THE GENE
KR1020137034430A KR101736482B1 (ko) 2008-09-05 2009-09-07 개미산을 이용해 수소를 생산해 낼 수 있는 Thermococcus spp. 로부터 분리된 신규한 서열번호 7의 수소화효소, 이를 암호화하는 유전자 및 그 유전자를 갖는 미생물을 이용하여 수소를 생산하는 방법
US14/093,150 US9976124B2 (en) 2008-09-05 2013-11-29 Hydrogenase isolated from Thermococcus spp

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
KR10-2008-0087806 2008-09-05
KR20080087794 2008-09-05
KR10-2008-0087794 2008-09-05
KR1020080087806A KR20090060124A (ko) 2007-12-08 2008-09-05 Ｔｈｅｒｍｏｃｏｃｃｕｓ ｓｐｐ.로부터 분리된 신규한 수소화효소, 이를 암호화하는 유전자 및 그 유전자를 갖는 미생물을 이용하여 수소를 생산하는 방법

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US12/746,090 A-371-Of-International US8597926B2 (en) 2008-09-05 2009-09-07 Hydrogenases isolated from Thermococcus SPP., genes encoding the same, and methods for producing hydrogen using microorganisms having the genes
US14/093,150 Division US9976124B2 (en) 2008-09-05 2013-11-29 Hydrogenase isolated from Thermococcus spp

Publications (2)

Publication Number Publication Date
WO2010027233A2 true WO2010027233A2 (ko) 2010-03-11
WO2010027233A3 WO2010027233A3 (ko) 2010-08-19

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PCT/KR2009/005060 WO2010027233A2 (ko) 2008-09-05 2009-09-07 Thermococcus supp.로부터 분리된 신규한 수소화효소, 이를 암호화하는 유전자 및 그 유전자를 갖는 미생물을 이용하여 수소를 생산하는 방법

Country Status (6)

Country Link
US (3) US8597926B2 (ko)
EP (1) EP2333054A4 (ko)
JP (3) JP5735425B2 (ko)
KR (9) KR20110093912A (ko)
RU (2) RU2460789C2 (ko)
WO (1) WO2010027233A2 (ko)

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RU2460789C2 (ru) 2008-09-05 2012-09-10 Корея Оушн Рисерч Энд Девелопмент Инститьют Новая гидрогеназа seq id no:5, очищенная из thermococcus onnurienus na1 с помощью моноokcида углерода, кодирующие ее гены, и способы получения водорода с использованием микроорганизма, имеющего указанные гены
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KR101736485B1 (ko) 2013-03-28 2017-05-16 한국해양과학기술원 포름산염으로부터 수소생산능이 증가된 써모코코스 돌연변이체 및 이를 이용한 수소생산방법
KR101580488B1 (ko) 2013-11-13 2015-12-28 한국해양과학기술원 써모코커스 온누리누스 mc02 및 이를 이용한 수소생산방법
KR101534483B1 (ko) * 2013-11-13 2015-07-07 한국해양과학기술원 써모코커스 온누리누스 wtc155t 균주 및 이를 이용한 수소생산방법
KR101732881B1 (ko) 2014-07-18 2017-05-08 한국과학기술연구원 개미산으로부터의 수소 발생 방법 및 장치
JP6181678B2 (ja) 2015-01-29 2017-08-16 トヨタ自動車株式会社 車両制動制御装置
WO2018008786A1 (ko) * 2016-07-08 2018-01-11 한국해양과학기술원 고농도의 써모코커스 온누리누스 480t 균주 및 이를 이용한 수소생산방법
KR102269939B1 (ko) * 2016-12-09 2021-06-25 한국해양과학기술원 포름산 트랜스포터에서 돌연변이를 가진 써모코코스 온누리에누스 wtf-156t 및 이를 이용한 수소 생산 방법
KR102192800B1 (ko) * 2018-11-12 2020-12-21 한국해양과학기술원 전자전달 체인으로 작동하는 Fe-S 융합단백질 및 이의 용도
KR102050061B1 (ko) 2019-05-28 2019-11-28 (주)경동엔지니어링 미생물을 이용한 수소 가스 연속 생산 플랜트의 모니터링과 운전 안내 장치 및 방법
US11794893B2 (en) 2020-09-08 2023-10-24 Frederick William MacDougall Transportation system for transporting organic payloads
WO2022055827A1 (en) 2020-09-08 2022-03-17 Frederick William Macdougall Coalification and carbon sequestration using deep ocean hydrothermal borehole vents

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JP2012501645A (ja) 2012-01-26
EP2333054A4 (en) 2012-09-12
EP2333054A2 (en) 2011-06-15
KR20160114654A (ko) 2016-10-05
KR20110093913A (ko) 2011-08-18
KR20110069744A (ko) 2011-06-23
US9267115B2 (en) 2016-02-23
JP5735425B2 (ja) 2015-06-17
KR20140022082A (ko) 2014-02-21
US8597926B2 (en) 2013-12-03
RU2010119233A (ru) 2011-12-10
US20100311142A1 (en) 2010-12-09
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RU2012103674A (ru) 2013-08-10
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US9976124B2 (en) 2018-05-22
KR20160108384A (ko) 2016-09-19
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RU2460789C2 (ru) 2012-09-10
KR20110093912A (ko) 2011-08-18
US20140178960A1 (en) 2014-06-26
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